METHOD FOR EFFECTIVE SEPARATION OF MICROPLASTIC PARTICLES FROM BOTTOM SEDIMENTS by V.O. Iemelianov, Ye.І. Nasiedkіn, T.S. Kukovska, S.M. Dovbysh

“…The SSI "MorGeoEcoCenter of the National Academy of Sciences of Ukraine" within the framework of the international project "Developing Optimal and Open Research Support" for the Black Sea (DOORS) (https://www.doorsblacksea.eu/) conducts research on the distribution of microplastics in the bottom sediments of the shelf areas of the western parts, as well as coastal deposits of the northwestern part of the Black Sea. …”
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Mine Geological Environment Monitoring and Risk Assessment in Arid and Semiarid Areas by Jiaqi Jin, Chicheng Yan, Yixuan Tang, Yilong Yin

“…Along with the accelerated shift of coal mining to the ecologically fragile west, the contradiction between coal resource development and ecological protection in the western arid and semiarid coal mining areas is rapidly intensifying. …”
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Using Network Pharmacology and Animal Experiment to Investigate the Therapeutic Mechanisms of Polydatin against Vincristine-Induced Neuropathic Pain by Peng Xi, Rui Mao, Shiyan Wu, Lei Liu, Ceng Cai, Lei Lu, Cailin Zhang, Yimei Li

“…The results of qRT-PCR, western blot, and immunohisochemistry indicated that PD inhibited the IL-6, TP53, and MAPK1 levels in VIN-induced neuropathic pain rats. …”
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A Comparison of Machine Learning-Based Approaches in Estimating Surface PM_2.5 Concentrations Focusing on Artificial Neural Networks and High Pollution Events by Shijin Wei, Kyle Shores, Yangyang Xu

“…The sensitivity analysis of features that interpret the models suggests that the total extinction AOD at 550 nm and surface CO concentrations are the most important features in the Western and Eastern U.S., respectively. The findings suggest that even the simplest NNs provide better air quality estimates, especially during high pollution events, which is beneficial for long-term exposure analysis. …”
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The Body as a Vessel for Trauma: The Clinical Case Study of Aisha by Federica Visco-Comandini, Alberto Barbieri, Francesco Mancini, Alessandra Ciolfi

“…The treatment was provided in a Western country. The client’s improvement was assessed by using self-report assessments of post-traumatic, dissociative, and depressive symptoms. …”
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Mediterranean refugee traffic: main routes and problems of control by Vasily D. Ostanin-Golovnya, Anna A. Pavliukova

“…The most popular of them is the Central Mediterranean route, which runs from Tunisia and Libya, then the Western Mediterranean route – from Morocco and Algeria. …”
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Hypoxia Promotes Glioma Stem Cell Proliferation by Enhancing the 14-3-3β Expression via the PI3K Pathway by Weidong Cao, Qiang Zhou, Hongwei Wang, Wei Rao, Gang Cheng, Peng Wang, Shengli Guo, Bin Ren, Jianning Zhang

“…Hypoxia induced by using CoCl2 application and 14-3-3β protein knockdown by specific small interfering RNA transfection were performed in GSCs both in vitro and in vivo to observe their role in glioma progression and metastasis occurrence by using western blot analysis and MTT assay. The results demonstrated that CoCl2 application enhanced the 14-3-3β protein expression and mRNA levels via the PI3K pathway in GSCs. …”
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The Histone Demethylase KDM3B Promotes Osteo-/Odontogenic Differentiation, Cell Proliferation, and Migration Potential of Stem Cells from the Apical Papilla by Chen Zhang, Xiao Han, Yuncun Liang, Huina Liu, Zhipeng Fan, Jianpeng Zhang

“…By an alkaline phosphatase (ALP) activity assay, Alizarin red staining, real-time RT-PCR, and western blot analysis, we found that KDM3B enhanced the ALP activity and mineralization of SCAPs and promoted the expression of runt-related transcription factor 2 (RUNX2), osterix (OSX), dentin sialophosphoprotein (DSPP), and osteocalcin (OCN). …”
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The Active Compound Thymoquinone Alters Chondrogenic Differentiation of Human Mesenchymal Stem Cells via Modulation of Intracellular Signaling by Banu ISKENDER

“…The effects of thymoquinone on hMSC differentiation and the relevant intracellular signaling pathways were determined using immunocytochemistry and western blotting for the first time. Changes in the phosphorylation status of some signaling components involved in NF-κB and mTOR signaling were also evaluated. …”
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Anti-Inflammatory Effect of Columbianetin on Lipopolysaccharide-Stimulated Human Peripheral Blood Mononuclear Cells by Junying Lu, Keyong Fang, Shiji Wang, Lingxin Xiong, Chao Zhang, Zhongmin Liu, Xuewa Guan, Ruipeng Zheng, Guoqiang Wang, Jingtong Zheng, Fang Wang

“…To further confirm the targeted pathway of CBT, we pretreated PBMC with the selective NOD1 inhibitor ML130 and then measured the protein levels of the pathway by Western blotting. The result showed that CBT effectively suppressed the expressions of TNF-α, IL-6, MCP-1, and IL-1β in a dose-dependent manner and significantly downregulated 19 out of 32 differentially expressed genes, most of which were involved in the NOD1/NF-κB pathway, and also showed that CBT remarkably inhibited LPS-induced NOD1, RIP2, and NF-κB activation. …”
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Patchoulene Epoxide Isolated from Patchouli Oil Suppresses Acute Inflammation through Inhibition of NF-κB and Downregulation of COX-2/iNOS by Jia-Li Liang, Jia-Zhen Wu, Yu-Hong Liu, Zhen-Biao Zhang, Qi-Duan Wu, Han-Bin Chen, Yan-Feng Huang, Yao-Xing Dou, Jiang-Tao Zhou, Zi-Ren Su, Janis Ya-Xian Zhan

“…PAO was also shown to significantly downregulate the protein and mRNA expressions of cyclooxygenase-2 (COX-2) and inducible nitric-oxide synthase (iNOS). Western blot analysis revealed that PAO remarkably inhibited p50 and p65 translocation from the cytosol to the nucleus by suppressing IKKβ and IκBα phosphorylation. …”
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METTL3-Mediated m6A Modification Regulates the Osteogenic Differentiation through LncRNA CUTALP in Periodontal Mesenchymal Stem Cells of Periodontitis Patients by Xin Chen, Yuan Qin, Xian Wang, Hao Lei, Xiaochen Zhang, Houzhuo Luo, Changgang Guo, Weifu Sun, Shishu Fang, Wen Qin, Zuolin Jin

“…We carried out a series of experiments, including methylated RNA immunoprecipitation-PCR, quantitative real-time polymerase chain reaction, and western blotting. The expressions of alkaline phosphatase (ALP), Runx2, Col1, Runx2 protein level, ALP staining, and Alizarin red staining were used to demonstrate the degree of osteogenic differentiation. …”
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Preparation, characterisation, and in vitro cancer‐suppression function of RNA nanoparticles carrying miR‐301b‐3p Inhibitor by Junjie Zhao, Niu Niu, Fan Yang, Haitao Liu, Weibo Qi

“…Cell internalisation, toxicity, proliferation, migration, invasion, and apoptosis were assayed by confocal laser scanning microscope, CCK8, colony formation assay, Transwell, western blot, and flow cytometry. Results 3WJ‐apt‐miR was evenly distributed, with diameter of 19.61 ± 0.49 nm and triangular branching structures. …”
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Bradykinin Type-2 Receptor Expression Correlates with Age and Is Subjected to Transcriptional Regulation by Inka Liesmaa, Naotaka Shiota, Jorma O. Kokkonen, Petri T. Kovanen, Ken A. Lindstedt

“…Heart tissues from idiopathic dilated cardiomyopathy (IDC; 𝑛=7), coronary heart disease (CHD; 𝑛=6), and normal patients (𝑛=6) were analyzed by RT-PCR, SSCP, and Western blotting. In normal and IDC hearts, BK-2R expression increased with age, with a lower relative increase in IDC hearts. …”
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Update on the Treatment of Diabetic Retinopathy by Jennifer L. Wilkinson-Berka, Antonia G. Miller

“…This situation is highlighted by the frightening statistic that diabetic retinopathy (DR) remains the most common cause of vision impairment in people of working age in Western society. With the global epidemic of type 2 diabetes, this predicament is set to worsen as over 360 million people are projected to suffer from diabetes and its complications by 2030. …”
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Determination of Drought and Wet Periods of the Black Sea Region Using SPI Method by Yasemin Deniz Öztürk, Ramazan Ünlü

“…According to the Mann-Kendall trend analysis results, it was not found that there was a decreasing trend in the coastal areas of the Western Black Sea Region. However, there was no significance in the decrease. …”
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Acanthopanax versus 3-Methyladenine Ameliorates Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting the Autophagic Pathway in Rats by Xiaohong Wang, Guoxiong Zhou, Chun Liu, Ronglong Wei, Shunxing Zhu, Yuefen Xu, Mengjie Wu, Qing Miao

“…Serum amylase levels were determined by ELISA; protein and mRNA expression levels of nucleus nuclear factor kappa B (NF-κB) p65, light chain 3II (LC3-II), and Beclin-1 and mRNA expression levels of Class III phosphatidylinositol 3-kinase (PI3K-III) in pancreas tissue were detected by Western blot and quantitative real-time PCR, respectively; mortality and pathological change of the pancreas were observed at 3, 12, and 24 h after operation. …”
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STAT3-Inducible Mouse ESCs: A Model to Study the Role of STAT3 in ESC Maintenance and Lineage Differentiation by Yu Qian Wong, Hongyan Xu, Qiang Wu, Xinyu Liu, Chengchen Lufei, Xiu Qin Xu, Xin-Yuan Fu

“…Through genotyping, deletion of STAT3 alleles was detected in InSTAT3 KO ESCs following 24 hours Dox stimulation. Western blot also showed that pSTAT3 and STAT3 protein levels were significantly reduced in InSTAT3 KO ESCs while dominantly elevated in InSTAT3 CA ECSs upon Dox stimulation. …”
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SNP rs9364554 Modulates Androgen Receptor Binding and Drug Response in Prostate Cancer by Yuqian Yan, Lei Shi, Tao Ma, Liguo Wang, Haojie Huang

“…We verified onco-mining findings in prostate cancer cell lines using quantitative PCR and Western blots. Additionally, we employed electrophoretic mobility shift assay (EMSA) to detect the binding affinity of transcription factors to this SNP. …”
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24-Hour Measurement of Gastric pH in Rural South Africa by Alastair M. Sammon, Eugene J. Ndebia, Ekambaram Umapathy, Jehu E. Iputo

“…Previous studies have established norms of 24-hour gastric pH profiles for western countries. This study was designed to establish the pattern for a rural African population with a high incidence of oesophageal cancer. …”
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