Neoandrographolide inhibits mature osteoclast differentiation to alleviate bone loss and treat osteoporosis by Kai Tang, Kai Tang, Kai Tang, Wei Deng, Wei Deng, Wei Deng, Wei Deng, Zhiying Huang, Zhiying Huang, Zhiying Huang, Simin Chen, Simin Chen, Simin Chen, Zilin Zhu, Zilin Zhu, Shukun Lin, Shukun Lin, Lubin Zhong, Lubin Zhong, Quanxin Zheng, Quanxin Zheng, Wenhua Zhao, Wenhua Zhao, Zhida Zhang, Zhida Zhang, Ling Mo, Ling Mo, Ling Mo

“…Through Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Western blot analysis, we examined the relative expression levels of genes and proteins associated with OC differentiation like CTSK,c-Fos,MMP9,NFATc1, and verified the underlying mechanisms. …”
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Investigation in blood-brain barrier integrity and susceptibility to immune cell penetration in a mouse model of Dravet syndrome by Cristina Alonso, Alicia García-Culebras, Valentina Satta, Inés Hernández-Fisac, Álvaro Sierra, José A. Guimaré, Ignacio Lizasoain, Javier Fernández-Ruiz, Onintza Sagredo

“…The elevated number of blood neutrophils did not apparently originate their infiltration into the hippocampus of DS mice as an immunofluorescence analysis indicated, and the same happened in B- and T-cells. …”
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‘Dynamic zero-COVID’ policy and viral clearance during an omicron wave in Tianjin, China: a city-wide retrospective observational study by Qi Wu, Yan Xie, Tao Cheng, Junping Wu, Wentao Jiang, Lixia Shi, Xiaolong Liu, Yu Hu, Yahui Feng, Hongxia Shao, Xiaowen Gong, Zhen Song, Saibing Qi, Xueou Liu, Qiujin Shen, Junren Chen

“…The median interval from hospital admission to viral clearance was 10 days (range 3–28). An exploratory analysis identified a feature cluster associated with earlier viral clearance, with HRs of 3.56 (95% CI 1.66 to 7.63) and 3.15 (95% CI 1.68 to 5.91) in the training and validation sets, respectively.Conclusions The ‘dynamic zero-COVID’ policy can suppress an omicron wave within a month. …”
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Tadalafil treatment for fetuses with early-onset growth restriction: a protocol for a multicentre, randomised, placebo-controlled, double-blind phase II trial (TADAFER IIb) by Hiroaki Tanaka, Yasuhiro Tanaka, Yoichi Aoki, Masayuki Endo, Tomoaki Ikeda, Shintaro Maki, Masafumi Nii, Kayo Tanaka, Satoshi Tamaru, Toru Ogura, Yuki Nishimura, Tomomi Kotani, Akihiko Sekizawa, Masahiko Nakata, Koji Nakamura, Kenji Nagao, Ichiro Yasuhi, Hiroshi Kawamura, Sho Takakura, Mayumi Kotera, Takafumi Ushida, Norihiko Kikuchi, Tadatsugu Kinjo, Mayumi Takano, Sachie Suga, Michi Kasai, Osamu Yasui, Yuka Maegawa, Shigeru Aoki, Yoshio Yoshida

“…To establish further evidence for the efficacy of tadalafil in this setting, we planned a multicentre, randomised, placebo-controlled, double-blind trial.Methods and analysis This trial will be conducted in 180 fetuses with fetal growth restriction enrolled from medical centres in Japan; their mothers will be randomised into three groups: arm A, receiving two times per day placebo; arm B, receiving one time per day 20 mg tadalafil and one time per day placebo and arm C, receiving 20 mg two times per day tadalafil. …”
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Characteristics of the Volunteer Service Management System for National Parks in the United Kingdom by Tianyi REN, Chuanbo HONG, Yinglin WANG

“…This research aims to critically analyze and summarize the core characteristics of the UK’s volunteer service management system for national parks, so as to offer insights and references for China’s ongoing efforts in establishing its own volunteer service management system for national parks.MethodsThis research takes 14 national parks in the UK as the research object, collects the official data and literature on national parks in the UK and systematically sorts out special management contents such as management documents, management agencies, and management funds through text analysis. Then the research summarizes the characteristics of volunteer service management in each national park and combines similar characteristics, based on which the characteristics of the universal volunteer service management system for national parks in the UK are finally summarized. …”
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Pre-operative DNA methylation marks as predictors of weight loss outcomes after sleeve gastrectomy by Guillermo Paz-López, Teresa M. Linares-Pineda, Andrés González-Jiménez, Raquel Sancho-Marín, Luis Ocaña-Wilhelmi, Francisco J. Tinahones, Sonsoles Morcillo, Carolina Gutiérrez-Repiso

“…Of them, 59,308 DMPs were annotated to genes. KEGG enrichment analysis showed that pathways involved in the signalling of MAPK, Wnt, mTor, FoxO and AMPK, among others, were involved in weight loss trajectory.A stepwise logistic regression using the DMPs with an absolute Δβ >0.2 showed that higher methylation levels in the CpG sites cg02405213 (mapping to JAK2) (OR: 1.20098, [0.9586, 1.5044]) and cg01702330 (OR: 2.4426, [0.5761, 10.3567]), were shown to be associated with a higher probability of achieving >60 %EWL after sleeve gastrectomy, whereas higher methylation levels in the CpG site cg04863892 (mapping to HOXA5) were associated with lower probability of achieving >60 %EWL after sleeve gastrectomy (OR: 0.7966, [0.5637, 1.1259]). …”
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HIV self-testing alone or with additional interventions, including financial incentives, and linkage to care or prevention among male partners of antenatal care clinic attendees in... by Augustine T Choko, Elizabeth L Corbett, Nigel Stallard, Hendramoorthy Maheswaran, Aurelia Lepine, Cheryl C Johnson, Doreen Sakala, Thokozani Kalua, Moses Kumwenda, Richard Hayes, Katherine Fielding

“…The lottery arm was dropped at interim analysis. Overall, 46 male partners were confirmed to be HIV positive, 42 (91.3%) of whom initiated ART within 28 days; 222 tested HIV negative and were not already circumcised, of whom 135 (60.8%) were circumcised as part of the trial. …”
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Evolving concepts in intracranial pressure monitoring - from traditional monitoring to precision medicine by Rohan Mathur, Lin Cheng, Josiah Lim, Tej D. Azad, Peter Dziedzic, Eleanor Belkin, Ivanna Joseph, Bhagyashri Bhende, Sudha Yellapantula, Niteesh Potu, Austen Lefebvre, Vishank Shah, Susanne Muehlschlegel, Julian Bosel, Tamas Budavari, Jose I. Suarez

“…This paper provides historical and physiological context to the problem of elevated ICP, provides an overview of the challenges of the current paradigm of ICP management strategies, and discusses advances in ICP waveform analysis, emerging non-invasive ICP monitoring techniques, and applications of machine learning to create predictive algorithms.…”
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Impacto del seguimiento al alta de Enfermería en Atención Primaria sobre los reingresos hospitalarios tempranos. Estudio observacional longitudinal by Raquel Sánchez-Ruano, Pedro López-Paterna, César Alfonso García-Hernández, Ricardo Rodríguez-Barrientos, Elvira Herrero-Sanchox, Marcos Pascual-García, Milagros Rico-Blázquez

“…Sociodemographic, clinical and discharge follow-up variables were included from the electronic medical records of Primary and Hospital Care. A descriptive analysis of the sociodemographic and clinical characteristics of the study population was conducted. …”
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Estimation of yield and nitrogen use efficiencies in hybrid maize varieties through site specific nitrogen management based on leaf color chart (LCC) by Suhail Fayaz, Raihana Habib Kanth, Tauseef A. Bhat, Eajaz Ahmad Dar, Zahoor Ahmad Shah, Moneesa Bashir, Aijaz Nazir, Bilkees Jamsheed, Mohd. Salim Mir, Zahoor A. Dar, Shailja Sharma, Aabid Hussain Lone, Dawood Yousuf, Nadhir Al-Ansari, Mohamed A. Mattar, Ali Salem

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The Role of Potassium Phosphite in Enhancing the Activity of Defense Enzymes in Rice Infected by Sheath Blight by Milad Habibi Daronkolaei, Mohammad Ali Tajick Ghanbary, Valiollah Babaeizad, Heshmatollah Rahimian, Ali Dehestani

“…Results: The results of the analysis of variance showed that the effects of the treatments were significant in all sources of changes in the severity of the disease and the activity of PPO and SOD enzymes. …”
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How can cervical screening meet the needs of vulnerable women? A qualitative comparative study with stakeholder perspectives from seven European countries by Partha Basu, Martin McKee, Adriana Băban, Marc Bardou, Ana Fernandes, Margarida Teixeira, Rebecca Moore, Nuno Lunet, David Ritchie, Romeu Mendes, Eric Lucas, Paolo Giorgi Rossi, Rosa Legood, Berit Andersen, Diana Taut, Keitly Mensah, João Firmino-Machado, Mariana Amorim, Mette Tranberg, Laura Bonvicini, Anneli Uusküla, Pia Kirkegaard, Paola Mantellini, Ines Baia, Liu Sun, Lise Rochaix, Camilla Fiorina, Luca Ghirotto, Anna Tisler, Firmino Machado, Rikke Buus Bøje, Raquel Rico Berrocal, Noemi Auzzi, Kerli Reintamm, Yulia Panayotova, Tatyana Kotzeva, Anna Foss, Rachel Greenley, Letizia Bartolini, Giusy Iorio, Cláudia Gouvinhas, Florian Nicula, Alexandra Tolnai, Vanessa Moore, Isabel Mosquera Metcalfe, Violette Delisle, Irina Todorova, Helena Ros Comesana, Meritxel Mallafré-Larrosa, Ginevra Papi, Nicoleta-Monica Pașca, Christiane Dascher-Nadel, Olivra Djuric

“…The meetings were audio or video recorded, transcribed and translated into English for qualitative framework analysis.Results We engaged 120 participants in the Collaborative User Boards. …”
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CD21low B cells reveal a unique glycosylation pattern with hypersialylation and hyperfucosylation by Peter Tobias Felixberger, Peter Tobias Felixberger, Geoffroy Andrieux, Andrea Maul-Pavicic, Andrea Maul-Pavicic, Sigune Goldacker, Sigune Goldacker, Ina Harder, Ina Harder, Sylvia Gutenberger, Sylvia Gutenberger, Jonathan J. M. Landry, Vladimir Benes, Till Fabian Jakob, Melanie Boerries, Melanie Boerries, Lars Nitschke, Reinhard Edmund Voll, Reinhard Edmund Voll, Klaus Warnatz, Klaus Warnatz, Baerbel Keller, Baerbel Keller

“…This hypersialylation and hyperfucosylation were particularly induced by activation with anti-IgM and interferon-γ (IFN-γ). Transcriptome analysis suggested that naïve-like CD21low B cells possess a comprehensively reorganised glycosylation machinery, with anti-IgM/IFN-γ having the potential to initiate these changes in vitro.ConclusionCD21low B cells are hypersialylated and hyperfucosylated. …”
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Solid Predominant Histology and High Podoplanin Expression in Cancer-Associated Fibroblast Predict Primary Resistance to Osimertinib in EGFR-Mutated Lung Adenocarcinoma by Yuji Uehara, MD, Hiroki Izumi, MD, PhD, Tetsuro Taki, MD, PhD, Tetsuya Sakai, MD, PhD, Hibiki Udagawa, MD, PhD, Eri Sugiyama, MD, PhD, Shigeki Umemura, MD, PhD, Yoshitaka Zenke, MD, PhD, Shingo Matsumoto, MD, PhD, Kiyotaka Yoh, MD, PhD, Shoko Kubota, MD, PhD, Keiju Aokage, MD, PhD, Naoya Sakamoto, MD, PhD, Shingo Sakashita, MD, PhD, Motohiro Kojima, MD, PhD, Michiko Nagamine, MD, PhD, Yukio Hosomi, MD, PhD, Masahiro Tsuboi, MD, PhD, Koichi Goto, MD, PhD, Genichiro Ishii, MD, PhD

“…In the multivariable analysis, solid predominant histology and high-podoplanin expression in CAFs were independently associated with worse PFS. …”
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A phase 2, double-blind, placebo-controlled trial of a valproate/lithium combination in ALS patients by M.-C. Boll, M. Alcaraz-Zubeldia, C. Rios, D. González-Esquivel, S. Montes

“…Results: Out of 42 patients enrolled, 20 individuals receiving VPA-Li and 18 on placebo treatment were included in the final analysis. Forty-five percent of patients receiving VPA-Li completed the trial, whereas only 22.22% of patients in the placebo group attended the final visit 18 months later (P = 0.09). …”
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gga-miR-6634-5p Affects the proliferation and steroid hormone secretion of chicken (Gallus Gallus) granulosa cells by targeting MMP16 by Yixiang Tian, Xing Wu, Zihao Zhang, Yijie Li, Meng Hou, Ruirui Jiang, Donghua Li, Yanhua Zhang, Yulong Guo, Guoxi Li, Xiaojun Liu, Xiangtao Kang, Yadong Tian, Wenting Li, Yuetao Li

“…It was further found by functional enrichment analysis, qRT-PCR, western blot (WB) and ELISA that MMP16 may play a regulatory role in GCs proliferation as well as steroid hormone secretion through the mTOR signaling pathway and PPAR signaling pathway. …”
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Potential implications of thyroid autoantibodies in children, adolescents, and young adults with thyroid nodules in Japan: The Fukushima Health Management Survey by Rina Tazaki, Yurie Kobashi, Nana Nakahata, Mahiro Asano, Norikazu Abe, Haruka Ejiri, Ayako Sato, Natsuki Nagamine, Chisato Takahashi, Yukie Yamaya, Manabu Iwadate, Takashi Matsuzuka, Satoshi Suzuki, Tetsuya Ohira, Satoru Suzuki, Fumihiko Furuya, Hiroki Shimura, Shinichi Suzuki, Susumu Yokoya, Shunichi Yamashita, Hitoshi Ohto, Seiji Yasumura

“…A logistic regression analysis was performed using tab-positivity as an objective variable. …”
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A Series of Novel Alleles of <i>Ehd2</i> Modulating Heading and Salt Tolerance in Rice by Peng Xu, Shulei Hao, Xiaoxia Wen, Guifang Ma, Qinqin Yang, Ling Liu, Galal Bakr Anis, Yingxin Zhang, Lianping Sun, Xihong Shen, Qunen Liu, Daibo Chen, Yongbo Hong, Yuyu Chen, Xiaodeng Zhan, Shihua Cheng, Liyong Cao, Weixun Wu

“…Salinity stress significantly hampers rice growth and productivity. Transcriptomic analysis of <i>elh10</i> and ZH8015 seedlings exposed to salt stress for 24 h identified 5150 differentially expressed genes (DEGs) at the seedling stage, predominantly linked to stress response pathways. …”
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HPLC-MS standardization and validation methods for determination of calactin content in dichloromethane fraction of Calotropis gigantea (L.) Dryand. (Niu jiao gua) stem bark and it... by Naphat Kaewpaeng, Sudaporn Wongwan, Khemmachat Pansooksan, Chanakan Chailom, Pattaraporn Chaisupasakul, Dumrongsak Pekthong, Nitra Nuengchamnong, Zhi-Hong Jiang, Li-Ping Bai, Piyarat Srisawang, Supawadee Parhira

“…However, quantitative analysis of calactin content in CGD by using a high-performance liquid chromatography (HPLC) – ultraviolet (UV) method was limited. …”
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Ovarian stimulation alternatives for in vitro production of embryos in water buffaloes by Alexis Bandeo, José Luis Konrad, Natalia Vallejos, Pablo Ponce, Marina J. Sansinena, Gustavo Crudeli, Pablo Maldonado-Vargas

“…Descriptive statistics and analysis of variance were conducted on the obtained data, considering population and follicular size, quantity and quality of oocytes, and embryo production, with a significance level (α) of 5%. …”
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