The Association of Autoantibodies in Hyperthyroid Heart Disease Combined with Pulmonary Hypertension by Xiujuan Zhang, Lin Chen, Jianping Sheng, Chaoying Li, Yong He, WenXia Han

“…According to the Independent samples T-test, variance analysis, chi-square test, Pearson linear correlation analysis, and logistic regression, there was a good correlation between pulmonary artery systolic pressure and thyroid stimulating hormone (TSH) and receptor antibodies (TRAb) (r = 0.264, P=0.025) (OR = 1.037, P=0.029), but there was no significant correlation between the pulmonary artery systolic pressure and other thyroid-related parameters (FT3, FT4, TSH, anti-TPO, and anti-TG). …”
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Seroprevalence of Herpes Simplex Virus Infection in HIV Coinfected Individuals in Eastern India with Risk Factor Analysis by Soumyabrata Nag, Soma Sarkar, Debprasad Chattopadhyay, Sanjoy Bhattacharya, Rahul Biswas, Manideepa SenGupta

“…Serum was tested for IgM and IgG antibodies of both HSV-1 and HSV-2 by ELISA. The seroprevalence was compared among the three groups of study population, considering the demographic and socioeconomic parameters. …”
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HDAC1-3 inhibition triggers NEDD4-mediated CCR2 downregulation and attenuates immunosuppression in myeloid-derived suppressor cells by Zhiqi Xie, Jinjin Shao, Zeren Shen, Zhichao Ye, Yoshiaki Okada, Daisuke Okuzaki, Naoki Okada, Masashi Tachibana

“…Our results also suggest that HDAC1-3 inhibitors potentiate the antitumor effects of anti-programmed cell death protein 1 antibodies. ATAC-seq and RNA-seq analyses revealed 115 genes epigenetically upregulated by HDAC1-3 inhibitors, primarily linked to transcriptional regulation and ubiquitination. …”
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Increased Death of Peripheral Blood Mononuclear Cells after TLR4 Inhibition in Sepsis Is Not via TNF/TNF Receptor-Mediated Apoptotic Pathway by Chien-Ming Chu, Li-Chung Chiu, Chung-Chieh Yu, Li-Pang Chuang, Kuo-Chin Kao, Li-Fu Li, Huang-Pin Wu

“…Stimulated peripheral blood mononuclear cells (PBMCs) with or without lipopolysaccharides (LPS) and high-mobility group box 1 (HMGB1) were cultured with or without TLR4 inhibition using monoclonal antibodies from 20 patients with sepsis. Caspase-3, caspase-8, and caspase-9 activities were measured. …”
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The Role of Macrophages in the Pathogenesis of Celiac Disease by A. I. Khavkin, E. V. Loshkova, E. I. Kondratieva, N. S. Shapovalova, I. R. Grishkevich, I. V. Doroshenko, G. N. Yankina

“…Celiac disease is an autoimmune disease with a characteristic serological (antibodies to tissue transglutaminase, endomysium, deamidated gliadin peptides) and histological profile (inflammatory infiltration of the villous epithelium by lymphocytes and their atrophy, crypt hyperplasia) caused by gluten consumption in genetically predisposed individuals. …”
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Carrageenan-induced acute inflammation in the mouse air pouch synovial model. Role of tumour necrosis factor by M. Romano, R. Faggioni, M. Sironi, S. Sacco, B. Echtenacher, E. Di Santo, M. Salmona, P. Ghezzi

“…We could not measure IL-1 but the production of IL-6 and TNF reached a maximum after 5-24 h then quickly decreased. Anti-TNF antibodies inhibited cell migration by 50% 24 h after treatment. …”
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Impact of Combined Antiretroviral Treatment (cART) on Latent Cytomegalovirus Infection by Aura Temereanca, Luminita Ene, Gratiela Tardei, Camelia Grancea, Cristian L. Achim, Simona Ruta

“…We explored the impact of combination antiretroviral therapy (cART) on latent CMV infection in 225 young adults parenterally infected with HIV during childhood. Anti-CMV IgG antibodies were present in 93.7% of participants, with lower levels correlating with longer cART exposure and better immunologic parameters. …”
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Differential diagnosis of rheumatic manifestations of acromegaly. Case report by Margarita A. Perepelova, Larisa K. Dzeranova, Taras S. Panevin, Elena G. Przhiyalkovskaya, Ekaterina A. Pigarova, Ekaterina A. Troshina, Evgeniy G. Zotkin

“…As part of the examination, an increase in the titer of the antinuclear factor (1/2560) and the level of antibodies to ribonucleoprotein was revealed, which is most typical for mixed connective tissue disease. …”
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Elevated BP180 ELISA at Diagnosis Correlates with Disease Severity and Relapse in Oral Mucous Membrane Pemphigoid: Preliminary Results from a Retrospective Monocentric Italian Stud... by Andrea Gabusi, Davide B. Gissi, Roberto Rossi, Federica Filippi, Camilla Loi, Cosimo Misciali, Giacomo Clarizio, Michelangelo La Placa, Federico Bardazzi

“…The aim of the present study was to investigate if the levels of anti-BP180 antibodies at diagnosis could be correlated with clinical severity and relapse. …”
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Canadian Public Health Laboratory Network laboratory Guidelines for the Use of Serological Tests (excluding point-of-care tests) for the Diagnosis of Syphilis in Canada by Paul N Levett, Kevin Fonseca, Raymond SW Tsang, Kamran Kadkhoda, Bouchra Serhir, Sandra M Radons, Muhammad Morshed

“…Some of the issues related to serological diagnoses are that antibodies take time to appear after infection, and serology screening tests require several secondary confirmatory tests that can produce complex results needing interpretation by experts in the field. …”
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Microscopic Observation of Proliferative Membranes in Fibrocontractive Retinal Disorders by Rino Frisina, Francesco Tessarolo, Ivan Marchesoni, Federico Piccoli, Emiliana Bonomi, Patrizio Caciagli, Edoardo Midena, Giandomenico Nollo

“…Immunohistochemical staining was performed with antibodies to alpha smooth muscle actin and CD68. Data were analyzed after grouping according to the following tissue types: ILM (20 specimens), epiretinal membrane (ERM) (22 specimens), ILM + ERM (20 specimens), and proliferative vitreoretinopathy (PVR) (7 specimens). …”
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Decreased Expression of Thyroglobulin and Sodium Iodide Symporter Genes in Hashimoto’s Thyroiditis by Anna Popławska-Kita, Beata Telejko, Katarzyna Siewko, Maria Kościuszko-Zdrodowska, Natalia Wawrusewicz-Kurylonek, Adam Krętowski, Justyna Hryniewicka, Janusz Dzięcioł, Witold Bauer, Robert Milewski, Małgorzata Szelachowska, Maria Górska

“…In the HT group Tg mRNA expression correlated positively with NIS mRNA expression (R=0.739, P=0.0001) and thyroid volume (R=0.465, P=0.0005), as well as negatively with TNFα mRNA expression (R=-0.490, P=0.001) and anti-peroxidase antibodies (TPOAb) level (R=-0.482, P=0.0002), whereas NIS mRNA expression correlated positively with thyroid volume (R=0.319, P=0.02), as well as negatively with TNFα mRNA expression (R=-0.529, P=0.0006) and TPOAb level (R=-0.422, P=0.001). …”
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Immunogenicity of HIV-1 <i>Env</i> mRNA and <i>Env-Gag</i> VLP mRNA Vaccines in Mice by Qi Ma, Jing Yang, Xiaoguang Zhang, Hongxia Li, Yanzhe Hao, Xia Feng

“…HIV Env-specific binding and neutralizing antibodies in mouse sera were assessed via enzyme-linked immunosorbent assay (ELISA) and pseudovirus-based neutralization assays, respectively. …”
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Expression, Purification, and Evaluation of Diagnostic Potential and Immunogenicity of a Recombinant NS3 Protein from All Serotypes of Dengue Virus by Laura Mónica Álvarez-Rodríguez, Angel Ramos-Ligonio, José Luis Rosales-Encina, María Teresa Martínez-Cázares, Aurora Parissi-Crivelli, Aracely López-Monteon

“…The fusion proteins were immunogenic, inducing antibodies to be able to recognize to antigens up to a 1 : 3200 dilution. …”
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Progress of CCL20-CCR6 in the airways: a promising new therapeutic target by Ya -Jing Li, Wan-Li Geng, Chen-Chen Li, Jia-Hao Wu, Fei Gao, Yong Wang

“…Numerous studies have demonstrated that therapeutic interventions targeting CCL20 and CCR6, including antibodies and antagonists, have the potential to mitigate disease progression. …”
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Carboxylated Graphene: An Innovative Approach to Enhanced IgA-SARS-CoV-2 Electrochemical Biosensing by Luciana de Souza Freire, Ariamna María Dip Gandarilla, Yonny Romaguera Barcelay, Camila Macena Ruzo, Barbara Batista Salgado, Ana P. M. Tavares, Francisco Xavier Nobre, Julio Nino de Souza Neto, Spartaco Astolfi-Filho, Ștefan Țălu, Pritesh Lalwani, Niranjan Patra, Walter Ricardo Brito

“…We modified screen-printed carbon electrodes (SPCEs) with CG to immobilize the SARS-CoV-2 N-protein, facilitating targeted detection of IgA antibodies (IgA-SARS-CoV-2). The analytical performance was assessed via electrochemical techniques such as cyclic voltammetry and electrochemical impedance spectroscopy, confirming CG synthesis effectiveness and biosensor functionality. …”
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Recombinant Enolase of Trypanosoma cruzi as a Novel Vaccine Candidate against Chagas Disease in a Mouse Model of Acute Infection by Minerva Arce-Fonseca, María Cristina González-Vázquez, Olivia Rodríguez-Morales, Verónica Graullera-Rivera, Alberto Aranda-Fraustro, Pedro A. Reyes, Alejandro Carabarin-Lima, José Luis Rosales-Encina

“…Our results showed that mice vaccinated with rTcENO or its encoding DNA were able to generate typical specific antibodies (IgG1, IgG2a, and IgG2b), suggesting that a mixed Th1/Th2 immune response was induced. …”
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Quantitative fluorescent nanoparticle tracking analysis and nano‐flow cytometry enable advanced characterization of single extracellular vesicles by Danilo Mladenović, Joseph Brealey, Ben Peacock, Kairi Koort, Nataša Zarovni

“…This standardization technique allowed for fluorescent EV signal to be expressed in ERF units (indicative of bound fluorescent antibodies per EV), thus enabling measurement of target protein marker abundance on individual EVs, and in the whole EV population. …”
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Dengue Virus NS1 Protein as a Diagnostic Marker: Commercially Available ELISA and Comparison to qRT-PCR and Serological Diagnostic Assays Currently Used by the State of Florida by Jason H. Ambrose, Shamala Devi Sekaran, Azliyati Azizan

“…Here, real-time molecular assays have proven useful but have limitations, whereas ELISAs that detect antibodies are still favored but results are obtained too late to be of clinical value. …”
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Very Early-Onset Inflammatory Bowel Disease (VEO-IBD) Presenting with Recurrent Leukocytoclastic Vasculitis Preceded by Streptococcal Pharyngitis by Ashley Fonseca, Julee Sunny, Lina M. Felipez

“…She was started on infliximab 10 mg/kg every four weeks after infliximab titers, and antibodies were obtained. Currently, the patient remains on clinical and biochemical remission, with no recent LV episodes or recurrence of streptococcal pharyngitis. …”
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