Nanomechanical characterization of soft nanomaterial using atomic force microscopy by Chi-Dat Lam, Soyeun Park

“…Additionally, the establishment of physical or chemical bonds during indentation experiments, observable in retract f-d curves, is crucial for the adhesive properties of samples and binding affinity between antibodies (receptors) and antigens (ligands). Chemical force microscopy, single-molecule force spectroscopy, and single-cell force spectroscopy are primary AFM methods that provide a comprehensive view of such properties through retract curve analysis.Furthermore, this paper, structured into key thematic sections, also reviews the exemplary application of AFM across multiple scientific disciplines. …”
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Metabolic diseases in cattle by V. A. Mischenko, A. V. Mischenko, R. V. Yashin, V. A. Yevgrafova, T. B. Nikeshina

“…The results of epidemiological investigations and laboratory testing of sera samples showed that emulsion inactivated vaccines administered to immunodeficient cattle induce higher titres of virus-specific antibodies than those in animals vaccinated with adsorbed vaccines.…”
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Deglycosylation of Excretory-Secretory Antigens of the Second-Stage Larvae of Toxocara cati Improves Its Efficacy in the Diagnosis of Human Toxocariasis by Ali Pouryousef, Bahador Sarkari, Amir Mootabi Alavi, Mostafa Omidian, Fattaneh Mikaeili

“…The TES antigens, along with the dTES antigens, were used in an ELISA as well as a western blotting system for the detection of anti-Toxocara antibodies. Sera samples collected from 30 confirmed cases of toxocariasis, 30 patients with other diseases, and 30 healthy subjects were evaluated by both systems. …”
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Rh and Kell phenotyping in voluntary blood donors: A study from a tertiary care blood center of western India by Sangita D. Shah, Nidhi M. Bhatnagar, Mamta C. Shah, Garima H. Thakkar, Ujjwal Ahuja, Anu Patel, Drashti Gajera, Priya G. Kalavadiya

“…Cases have been reported where IgG-type of antibodies against Rh and Kell antigens are found which are responsible for transfusion reactions and hemolytic disease of newborn. …”
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Seroprevalence of Peste des Petits Ruminants and Contagious Caprine Pleuropneumonia Coinfections in Goats in Kwale County, Kenya by George Lugonzo, George Gitao, Lilly Bebora, Harrison Osundwa Lutta

“…A total of 368 serum samples were collected from goats of various ages and sexes exhibiting respiratory distress in the four subcounties of Kwale County (Kinango, Lunga Lunga, Matuga, and Msambweni) and screened for PPR and CCPP antibodies. Of the 368 goats sampled, 259 (70.4%) were females and 109 (29.6%) were males, and 126 (34.2%), 71 (19.3%), 108 (29.3%), and 63 (17.1%) samples were collected from Kinango, Matuga, Lunga Lunga, and Msambweni, respectively. …”
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Seroprevalence and Risk Factors of Toxoplasma gondii Infection among Domestic Ruminants in East Hararghe Zone of Oromia Region, Ethiopia by Berhanu Tilahun, Yacob Hailu Tolossa, Getachew Tilahun, Hagos Ashenafi, Shihun Shimelis

“…Sera of 1360 domestic ruminants were analyzed for the presence of anti-T. gondii IgG antibodies using the indirect enzyme-linked immunosorbent assay (iELISA). …”
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Disomic chromosome 3R(3B) substitution causes a complex of meiotic abnormalities in bread wheat <i>Triticum aestivum</i> L. by А. А. Zhuravleva, О. G. Silkova

“…Employing immunostaining with antibodies against microtubule protein, α-tubulin, and the centromerespecific histone (CENH3), as well as FISH, we analyzed microtubule cytoskeleton dynamics and wheat and rye 3R chromosomes behavior in 3R(3B) (Triticum aestivum L. variety Saratovskaya 29 × Secale cereale L. variety Onokhoiskaya) meiosis. …”
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Construction of a Vero cell line expression human KREMEN1 for the development of CVA6 vaccines by Dongqing Zhang, Yuxiang Zou, Jiaying Wu, Longfa Xu, Zhifeng Ke, Yuanyuan Wu, Zhenhong Zhou, Mujin Fang, Ling Chen, Henggang Xu, Jianping Chu, Ningshao Xia, Rui Zhu, Tong Cheng

“…Moreover, the purified CVA6 particles produced from Vero-KRM1_#11 or RD cells both could induce high and comparable levels of IgG and neutralizing antibodies. Importantly, passive transfer of the antisera from CVA6-vaccined mice showed 100% preventive efficacy against CVA6 infection in mice. …”
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Increased Circulating CD4+CXCR5+ Cells and IgG4 Levels in Patients with Myelodysplastic Syndrome with Autoimmune Diseases by Na Xiao, Xin He, Haiyue Niu, Hong Yu, Ningbo Cui, Hongzhao Li, Li Yan, Zonghong Shao, Limin Xing, Huaquan Wang

“…Follicular helper T (Tfh) cells help B cells produce antibodies. The role of Tfh in MDS with AI has not been studied. …”
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Hantavirus and Leptospira are important causes of nonspecific acute febrile syndrome, Meta, Colombia by Liliana Sánchez-Lerma, Salim Mattar, Verónica Contreras, Jorge Miranda, Vaneza Tique, Virginia Rodríguez, Derly Rodriguez, Sonia Lopez, Andrés Rojas-Gulloso

“…Results: Of the study's total of one hundred patients, 14 % showed antibodies against hantavirus IgG, of which two were seroconverted. …”
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Secondary Circulating Prostate Cells Predict Biochemical Failure in Prostate Cancer Patients after Radical Prostatectomy and without Evidence of Disease by Nigel P. Murray, Eduardo Reyes, Nelson Orellana, Cynthia Fuentealba, Leonardo Bádinez, Ruben Olivares, José Porcell, Ricardo Dueñas

“…Serial blood samples were taken during followup after RP, mononuclear cells were obtained by differential gel centrifugation, and CPCs identified using standard immunocytochemistry using anti-PSA monoclonal antibodies. Age, pathological stage (organ confined, nonorgan confined), pathological grade, margin status (positive, negative), extracapsular extension, perineural, vascular, and lymphatic infiltration (positive, negative) were compared with the presence/absence of CPCs and with and without biochemical failure. …”
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Association of Structural Maintenance of Chromosome-1A Phosphorylation with Progression of Breast Cancer by Sushma Yadav, Claudia M. Kowolik, Daniel Schmolze, Yuan Yuan, Min Lin, Arthur D. Riggs, David A. Horne

“…Immunohistochemical (IHC) staining was conducted using previously validated phospho-SMC1A antibodies on a histological section and tissue microarray (TMA) comprising samples from primary, invasive, and metastatic breast cancer and normal breast tissues. …”
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An updated overview of Juvenile systemic sclerosis in a French cohort by Léa Jacquel, Rouba Bechara, Joëlle Terzic, Anne-Cécile Rameau, Emmanuel Chatelus, Linda Rossi-Semerano, Isabelle Kone-Paut, Ulrich Meinzer, Irène Lemelle, Charlotte Rebelle, Diego Urbina, Pascal Pillet, Pauline Choquet, Jad El Maamari, Ariane Zaloszyc, SOFREMIP

“…All children tested positive for antinuclear antibodies, with anti-Scl70 being the most common specificity, even among children with limited cutaneous subsets. …”
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Non-parathyroid hypercalcemia in a patient with new-onset hyperthyroidism and silicone-induced granulomas: case report by Laura Montefusco, Giada Rossi, Iulia Petria, Ida Pastore, Ida Pastore, Paolo Fiorina, Paolo Fiorina, Paolo Fiorina, Paolo Fiorina

“…Biochemical work-up revealed moderate hypercalcemia and overt hyperthyroidism with positive anti-TSH-receptor antibodies. Hydration, loop diuretics and methimazole were initiated immediately. …”
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Flow Cytometric Analysis of T, B, and NK Cells Antigens in Patients with Mycosis Fungoides by Serkan Yazıcı, Emel Bülbül Başkan, Ferah Budak, Barbaros Oral, Şaduman Balaban Adim, Zübeyde Ceylan Kalin, Güven Özkaya, Kenan Aydoğan, Hayriye Saricaoğlu, Şükran Tunali

“…Fluorescent labeled monoclonal antibodies were used to detect cell surface antigens: T cells (CD3+, CD4+, CD8+, TCRαβ+, TCRγδ+, CD7+, CD4+CD7+, CD4+CD7−, and CD71+), B cells (HLA-DR+, CD19+, and HLA-DR+CD19+), NKT cells (CD3+CD16+CD56+), and NK cells (CD3−CD16+CD56+). …”
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Inferior Outcomes of Fludarabine–Cyclophosphamide–Rituximab Chemotherapy in Korean Chronic Lymphocytic Leukemia Patients with Concurrent Thrombocytopenia and Anemia by Tong-Yoon Kim, Gi-June Min, Young-Woo Jeon, Seung-Ah Yahng, Seok-Goo Cho, Jong-Mi Lee, Myungshin Kim, Ki-Seong Eom

“…<b>Background/Objectives</b>: Anti-CD20 monoclonal antibodies combined with alkylator-based chemotherapy enhance survival in chronic lymphocytic leukemia (CLL). …”
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Akt/p27kip1 Pathway Is Not Involved in Human Insulinoma Tumorigenesis by Adriana Graciela Díaz, Andrea Paes de Lima, Paula Garibaldi, Maria de los Milagros Rubio, Florencia García, Marta Kral, Oscar D. Bruno

“…Staining was performed on embedded paraffin tissue using polyclonal antibodies against total Akt, p-Akt, p27kip1, and pp27kip1. p-Akt was the predominant form in insulinomas; they presented lower Akt and p-Akt expression than normal islets in 83.3% and 87.5% of tumors, respectively. p27kip1 and pp27kip1 were mainly cytoplasmic in both insulinomas and normal tissue. …”
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Immunophenotypic Characterization of Human Bone Marrow Mast Cells. A Flow Cytometric Study of Normal and Pathological Bone Marrow Samples by Luis Escribano, Alberto Orfao, Jesús Villarrubia, Beatriz Díaz-Agustín, Carlos Cerveró, Agustín Rios, José L. Velasco, Juana Ciudad, José L. Navarro, Jesús F. San Miguel

“…The goal of the present paper was to define the immunophenotype of bone marrow mast cells (BMMC) from healthy controls and patients with hematologic malignancies (HM) based on the use of multiple stainings with monoclonal antibodies analyzed by flow cytometry. Our results show that BMMC from both groups of individuals display a similar but heterogenous immunophenotype. …”
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Iodine-131 Combined With Plasma Exchange Treatment in Graves’ Hyperthyroidism Patients With Severe Liver Injury Whose Average Model for End-Stage Liver Disease Scores >20 by Xin-Fang Zhu, Rong-Rong Ding, Bing-Yao Wang, Yun-hui Yang, Fu-Xia Ta, Yuan Wang, Qing-Mei Gao, Qi Zhang, Rong Xia, Xing-Guang Luo, Xuan Wang, Jian-Ming Zheng, Hui-Qing Zhu

“…The levels of hemoglobin, platelet count, alanine aminotransferase, aspartate aminotransferase, total bilirubin, direct bilirubin, alkaline phosphatase, glutamyl transpeptidase, MELD scores, free triiodothyronine, free thyroxine, antithyroid peroxidase autoantibody and serum thyrotropin receptor antibodies after PE treatment were significantly lower than before PE treatment (P < .05). …”
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Caerin 1.1/1.9-mediated antitumor immunity depends on IFNAR-Stat1 signalling of tumour infiltrating macrophage by autocrine IFNα and is enhanced by CD47 blockade by Junjie Li, Yuandong Luo, Quanlan Fu, Shuxian Tang, Pingping Zhang, Ian H. Frazer, Xiaosong Liu, Tianfang Wang, Guoying Ni

“…In this study, we explored the impact of F1/F3 on the tumor microenvironment in a transplanted B16 melanoma model, and further investigated the mechanism of action of F1/F3 using monoclonal antibodies to deplete relevant cells, gene knockout mice and flow cytometry. …”
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