Neonatal Sepsis in a Resource-Limited Setting: Causative Microorganisms and Antimicrobial Susceptibility Profile by Edna Nai Acheampong, Joseph Adjei Tsiase, Daniel Kwame Afriyie, Seth Kwabena Amponsah

“…As there is some level of antibiotic resistance observed in the current study, it is necessary for routine microbial analysis of samples and their antibiogram.…”
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Harnessing CRISPR interference to resensitize laboratory strains and clinical isolates to last resort antibiotics by Angelica Frusteri Chiacchiera, Michela Casanova, Massimo Bellato, Aurora Piazza, Roberta Migliavacca, Gregory Batt, Paolo Magni, Lorenzo Pasotti

“…However, the emergence of target sequence mutations triggered by Cas-cleavage was identified as an escape strategy, posing the risk of generating new antibiotic-resistance gene (ARG) variants. Here, we evaluated an antibiotic re-sensitization strategy based on CRISPR interference (CRISPRi), which inhibits gene expression without damaging target DNA. …”
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Temperate phage-antibiotic synergy is widespread—extending to Pseudomonas—but varies by phage, host strain, and antibiotic pairing by Rabia Fatima, Alexander P. Hynes

“…Overall, our findings indicate that temperate phages can act as adjuvants in clinically relevant pathogens, even in the presence of antibiotic resistance, thereby drastically expanding their therapeutic potential.IMPORTANCEThe recent discovery that otherwise therapeutically unusable temperate phages can potentiate the activity of antibiotics, resulting in a potent synergy, has only been tested in E. coli, and with a single model phage. …”
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Antibiogram Profile of Patients Having Urinary Tract Infection Treated with Most Commonly Used Antibiotics by Bilal Ahmad, Faleesha Gul, Said Hassan, Haseeba Arif, Amber Javaid, Shawkat Ali

“…This study investigated UTI prevalence and antibiotic resistance to inform optimized treatment guidelines. …”
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Antibiotic Use among Patients Visiting Primary Hospitals in Northwest Ethiopia: A Multicenter Cross-Sectional Survey by Adeladlew Kassie Netere, Ashenafi Kibret Sendekie

“…These findings suggest that a greater effort is required in public education related to proper and safe uses of antibiotics and that greater efforts are needed to enforce the control of antibiotic use to overcome the emergence of antibiotic resistance.…”
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Decomposition of the pangenome matrix reveals a structure in gene distribution in the Escherichia coli species by Siddharth M. Chauhan, Omid Ardalani, Jason C. Hyun, Jonathan M. Monk, Patrick V. Phaneuf, Bernhard O. Palsson

“…This approach reveals the genetic basis for differential traits across phylogroups, offering insights into pathogenicity, antibiotic resistance, and evolutionary adaptations. The findings enhance the potential for genome-based diagnostics and pave the way for future studies aimed at achieving a global genetic definition of bacterial phylogeny.…”
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Novel silver nanoparticle-based biomaterials for combating Klebsiella pneumoniae biofilms by Eslam Elashkar, Rihaf Alfaraj, Ola M. El-Borady, Mahmoud M. Amer, Abdelazeem M. Algammal, Azza S. El-Demerdash

“…This study investigated the efficacy of a novel nanoformulation, AgNPs@chitosan-NaF, in combating K. pneumoniae biofilms.MethodsAntimicrobial susceptibility testing was performed to assess the antibiotic resistance profile of K. pneumoniae isolates. …”
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Dietary supplementation with Jasminum sambac leaf powder: Effects on growth, hematology, and immune parameters in broiler chickens by Iram Maqsood, Samreen Sareer, Shafique Ur Rehman, Ayesha Hidayat, Madeeha Shah, Saira Awaz, Rong Ke

“…Nonetheless, problems with industrial mechanization, pollution, animal welfare, and antibiotic resistance affect the poultry industry. The objective of this study was to assess the impact of adding Jasminum sambac leaf powder (JSLP) to the feed of broiler chickens on their growth performance, coliform bacterial levels, and immunological function. …”
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Isolation and Characterization of Potential Antibiotic-Producing Actinomycetes from Water and Soil Sediments of Different Regions of Nepal by Bhanu Shrestha, Dharmendra Kumar Nath, Alina Maharjan, Anju Poudel, Roshani Nhuchhen Pradhan, Sagar Aryal

“…Thus, exploitation of various regions of Nepal for the discovery of an effective antimicrobial agent is helpful in providing a solution to the cost-effective therapy and action against antibiotic resistance.…”
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Retrospective Analysis of Penicillin G Minimum Inhibitory Concentration for Gram-Positive Isolates of Non-Severe Clinical Mastitis by Stefanie Leimbach, Franziska Nankemann, Anne Tellen, Doris Klocke, Nicole Wente, Yanchao Zhang, Volker Krömker

“…<b>Conclusions</b>: The data show that in Northern Germany, it can be assumed that penicillin is a good choice in most cases of mastitis caused by Gram-positive mastitis pathogens, at least from the perspective of antibiotic resistance.…”
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Biomolecule screen identifies several inhibitors of Salmonella enterica surface colonization by Joseph Headrick, Amital Ohayon, Shannon Elliott, Jacob Schultz, Erez Mills, Erik Petersen

“…Salmonella enterica is a foodborne pathogen commonly found in agricultural facilities; its prevalence, as well as increasing levels of disinfectant- and antibiotic-resistance, has significant costs for agriculture as well as human health. …”
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Using the Sleeping Beauty Transposon System for Doxycycline-inducible Gene Expression in RAW264.7 Macrophage Cells to Study Phagocytosis by Parsa Kamali, Gregory Fairn

“…Experimentally, the system introduces two plasmids: one carrying the Sleeping Beauty transposase and the other with an integration cassette carrying the gene of interest, a reverse-doxycycline controlled repressor gene, and an antibiotic resistance gene. The construct used in this protocol provides puromycin resistance. …”
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Contributing factors to postoperative surgical site infections in pituitary neuroendocrine tumors undergoing endonasal transsphenoidal resection by Lei Wang, Shanxian Liu, Jie Zheng, Rui Li, Zhenyi Xing

“…Microbial analysis revealed Escherichia coli as the most prevalent pathogen in post-surgery infections, with notable antibiotic resistance patterns observed in key bacteria, necessitating careful antibiotic selection. …”
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Unveiling the nanoworld of antimicrobial resistance: integrating nature and nanotechnology by Devesh Sharma, Devesh Sharma, Sakshi Gautam, Sakshi Singh, Nalini Srivastava, Abdul Mabood Khan, Deepa Bisht

“…A significant global health crisis is predicted to emerge due to antimicrobial resistance by 2050, with an estimated 10 million deaths annually. Increasing antibiotic resistance necessitates continuous therapeutic innovation as conventional antibiotic treatments become increasingly ineffective. …”
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Antimicrobial Evaluation of Bacterial Isolates from Urine Specimen of Patients with Complaints of Urinary Tract Infections in Awka, Nigeria by Perpetua A. Ekwealor, Malachy C. Ugwu, Ifeanyi Ezeobi, George Amalukwe, Belinda C. Ugwu, Ugochukwu Okezie, Catherine Stanley, Charles Esimone

“…It was also observed that all the bacterial species except Streptococcus spp. have a Multiple Antibiotic Resistance Index (MARI) greater than 0.2. For empiric treatment of UTIs in Awka locality, Nitrofurantoin, Ciprofloxacin, and Ofloxacin are the first line of choice.…”
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The Optimal First-Line Therapy of Helicobacter pylori Infection in Year 2012 by Chao-Hung Kuo, Fu-Chen Kuo, Huang-Ming Hu, Chung-Jung Liu, Sophie S. W. Wang, Yen-Hsu Chen, Ming-Chia Hsieh, Ming-Feng Hou, Deng-Chyang Wu

“…First-line therapies are facing a challenge because of increasing treatment failure due to elevated antibiotics resistance. Several new treatment strategies that recently emerged to overcome antibiotic resistance have been surveyed. …”
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Pathogen Detection and Resistome Analysis in Healthy Shelter Dogs Using Whole Metagenome Sequencing by Smriti Shringi, Devendra H. Shah, Kimberly Carney, Ashutosh Verma

“…These bacterial pathogens may also carry antibiotic resistance genes (ARGs), serving as a reservoir for antimicrobial resistance (AMR) transmission. …”
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Antimicrobial resistance characterization of Staphylococcus aureus from different animal food origins in Jimma, South Western Ethiopia by Diriba Taddese, Mukarim Abdurahaman, Motuma Debelo, Eshetu Shumi, Gelana Urgessa, Dase Kefyalew, Moa Melaku, Tadele Kebeta, Gazali Abafaji

“…Kanamycin and tetracycline exhibited the highest resistance rates among the isolates, with 39.0% and 36.6% resistance, respectively. The least antibiotic resistance was observed against cefoxitin (7.3%). …”
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On‐chip label‐free impedance‐based detection of antibiotic permeation by Jaspreet Kaur, Hamed Ghorbanpoor, Yasin Öztürk, Özge Kaygusuz, Hüseyin Avcı, Cihan Darcan, Levent Trabzon, Fatma D. Güzel

“…In particular, with the rise in antibiotic resistance amongst pathogenic bacteria, the study of antibiotic activity and transport across cell membranes in the field of biosensors has been gaining widespread importance. …”
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Antibacterial Activity of Rationally Designed Antimicrobial Peptides by Marius B. Tincho, Thureyah Morris, Mervin Meyer, Ashley Pretorius

“…The reasons may either be the antibiotic resistance towards the available therapeutic molecules or the slow rate of producing adequate therapeutic regimens to tackle the rapid growth of new infectious diseases, as well as the toxicity of current treatment regimens. …”
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