Early Effects of Hyperbaric Oxygen on Inducible Nitric Oxide Synthase Activity/Expression in Lymphocytes of Type 1 Diabetes Patients: A Prospective Pilot Study by Ivana Resanovic, Zoran Gluvic, Bozidarka Zaric, Emina Sudar-Milovanovic, Aleksandra Jovanovic, Davorka Milacic, Radmilo Isakovic, Esma R. Isenovic

“…Expression of iNOS and phosphorylation of p65 subunit of nuclear factor-κB (NFκB-p65), extracellular-regulated kinases 1/2 (ERK1/2), and protein kinase B (Akt) were examined in lymphocyte lysates by Western blot. After exposure to HBOT, plasma CRP and FFA were significantly decreased (p<0.001). …”
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Assessing inter-basin groundwater input to the Verlorenvlei estuarine lake using stable isotopes and hydrochemistry by A. Welham, J. van Rooyen, A. Watson, J. Miller, R. Chow

“…Inter-basin aquifer contribution was investigated to assist in explaining Verlorenvlei's slow recovery since the recent 2015 – 2018 Western Cape drought. New insights: A proportion of groundwater from outside the topographic and surface-water delineated catchment supports Verlorenvlei during the dry month of April (i.e., G30F Langvlei sub-catchment). …”
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Anti-TNBC effects of Lappaol F by targeting epithelial-mesenchymal transition via regulation of GSK-3β/YAP/β-catenin and PI3K/AKT pathways by Qiqi Meng, Qiqi Meng, Zhiping Li, Xiaofeng He, Yuanhao Hu, Guiyun Wu, Jiawen Huang, Zhuohui Luo, Zhuohui Luo, Yingjie Hu, Xiaoling Shen

“…Flow cytometry, wound healing, transwell assay, western blot, RT-PCR, and immunofluorescence analysis were carried out to investigate the pharmacological mechanism of LAF against TNBC.ResultsLAF significantly inhibited the growth of MDA-MB-231 tumors, with downregulated tumor level of vimentin and upregulated level of ZO-1. …”
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Carbon Monoxide-Releasing Molecule-3 Suppresses Tumor Necrosis Factor-α- and Interleukin-1β-Induced Expression of Junctional Molecules on Human Gingival Fibroblasts via the Heme Ox... by Jia Lv, Yongsheng Liu, Shuhan Jia, Yuna Zhang, Haoyang Tian, Jingyuan Li, Hui Song

“…The protein and mRNA expression levels of adherens junction molecules (VE-cadherin and β-catenin) and tight junction molecules (zona occludens-1, ZO-1) were studied using western blot analysis and reverse transcription-quantitative polymerase chain reaction (RT-PCR). …”
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Conservation networks do not match the ecological requirements of amphibians by Matutini, Florence, Baudry, Jacques, Fortin, Marie-Josee, Pain, Guillaume, Pithon, Josephine

“…Here, we compared the ecological requirements of amphibian species with existing conservation network coverage in a human-dominated region of western France. First, we mapped suitable habitat distributions for nine species of amphibian with varying ecological requirements and mobility. …”
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Carvacrol acetate activated Nrf2 modulates mitophagy for the treatment of neurocyte oxidative stress induced by chlorpyrifos by Hong-Ling Zhou, Bei-Bei Wang, Xu-Li Fan, Xiao-Min Zhang, Ying Song

“…Furthermore, ROS and MDA levels were not significantly reduced, SOD enzyme activity did not increase, and mitochondrial membrane potential and mtDNA copy number did not improve. Western blot analysis showed that the expression of Tfam, Beclin1, and LC3II/LC3I proteins in the CAA group decreased significantly after Nrf2 inhibition. …”
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Antioxidant effect of Lactobacillus fermentum HFY02-fermented soy milk on D-galactose-induced aging mouse model by Tiantian Hu, Rui Chen, Yu Qian, Ke Ye, Xingyao Long, Kun-Young Park, Xin Zhao

“…The skin tissue structure of mice in the LF-HFY02 fermented soybean milk group was more complete, the collagen fibers were increased and arranged orderly and liver inflammation has improved compared with the model group. And Western blot analysis showed that LF-HFY02 effectively upregulated EGFR, SOD and GSH protein expression in mouse liver. …”
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miR-223 and Chromogranin A Affect Inflammatory Immune Cell Activation in Liver Metastasis of Neuroendocrine Neoplasms by Lukas Geisler, Katharina Detjen, Teresa Hellberg, Marlene Kohlhepp, Carsten Grötzinger, Jana Knorr, Ines Eichhorn, Raphael Mohr, Theresa Holtmann, Bertram Wiedenmann, Frank Tacke, Christoph Roderburg, Alexander Wree

“…Plasmin inhibitor a2-aP reverted those effects. Western Blot showed fragmented CgA following miR-223 knockdown, which altered the inflammatory potential of neutrophils. …”
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Mechanism and Evolution Control of Wide Coal Pillar Bursts in Multithick Key Strata by Wenhao Guo, Anye Cao, Chengchun Xue, Yang Hu, Songwei Wang, Qian Zhao

“…Coal mine pillar burst frequently occurs in Western China, which seriously restricts safe production. …”
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Novel Circulating Tumour Cell-Related Risk Model Indicates Prognosis and Immune Infiltration in Lung Adenocarcinoma by Lu Liang, Yueying Liu, Shiyao Jiang, Jingjing Huang, Hua He, Li Shen, Li Cong, Yiqun Jiang

“…Functional enrichment analysis was performed on differentially expressed genes (DEGs), followed by immune correlation analysis based on the results. In addition, western blot, CCK-8 and colony formation assays were used to validate the biological function of RAB26 in LUAD. …”
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A high offset stem design does not increase stem migration under full weight bearing in cementless total hip arthroplasty: a model-based RSA study by Tobias Reiner, Robert Sonntag, Jan Philippe Kretzer, Michael Clarius, Eike Jakubowitz, Stefan Weiss, Stefan Kinkel, Tilman Walker, Tobias Gotterbarm, Timo Albert Nees

“…Clinical outcomes were assessed using the modified Harris Hip Score (HHS) and the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC). …”
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Phenotypic diversity of bread wheat lines with introgressions from the diploid cereal Aegilops speltoides for technological properties of grain and f lour by L. V. Shchukina, I. F. Lapochkina, T. A. Pshenichnikova

“…Technological analyses were performed on grain obtained in Western Siberia and Moscow region. The aim of this study was to establish the possibilities of expanding the phenotypic diversity for technological properties of grain and flour as a result of such hybridization of bread wheat and the diploid cereal Aegilops speltoides. …”
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Inhibition of inflammatory cell infiltration by antagonistic peptides that specifically bind to CCR5 via regulation of the NF-kB signaling pathway in rats with colitis by Yang-Da Song, Yi-Hang Song, Si-Xue Liu, Hua-Rong Huang, Ying-Qiang Zhong

“…Histological evaluation, real-time PCR, western blotting and correlation analysis were performed to examine the effects of the CCR5 binding peptides on the infiltration of inflammatory cells and the NF-kB signaling pathway. …”
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Segmented evolution and exploration targets of the Cambrian platform margin in the Manxi area, Tarim Basin by Zongquan Hu, Fan Feng, Chengming Fang, Zicheng Cao, Tieyi Wang, Kangkang Guo, Yang Li, Shi Wang

“…Of the three major basins in western China, Tarim is the only basin with large-scale platform margin where no exploration breakthrough has been achieved yet. …”
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RIPK2-Mediated Autophagy and Negatively Regulated ROS-NLRP3 Inflammasome Signaling in GMCs Stimulated with High Glucose by Chenlin Gao, Jiao Chen, Fang Fan, Yang Long, Shi Tang, Chunxia Jiang, Jiying Wang, Youhua Xu, Yong Xu

“…RIPK2, LC3, caspase1, and IL-1β levels were measured by western blotting and RT-PCR. Autophagosomes were measured by GFP-RFP-LC3; ROS were detected by DCFH-DA. …”
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Assessment of the Relationship between Maxillary Posterior Teeth and Maxillary Sinus Using Cone-Beam Computed Tomography by Alaa Abdelqader Altaweel, Sami Mohammed Saad Sowairi, Ahmed Mohammed Saaduddin Sapri, Sama Abdulelah Saeedi, Asalah Hamad Alamri, Albtoul Ahmed Alnobi, Maha Fahad ALSharif, Ahmed Mohsen A Altokhi, Hisham Abbas

“…The aim of this study was to evaluate the relationship between maxillary posterior teeth and maxillary sinus floor in a population of the western area of Saudi Arabia, and if age, gender, and size may affect such distance. …”
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HSP60 inhibits DF-1 apoptosis through its mitochondrial signal peptide by Shengliang Cao, Yanlan Li, Lele Chen, Xiaojing Lei, Xiujuan Feng, Yubao Li

“…Then, the effect of knockdout of HSP60 on relevant apoptotic factors was assessed by Western blotting and RT-PCR analysis. The results showed that compared with the control DF-1 cells, HSP60 knockdout cells indicated significantly increased apoptosis rates, decreased Bax expression, and enhanced Caspase 3 expression. …”
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Enhanced membrane protein production in HEK293T cells via ATF4 gene knockout: A CRISPR-Cas9 mediated approach by Byung-Jo Choi, Ba Reum Kim, Ho Joong Choi, Ok-Hee Kim, Say-June Kim

“…Gene editing efficiency was compared between the two delivery systems, with the two-vector system demonstrating higher efficiency based on T7E1 assays. Western blot analysis confirmed ATF4 suppression and increased expression of membrane proteins, including E-cadherin and CD63. …”
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RETRACTED: The role of biofield energy treatment on psychological symptoms, mental health disorders, and stress‐related quality of life in adult subjects: A randomized controlled c... by Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Sambhu Mondal, Snehasis Jana

“…Abstract Background Western and Eastern cultures have practiced biofield energy therapy for thousands of years, but empirical research on effectiveness of this therapy is relatively nascent. …”
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Anti-PL-7/PL-12 antisynthetase syndrome associated with interstitial lung disease following SARS-COV-2 infection and vaccination: A case study review by Laura García-Bravo, Ángela Villegas, Bárbara López Uceda, Anaís Mariscal, Cristina Vadillo, M Asunción Nieto Barbero, Juan Luis Rodríguez-Hermosa, Beatriz Mediero Valeros, José Carlos Plaza-Hernández, Miguel Fernández-Arquero, María Guzmán-Fulgencio, Gloria Candelas-Rodríguez, Silvia Sánchez-Ramón, Juliana Ochoa-Grullón

“…Anti-ARS autoantibodies were identified using three complementary methods: immunoblotting, western blotting (WB) and the method considered the gold standard, immunoprecipitation (IP), which ensures accurate interpretation of results. …”
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