Seroprevalence and Associated Risk Factors of Infectious Bovine Rhinotracheitis (IBR) and Animal Owners’ Knowledge, Attitude and Practice (KAP) Towards the Disease in Selected Dist... by Abdisa Mitiku Danu, Abriham Kebede Deresa, Chala Guyassa Dinagde

“…Accordingly, a total of 384 serum samples were collected from both crossbreed (70) and local breed (314) cattle from purposively selected districts of East Wollega zone of Western Ethiopia. Competitive enzyme‐linked immunosorbent assay (ELISA) was used for testing glycoprotein antibodies (anti‐gB) for bovine herpes virus‐1 (BoHV‐1) virus in collected serum, and the obtained data were analysed by multiple logistic regressions by using R software 3.62 version. …”
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NCAPD3-mediated ferroptosis of 2,3,5,4’-Tetrahydroxystilbene-2-O-β-D-glucoside inhibits proliferation in T47D cells by Jianfen Shen, Shuo Zhang, Shuo Zhang, Yan Song, Yan Song, Leiming Yang, Leiming Yang, Qi Huang, Qi Huang, Pengyu Wang, Pengyu Wang, Youzhi Zhang, Youzhi Zhang

“…And the hub genes (transcripts) were selected using the bioinformatics analysis, quantitative polymerase chain reaction (qPCR) and Western blotting (WB). Moreover, cell cycle and ferroptosis were assessed using flow cytometry analysis and WB respectively. …”
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The availability and distribution of health services and resources across different regions in Afghanistan by Nelly Hegazy, Sherif El Deeb, Marwa Rashad Salem, Jerome A. Shaguy, Ramesh Nassery Mohammed, Abdullah Khawari, Jamshed Tanoli, Alaa Abouzeid, Alaa Abouzeid

“…Furthermore, the study underscores the significant impact of financial constraints and equipment shortages on the functionality of healthcare facilities, particularly in the Northeastern and Western regions. This analysis emphasises the need for targeted resource allocation and infrastructure improvements to address inequities in access to essential healthcare services, particularly for underserved populations, thereby facilitating the achievement of equitable health outcomes in Afghanistan.…”
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Estimation of chlorophyll-a in uncrewed aircraft systems imagery using autonomous surface vessel data with machine learning algorithms and feature selection techniques by Mohammad Shakiul Islam, Padmanava Dash, Abduselam M. Nur, Hafez Ahmad, Rajendra M. Panda, Jessica S. Wolfe, Gray Turnage, Lee Hathcock, Gary D. Chesser, Jr, Robert J. Moorhead

“…Field data for Chl-a algorithm development were collected simultaneously using an Autonomous Surface Vessel (ASV), a hand-held radiometer, and a UAS in the Western Mississippi Sound (WMS). To ensure the algorithms were developed using accurate remote sensing reflectance data, the spectral response function of the UAS was applied to the radiometer measurements. …”
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Cinnamic acid alleviates endothelial dysfunction and oxidative stress by targeting PPARδ in obesity and diabetes by Yizhen Bai, Dechao Tan, Qiaowen Deng, Lingchao Miao, Yuehan Wang, Yan Zhou, Yifan Yang, Shengpeng Wang, Chi Teng Vong, Wai San Cheang

“…The beneficial effects of CA were comparable to the positive control group, PIO. Western blotting results indicated that CA treatment upregulated the expression of peroxisome proliferator-activated receptor delta (PPARδ), and activated nuclear factor erythroid 2-related factor 2 (Nrf2)/ heme oxygenase-1 (HO-1) and AMP-activated protein kinase (AMPK)/ protein kinase B (Akt)/ endothelial nitric oxide synthase (eNOS) signaling pathways in mouse aortas in vivo and ex vivo. …”
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iPSC-derived exosomes as amphotericin B carriers: a promising approach to combat cryptococcal meningitis by Jingyu Zhao, Jingyu Zhao, Wei Fang, Yangjie Gao, Jiquan Chen, Guizhen Wang, Julin Gu

“…Purity was confirmed by Western blotting for markers CD9, CD63, and TSG101. …”
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Hypothermia regulates mitophagy and apoptosis via PINK1/Parkin-VDAC 3 signaling pathway during oxygen-glucose deprivation/recovery injury by Luying Zhang, Song Yang, Hao Cui, Chenchen Hang, Xingsheng Wang, Le An, Zhenyu Shan, Zhen Liang, Rui Shao, Ziren Tang

“…Expression of mitophagy-related proteins (Cleaved PINK1, Parkin, SQSTM1/p62, Beclin-1, LC3B II/LC3B I), apoptosis-related proteins (Bcl-2, Cytochrome C, caspase-3, cleaved caspase3) and VDAC3 were assessed using western blot analysis and quantitative real-time PCR. …”
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Symptom patterns in patients newly sick listed for common mental disorders and associations with work-related and socioeconomic factors: a cross-sectional study in Swedish primary... by Cecilia Björkelund, Dominique Hange, Eva-Lisa Petersson, Irene Svenningsson, Nashmil Ariai, Lilian Wiegner

“…Objective The aim was to determine symptom patterns of depression, anxiety and stress-related mental disorders in newly sick listed due to common mental disorders in Swedish primary care patients and to examine associations with sick leave diagnosis, also in relation to socioeconomic, work-related and demographic factors.Design Cross-sectional study.Setting Primary care in western Sweden.Participants From a randomised controlled trial, patients aged 18–67, seeking primary care and on sick leave due to depression, anxiety and/or mental stress, in total 341 individuals, during 2018–2020.Primary outcome measures Patterns of depressive, anxiety and stress symptoms measured via self-assessment questionnaires (Montgomery Asberg Depression Rating Scale-Self (MADRS-S), General Anxiety Disorder Scale-7 (GAD-7), Karolinska Exhaustion Disorder Scale (KEDS)), sick leave diagnosis, perception of Work Ability Index and job strain via the job strain model.Results A combination of high levels of depressive and stress-related symptoms was more frequent than single symptom clusters among persons with common mental disorders (CMD) on sick leave: 7% of the patients had scores above cut-off for one of the instruments MADRS-S, GAD-7 and KEDS, 12% above cut-off for two and 80% had above cut-off for all three instruments. …”
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The validation and cross-cultural adaptation of the PainDETECT questionnaire in osteoarthritis-related pain by Xiaofeng Chang, Shuxin Yao, Jie Wei, Lei Shang, Chao Xu, Jianbing Ma

“…A cohort of 241 knee osteoarthritis (KOA) patients completed the PDQ-CV, Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), EuroQol-5 Dimensions-5 Levels (EQ-5D-5 L), and Central Sensitization Inventory Chinese Version (CSI-CV). …”
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Amantadine modulates novel macrophage phenotypes to enhance neural repair following spinal cord injury by Shijie Yang, Beibei Yu, Qing Zhang, Yongfeng Zhang, Longhui Fu, Bisheng Zhou, Haining Wu, Jianzhong Li, Shouping Gong

“…Subsequently, immunofluorescence staining, flow cytometry, and western blotting were performed to validate the identified effects of amantadine on macrophage differentiation and inflammation. …”
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Exploring the inhibitory effect and mechanism of isorhamnetin therapy on oral squamous cell carcinoma based on network pharmacology and molecular docking by YU Fangfang, ZHOU Jingjing, YANG Jie, QU Huijuan, HUI Guangyan

“…Colony formation assay and Transwell assay were used to identify the effect of Iso on the proliferation and invasion of Cal-27 cells. Western blot was used to analyze the regulatory effects of different concentrations of Iso on estrogen receptor-1 (ESR1), phosphoinositide-3-kinase regulatory subunit-1 (PIK3R1), Src tyrosine kinase (SRC), and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway proteins. …”
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Low-Concentration Oxygen/Ozone Treatment Attenuated Radiculitis and Mechanical Allodynia via PDE2A-cAMP/cGMP-NF-κB/p65 Signaling in Chronic Radiculitis Rats by Junnan Wang, Mingyi Wu, Xiaowen Lin, Yun Li, Zhijian Fu

“…The expression of the tumor necrosis factor- (TNF-) α, interleukin- (IL-) 1β, IL-6, cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP), phosphodiesterase 2A (PDE2A), and nuclear factor- (NF-) κB/p65 in spinal dorsal horns was measured by enzyme-linked immunosorbent assay, polymerase chain reaction, and western blot on day 7 after surgery. Results. Chronic radiculitis was established in rats. …”
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Evaluation of a new human immunodeficiency virus antigen and antibody test using light-initiated chemiluminescent assay by Yijun Li, Fangfang Jin, Yunhui Li, Yan Li, Yajie Wang, Ximing Yang

“…Screening-reactive results were confirmed by Western blotting and nucleic acid testing.ResultsTotal imprecision was within 2.49%–6.56%. …”
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SARS-CoV-2 infection and antibody seroprevalence in routine surveillance patients, healthcare workers and general population in Kita region, Mali: an observational study 2020–2021... by Ulla Ashorn, Per Ashorn, Nigel Klein, Samba O Sow, Camilla Ducker, Elaine Cloutman-Green, Dagmar Alber, Fadima Cheick Haidara, Juho Luoma, Laura Adubra, Henry Badji, Fatoumata Diallo, Rikhard Ihamuotila, Owen Martell, Uma U Onwuchekwa, Oumar Samaké, Awa Traore, Kevin Wilson, Yue-Mei Fan

“…Objective To estimate the degree of SARS-CoV-2 transmission among healthcare workers (HCWs) and general population in Kita region of Mali.Design Routine surveillance in 12 health facilities, HCWs serosurvey in five health facilities and community serosurvey in 16 villages in or near Kita town, Mali.Setting Kita region, western Mali; local health centres around the central (regional) referral health centre.Participants Patients in routine surveillance, HCWs in local health centres and community members of all ages in populations associated with study health centres.Main outcome measures Seropositivity of ELISA test detecting SARS-CoV-2-specific total antibodies and real-time RT-PCR confirmed SARS-CoV-2 infection.Results From 2392 routine surveillance samples, 68 (2.8%, 95% CI: 2.2% to 3.6%) tested positive for SARS-CoV-2 by RT-PCR. …”
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The Petri Dish-N2B27 Culture Condition Maintains RPE Phenotype by Inhibiting Cell Proliferation and mTOR Activation by Hui Lou, Chunpin Lian, Fanjun Shi, Liqun Chen, Sicheng Qian, Hui Wang, Xiaoyun Zhao, Xiaoyan Ji, Jingfa Zhang, Guoxu Xu

“…Morphological and biological characteristics were investigated using light microscopy, Q-PCR, and western blot. Results. The retina would curl inwardly during the growth medium incubation period, releasing RPE sheets in the medium. …”
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Evaluation of the Therapeutic Effect of Traditional Chinese Medicine on Osteoarthritis: A Systematic Review and Meta-Analysis by Lin Wang, Xiao-Fei Zhang, Xue Zhang, Dong-Yan Guo, Yu-Wei Duan, Zhi-Chao Wang, Li-Shan Pei, Han Ru, Jiang-Xue Cheng, Ya-Jun Shi, Jun-Bo Zou

“…In this meta-analysis, 68.18% of the literature correctly described the conditions for the generation of random assignment sequences, only 6.82% of the literature correctly mentioned the hidden details of allocation, and all studies mentioned randomly assigned participants. Compared with Western medicine, the total effective rate (TER) of OA treatment in TCM was significantly increased and the recurrence rate (RR) was significantly decreased (P<0.00001). …”
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How efficient are specialized public health services in China? A data envelopment analysis and geographically weighted regression approach by Qian Bai, Qian Bai, Lieyu Huang, Yan Guo, Xin Xu, Zhouyixin Zhang, Yuan Wang, Hao Chen, Ying Bian, Ying Bian

“…Besides, substantial geographic variations were observed, with the eastern region exhibiting greater levels of technical and pure technical efficiency. Contrarily, the western region appeared to perform better in terms of scale efficiency. …”
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The ground beneath our feet: A critical reflection on 135 years of landscape evolution models for southern Africa by Rivoningo Khosa, Vela Mbele, Kelly Kirsten, Robyn Pickering

“…We also note that the key narratives that have driven research on landscape evolution have been largely shaped by selected prominent Western-based scientists. As we mark the centenary of the Taung discovery, we look toward a new era of landscape evolution research: one characterised by technological advancements and more diverse, local teams that will produce more quantitative, nuanced models for southern Africa and create richer, more dynamic backdrops for our own human evolution. …”
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Transcriptome analysis reveals modulations in glycosylation profiles of the mucosal barrier and their potential interaction with gut microbiota in weaned piglets by Hao Cheng, Hao Li, Yujie Zhao, Kai Yang, Jing Wang, Bie Tan, Xiaokang Ma

“…The current study aims to investigate the potential interaction between glycosylation profiles of the Ningxiang breed (NX) and Western Duroc × Landrace × Yorkshire breed (DLY) weaned piglets, and their characteristic microbes, employing integrated analyses of transcriptomics and metagenomics. …”
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Comparison of Therapeutic Effects of Different Rehabilitation Methods on Patients Undergoing Total Knee Arthroplasty: A Network Meta‐Analysis of Randomized Controlled Trials by Zhaokai Jin, Yi Tang, Hua Huang, Lei Chen, Zhongyi Zhang, Tianyou Ma, Zhengming Wang, Hai Su, Haojing Zhou, Shuaijie Lv, Peijian Tong

“…And use the range of motion (ROM), visual analog scale (VAS), Western Ontario and McMaster Universities osteoarthritis index (WOMAC) as the outcome measure to evaluate the effectiveness of various interventions. …”
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