Systematic Administration of B Vitamins Alleviates Diabetic Pain and Inhibits Associated Expression of P2X3 and TRPV1 in Dorsal Root Ganglion Neurons and Proinflammatory Cytokines... by Duan-Duan He, Yu Gao, Shan Wang, Zhong Xie, Xue-Jun Song

“…DNP was produced in male, adult, Sprague Dawley rats by single i.p. streptozotocin (STZ). Western blot analysis and immunohistochemistry were used to analyze protein expressions in DRG and ELISA to measure the proinflammatory cytokines in the spinal cord. …”
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Glyphosate treatment mediates the accumulation of small discrete 5^′ and 3^′-terminal fragments of 18S rRNA in plant cells by A. V. Zhigailov, A. S. Nizkorodova, K. O. Sharipov, N. S. Polimbetova, B. K. Iskakov

“…In the present work, we studied the induction of 18S rRNA discrete fragmentation and phosphorylation of the α-subunit of eukaryotic initiation factor 2 (eIF2α) in germinated wheat embryos in the presence of glyphosate, which imitates the condition of amino acid starvation. Using northern and western blotting, we have shown that stress-induced 18S rRNA fragments started to accumulate in wheat embryos at glyphosate concentrations that did not evoke eIF2α phosphorylation. …”
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Clinical and Functional Characteristics of a Novel KLF11 Cys354Phe Variant Involved in Maturity-Onset Diabetes of the Young by Yujing Sun, Jingru Qu, Jing Wang, Ruxing Zhao, Chuan Wang, Li Chen, Xinguo Hou

“…Luciferase reporter assays were used to investigate whether the KLF11 variant binds to the insulin promoter. Real-time PCR, western blotting, and glucose-stimulated insulin secretion (GSIS) analysis were used to analyze the KLF11 variant that regulates insulin expression and insulin secretion activity in beta cell lines. …”
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MicroRNA-155 Participates in the Expression of LSD1 and Proinflammatory Cytokines in Rheumatoid Synovial Cells by Ziliang Yu, Hao Liu, Jianbo Fan, Feihu Chen, Wei Liu

“…Moreover, RT-qPCR was used to explore the relative LSD1 miRNA expression in FLS when stimulated by miRNA-155 molecules, and Western blot and immunofluorescence assay were applied to probe into the expression level of LSD1. …”
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KLF5 promotes esophageal squamous cell carcinoma radioresistance by targeting the Keap1-Nrf2 pathway by Yang Wang, Yue-ying Yang, Abulajiang Kamili, Dilimulati Aishanjiang, Yi Liu

“…The effects of overexpression or downregulation of KLF5 on anti-irradiated cells' proliferation, migration, invasion, and apoptotic activity were studied through colony formation assay, Transwell assay, and flow cytometry. Western blot can detect the activity of Nrf2 signaling pathway in cells and tissues. …”
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Ginsenoside Rg1 Regulates SIRT1 to Ameliorate Sepsis-Induced Lung Inflammation and Injury via Inhibiting Endoplasmic Reticulum Stress and Inflammation by Qian-Lu Wang, Lei Yang, Yue Peng, Min Gao, Ming-Shi Yang, Wei Xing, Xian-Zhong Xiao

“…For septic mice, lung tissue pathology was examined, plus ELISA assay for serum cytokines. Western blotting was used to examine the activation of inflammatory pathways and ER stress marker proteins in both cells and mouse lung tissues. …”
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In Vivo Tracking of Chemokine Receptor CXCR4-Engineered Mesenchymal Stem Cell Migration by Optical Molecular Imaging by Senthilkumar Kalimuthu, Ji Min Oh, Prakash Gangadaran, Liya Zhu, Ho Won Lee, Ramya Lakshmi Rajendran, Se hwan Baek, Yong Hyun Jeon, Shin Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol Ahn

“…CXCR4 mRNA and protein expression was confirmed by RT-PCR and Western blotting, respectively, and it was higher in MSC-CXCR4/Fluc2 than in naive MSCs. eGFP expression was confirmed by confocal microscopy. …”
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Macrophage P2X7 Receptor Function Is Reduced during Schistosomiasis: Putative Role of TGF-β1 by Suellen D’arc Santos Oliveira, Hayandra Ferreira Nanini, Luiz Eduardo Baggio Savio, Mariana Caldas Waghabi, Claudia Lucia Martins Silva, Robson Coutinho-Silva

“…TGF-β1 levels were increased in the peritoneal cavity of infected animals, and pretreatment of control macrophages with TGF-β1 reduced ATP-induced permeabilization, mimicking the effect of S. mansoni infection. Western blot and qRT-PCR data showed no difference in P2X7 protein and mRNA between uninfected, infected, and TGF-β1-treated groups. …”
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Inhibitory Effect of Flavonoid Extract of Lotus Leaf on Alcohol-Induced Gastric Injury by Antioxidant Capacity in Mice by Yi Chen, Qin Li, Zhiping Kuang, Xin Zhao, Ruokun Yi, Xiaowei He, Guangjing Feng

“…The quantitative polymerase chain reaction (qPCR) and Western blot results proved that FELL upregulated the mRNA and protein expressions of SOD1, SOD2, CAT, epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR), neuronal nitric oxide synthase (nNOS), and endothelial nitric oxide synthase (eNOS) and downregulated the expression of inducible nitric oxide synthase (iNOS) in the gastric tissue of mice with gastric injury. …”
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Effects of age and diet on triglyceride metabolism in mice by Kathryn M. Spitler, Shwetha K. Shetty, Brandon S.J. Davies

“…We fed male and female C57Bl/6 mice a low-fat diet, a western diet (WD), or a diet high in polyunsaturated and omega-3 fatty acids (n3D) for up to 2 years. …”
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Colonic Oxidative and Mitochondrial Function in Parkinson’s Disease and Idiopathic REM Sleep Behavior Disorder by C. Morén, Í. González-Casacuberta, J. Navarro-Otano, D. Juárez-Flores, D. Vilas, G. Garrabou, J. C. Milisenda, C. Pont-Sunyer, M. Catalán-García, M. Guitart-Mampel, E. Tobías, F. Cardellach, F. Valldeoriola, A. Iranzo, E. Tolosa

“…Oxidative damage was assessed either by lipid peroxidation, through malondialdehyde and hydroxyalkenal content by spectrophotometry, or through antioxidant enzyme levels of superoxide dismutase-2 (SOD2), glutathione peroxidase-1 (Gpx1), and catalase (CAT) by western blot. The presence of mitochondrial DNA (mtDNA) deletions was assessed by long PCR and electrophoresis. …”
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Impact of Notch3 Activation on Aortic Aneurysm Development in Marfan Syndrome by Kathryn Jespersen, Chenxin Li, Rishi Batra, Christopher A. Stephenson, Paul Harding, Kylie Sestak, Ryan T. Foley, Harrison Greene, Trevor Meisinger, Jason R. Cook, B. Timothy Baxter, Wanfen Xiong

“…Notch 3 expression and activation in aortic tissue were confirmed with real-time RT-PCR, immunohistochemistry, and Western blot. Fbn1mgR/mgR and wild-type mice were treated with a γ-secretase inhibitor, DAPT, to block Notch activation. …”
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Electroacupuncture combined with trigonelline inhibits pyroptosis in cerebral ischemia-reperfusion by suppressing autophagy via the PI3K/AKT/mTOR signaling pathway by Zhengguo Qiu, Jianbing Ma, Xiaqing Zhang, Mingna Jiao, Liqiang Zhi

“…The expression of various proteins was measured using IF assay and western blot. Results: EA or TG treatment could effectively ameliorate neurological disorders, attenuate cerebral infarction and reduce neuronal damage in brain tissue in CIRI rats. …”
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6-Gingerol Normalizes the Expression of Biomarkers Related to Hypertension via PPARδ in HUVECs, HEK293, and Differentiated 3T3-L1 Cells by Yong-Jik Lee, Yoo-Na Jang, Yoon-Mi Han, Hyun-Min Kim, Hong Seog Seo

“…The antihypertensive effects and mechanism of 6-gingerol were identified through reverse transcription polymerase chain reaction (RT-PCR), western blotting, and immunocytochemical staining for biomarkers involved in hypertension in human umbilical vein endothelial cells (HUVECs), human embryonal kidney cells (HEK293 cells), and mouse preadipocytes (3T3-L1 cells). …”
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Drought resistance of introgressive spring common wheat lines with genetic material of tall wheatgrass by L. Ya. Plotnikova, A. T. Sagendykova, S. P. Kuzmina

“…The introgressive lines of spring common wheat with T. ponticum genetic material and standard cultivars were studied in the field in the southern forest-steppe of Western Siberia using generally recognized methods. …”
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Epidermal stem cell derived exosomes-induced dedifferentiation of myofibroblasts inhibits scarring via the miR-203a-3p/PIK3CA axis by Shixin Zhao, Haoran Kong, Dahu Qi, Yushuang Qiao, Yu Li, Zhiming Cao, Hanwen Wang, Xuefeng He, Hengdeng Liu, Hao Yang, Suyue Gao, Tao Liu, Julin Xie

“…After in vitro and in vivo treatment with EpiSC-EVs, we assessed antifibrotic effects through scratch assays, collagen contraction assays, Western blotting, and immunofluorescence. Transcriptomic sequencing and rescue experiments were used to investigate the molecular mechanism by which miR-203a-3p in EpiSC-EVs induces myofibroblast dedifferentiation. …”
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SOX7 is involved in polyphyllin D-induced G0/G1 cell cycle arrest through down-regulation of cyclin D1 by Zheng Bin, Wang Gang, Gao Wenbo, Wu Qiquan, Zhu Weizhi, Weng Guobin

“…Cell cycle progression was analyzed using propidium iodide (PI) staining, and protein levels were assayed by Western blot analysis. Our results showed low expression of SOX7 in PCa tissues/cells compared to their non-tumorous counterparts/RWPE-1 cells. …”
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M6a demethylase FTO regulates the oxidative stress, mitochondrial biogenesis of cardiomyocytes and PGC-1a stability in myocardial ischemia-reperfusion injury by Qiong Jiang, Xuehai Chen, Kezeng Gong, Zhe Xu, Lianglong Chen, Feilong Zhang

“…Studying the molecular mechanism of MIRI and discovering new targets are crucial for the future treatment of MIRI.Methods We constructed the MIRI rat model and hypoxia/reoxygenation (H/R) injury cardiomyocytes model. RT–PCR and Western blot were used to investigate the expression of the fat mass and obesity-associated (FTO) gene. …”
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Expression and Significances of Contactin-1 in Human Gastric Cancer by Ji-Wei Yu, Sheng-Hua Wu, Rui-qi Lu, Ju-gang Wu, Xiao-Chun Ni, Gou-cai Zhou, Hai-guang Jiang, Lin-Hai Zheng, Xiao-Qiang Li, Guang-Ye Du, Bo-Jian Jiang

“…The expression level of CNTN-1 mRNA and CNTN-1 protein of 33 cases was determined using RT-PCR and Western Blot. And 105 cases were immunohistochemically examined for VEGF-C, VEGFR-3, and CNTN-1 expressions. …”
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Autophagy inhibitor 3-methyladenine mitigates the severity of colitis in aged mice by inhibiting autophagy by Ailing Liu, Hongying Wang, Hong Lv, Hong Yang, Yue Li, Jiaming Qian

“…The autophagy activity was detected by the protein expressions of LC3B-II, p62 and ATG5 by western blot and immunohistochemistry. The levels of TNF -α, IL-6, CCL4, CXCL12 and CD86 were measured by qRT-PCR. …”
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