miR-210 in Exosomes Derived from Macrophages under High Glucose Promotes Mouse Diabetic Obesity Pathogenesis by Suppressing NDUFA4 Expression by Feng Tian, Ping Tang, Zhilian Sun, Ruifen Zhang, Danhua Zhu, Junying He, Jixing Liao, Qinghua Wan, Jie Shen

“…Exosomes from mouse macrophage RAW264.7 cells were characterized by electron microscopy, combined with biomarker expression by western blot. Expression of miR-210 was determined by quantitative RT-PCR. …”
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Growth Hormone Increases BDNF and mTOR Expression in Specific Brain Regions after Photothrombotic Stroke in Mice by Sonia Sanchez-Bezanilla, Daniel J. Beard, Rebecca J. Hood, N. David Åberg, Patricia Crock, Frederick R. Walker, Michael Nilsson, Jörgen Isgaard, Lin Kooi Ong

“…Protein levels of pro-BDNF, total-mTOR, phosphorylated-mTOR, total-p70S6K, and phosporylated-p70S6K within the peri-infarct area, hippocampus, and thalamus were evaluated by western blotting at 30 days poststroke. Results. r-hGH treatment significantly increased pro-BDNF in peri-infarct area, hippocampus, and thalamus (p<0.01). r-hGH treatment significantly increased expression levels of total-mTOR in the peri-infarct area and thalamus (p<0.05). r-hGH treatment significantly increased expression of total-p70S6K in the hippocampus (p<0.05). …”
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Early onset neonatal bloodstream infections in South African hospitals by Genevieve Theron, Adrie Bekker, Larisse Bolton, Andrew Whitelaw, Arnoldus Engelbrecht, Louisa Erasmus, Aaqilah Fataar, Chandre Geldenhuys, Marlize Kunneke, Dave Le Roux, Natasha O’Connell, Kessendri Reddy, Natasha Rhoda, Lloyd Tooke, Mark Wates, Thandi Wessels, Angela Dramowski

“…Methods We retrospectively analysed blood culture-confirmed EO-BSI episodes at nine neonatal units from three central and six peripheral hospitals in the Western Cape Province, South Africa between 1 January 2017 and 31 December 2018. …”
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IL-1β Pretreatment Improves the Efficacy of Mesenchymal Stem Cells on Acute Liver Failure by Enhancing CXCR4 Expression by He Nie, Fangmei An, Jie Mei, Cheng Yang, Qiang Zhan, Qinglin Zhang

“…The efficacy and homing ability of IL-1β-pretreated MSCs (Pre-MSCs) were examined in a rat ALF model and compared with that of MSCs and normal saline. Then, Western blot was performed to detect the c-Met and CXCR4 expression of MSCs and Pre-MSCs and followed by flow cytometry to detect the meaningful indicators. …”
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CIVILIZATIONAL AND INSTITUTIONAL ASPECTS OF NATIONAL SELF-IDENTIFICATION IN UKRAINE: PHILOSOPHICAL-ANTHROPOLOGICAL APPROACH by M. I. Boichenko, O. V. Yakovleva, V. V. Liakh

“…The authors found that the analysis of the cultural and anthropological principles of national self-identity reveals two main opposed concepts: the concept of "eastern" cultural and social self-identity of Ukraine, which correlates with the metaphor of the split between "East" and "West", and the concept of "western" projection of the European future of Ukraine, which correlates with the metaphor of the bridge between "East" and "West". …”
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Rotavirus Vaccination Protects Against Diabetes Mellitus Type 1 in Children in Developed Countries: A Systematic Review and Meta-Analysis by Chrysoula Kosmeri, Achilleas Klapas, Nikolas Evripidou, Evanthia Kantza, Anastasios Serbis, Ekaterini Siomou, Fani Ladomenou

“…<b>Conclusions</b>: This analysis suggests that rotavirus vaccination may reduce the risk of T1D in children from high-income Western countries. While these findings are promising, they may not be generalizable to settings outside similar advanced healthcare systems. …”
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Primary results from the Japanese Heart Failure and Sudden Cardiac Death Prevention Trial (HINODE) by Kazutaka Aonuma, Kenji Ando, Kengo Kusano, Toru Asai, Koichi Inoue, Yukihiro Inamura, Takanori Ikeda, Takeshi Mitsuhashi, Toyoaki Murohara, Nobuhiro Nishii, Akihiko Nogami, Wataru Shimizu, Caroline Beaudoint, Torri Simon, Torsten Kayser, Hussin Azlan, Ngarmukos Tachapong, Joseph Yat‐Sun Chan, Valentina Kutyifa, Yasushi Sakata, For the HINODE Investigators

“…Abstract Aims The HINODE study aimed to analyse rates of mortality, appropriately treated ventricular arrhythmias (VA), and heart failure in Japanese patients and compared with those in Western patients. Methods and results After treatment decisions following contemporary practice in Japan, patients were prospectively enrolled into four cohorts: (i) internal cardioverter‐defibrillator (ICD), (ii) cardiac resynchronization therapy (CRT) defibrillator (CRT‐D), (iii) standard medical therapy (‘non‐device’: ND), or (iv) pacing (indicated for CRT; received pacemaker or CRT pacing). …”
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Peroxisome Proliferator-Activated Receptor γ Regulates Lipid Metabolism in Sheep Trophoblast Cells through mTOR Pathway-Mediated Autophagy by Kexing Hao, Jing Wang, Hengbin Yu, Lei Chen, Weibin Zeng, Zhengrong Wang, Guangdong Hu

“…After 12 h, 24 h, and 48 h of drug treatment, the levels of autophagy-related proteins were detected by Western blot, the triglyceride content and MDA level of cells were detected by colorimetry, and the lipid droplets and lysosomes were localized by immunofluorescence. …”
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Insulin-Attenuated Inflammatory Response of Dendritic Cells in Diabetes by Regulating RAGE-PKCβ1-IRS1-NF-κB Signal Pathway: A Study on the Anti-Inflammatory Mechanism of Insulin in... by Liding Zhao, Ya Li, Qingbo Lv, Min Wang, Yi Luan, Jiale Song, Guosheng Fu, Junbo Ge, Yunzeng Zou, Wenbin Zhang

“…The concentrations of IFN-γ and TNF-α were calculated using ELISA kits, and the proteins were detected using western blot. Results. In CD11c+ DCs derived from the spleens with hyperglycemia, the expression of CD83 and CD86 in diabetic mice was significantly upregulated, coupled with a higher secretion level of cytokines and chemokines, and increased phosphorylation of NF-κB and IκB. …”
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Mechanical Stress-Induced IGF-1 Facilitates col-I and col-III Synthesis via the IGF-1R/AKT/mTORC1 Signaling Pathway by Bin Yan, Canjun Zeng, Yuhui Chen, Minjun Huang, Na Yao, Jie Zhang, Bo Yan, Jiajun Tang, Liang Wang, Zhongmin Zhang

“…Moreover, the activation of pIGF-1R, pAKT, and pS6 was examined by Western blot analysis. To further explore the underlying mechanism, an IGF-1 neutralizing antibody, NVP-AEW541, and rapamycin were used. …”
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Ionizing radiation-induced disruption of Rela-Bclaf1-spliceosome regulatory axis in primary spermatocytes causing spermatogenesis dysfunction by Hongjian Zhou, Zhipeng Xu, Chun Jiang, Qiuyue Wu, Chuanyue Zhang, Zhenyu Liu, Xiaoxue Zhang, Weiwei Li, Yujia Pang, Jing Zhang, Wenju Pan, Min Chen, Xinyi Xia

“…Histological examination, sperm concentration and motility analysis, Western blotting (WB), and reverse transcription quantitative PCR (RT-qPCR) were used to evaluate testicular injury. …”
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Therapeutic effects of Pueraria lobata (Willd.) Ohwi root and Hovenia dulcis Thunb. extracts on alcoholic liver disease: Network pharmacology and experimental validation by Zhendong Chen, Yu Yue, Hongyan An, Haisu Yan, Hyeok-Joo Park, Pei Lin

“…Biochemical assays and Western blot analyses were performed to assess the levels of oxidative stress markers, antioxidant enzymes, and inflammatory cytokines. …”
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Intrauterine Malnutrition Reduced Long Leptin Receptor Isoform Expression and Proinflammatory Cytokine Production in Male Rat Pulmonary Endothelial Cells Stimulated by Lipopolysacc... by Aleksandro M. Balbino, Marina M. Silva, Gabriela A. Azevedo, Noemi L. Gil, Renaide R. Ferreira, Leila A. dos Santos, Rebéca M. Gasparin, Liliam Fernandes, Maristella A. Landgraf, Richardt G. Landgraf

“…Six hours after stimulation, the production of inflammatory mediators (PGE2, LTB4, IL-1β, and IL-6) was evaluated using commercial ELISA kits, and Western blotting was performed to investigate p38MAPK, NF-κB, and ObRb expression. …”
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DKK3 promotes renal fibrosis by increasing MFF-mediated mitochondrial dysfunction in Wnt/β-catenin pathway-dependent manner by Jianling Song, Yanxia Chen, Yan Chen, Minzi Qiu, Wenliu Xiang, Ben Ke, Xiangdong Fang

“…CCK-8 and flow cytometry were utilized to assess cell viability and apoptosis. Western blotting, IHC, and qRT-PCR were performed to detect molecular expression levels. …”
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Nephropathy in Hypertensive Animals Is Linked to M2 Macrophages and Increased Expression of the YM1/Chi3l3 Protein by Paula Andréa Malveira Cavalcante, Natalia Alenina, Alexandre Budu, Leandro Ceotto Freitas-Lima, Thaís Alves-Silva, Juan Sebastian Henao Agudelo, Fatimunnisa Qadri, Niels Olsen Saraiva Camara, Michael Bader, Ronaldo Carvalho Araújo

“…We examined the kidneys of TGM123-FVB/N animals to assess macrophage polarization and end-organ damage. mRNA expression was evaluated using real-time PCR, and protein levels were assessed through ELISA, CBA, Western blot, and immunofluorescence. Histology confirmed high levels of renal collagen. …”
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Ascorbic Acid Reduces the Blood Boss After Total Knee Arthroplasty: Insights From a Randomized Controlled Trial by Pooya Hosseini-Monfared, MD, Alireza Mirahmadi, MD, Mohammad Mehdi Sarzaeem, MD, Soheil Pourshahryari, MD, Parisa Aminnia, MD, Mohammad Poursalehian, MD, Seyed Morteza Kazemi, MD

“…Patient-reported outcomes such as Oxford Knee Score, Western Ontario and McMaster Universities Osteoarthritis Index, Knee Injury and Osteoarthritis Outcome Score, and Forgotten Joint Score were used to evaluate the postoperative pain and function in the 6-month follow-up. …”
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Intramuscular Injection of Adenoassociated Virus Encoding Human Neurotrophic Factor 3 and Exercise Intervention Contribute to Reduce Spasms after Spinal Cord Injury by Yu-Xin Chang, Yan Zhao, Su Pan, Zhi-Ping Qi, Wei-Jian Kong, Yi-Ran Pan, Hong-Ru Li, Xiao-Yu Yang

“…We also measured the expression of KCC2 in the spinal cord by western blot. We found that AAV-NT3 gene therapy, exercise, and combination therapy all attenuated the frequency of spasms in the swimming test conducted at 6 weeks after spinal cord injury and increased rate-dependent depression of H-reflex. …”
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Seasonality of host-seeking Ixodes ricinus nymph abundance in relation to climate by Hoch, Thierry, Madouasse, Aurélien, Jacquot, Maude, Wongnak, Phrutsamon, Beugnet, Fréderic, Bournez, Laure, Cosson, Jean-François, Huard, Frédéric, Moutailler, Sara, Plantard, Olivier, Poux, Valérie, René-Martellet, Magalie, Vayssier-Taussat, Muriel, Verheyden, Hélène, Vourc’h, Gwenaël, Chalvet-Monfray, Karine, Agoulon, Albert

“…Seasonal patterns appeared different depending on the climate considered. Western temperate sites showed an early spring peak, a summer minimum and a moderate autumn and winter abundance. …”
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Comparative Evaluation and Measure of Accuracy of ELISAs, CLIAs, and ECLIAs for the Detection of HIV Infection among Blood Donors in China by Le Chang, Junpeng Zhao, Fei Guo, Huimin Ji, Lu Zhang, Xinyi Jiang, Lunan Wang

“…All plasma samples were tested by eight ELISA assays in 16 blood centers, followed by the detection of CLIA and ECLIA methods in the National Center for Clinical Laboratories (NCCL), further confirmed by nucleic acid testing (NAT) and Western blot (WB). Results. Of 1029 samples, 136 were confirmed as HIV positive. …”
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CircCCT2/miR-146a-5p/IRAK1 axis promotes the development of head and neck squamous cell carcinoma by Long He, Lanruo Li, Liting Zhao, Xiaoya Guan, Yujia Guo, Qi Han, Huina Guo, Hongliang Liu, Chunming Zhang

“…Dual-luciferase gene reporter, RIP, western blotting, and rescue experiments, were used to explore target-binding relationships and regulatory mechanisms. …”
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