CAM/TMA-DPH as a promising alternative to SYTO9/PI for cell viability assessment in bacterial biofilms by Tinatini Tchatchiashvili, Tinatini Tchatchiashvili, Mateusz Jundzill, Mateusz Jundzill, Mike Marquet, Kamran A. Mirza, Kamran A. Mirza, Mathias W. Pletz, Mathias W. Pletz, Oliwia Makarewicz, Oliwia Makarewicz, Lara Thieme, Lara Thieme

“…To address this limitation, we investigated an alternative staining method employing calcein acetoxymethyl (CAM) to detect viable cells based on esterase activity, and 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene p-toluenesulfonate (TMA-DPH) to assess the remaining biofilm population.MethodsBiofilms of Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, and Enterococcus faecium were matured and exposed to varying concentrations of antibiotics or sterile medium. Biofilm viability was assessed using CAM/TMA-DPH or SYTO9/PIstaining, followed by analysis with confocal laser scanning microscopy (CLSM) and ImageJ-based biofilm surface coverage quantification. …”
Get full text

Informatization of the processes of administration of the quality of medical services in surgical hospitals by Ya.S. Bereznytskyi, V.Ya. Bereznytskyi, O.V. Hudoshyna, N.D. Chuhrienko, G.V. Bereznytska, Ye.V. Zalyhina, Yu.B. Dianova, E.A. Rynska, L.N. Ksykevich

“…The quality control of the use of antibiotics, the frequency of their use for preventive and therapeutic purposes is also carried out. …”
Get full text

Early results of primary total hip arthroplasty performed in young adults at a regional Saudi-Arabian orthopedics center: a prospective observational study by Walid M. Al-Shaer, Ali H. Al-Yami, Mokhtar Ahmed Al-Sayed, Ali Mohammed Al-Zaidan, Emad Ruddah Al-Sufyani, Ahmed A. Khalifa, Raad M. M. Al-Thaqafi, Abdulmohsen H. Al-Hamyani, Mohammed M. Al-Subaie

“…Two (8%) patients had superficial wound infections at the time of suture removal, which were treated successfully by daily dressing and antibiotics. None required revision. Conclusions. …”
Get full text

Bone Defect Repair Using a Bone Substitute Supported by Mesenchymal Stem Cells Derived from the Umbilical Cord by Michal Kosinski, Anna Figiel-Dabrowska, Wioletta Lech, Lukasz Wieprzowski, Ryszard Strzalkowski, Damian Strzemecki, Lukasz Cheda, Jacek Lenart, Krystyna Domanska-Janik, Anna Sarnowska

“…Mesenchymal stem cells derived from the umbilical cord (Wharton’s jelly (WJ-MSC)) were cultured in GMP-grade DMEM low glucose supplemented with heparin, 10% platelet lysate, glucose, and antibiotics. In vitro WJ-MSCs were seeded on the bone substitute Bio-Oss Collagen® and cultured in the StemPro® Osteogenesis Differentiation Kit. …”
Get full text

Impact of Flexor Tendon Traction Tenolysis on Clinical Outcomes in Open A1 Pulley Release for Trigger Finger by Shalimar Abdullah, BMBS, MS, Syed Jeffrey Syed Ahmad Kabeer, MBChB, MD, Lim Chia Hua, MD, Jamari Sapuan, MD, MS, Parminder Singh Gill, MBBS, MS, Elaine Soh Zi Fan, MBBS, MD

“…Two patients in group I experienced wound infections, which resolved with a week-long course of antibiotics. Conclusions: Open A1 pulley release with flexor tendon traction tenolysis resulted in better early postoperative (2 weeks) finger flexion range of motion and QuickDASH scores, albeit with a longer surgery duration. …”
Get full text

Antifungal activity and biocompatibility assessment with molecular docking and dynamic simulations of new pyrazole derivatives by Basma T. Abd-Elhalim, Ghada G. El-Bana, Ahmed F. El-Sayed, Ghada E. Abdel-Ghani

“…Abstract Background Because of their many bioactivities, which include psychoanalytic, antifungal, antihypertensive, anti-inflammatory, and antiviral properties, pyrazoles and their derivatives are attracting interest in pharmacology and medicine, the pressing need for novel fungicides is increased for lessened by the growing microbiological resistance of illnesses to recognized antibiotics. Objective The current work validates the results and pyrazole binding sites as potent antifungals by investigating many pyrazole derivatives as antifungal agents. …”
Get full text

Molecular Detection of Carbapenemase-Encoding Genes in Multidrug-Resistant Acinetobacter baumannii Clinical Isolates in South Africa by Yaw Adjei Anane, Teke Apalata, Sandeep Vasaikar, Grace Emily Okuthe, Sandile Songca

“…Most strains showed high resistance rates (>80%) to the antibiotics tested. Resistance to amikacin, tetracycline, and tigecycline were 50%, 64%, and 48%, respectively. …”
Get full text

Pengaruh Pemberian Glutamin pada Kemampuan Fagositosis Makrofag terhadap Pseudomonas Aeruginosa by Daslina , Eryati Darwin, Aziz Djamal

“…<br />Kata kunci: glutamin, fagositosis makrofag, Pseudomonas aeruginosa</p><p> </p><p>Abstract <br />Pseudomonas aeruginosa is the most common cause of bacterial infections that are resistant to antibiotics. Glutamine is an amino acid  in the body that able to modulate the body's immune function. …”
Get full text

Dissemination of IncQ1 Plasmids Harboring NTE_KPC-IId in a Brazilian Hospital by Camila Maria dos Santos Boralli, Julian Andres Paganini, Rodrigo Silva Meneses, Camila Pacheco Silveira Martins da Mata, Edna Marilea Meireles Leite, Anita C. Schürch, Fernanda L. Paganelli, Rob J. L. Willems, Ilana L. B. C. Camargo

“…In addition to IncQ1 with NTE_KPC, <i>Enterobacter cloacae</i> BHKPC28 contained the <i>mcr-9</i> gene in an IncHI2/IncHI2A conjugative plasmid, which may help the mobility of IncQ1 and the dissemination of two resistance determinants to last-resort antibiotics. Understanding the interaction between plasmids and high-risk lineages can help develop new therapies to prevent the dissemination of resistance traits.…”
Get full text

Tannin alleviated reproductive dysfunction in pregnant ewes infected with Haemonchus contortus by Xin Li, Xin Li, Hai Xiang, Hai Xiang, Rong Liang, Rong Liang, Xinyu Han, Xinyu Han, Rongzhen Zhong, Rongzhen Zhong, Hongyu Liu, Hongyu Liu, Yi Fang, Yi Fang

“…Therefore, tannin is a suitable potential alternative to antibiotics as a feed additive.…”
Get full text

A murine model of acute and prolonged abdominal sepsis, supported by intensive care, reveals time-dependent metabolic alterations in the heart by Bart Jacobs, Inge Derese, Sarah Derde, Sarah Vander Perre, Lies Pauwels, Greet Van den Berghe, Jan Gunst, Lies Langouche

“…Methods In 24-week-old C57BL/6J mice, sepsis was induced by cecal ligation and puncture, followed by intravenous fluid resuscitation, subcutaneous analgesics and antibiotics, and, in the prolonged phase, by parenteral nutrition. …”
Get full text

Distribution of sasX, mupA, and qacA/B genes and determination of genetic relatedness of epidemic methicillin-resistant Staphylococcus aureus strains associated with bloodstream in... by Rui Zhao, Bingyu Du, Lingling Hu, Chenxi Li, Fen Xue, Xing Wang, Changhong Jiang, Jinghua Wang, Yanfeng Zhao

“…The resistance of these strains to 16 antibiotics was also analyzed. The distribution of these genes and their association with epidemic STs were analyzed.ResultsA total of 26 STs were identified, with notable prevalence in five epidemic clones: ST59, ST5, and ST764. …”
Get full text

Factors influencing pregnancy rate following estradiol-free fixed TAI protocols in water buffaloes by Adriana Camacho de Gutiérrez, Obdulio Camacho, Steward Fernández, Yerixo González, Juan Carlos Gutiérrez-Añez

“…Furthermore, animals treated with PGF2α and antibiotics to correct abnormal uterine status showed a higher PR 86% vs 50% than animals with apparently normal uterine status. …”
Get full text

Evaluation of Tumour Necrosis Factor Alpha, Interleukin-2 Soluble Receptor, Nitric Oxide Metabolites, and Lipids as Inflammatory Markers in Type 2 Diabetes Mellitus

“…To restrict the influence of variables that could interfere in the interpretation of data, subjects with obesity and/or acute or chronic inflammatory disease, haemoglobinopathies, recent use of antibiotics, antiinflammatory drugs, and trauma were excluded. …”
Get full text

Antimicrobial Resistance Surveillance of Skin and Soft Tissue Infections: Hospital-Wide Bacterial Species and Antibiograms to Inform Management at a Zonal Tertiary Hospital in Mwan... by Justine BN, Mushi MF, Silago V, Igembe Z, Muyombe J, Kishengena PP, Michael Jnr NS, Maganga MG, Massenga A, Tegete F, Massaga FA, Seni J

“…Revisiting SSTIs treatment guidelines at BMC and inclusion of SSTIs in the on-going AMR surveillance in Tanzania are recommended.Plain Language Summary: Tanzania National Action Plan on combating resistant bugs is exclusively focused on urinary tract infections and blood stream infections despite enormous impact conferred by skin and soft tissue infections.Bugs which are resistant to commonly used antibiotics were found more among patients admitted in the hospital in contrast to those attending outpatient clinics.This study highlights a pressing need to develop treatment guidelines based on generated local research-evidence to foster favorable patients’ outcomes.Keywords: skin and soft tissue infections, antimicrobial resistance, surveillance, Tanzania…”
Get full text

Immunomodulatory and growth-promoting effects of Rauwolfia serpentina root powder in broiler chicks challenged with Salmonella Gallinarum by Yingyu Zhang, Hiba Rehman, Farina Khattak, Maryam Tariq, Bushra Nisar Khan, Sadia Chaman, Ayaesha Riaz, Muhammad Ovais Omer, Aqib Ali, Qamar un Nisa, Muhammad Muddassir Ali, Gulbeena Saleem

“…BackgroundAmid growing concern about antimicrobial resistance due to the irrational use of antibiotics in treating common poultry diseases, particularly Salmonella which is a foodborne pathogen in humans. …”
Get full text

Synthesis of silver Nanoparticles@Carbon Dot nanocomposites using Aegle marmelos and Euphorbia hirta: Anti vibriocidal and antioxidant properties by Gali Raman, Sirangu Joseph, D Ramachandran, Phanindra Babu Kasi, Abhinash Marukurti, Alavala Matta Reddy, P Vijaya Nirmala, V S Manikanta, R. Lohitha Kavya, K Sarojani, Anitha Kadimi, Silpa Rani Medapalli, S B Sonia Shankar, G Dinesh Ratna, K Gayathri, P Tirupathi Rao

“…In spite of the increasing Antimicrobial Resistance (AMR) in pathogenic organisms due to inappropriate usage of conventional antibiotics, there is an urgent need of alternative therapeutic antimicrobials. …”
Get full text

Risk of aortic aneurysm or dissection following use of fluoroquinolones: a retrospective multinational network cohort studyResearch in context by Jack L. Janetzki, Jung Ho Kim, Evan Minty, Jung Ah Lee, Daniel R. Morales, Rohan Khera, Chungsoo Kim, Thamir M. Alshammari, Scott L. DuVall, Michael E. Matheny, Thomas Falconer, Seonji Kim, Thanh-Phuc Phan, Phung-Anh Nguyen, Min-Huei Hsu, Jason C. Hsu, Rae Woong Park, Kenneth K.C. Man, Sarah Seager, Mui Van Zandt, James P. Gilbert, Patrick B. Ryan, Martijn J. Schuemie, Marc A. Suchard, George Hripcsak, Nicole Pratt, Seng Chan You

“…The study included 13,588,837 patients aged 35 or older who initiated systemic fluoroquinolones (FQs) or comparable antibiotics (trimethoprim with or without sulfamethoxazole [TMP] or cephalosporins [CPHs]) for UTI treatment in the outpatient setting between JAN 01, 2010 and DEC 31, 2019. …”
Get full text