Chemical Changes Under Heat Stress and Identification of Dendrillolactone, a New Diterpene Derivative with a Rare Rearranged Spongiane Skeleton from the Antarctic Marine Sponge <i>... by Andrea Prófumo, Conxita Avila, Adele Cutignano

“…The waters around the western Antarctic Peninsula are experiencing fast warming due to global change, being among the most affected regions on the planet. …”
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Teachers’ responses to racism and racist bullying in Dutch primary schools by Karen M. A. Sieben-Aduful, Roy A. Willems, Trijntje Völlink, Nico van der Wiel, Maria Sapouna, Pieter de Bruijn, Gemma Blok, Gemma Blok, Arjan E. R. Bos, Arjan E. R. Bos, Giel van Lankveld

“…BackgroundEarly in life, children with a non-White skin color, or a non-Western cultural or religious background, are susceptible of experiencing acts of racism. …”
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Acrylamide exposure promotes the progression of depression-like behavior in mice with CUMS via GSDMD-mediated pyroptosis by JianFeng Liu, Chenyang Han, Jian Shen, Yingcong Lin, Heping Shen, Genghuan Wang

“…Enzyme-linked immunosorbent assay (ELISA) was used to detect tissue inflammatory factor levels, hematoxylin and eosin (H&E) and Nissl staining to detect neuronal damage, immunohistochemical staining to detect IBA-1 expression, and Western-blotting to detect protein levels. GSDMD knockout (KO) mice and the GSDMD inhibitor LDC7559 were used to inhibit GSDMD. …”
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Role of the Btk-PLCγ2 Signaling Pathway in the Bone Destruction of Apical Periodontitis by Lina Wang, Hong Zhang, Ming Dong, Meina Zuo, Shuo Liu, Ying Lu, Weidong Niu

“…By silencing Btk, the expression of Btk, PLCγ2, and NFATc-1 was detected by real-time qPCR and Western blot, and osteoclastogenesis was detected by enzyme histochemical staining to further confirm the role of Btk in bone resorption. …”
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Ethanol extract of Polygonatum cyrtonema Hua mitigates non-alcoholic steatohepatitis in mice by Dongliang Chen, Yue Shen, Yue Shen, Fang Huang, Bo Huang, Shangfu Xu, Shangfu Xu, Lisheng Li, Lisheng Li, Jie Liu, Zheng Li, Xia Li

“…One hand, liver injury was assessed by serum enzymes, biochemistry, and histopathology; On the other hand, RNA-seq, qPCR, and Western blot were employed to investigate the related molecular mechanisms.Results211 metabolites were identified through UHPLC-MS/MS analysis. …”
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Tumor Necrosis Factor (TNF) Receptor Expression Determines Keratinocyte Fate upon Stimulation with TNF-Like Weak Inducer of Apoptosis by Xuening Wang, Dan Cheng, Guanglei Hu, Lili Liang, Fei Tan, Tong Xiao, Shengxiang Xiao, Yumin Xia

“…Through immunoprecipitation and Western blotting of keratinocyte lysates, we elucidated the associations between Fn14, TNFR-associated factor 2 (TRAF2), cellular inhibitor of apoptosis protein 1 (cIAP1), and TNFR1/2 molecules. …”
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GsMTx-4 venom toxin antagonizes biophysical modulation of metastatic traits in human osteosarcoma cells by Arianna Buglione, Giulia Alloisio, Chiara Ciaccio, David Becerril Rodriguez, Simone Dogali, Marco Luce, Stefano Marini, Antonio Cricenti, Magda Gioia

“…To elucidate the molecular mechanisms that translate biomechanical alterations into biochemical responses, we employed Western blotting, real-time quantitative RT-PCR, reactive oxygen species ROS assay, and the mechanosensitive channel blocker Grammostola MechanoToxin4 (GsMTx-4). …”
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The Protective Effects of Maresin 1 in the OVA-Induced Asthma Mouse Model by Guochun Ou, Qin Liu, Chengxiu Yu, Xiaoju Chen, Wenbo Zhang, Yong Chen, Tao Wang, Yongqiang Luo, Guolu Jiang, Mingmei Zhu, Hongmei Li, Mei Zeng

“…Mice were sacrificed 24 hours after the end of the last challenge, and serum, bronchoalveolar lavage fluid (BALF), and lung tissue were collected for further analysis. Western blotting was used to measure the protein level of IκBα, the activation of the NF-κB signaling pathway, and the expression of NF-κB downstream inflammatory cytokines. …”
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Association of tumour mutation burden with prognosis and its clinical significance in stage III gastric cancer by Ya-Lin Han, Li Chen, Xu-Ning Wang, Mao-Lin Xu, Rui Qin, Fang-Ming Gong, Peng Sun, Hong-Yi Liu, Zhi-Peng Teng, Zhao-Xia Li, Guang-Hai Dai

“…In the TCGA cohort, gene set enrichment analysis was performed, and the relationship between TMB, prognosis and clinicopathologic factors was analysed. Western blot and quantitative real-time polymerase chain reaction were used to detect the expression levels of both proteins and genes. …”
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Upregulation of Tumor Necrosis Factor-α-Induced Protein 8-Like 2 mRNA Is Negatively Correlated with Serum Concentrations of Tumor Necrosis Factor-α and Interleukin 6 in Type 2 Diab... by Yongliang Liu, Xinmei Wang, Yan Zhao, Peiqing Zhao, Lianqing Wang, Qiaoli Zhai, Xiaowei Zhang, Wenxiu Tian, Xinxin Xiang, Tao Li

“…TIPE2 expression was measured by Western blotting and real-time polymerase chain reaction (RT-PCR) in peripheral blood mononuclear cells (PBMCs) isolated from T2DM patients and healthy controls, and tumor necrosis factor-α (TNF-α), high-sensitivity C-reactive protein (hsCRP), interleukin 6 (IL-6), and other related biometabolic parameters were detected using a nephelometer or by ELISA. …”
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Liver Expression of Sulphotransferase 2A1 Enzyme Is Impaired in Patients with Primary Sclerosing Cholangitis: Lack of the Response to Enhanced Expression of PXR by Ewa Wunsch, Marta Klak, Urszula Wasik, Malgorzata Milkiewicz, Malgorzata Blatkiewicz, Elzbieta Urasinska, Olivier Barbier, Dariusz Bielicki, Dimitrios P. Bogdanos, Elwyn Elias, Piotr Milkiewicz

“…We studied the PXR/SULT2A1 axis in chronic cholestatic conditions: primary sclerosing cholangitis (PSC) and primary biliary cirrhosis (PBC). Materials/Methods. Western-blot/PCRs for SULT2A1/PXR were performed in PSC (n=11), PBC (n=19), and control liver tissues (n=19). …”
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Effects of Dendrobium Officinale Polysaccharides on Brain Inflammation of Epileptic Rats by Lin Zhang, Hua Peng, Jin Xu, Yixin Xu, You Yin, Bin He, Jianhua Zhuang

“…RT-PCR was used to measure the mRNA expression of IL-1β and TNF-α in the hippocampi of all groups; western blot was used to measure the protein expression of IL-1β and TNF-α and phosphorylation of ERK1/2, JNK, p38, and MKP-1 in the hippocampi of all groups at weeks 1, 2, 3, and 4 after modeling. …”
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Soil fertility differences due to tillage methods modulate maize yield formation at different planting densities by Li-Qing Wang, Da-Ling Ma, Hong-yue Liu, Shu-Ping Hu, Xiao-Fang Yu, Ju-Lin Gao

“…Abstract To address the problems of planting density and low soil nutrient content in maize cultivation and production in western Inner Mongolia. This study aims to elucidate the regulatory mechanism by which soil fertility augmentation affects maize yield formation under a variety of planting densities. …”
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RELATIONSHIP BETWEEN NATIVE INTELLIGENCE (NI) AND ARTIFICIAL INTELLIGENCE (AI): AN OVERVIEW by Bawallah Musa Adesola, Odole, Kehinde, Ilugbo, Stephen Olubusol, DareC, Ojo Omonijo, Owele Boye Agatha, Hadiza Mali Bukar

“…Western Ideologists tend to attribute Native Intelligence (NI) to Africans / third-word concepts, indistinctly fixed to value and belief that aided social cultural, religious, political economic, spiritual evolution, and ancient societies, However, the Emergence of Artificial intelligence (AI) being the capacity of a computer system or machine to mimic human cognitive such as learning and solving problems, through a computer system that uses mathematics and logic, to simulate the reasoning ability of man, through the process of learning from new information, and transforming such information into actions, by making decisions that has increasingly enhanced the quality of human life. …”
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BREEDING FOR ANTIOXIDANT CONTENT IN GRAIN AS A PROMISING TREND IN OBTAINING HEALTHY FOOD PRODUCTS by V. I. Polonskiy, I. G. Loskutov, A. V. Sumina

“…In recent years, research efforts have been started in a number of Western countries to analyze the content of antioxidants in the grain of various cereal crops. …”
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Effects of coenzyme q10 supplementation on metabolic and reproductive outcomes in obese rats by Gisela Belén Sarrible, María Victoria Bazzano, Caterina Koutsovitis, María Guillermina Bilbao, Rodrigo Hernán Da Cuña, Melanie Neira, Julián Alberto Bartolomé, Evelin Mariel Elia

“…The cafeteria diet (CAF) serves as an animal model mirroring Western diet habit. Coenzyme Q10 (CoQ10), known for enhancing reproductive outcomes in various pathologies, is not fully understood for its effects on obesity treatment. …”
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Influence of luteolin on the apoptosis of esophageal cancer Eca109 cells and its mechanism of action by Shaokang Wang, Lingmeng Fu, Yi Wu, Hongmei Xiao, Jing Wang, Guiju Sun

“…Expression of caspase9 and caspase3 mRNA and protein was analyzed by real-time PCR and Western blot respectively. The results showed that luteolin could inhibit the proliferation of Eca109 cells at all concentrations in a time-dependent manner and the relative inhibition rate showed an inverted U-shaped association with the concentration of luteolin. …”
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Carboxylesterase 4A Inhibits the Malignant Biological Behavior of Nasopharyngeal Carcinoma via the PI3K/AKT Pathway by Qiaoli Chen MS, Quanxiang Hao MBBS, Yanping Yang MBBS, Limei Li PhD, Danping Li MS, Ran Zhao MS, Wanqi Wei MBBS, Lixian Deng MBBS, Jiaming Su MBBS, Ziyuan Liang MBBS, Shiyue Tang MS, Yaomin Lu MBBS, Yushan Liang PhD, Zhe Zhang PhD, Xiaoying Zhou PhD, Xue Xiao PhD, Ping Li PhD, Yi Huang PhD, Weilin Zhao PhD

“…CCK-8, colony formation, wound healing and transwell assays were utilized to estimate cellular growth and metastasis, respectively. Western blot was conducted to evaluate the activity of PI3K/AKT signaling pathway. …”
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Oxymatrine inhibits the development of radioresistance in NSCLC cells by reversing EMT through the DcR3/AKT/GSK-3β pathway by Jianming Tang, Yu Cao, Hong Zhang, Rui Wang

“…Material and methods Western blotting was conducted to quantify the protein expression of epithelial-mesenchymal transition markers E-cadherin and vimentin in the A549 cell line. …”
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Lycium barbarum glycopeptide ameliorates aging phenotypes and enhances cardiac metabolism by activating the PINK1/Parkin-mediated mitophagy pathway in D-galactose-induced mice by Tianchan Peng, Jian Xiang, Yun Tian, Xiaogen Tang, Lina Wang, Lijuan Gao, Oscar Junhong Luo, Li’an Huang, Guobing Chen

“…Mitochondrial stress assays and JC-1 fluorescent probe were conducted to evaluate mitochondrial function. Flow cytometer and western blot were performed to assess mitophagy flux. …”
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