Platinum drugs upregulate CXCR4 and PD‐L1 expression via ROS‐dependent pathways, with implications for novel combined treatment in gastric cancer by Xiaoyu Kang, Lin Zhang, Shushang Liu, Fei Wang, Haiming Liu, Fenli Zhou, Fei Wu, Haohao Zhang, Daiming Fan, Yongzhan Nie, Zhangqian Chen

“…Immunohistochemistry, western blot, and RT‐qPCR were performed to determine the expression pattern of CXCR4 and PD‐L1 in GC. …”
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BRD4 Inhibitor GNE-987 Exerts Anticancer Effects by Targeting Super-Enhancer-Related Gene LYL1 in Acute Myeloid Leukemia by Xu Sang, Yongping Zhang, Fang Fang, Li Gao, Yanfang Tao, Xiaolu Li, Zimu Zhang, Jianwei Wang, Yuanyuan Tian, Zhiheng Li, Di Yao, Yumeng Wu, Xinran Chu, Kunlong Zhang, Li Ma, Lihui Lu, Yanling Chen, Juanjuan Yu, Ran Zhuo, Shuiyan Wu, Zhen Zhang, Jian Pan, Shaoyan Hu

“…After GNE-987 was added to AML cells, cell proliferation slowed down, the cycle was disturbed, and apoptosis increased. Western blotting was used to detect BRD2 (bromodomain containing 2), BRD3 (bromodomain containing 3), BRD4, and PARP (poly ADP-ribose polymerase) proteins. …”
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Burden of NASH related liver cancer from 1990 to 2021 at the global, regional, and national levels by Shuang Li, Shuang Li, Shuangjiang Li, Linjing Guan, Mingjuan Li, Jiahui Zhao, Min Wu, Qiuyun Li, Hui Li, Guoqing Ouyang, Guangdong Pan, Guangdong Pan

“…East Asia, South Asia, and Southeast Asia reported the highest absolute case numbers, while Western, Southern, and Eastern Sub-Saharan Africa exhibited the highest ASRs. …”
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Annual change in the distribution and landscape health of mangrove ecosystems in China from 2016 to 2023 with Sentinel imagery by Yuchao Sun, Mingzhen Ye, Bin Ai, Zhenlin Lai, Jun Zhao, Zhuokai Jian, Xinyan Qi

“…Notably, the centroid of China’s mangroves shifted 26.23 km northeast from the western part of Guangdong. To evaluate the landscape health of mangrove reserves, various landscape pattern metrics were synthesized into the Landscape Health Composite Index through the application of the entropy weight method. …”
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Size-resolved Compositional Analysis and Source Apportionment of Submicron Aerosol during Lockdown Period Using HR-ToF-AMS by Prodip Acharja, Akash Vispute, Prasanna Lonkar, Suresh W. Gosavi, Sreyashi Debnath, Narendra G. Dhangar, Kaushar Ali, Gaurav Govardhan, Sachin D. Ghude

“…The back trajectories, cluster analysis, and potential source contribution function (PSCF) showed the industrial belt located in the western part of the study location to be the potential source regions of NR-PM1. …”
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Language function improvement and cortical activity alteration using scalp acupuncture coupled with speech-language training in post-stroke aphasia: A randomised controlled study by Bingbing Lin, Jinglei Ni, Xiao Xiong, Lanlan Zhang, Jian Song, Mengxue Wang, Linsong Chai, Yunshi Huang, Jia Huang

“…The primary outcome measure was the Western Aphasia Battery (WAB) score, while the secondary outcome was the oxygenated haemoglobin (OxyHb) index, which represents cortical activity as measured by functional near-infrared spectroscopy (fNIRS). …”
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The Protective Effects of Annexin A1 in Acute Lung Injury Mediated by Nrf2 by Hui Huang, Yuqin Shi, Yuequan Zhou

“…Gene and protein expression levels were measured using RT‐PCR, ELISA, and western blot analysis. Results AnxA1 alleviated LPS‐induced ALI by protecting lung tissue from damage, reducing the lung wet/dry (W/D) weight ratio, and improving LPS‐induced impaired lung function. …”
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Dıscrete-tıme dynamıc water qualıty ındex model ın coastal water by H. Hapoğlu, Ş. Camcıoğlu, B. Özyurt, P. Yildirim, L. Balas

“…Measurements were done at three different stations near marina entrances in Bucak, Kaş, and Fethiye Bays located at the south western Mediterranean coast of Turkey. The computed water quality index values and the sampled indicators data defined in terms of the deviation variables were used to identify the proposed third-order transfer function parameters. …”
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Nutrient Profiling Models in Low- and Middle-Income Countries Considering Local Nutritional Challenges: A Systematic Review by Marie Tassy, Ries van Dijk, Alison L Eldridge, Tsz Ning Mak, Adam Drewnowski, Edith JM Feskens

“…Nutrient profiling models (NPMs), initially developed to help reduce the prevalence of diet-related chronic diseases in Western countries, could be one solution to promote nutrient-dense foods in LMICs. …”
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Detection of low frequency artemisinin resistance mutations, C469Y, P553L and A675V, and fixed antifolate resistance mutations in asymptomatic primary school children in Kenya by Victor Osoti, Kevin Wamae, Leonard Ndwiga, Paul M. Gichuki, Collins Okoyo, Stella Kepha, Kibor Keitany, Regina Kandie, Stephen Aricha, Rosebella Kiplagat, Charles Mwandawiro, Philip Bejon, Robert W. Snow, Lynette Isabella Ochola-Oyier

“…Conclusion The genomic surveillance of asymptomatic school children in Kenya provides an early warning signal of at least 1 of the 3 validated artemisinin resistance mutations circulating in all regions in Western Kenya sampled except Homa Bay and Kisii Counties. …”
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PD-L1 positive platelets mediate resistance to immune checkpoint inhibitors in patients with colorectal cancer by Jiacheng Li, Jia Liu, Shifeng Yang, Yu Xia, Qingzhe Meng, Biying Sun, Yansong Liu, Bin Zhao, Jiaqi Jin, Hui Xu, Lihong Wang, Pengxia Zhang, Zhuoxin Cheng

“…Methods The functional depletion effects of PD-L1 positive platelets on TME and immune cells were measured via western blotting, immunofluorescence staining, qRT-PCR, ELISpot and flow cytometry. …”
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Hsp70/Bmi1-FoxO1-SOD Signaling Pathway Contributes to the Protective Effect of Sound Conditioning against Acute Acoustic Trauma in a Rat Model by Guoxia Zhu, Yongxiang Wu, Yang Qiu, Keyong Tian, Wenjuan Mi, Xinqin Liu, Yuanyuan Chen, Jinwen Jia, Jiasheng Luo, Lianjun Lu, Jianhua Qiu

“…Data from the rat model were evaluated by western blot, immunofluorescence, and RT-PCR. The results revealed that SC upregulated Hsp70, Bmi1, FoxO1, SOD1, and SOD2 expression in spiral ganglion neurons (SGNs). …”
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Jianpi Yiqi Busui prescription alleviates myasthenia gravis by regulating Th17 through the TAK1/P38 MAPK/eIF-4E signaling pathway by Zhuming Chen, Jing Lu, Tianying Chang, Dongmei Zhang, Yibin Zhang, Miao Liu, Tong Wu, Peng Xv, Jian Wang

“…ELISA and RT-qPCR were used to measure acetylcholine receptor antibody (AChR-Ab) and Th17-related cytokines, including IL-17, IL-21, IL-23, TNF-α, TGF-β, IL-1β, and IL-6. Western blot and immunofluorescence staining were used to detect the expression levels of key proteins and their phosphorylated forms, such as transforming growth factor beta-activated kinase 1 (TAK1), P38 mitogen-activated protein kinase (P38 MAPK), and eukaryotic initiation factor 4E (eIF-4E). …”
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Molecular cloning and expression dynamics of Atrogin-1 in muscle tissue of Russian sturgeon (Acipenser gueldenstaedtii) under high salt stress by Yong-Juan Mo, Yi-Li Gao, Shi-Jjian Xu, Bin Wang, Jingping Wu, Peng Yu, Ming Li, Xiangtao Kang, Zhi-Qiang Han

“…Quantitative PCR (qPCR) and western blot analysis were employed to assess Atrogin-1 expression levels under high salt stress. …”
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Adipose-derived stem cells promote the recovery of intestinal barrier function by inhibiting the p38 MAPK signaling pathway by Mei Yang, Wangbin Xu, Chaofu Yue, Rong Li, Xian Huang, Yongjun Yan, Qinyong Yan, Shisheng Liu, Yuan Liu, Qiaolin Li

“…Apoptosis-related proteins (Bcl-2, Bax, Caspase-3 and Caspase-9), tight junction proteins (ZO-1, Occludin, E-cadherin, and Claudin-1) and p38 MAPK pathway-associated protein were detected by Western blotting. In addition, cell viability and apoptosis was determined by a CCK-8 kit and flow cytometry, respectively. …”
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GC-derived exosomal circMAN1A2 promotes cancer progression and suppresses T-cell antitumour immunity by inhibiting FBXW11-mediated SFPQ degradation by Yikai Shen, Jie Lin, Tianlu Jiang, Xusheng Shen, Ying Li, Yiwang Fu, Penghui Xu, Lang Fang, Zetian Chen, Hongxin Huang, Yiwen Xia, Zekuan Xu, Linjun Wang

“…The molecular mechanism of exosomal circMAN1A2 was investigated via mass spectrometry, immunoprecipitation, Western blot, and single-cell RNA-sequencing data analyses. …”
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Immunogenicity of recombinant fragment of orthopoxvirus p35 protein in mice by Ya. A. Khlusevich, A. L. Matveev, E. P. Goncharova, I. K. Baykov, N. V. Tikunova

“…It was shown by ELISA and Western-blot analysis that immunized mice sera contained IgG antibodies specific to recombinant protein р35Δ12. …”
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Oxidative damage to DNA, expression of Mt-1, and activation of repair mechanisms induced by vanadium trioxide in cultures of human lymphocytes by V.A. Alcántara-Mejía, A.A. Beltrán-Flores, R.A. Mateos-Nava, L. Álvarez-Barrera, I.U. Bahena-Ocampo, E. Santiago-Osorio, E. Bonilla-González, J.J. Rodríguez-Mercado

“…The expression levels of sensor proteins (ATM and ATR) involved in DNA damage were determined via Western blotting, and the mRNA expression levels of metallothionein 1 (Mt-1) and genes involved in DNA repair (OGG1, APE1, XPB, XPD, MRE11, RAD50, Ku70, and Ku80) were estimated via RT-PCR and qPCR. …”
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COVID-19 vaccine uptake in Zimbabwe and Sierra Leone: an application of Health Belief Model constructs by Simon Ssentongo, Alex Muhereza, Morie Mustapha, Ruth Gemi, Dennis Cherian, Rebecca Waugh, Benjamin Crookston, Cougar Hall, Mary Linehan, Hayden Borg, Joshua West

“…Abstract Introduction While African countries, in general, experienced a milder COVID-19 impact compared to Western nations, they faced challenges with vaccine uptake. …”
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Evidence-based cancer care: assessing guideline adherence of multidisciplinary tumor board recommendations for breast and colorectal cancer in a non-academic medical center by Carl-Stephan Leonhardt, Leopold Lanzenberger, Raphael Puehringer, Ulla Klaiber, Irene Hauser, Oliver Strobel, Gerald Prager, Martin Bodingbauer

“…Yet, most patients in Western countries receive cancer care at non-academic medical centers. …”
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