Antibody Avidity and Neutralizing Response against SARS-CoV-2 Omicron Variant after Infection or Vaccination by Francesca Dapporto, Serena Marchi, Margherita Leonardi, Pietro Piu, Piero Lovreglio, Nicola Decaro, Nicola Buonvino, Angela Stufano, Eleonora Lorusso, Emilio Bombardieri, Antonella Ruello, Simonetta Viviani, Eleonora Molesti, Claudia Maria Trombetta, Alessandro Manenti, Emanuele Montomoli

“…Samples were collected from 42 SARS-CoV-2 infected patients during the first pandemic wave, 50 subjects who received 2 doses of mRNA vaccine before the Omicron wave, 44 subjects who received 3 doses of mRNA vaccine, and 35 subjects who received heterologous vaccination (2 doses of adenovirus-based vaccine plus mRNA vaccine) during the Omicron wave. …”
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Impact of COVID-19 pandemic measures on hospitalizations and epidemiological patterns of twelve respiratory pathogens in children with acute respiratory infections in southern Chin... by Wei Wang, Xiaojuan Luo, Zhenmin Ren, Xiaoying Fu, Yunsheng Chen, WenJian Wang, Yanmin Bao, Yuejie Zheng, Ke Cao, Jiehua Chen

“…Human Parainfluenza Virus, Human Metapneumovirus, Human Bocavirus (HBOV), and Human Coronavirus remained active during Phase 1, while Mycoplasma pneumoniae (Mp) and Adenovirus (ADV) were low, and Respiratory Syncytial Virus (RSV) lacked a seasonal peak in 2022. …”
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Analysis of respiratory pathogen detection in hospitalized children with acute respiratory tract infections after ending the zero COVID policy by Xuena Xu, Yizhu Zhang, Lina Xu, Wujun Jiang, Chuangli Hao

“…Nasopharyngeal aspirates (NPAs) were obtained from children with ARTIs (aged ≤ 16 years) at the Children’s Hospital of Soochow University for the detection of respiratory syncytial virus (RSV), influenza A (FluA), FluB, human parainfluenza virus (HPIV), adenovirus (ADV), human rhinovirus (HRV), bocavirus (BoV), human metapneumovirus (HMPV), and mycoplasma pneumoniae (MP). …”
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Correlation Between Inflammatory Markers and Pathogenic Bacteria in Children’s Winter Respiratory Infections in Xinjiang by Wang L, Wang W, Wang J, Zhu L, Luo J

“…Using Indirect Immunofluorescence Antibody test(IFA), blood routine, C-reactive Protein (CRP), and High-sensitivity C-reactive Protein(hsCRP), we detected nine respiratory pathogens (Respiratory syncytial virus; Influenza A virus; Influenza B virus; Parainfluenza virus; Adenovirus; Mycoplasma pneumoniae; Chlamydia pneumoniae; Legionella pneumophila type 1; Rickettsia Q) in all patients and analyzed their distribution and correlation. …”
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Respiratory pathogen spectrum and epidemiological trends in Hainan Province: a retrospective analysis from 2017 to 2023 by Lvying Wu, Jianan Zhang, Zhiming Wu, Hua Pei, You Zhang

“…A total of 21,036 patients with RTI of all age groups were enrolled and tested for nine common pathogens: influenza A virus (Flu-A), influenza B virus (Flu-B), parainfluenza virus (PIV), Adenovirus (AAV), respiratory syncytial virus (RSV), Q fever Rickettsiae (Q Fever), Legionella pneumophila (L. pne), Chlamydia pneumoniae (C. pne), and Mycoplasma pneumoniae (M. pne). …”
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Impact of COVID-19 pandemic restrictions and relaxation on pediatric respiratory virus hospitalizations in Southwest China: a retrospective study by Ting Gong, Fengqi He, Yongle Zhao, Shuya Lu, Daiyin Tian, Daiyin Tian

“…Data on respiratory syncytial virus (RSV), adenovirus (ADV), influenza A virus (IAV), influenza B virus (IBV), and parainfluenza virus (PIV)—were collected and analyzed across pre-pandemic, pandemic, and post-pandemic periods.ResultsA total of 59173 children were included. …”
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Leupaxin promotes hepatic gluconeogenesis and glucose metabolism by coactivation with hepatic nuclear factor 4α by Xiaomin Luo, Fang Liu, Lijun Zhu, Caizhi Liu, Ruhui Shen, Xiaoyin Ding, Yufan Wang, Xiaofang Tang, Yongde Peng, Zhijian Zhang

“…We further leveraged cell cultures and mouse models to validate the functional importance of molecular pathway during gluconeogenesis by using adenovirus-mediated overexpression and adeno-associated virus shRNA–mediated knockdown both in vivo and ex vivo, such as in ob/db/DIO mice, HepG2 and primary hepatocytes. …”
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Inhibition of S100A8/A9 ameliorates neuroinflammation by blocking NET formation following traumatic brain injury by Guihong Shi, Yiyao Cao, Jianye Xu, Bo Chen, Xu Zhang, Yanlin Zhu, Liang Liu, Xilei Liu, Luyuan Zhang, Yuan Zhou, Shenghui Li, Guili Yang, Xiao Liu, Fanglian Chen, Xin Chen, Jianning Zhang, Shu Zhang

“…Inhibition of S100A8/A9 effectively attenuated NET-mediated neuroinflammation; however, when PAD4 was overexpressed in the brain using adenovirus, leading to an increased formation of NET in the brain, the anti-inflammatory effects of S100A8/A9 inhibition were markedly diminished. …”
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Investigation of the Mechanism Underlying Calcium Dobesilate-Mediated Improvement of Endothelial Dysfunction and Inflammation Caused by High Glucose by Yijun Zhou, Chaojun Qi, Shu Li, Xinghua Shao, Zhaohui Ni

“…A pentraxin 3 (PTX3) small interfering RNA (siRNA) intervention experiment was performed in the cells. An adenovirus-encapsulated PTX3 siRNA intervention experiment was performed in db/db mice. …”
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αAsarone alleviates neuronal injury by facilitating autophagy via miR-499-5p/PDCD4/ATG5 signaling pathway in ischemia stroke by Yonghuan Yan, Linfang Wu, Lu Wang, Dandan Wang, Mengting Huang, Jinyong Peng, Jinyong Peng, Yingying Huang, Yingying Huang, Yingying Huang

“…However, its mechanism of action remains poorly understood.MethodsThe effects of αAsarone on autophagy were examined by WB, RT-qPCR, immunofluorescence colocalization, transmission electron microscope, and autophagic flux activity was measured by infecting HT22 cells with mRFP-GFP-LC3 adenovirus. And then, cells were transfected with both mimic-miR-499-5p and inhibit-miR-499-5p to investigate the role of miR-499-5p in regulating the effects of αAsarone on stroke. …”
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Beta 3 adrenoreceptors protect from hypertrophic remodelling through AMP‐activated protein kinase and autophagy by Emilie Dubois‐Deruy, Roselle Gelinas, Christophe Beauloye, Hrag Esfahani, Lauriane Y.M. Michel, Chantal Dessy, Luc Bertrand, Jean‐Luc Balligand

“…Methods and results In vitro hypertrophic responses to phenylephrine (PE) were analysed in neonatal rat ventricular myocytes (NRVM) infected with a recombinant adenovirus expressing the human β3‐AR (AdVhβ3). We confirmed results in mice with cardiomyocyte‐specific moderate expression of human β3‐AR (β3‐TG) and wild‐type (WT) littermates submitted to thoracic transverse aortic constriction (TAC) for 9 weeks. …”
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LncRNA LUCAT1 offers protection against human coronary artery endothelial cellular oxidative stress injury through modulating hsa-miR-6776-5p/LRRC25 axis and activating autophagy f... by Yanjun Wang, Xin Zhang, Mengmeng Ren, Shu He, Hengjie Bie, Mengyang Duan, Zhiyuan Chen, Qiaowei Jia, Boyu Chi, Xiongkang Gan, Chengcheng Li, Yahong Fu, Hanxiao Zhou, Sheng Zhang, Qian Zhang, Fenghui An, Xiumei Chen, Enzhi Jia

“…Methods (1) Autophagy-related proteins including LC3, p62, Beclin1, ATG5, and ATG7 were immunohistochemical stained in coronary specimens; (2) The levels and function of autophagy in the HCAEC oxidative stress model were evaluated using western blot (WB), transmission electron microscopy (TEM), and mRFP-GFP-LC3 adenovirus transfection experiments; (3) The competing endogenous RNA (ceRNA) network of lncRNA LUCAT1/hsa-miR-6776-5p/LRRC25 axis was constructed and validated; (4) The expression levels of above autophagy-related RNAs in peripheral blood mononuclear cells (PBMCs) were verified by qPCR, and their diagnostic performance was subsequently analyzed using receiver operating characteristic (ROC) analysis. …”
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Changes in infant respiratory pathogens pre-, during, and post-COVID-19 non-pharmacological interventions in Beijing by Tongying Han, Yajuan Wang, Di Zhang, Ying Li, Li Zhang, Jin Yan, Chi Li, Shengnan Yang, Litao Guo, Huijuan Yan

“…The specimens were tested for 14 respiratory pathogens, including influenza virus A (Flu A), influenza virus B, respiratory syncytial virus, parainfluenza virus (PIV), adenovirus (ADV), human metapneumovirus (HMPV), human bocavirus, human rhinovirus (HRV), coronavirus, Chlamydia trachomatis, Chlamydia pneumoniae (C.pn), Mycoplasma pneumoniae, Bordetella pertussis, and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). …”
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Single-cell atlas reveals multi-faced responses of losartan on tubular mitochondria in diabetic kidney disease by Zhen Zhu, Guangxin Luan, Song Wu, Yiyi Song, Shuang Shen, Kaiyue Wu, Shengnan Qian, Weiping Jia, Jun Yin, Tao Ren, Jianping Ye, Li Wei

“…Detection of autophagic flux in cells by transfecting Ad-mCherry-GFP-LC3B dual fluorescence labeled adenovirus. Various fluorescent probes and energy detector are used to detect mitochondrial apoptosis, ROS, and respiration of mitochondrion. …”
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甲基转移酶3对人脑血管平滑肌细胞基因表达及增殖和迁移功能的影响 Effects of Methyltransferase 3 on Gene Expression, Proliferation, and Migration Functions of Human Brain Vascular Smooth Muscle Cells... by 孟晨曦1，孙洪英2，张佳2，毛戬2，杨阳3，策乐木格3 (MENG Chenxi1, SUN Hongying2, ZHANG Jia2, MAO Jian2, YANG Yang3, Celemuge3 )

“…Methods The transfected HBVSMC were divided into three groups: the blank group (virus-free), the control group (empty vector), and the interference group (METTL3-shRNA) by using small interfering RNA (shRNA) interference technology and adenovirus packaging method. The mRNA expression levels of cerebral small vessel disease-related genes including zinc finger protein 395 (ZNF395), zinc finger protein 548 (ZNF548), DIS3-like exosome 3’-5’ exonuclease (DIS3L), interleukin enhancer binding factor 3 (ILF3) and G protein-coupled receptor 107 (GPR107) were detected by real-time reverse transcriptional PCR (RT-qPCR). …”
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