Dynamic changes in soil erosion and challenges to grain productivity in the black soil region of Northeast China by Jiawang Zhang, Ming Wang, Kai Liu, Zhan’ao Zhao

“…Wind erosion was concentrated in the western of Liao River Plain, the average erosion modulus is 0.81 t. hm−2. a-1. …”
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Notch Signaling Pathway Is Involved in bFGF-Induced Corneal Lymphangiogenesis and Hemangiogenesis by Fang Xie, Xue Zhang, Wenting Luo, Hongyan Ge, Dawei Sun, Ping Liu

“…Vascular endothelial growth factor-A (VEGF-A), Delta-like ligand 4 (Dll4), and Notch1 mRNA and protein expression were examined on days 1, 3, 7, and 14 by real-time polymerase chain reaction and western blot. Corneal cells were treated with ranibizumab, dexamethasone, and γ-secretase inhibitor (GSI). …”
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Oncogene OSTM1 Promotes Gastric-Cancer Metastasis by Modulating the Metastatic Microenvironment Through Altered Tumor-Cell Autocrine Signaling by Yucheng Tang, Yi Guo, Jiangyi Feng, Ziwei Wang

“…OSTM1 overexpression and knockdown cell lines were established to assess its effects on cancer-cell behavior through in vitro and in vivo experiments. Western blot and RT-qPCR were used to examine OSTM1’s regulation of S100A4 expression. …”
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Metallothionein 1B attenuates inflammation and hepatic steatosis in MASH by inhibiting the AKT/PI3K pathway by Canghai Guan, Xinlei Zou, Wujiang Shi, Jianjun Gao, Chengru Yang, Yifei Ge, Zhaoqiang Xu, Shaowu Bi, Xiangyu Zhong

“…This study investigates the role and potential mechanisms of metallothionein 1B (MT1B) in MASH through bioinformatics analysis and experimental validation. quantitative reverse transcription PCR and Western blot analyses confirm that MT1B expression is significantly downregulated in liver tissues of MASH patients, in high-fat diet–induced mouse models, and in hepatocytes induced by FFAs. …”
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Lysophosphatidic Acid Induced Apoptosis, DNA Damage, and Oxidative Stress in Spinal Cord Neurons by Upregulating LPA4/LPA6 Receptors by Yifan Yang, Jing Xu, Qingxin Su, Yiran Wu, Qizheng Li, Zongren Ma, Tao Ding

“…After clinical detection of LPA secretion, spinal cord neurons were treated with LPA (18 : 1); cell viability was analyzed by MTT assay, and LDH leakage was detected by LDH kit; cell apoptosis was detected by flow cytometry; ROS production was measured by DCFDA staining and MitoSOX Red Staining; the activation of the Gα12/Gα13 signaling pathway was detected by serum response factor response element (SRF-RE) luciferase reporter gene; the relationship among LPA, LPA4/6, and ROCK was examined by western blotting. In spinal cord neurons treated with LPA (18 : 1), cellular activity decreased and LDH release increased. …”
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Astrocytic Acid-Sensing Ion Channel 1a Contributes to the Development of Epileptic Cognitive Impairment by Wen Li, Huimin Zhou, Xiaona Li, Gengyao Hu, Dong Wei

“…Behavioral assessments were conducted using the Morris water maze (MWM). Western blotting and immunofluorescence were applied to evaluate ASIC1a and <i>Gfap</i> expression while analyzing intracellular calcium and extracellular glutamate (Glu) concentrations in primary cultured astrocytes isolated from the brains of 1 to 3-day-old mice and treated LPS. …”
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South Atlantic subtropical anticyclone responses to stratospheric aerosol injection by Thales Chile Baldoni, Michelle Simões Reboita, Natália Machado Crespo, João Gabriel Martins Ribeiro, Rosmeri Porfírio da Rocha

“…The South Atlantic Subtropical Anticyclone (SASA) is a key component of large-scale atmospheric circulation and is responsible for driving the climate in eastern Brazil and western Africa. Climate projections under warming scenarios suggest a strengthening, as well as a westward and southward expansion of this system. …”
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Soluble Receptor for Advanced Glycation End Products: A Protective Molecule against Intramyocardial Lipid Accumulation in Obese Zucker Rats? by Elena Dozio, Elena Vianello, Francesco Bandera, Erika Longhi, Stefano Brizzola, Manuela Nebuloni, Massimiliano M. Corsi Romanelli

“…Heart RAGE expression was explored by real-time RT-PCR and Western blot analyses. Serum levels of sRAGE (total and endogenous secretory form (esRAGE)) were quantified by ELISA. …”
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Methodological advances in seismic noise imaging of the Alpine area by Paul, Anne, Pedersen, Helle A., Bodin, Thomas, Kästle, Emanuel, Soergel, Dorian, Alder, Chloé, Lu, Yang, Nouibat, Ahmed

“…Our findings include detailed mapping of strong and abrupt Moho depth changes under the Western Alps, contrasting orientations of fast velocity directions between the upper and lower Alpine crust, and the absence of significant radial anisotropy everywhere in the European crust and shallow upper mantle, except in the Apenninic lower crust. …”
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Newborn screening for congenital hypothyroidism: worldwide coverage 50 years after its start by Marta Arrigoni, Nitash Zwaveling-Soonawala, Stephen H LaFranchi, A S Paul van Trotsenburg, Christiaan F Mooij

“…Recently, some countries of Central and Western Asia have implemented NBS programs for CH, and coverage has increased in several Asian countries. …”
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Characterization of rectus femoris lesions in knee osteoarthritis at different stages and the effect of ultrasound-guided acupotomy by Wen-Ying Yu, Wen-Ying Yu, Jing Liu, Jing Liu, Ze-Hao Lin, Ze-Hao Lin, Hong Liu, Hong Liu, Liang-Zhi Zhang, Liang-Zhi Zhang, Xiang-Long Feng, Xiang-Long Feng, Zhong-Biao Xiu, Zhong-Biao Xiu, Zhong-Biao Xiu

“…Additionally, ultrasound-guided acupotomy operations were performed on the rabbit model of late-stage KOA, and alterations in the key molecular markers of rectus femoris fibrosis were determined using Western Blot and qPCR methods.ResultsAs the disease progressed, the elastic modulus of the rectus femoris muscle in KOA rabbits gradually increased, the cross-sectional area gradually decreased, and the degree of fibrosis increased. …”
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DEEP STRUCTURE, TECTONIC EVOLUTION AND HYDROCARBON POTENTIAL OF THE KALAMIT SWELL (NORTHWESTERN SHELF OF THE BLACK SEA) ACCORDING TO DENSITY MODELING DATA by M.V. Kozlenko, Yu.V. Kozlenko

“…The southern one belongs to the subcontinental type of transitional block between the SP and the Western Black Sea Basin. The boundaries of the КS are determined, which are mostly controlled by faults. …”
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Effects of Ginsenoside Rg1 Regulating Wnt/β-Catenin Signaling on Neural Stem Cells to Delay Brain Senescence by Yue Xiang, Shun-he Wang, Lu Wang, Zi-ling Wang, Hui Yao, Lin-bo Chen, Ya-ping Wang

“…The following indicators were detected: (1) NSC culture identification, (2) the effect of LiCl on the proliferation and survival rate of NSCs, (3) the effect of ginsenoside Rg1 on the proliferation and survival of NSCs, (4) the growth of NSCs in each group observed by an optical microscope, (5) the cell cycle of each group detected by flow cytometry, (6) the proliferative ability of each group detected by BrdU, (7) the fluorescence intensity of Nestin and Sox2 of NSCs in each group observed by a fluorescence microscope, (8) the positive rate of senescence staining analyzed by SA-β-Gal staining, (9) the localization of β-catenin in NSCs observed by laser confocal microscopy, and (10) the changes of the Wnt/β-catenin pathway-related proteins in each group detected by Western blotting. Results. LiCl activates the Wnt/β-catenin pathway and promotes mouse neural stem cell senescence. …”
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A novel de novo GABRA2 gene missense variant causing developmental epileptic encephalopathy in a Chinese patient by Li Yang, Xingyu Wan, Ran Hua, Junhong Jiang, Baotian Wang, Rui Tao, De Wu

“…A mutant plasmid was constructed and transfected into 293 T cells. Western blotting (WB) was used to evaluate mutant protein expression, while co‐immunoprecipitation (Co‐IP) analyzed interactions with GABRB3 and GABRG2 proteins. …”
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Influence of the Multifunctional Biological Product Phytop 26.82 on the Growth and Development of Seed Potatoes by Vladislava Maslennikova, Vera Tsvetkova, Andrey Petrov, Rinat Galeev, Maxim Shulga, Natalia Gavrilets, Sergey Ryumkin, Anastasia Shulga, Evgeniy Ryadskiy, Kristina Koloshina, Inga Ryumkina

“…The study aimed to test a multifunctional technology for potatoes’ biological protection using Phytop 26.82 against black scurf (Rhizoctonia solani Kuhn) and Colorado potato beetle in Western Siberia. The experiment was conducted with the medium-maturing variety “Kemerovchanin.” …”
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Erythropoietin Activates Autophagy to Regulate Apoptosis and Angiogenesis of Periodontal Ligament Stem Cells via the Akt/ERK1/2/BAD Signaling Pathway under Inflammatory Microenviro... by Denghao Huang, Jie Lei, Xingrui Li, Zhonghao Jiang, Maoxuan Luo, Yao Xiao

“…Quantitative real-time polymerase chain reaction was conducted to examine mRNA expression level. Western blot and immunofluorescence were applied to testify the protein expression level. …”
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Injury mortality in South Africa: a 2009 and 2017 comparison to track progress to meeting sustainable development goal targets by Megan Prinsloo, Shibe Mhlongo, Rifqah A. Roomaney, Lea Marineau, Thakadu A. Mamashela, Bianca Dekel, Debbie Bradshaw, Lorna J. Martin, Carl Lombard, Rachel Jewkes, Naeemah Abrahams, Richard Matzopoulos

“…Post-mortem files for non-natural deaths were reviewed, with additional data from the Western Cape. Age-standardised rates, 95% confidence intervals (CIs), and incidence rate ratios (IRRs) were calculated for manner of death rate comparisons and for age groups. …”
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Expression of palmitoyl transferases in brain structures of mice genetically predisposed to depressive-like behavior by E. M. Kondaurova, T. V. Ilchibaeva, A. S. Tsybko, Е. G. Ponimaskin, V. S. Naumenko

“…Several brain region-specific features were detected in the immunodetection of palmitoyl transferase proteins. Western blot of the ZDHHC5 protein in the midbrain revealed two bands at 75 kDa and 55 kDa. …”
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MicroRNA-541-3p/Rac2 signaling bridges radiation-induced lung injury and repair by Jiandong Zhang, Lei Ma, Limin He, Quanxiao Xu, Yan Ding, Lidong Wang

“…Flow cytometry, polymerase chain reaction, and western blotting were used to analyze cell necroptosis, target gene expression, and target protein expression, respectively. …”
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miR-149 Alleviates Ox-LDL-Induced Endothelial Cell Injury by Promoting Autophagy through Akt/mTOR Pathway by Zhongsheng Zhu, Jinyu Li, Rui Tong, Xiaorong Zhang, Bo Yu

“…Autophagy was detected by immunofluorescence. RT-qPCR and western blot were carried out to determine the mRNA and protein expressions of Akt and mTOR. …”
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