Mechanism of action of Xipayimaizibizi oral liquid on outlet obstruction-induced overactive bladder: An integrated study by Menglu Wang, Yang Yang, Yuhang Du, Jiamei Xie, Yige Zhao, Yongcheng An, Ziyi Shan, Shenyujun Wang, Meng Hao, Baosheng Zhao

“…The key targets involved in these mechanisms were validated using reverse transcription-quantitative polymerase chain reaction, immunohistochemistry, and western blot in vivo/vitro experiments. Result: Network pharmacological analysis predicted that XP may alleviate OAB by affecting the cholinergic synapse and calcium signaling pathways. …”
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Transformer-based travel time estimation method for plateau and mountainous environments by Guangjun Qu, Kefa Zhou, Rui Wang, Dong Li, Yingpeng Lu, Zhihong Lv, Dequan Zhao, Aijun Zhang, Qing Zhang

“…Two datasets were constructed based on measurements from two selected areas in eligible plateau and mountainous regions of western China. For each dataset, two categories of features were defined: terrain-weather features and spatio-temporal features. …”
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ASTEC–MAAP Comparison of a 2 Inch Cold Leg LOCA until RPV Failure by J. C. de la Rosa Blul, S. Brumm, F. Mascari, S. J. Lee, L. Carenini

“…A 2 inch, cold-leg loss-of-coolant accident (LOCA) in a 900 MWe generic Western PWR was simulated using ASTEC 2.1.1 and MAAP 5.02. …”
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Tanshinone IIA Sodium Sulfonate Attenuates LPS-Induced Intestinal Injury in Mice by Xin-Jing Yang, Jin-Xian Qian, Yao Wei, Qiang Guo, Jun Jin, Xue Sun, Sheng-Lan Liu, Chun-Fang Xu, Guo-Xing Zhang

“…In addition, expressions of autophagy markers (microtubule-associated light chain 3 (LC3) and Beclin-1) were detected by western blot and RT-PCR. A number of autophagosomes were also observed under electron microscopy. …”
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Prognostic Value of PSMB5 and Correlations with LC3II and Reactive Oxygen Species Levels in the Bone Marrow Mononuclear Cells of Bortezomib-Resistant Multiple Myeloma Patients by Eva Plakoula, Georgios Kalampounias, Spyridon Alexis, Evgenia Verigou, Alexandra Kourakli, Kalliopi Zafeiropoulou, Argiris Symeonidis

“…PSMB5 and LC3I/II protein levels were determined using Western blot, proteasome proteolytic activity (PPA) with spectrofluorometry, and ROS with flow cytometry. …”
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ATP Induces Disruption of Tight Junction Proteins via IL-1 Beta-Dependent MMP-9 Activation of Human Blood-Brain Barrier In Vitro by Fuxing Yang, Kai Zhao, Xiufeng Zhang, Jun Zhang, Bainan Xu

“…Permeability was used to evaluate changes in the integrity of TJPs. ELISA, Western blot, and immunofluorescent staining procedures were used. …”
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The critical role of MLKL in hemorrhagic stroke and the therapeutic potential of its associated protein network by Yi Wang, Yi Wang, Moran Xu, Xiaoli Zuo, Sheng Wang, Yong Yu, Zhaobing Gao, Zhaobing Gao, Jingbo Qie, Ye Jiang, Fang Huang, Bingqing Xia, Bingqing Xia

“…Subsequently, we investigated the impact of MLKL knockout on ICH pathological damage through behavioral tests, Western blotting, and RT-qPCR. Finally, we performed a proteomic analysis via LC-MS/MS to explore the potential interacting proteins of MLKL in the progression of ICH.ResultsWe found that MLKL is highly expressed in the brain tissue of ICH patients and is positively correlated with the extent of injury. …”
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Effects of Ghrelin on Triglyceride Accumulation and Glucose Uptake in Primary Cultured Rat Myoblasts under Palmitic Acid-Induced High Fat Conditions by Lingling Han, Jia Li, Ying Chen, Wei Wang, Dan Zhang, Guoliang Liu

“…The glucose transporter 4 (GLUT4), AMP-activated protein kinase (AMPK), acetyl-CoA carboxylase (ACC), and uncoupling protein 3 (UCP3) were assessed by RT-PCR and western blot. Compared to 0.3 mM PA, ghrelin at 10−9 and 10−7 M reduced triglyceride content (5.855 ± 0.352 versus 5.030 ± 0.129 and 4.158 ± 0.254 mM, P<0.05) and prevented PA-induced reduction of glucose uptake (1.717 ± 0.264 versus 2.233 ± 0.333 and 2.333 ± 0.273 10−2 pmol/g/min, P<0.05). …”
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Public Sector Reform from the Post-New Public Management Perspective: Review and Bibliometric Analysis by Marko Ropret, Aleksander Aristovnik

“…Furthermore, we have shown that such evaluation of governance and related doctrines may be biased in favour of subjective, pluralistic Western ideas about governance, presumably limiting their impact within the CEE and several other regions. …”
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Clinical application of oral Chinese Patent Medicines in ophthalmology: a scoping review protocol by Wei Zhou, Kai Xu, Ziyang Huang, Xiaoyu Li, Xing Liao, Lina Liang, Yamin Li, Qianqian Zuo

“…In recent years, CPMs have been increasingly used in ophthalmology clinics by TCM practitioners and by Western doctors in general hospitals. However, comprehensive evidence for using CPMs in ophthalmology is lacking.Aim We will apply the methodology of scoping review to systematically search and sort out the available evidence on oral CPMs for the treatment of eye diseases, identify the distribution of evidence in this field and provide a basis for clinical practice and medical decisions.Methods The scoping review will be implemented in the following seven steps: (1) defining the research question; (2) searching National Essential Medicines List (2018 edition), National Basic Medical Insurance, Work Injury Insurance and Maternity Insurance Drug Catalog (2020 edition) and Chinese Pharmacopoeia (2020 edition) for oral CPMs for the treatment of eye diseases; (3) searching Embase, Web of Science, PubMed, Cochrane Library, Chongqing VIP Chinese Scientific Journals Database, Chinese National Knowledge Infrastructure, Chinese Biomedical Literature Database and Wanfang Database for relevant literature published from inception to December 2021; (4) developing eligibility criteria; (5) screening the studies based on inclusion criteria; (6) extracting relevant data and lastly, (7) collating, summarising and reporting the results.Ethics and dissemination Since the scoping review aims at collecting data from publicly available publications, this study does not require ethical approval. …”
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Spatiotemporal changes of ecosystem health and the impact of its driving factors on the Loess Plateau in China by Xuepeng Zhang, Taixia Wu, Qiqi Du, Ninglei Ouyang, Wei Nie, Yang Liu, Peng Gou, Guangchao Li

“…Counties with higher response to TEM (>0.26) are primarily in the southern and western regions. The results of this paper provide new insights for EH research in areas with fragile ecosystems and are of significant importance for the ecological civilization construction of the Loess Plateau.…”
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Antimicrobial Usage in Smallholder Poultry Production in Nigeria by Oladeji Bamidele, Tunde A. Amole, Oluwafikayo A. Oyewale, Olayinka O. Bamidele, Abdulmojeed Yakubu, Uduak E. Ogundu, Folasade O. Ajayi, Waheed A. Hassan

“…The practice of EVM was prevalent among most of the farmers (39%). The western method (pharmaceuticals) was practiced by a large proportion of farmers (60%), either solely (25%) or in combination with EVM (35%). …”
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A novel tissue culture method to produce professional antigen-presenting cells from rainbow trout in vitro by Nguyen T. K. Vo, PhD, Lital Sever, PhD, Brian Dixon, PhD, Niels C. Bols, PhD

“…Using affinity-purified anti-trout MH IIα and MH IIβ primary antibodies that were generated in house, abundant levels of MH IIα and MH IIβ polypeptides were detected in fin-LLCs by Western blotting. Immunocytochemistry in combination with confocal microscopy showed that DC-like cells had more cell-surface MH II proteins than macrophage-like cells. …”
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Tensile force promotes osteogenic differentiation via ephrinB2-EphB4 signaling pathway in orthodontic tooth movement by Hang Yu, Xiaoxi Wei, Huan Jiang, Huichuan Qi, Yi Zhang, Min Hu

“…Levels of osteogenic differentiation-related factors were detected by ALP staining, ARS staining, qPCR and western blot. NVP-BHG712 was used to block EphB4 receptor. …”
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Lnc-EST885 promotes hepatocellular carcinoma metastasis through PI3K / AKT pathway by interaction with TRAF4 by Shaoliang Zhu, Gang Wang, Yuxuan Zhang, Mengjie Zou, Zhi Li, Shenhong Qu, Xiaosu Zou, Wenqian Nong, Weiwei Miao, Qicong Chen, Juanmei Mo, Huibing Chen, Lequn Li, Xiaofeng Dong, Honglin Luo

“…Methods: Cell experiments including FISH, western blot, flow cytometry and functional analysis were used to elucidate the effects of lnc-EST885 on cell proliferation, apoptosis, migration and EMT processes. …”
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Study of subterranean biodiversity of the upper Neretva River catchment in Bosnia and Herzegovina by Maja Zagmajster, Ester Premate, Špela Borko, Behare Rexhepi, Vojo Milanović, Anton Brancelj, Michaela Brojer, Christophe Douady, Josef Grego, Milica Jovanović, Florian Malard, Nataša Mori, Vladimir Pešić

“… The Dinarides in the Western Balkans host a globally exceptional subterranean biodiversity, but still areas remain with little or no data on subterranean species. …”
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Cyanin Chloride Inhibits Hyperbaric Pressure-Induced Decrease of Intracellular Glutamate-Aspartate Transporter in Rat Retinal Müller Cells by Xiaomin Chen, Yue Wang, Fangfang Han, Min Ke

“…GLAST mRNA and protein expression were determined by western blot and RT-PCR analyses, respectively. Results. …”
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A unique mixture of monoterpenes and volatile phenols characterises Zelen wine’s aromatic profile by Mitja Martelanc, Davide Slaghenaufi, Tatjana Radovanović Vukajlović, Diana Alexandra Martin Rojas, Katja Suklje, Melita Sternad Lemut, Branka Mozetič Vodopivec, Lorena Butinar, Guillaume Antalick

“… This study aimed to characterize the aromatic uniqueness of Zelen (Vitis vinifera L.) wines, an authoctonous variety from Vipava Valley located in Western Slovenia, through chemical and sensory assessment. …”
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HDAC Inhibition Modulates Cardiac PPARs and Fatty Acid Metabolism in Diabetic Cardiomyopathy by Ting-I Lee, Yu-Hsun Kao, Wen-Chin Tsai, Cheng-Chih Chung, Yao-Chang Chen, Yi-Jen Chen

“…Electrocardiography, echocardiography, and western blotting were used to evaluate the electrophysiological activity, cardiac structure, fatty acid metabolism, inflammation, and PPAR isoform expressions in the control and streptozotocin-nicotinamide-induced DM rats with or without MPT0E014. …”
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Electroacupuncture Alleviates Neuropathic Pain through Regulating miR-206-3p Targeting BDNF after CCI by Wenzhan Tu, Jingjing Yue, Xuqing Li, Qiaoyun Wu, Guanhu Yang, Shengcun Li, Qiangsan Sun, Songhe Jiang

“…Mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) tests were used to determine differences in neurobehavioral manifestations. qPCR, western blotting, and immunofluorescence (IF) were carried out to detect the expression levels of miR-206-3p, BDNF, BAX/Bcl-2, TNF-α, and IL-6. …”
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