Automated Zebrafish Spine Scoring System Based on Instance Segmentation by Wen-Hsin Chen, Tien-Ying Kuo, Yu-Jen Wei, Cheng-Jung Ho, Ming-der Lin, Huan Chen, Wen-Ying Lin

“…In studying new medicines for osteoporosis, researchers use zebrafish as animal subjects to test drugs and observe the growth situation of their vertebrae in the spine to confirm the efficacy of new medicines. …”
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Valproate-Induced Model of Autism in Adult Zebrafish: A Systematic Review by Diletta Camussi, Maria Marchese, Ferdinando Nicoletti, Filippo Maria Santorelli, Asahi Ogi

Subjects: “…adult zebrafish…”
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In vivo monitoring of leukemia-niche interactions in a zebrafish xenograft model. by Anja Arner, Andreas Ettinger, Bradley Wayne Blaser, Bettina Schmid, Irmela Jeremias, Nadia Rostam, Vera Binder-Blaser

“…In the present study, we established an innovative larval zebrafish xenotransplantation model, that allows the analysis of leukemic cells (LCs) within an orthotopic niche using time-lapse microscopic and flow cytometric approaches. …”
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Phenogenetics of cortical granule dynamics during zebrafish oocyte-to-embryo transition by Priscila García-Castro, Isabella Giambó-Falian, Ingrid Carvacho, Ricardo Fuentes

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Decoding the molecular, cellular, and functional heterogeneity of zebrafish intracardiac nervous system by Andrea Pedroni, Elanur Yilmaz, Lisa Del Vecchio, Prabesh Bhattarai, Inés Talaya Vidal, Yu-Wen E. Dai, Konstantinos Koutsogiannis, Caghan Kizil, Konstantinos Ampatzis

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Swimming through asymmetry: zebrafish as a model for brain and behavior lateralization by Alessandra Gobbo, Andrea Messina, Giorgio Vallortigara

“…However, nowadays lateralization is known to occur among all vertebrates, from primates to fish. Fish, especially zebrafish (Danio rerio), have emerged as a crucial model for exploring the evolution and mechanisms of brain asymmetry. …”
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Methylene blue at recommended concentrations alters metabolism in early zebrafish development by Niepukolie Nipu, Lai Wei, Lauren Hamilton, Hyojin Lee, Jith Thomas, Jan A. Mennigen

“…The Organisation for Economic Co-operation and Development zebrafish embryo toxicity test guideline omits MB recommendations, leading to inconsistent MB use in zebrafish research. …”
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Toxicity evaluation of herbicides used in sugarcane crops to Zebrafish (Danio rerio) by Gabrielli Aparecida Sanches Tesolin, Marina Menezes Marson, Claudio Martín Jonsson, António José Arsénia Nogueira, Daniel Andrade de Siqueira Franco, Sydnei Dionísio Batista de Almeida, Marcus Barifouse Matallo, Monica Accaui Marcondes de Moura

“…From the data obtained, it is concluded that the mixture of Velpar K and Gesapax 500 is moderately toxic to zebrafish and that the endpoints evaluated were useful in determining its toxicity.…”
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Knockdown of Zebrafish Blood Vessel Epicardial Substance Results in Incomplete Retinal Lamination by Yu-Ching Wu, Ruei-Feng Chen, Chia-Yang Liu, Fung Rong Hu, Chang-Jen Huang, I-Jong Wang

“…In the current study, we generated a transgenic zebrafish Bves (zbves) promoter-EGFP zebrafish line to investigate the expression pattern of Bves in the retina and to study the role of zbves in retinal lamination. …”
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Protocol to computationally average confocal images of zebrafish lymphatic vessels to identify phenotypes by Hannah Arnold, Virginia Panara, Amin Allalou, Katarzyna Koltowska

“…Summary: Characterization of zebrafish mutants is routinely time consuming as morphological phenotypes can be subtle or variable, thus hampering robust quantification. …”
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Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle Responses by Changquan Wang, Zhenmin Zhong, Peng Sun, Hanbing Zhong, Hongzhe Li, Fangyi Chen

“…The zebrafish has become an established model organism for the study of hearing and balance systems in the past two decades. …”
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Spatiotemporal control of translation in live zebrafish embryos via photoprotected mRNAs by Florian P. Weissenboeck, Melissa Pieper, Helena Schepers, Sophie Hötte, Nils Klöcker, Sabine Hüwel, Andreas van Impel, Stefan Schulte-Merker, Andrea Rentmeister

“…We demonstrate that translation of FlashCap-eGFP-mRNA can be triggered in zebrafish embryos with spatiotemporal control. The injected FlashCap-mRNA is stable for hours and remains muted. …”
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Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses by Karim Abu Nahia, Cecilia Lanny Winata

“…Here, we present a protocol for preparing a single-cell suspension of the zebrafish embryonic whole heart, detailing steps for heart extraction, cell dissociation, quantification, and quality assessment. …”
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THE EFFECT OF DIFFERENT LEVELS OF VITAMIN E ON THE REPRODUCTIVE PERFORMANCE OF ZEBRAFISH (Danio rerio) by N.B.P Utomo, M. Zairin Junior, T.L. Yusuf, I. Mokoginta, M. Bintang

“… This experiment was conducted to determine the dietary Vitamin E requirement for reproduction of broodstock zebrafish Danio rerio.  Four isonitrogenous (39% crude protein) and isocaloric (3,260 kcal digestible energy/kg diet) practical diets, namely diets A, B, C, and D with different levels of Vitamin E were fed to zebrafish broodstock.  …”
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Background predation risk induces anxiety-like behaviour and predator neophobia in zebrafish by Himal Thapa, Arash Salahinejad, Adam L. Crane, Ahmad Ghobeishavi, Maud C. O. Ferrari

“…Although spatial and object neophobia in zebrafish have received previous attention, little is known about the role of background risk in inducing neophobia in zebrafish. …”
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L-theanine modulates tissue cortisol levels in zebrafish after single stress by Vinícius Luís da Silva, Mariana Spanamberg Mayer, Andriele dos Reis Severo, Andressa Leal Zambra, Joanna Moraes De Almeida, Laura Moura Sestari, Mariana Migliorini Parisi, Ana Cristina Vendrametto Varrone Giacomini, Murilo Sander De Abreu, Josiane Woutheres Bortolotto, Gabriela Bonfanti-Azzolin

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Marigold: a machine learning-based web app for zebrafish pose tracking by Gregory Teicher, R. Madison Riffe, Wayne Barnaby, Gabrielle Martin, Benjamin E. Clayton, Josef G. Trapani, Gerald B. Downes

Subjects: “…Zebrafish…”
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Dimethomorph induces heart and vascular developmental defects by disrupting thyroid hormone in zebrafish embryos by You Wei, Yunlong Meng, Kun Jia, Weijian Lu, Yushan Huang, Huiqiang Lu

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Knockout of <i>dhx38</i> Causes Inner Ear Developmental Defects in Zebrafish by Mengmeng Ren, Xiang Chen, Liyan Dai, Jiayi Tu, Hualei Hu, Xiaohan Sun, Jiong Luo, Pei Li, Yiyang Fu, Yuejie Zhu, Weiqiang Sun, Zhaohui Tang, Mugen Liu, Xiang Ren, Qunwei Lu

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Protocol for automated tracking and quantification of adult zebrafish anxiety behavior using ZebraTrack by Atanu Pramanik, Shubham Nema, Yogesh Bhargava, Anamika Bhargava

“…Here, we present a protocol using ZebraTrack, an open-source ImageJ-based approach for distraction-free swimming video acquisition and automated tracking of adult zebrafish. We detail steps for mathematical calculations on 2D coordinates to extract various behavioral endpoints for anxiety-behavioral evaluation.For complete details on the use and execution of this protocol, please refer to Nema et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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