Natural population cohort study on long-lived adults: West China longevity and ageing procedure (WCLAP) by Li Wang, Yan Zhang, Qiukui Hao, Xiaoyan Chen, Birong Dong, Yan He, Meijuan Chen, Jirong Yue, Xiaochu Wu, Tianyao Zhang, Yan She, Yanling Gao, Yiping Deng

“…Purpose The West China longevity and ageing procedure (WCLAP) cohort study aims to provide guidance for older adults in western China with the aim of improving quality of life, reducing the burden of family care, summarising the characteristics of longevity lifestyles, building a Chinese-longevity-population biobank and exploring the mechanisms underlying population ageing.Participants Since the establishment of the WCLAP research baseline in 2018, a population of 1537 adults aged 80 years and above, living in the community, have been enrolled in the programme as research participants. …”
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GENI as an AMPK Activator Binds α and γ Subunits and Improves the Memory Dysfunction of Alzheimer’s Disease Mouse Models via Autophagy and Neuroprotection by Ying Wang, Lanjie Li, Danni Chen, Jiaheng Shan, Meijuan Yi, Hiroyuki Osada, Minoru Yoshida, Lan Xiang, Jianhua Qi

“…In this study, to indicate whether this compound has anti-Alzheimer’s disease (AD) effect, the galactose-induced AD mice and naturally aging mice with AD were used to do drug efficacy evaluation. Furthermore, the Western blot, small interfering RNA (siRNA), drug affinity responsive target stability (DARTS), cellular thermal shift assay (CESTA), liquid chromatography-tandem mass spectrometry (LC/MS-MS), adenosine 5′-monophosphate-activated protein kinase (AMPK) mutants and surface plasmon resonance (SPR) analysis were utilized to clarify the mechanism of action and identify target protein of this molecule. …”
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Climate sensitivity and attribution analysis of water resources in China by Youzhu Zhao, Qiuxiang Jiang, Zilong Wang

“…Results indicate that TWS in eastern China exhibits high sensitivity to precipitation, while TWS in the central areas and parts of the western border areas is more sensitive to temperature changes. …”
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Bisphenol S Induces Lipid Metabolism Disorders in HepG2 and SK-Hep-1 Cells via Oxidative Stress by Kai-Xing Lin, Zi-Yao Wu, Mei-Lin Qin, Huai-Cai Zeng

“…Alanine aminotransferase (ALT), T-CHO, and low-density lipoprotein cholesterol (LDL-C) levels also increased significantly after 24 or 48 h BPS treatments (<i>p</i> < 0.05). RT-PCR and Western blot analyses detected mRNA or protein expression levels of peroxisome proliferator-activated receptor α (PPARα) and sterol regulatory element-binding protein 1c (SREBP1C). …”
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Crop water productivity assessment and planting structure optimization in typical arid irrigation district using dynamic Bayesian network by Yantao Ma, Jie Xue, Xinlong Feng, Jianping Zhao, Junhu Tang, Huaiwei Sun, Jingjing Chang, Longke Yan

“…Based on the spatial distribution of crop suitability analysis, wheat and corn are most suitable for cultivation in the central and eastern parts of Qira oasis while cotton is unsuitable for planting in the western region. The walnuts and Chinese dates are mainly unsuitable in the southeastern part of the oasis. …”
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A Case Study on Strategic Multi-Objective Optimization of Influenza Vaccine Allocation: Age-Specific Approaches in Saudi Arabia by Asma K. Alkhamis, Manar I. Hosny

“…The research divides the population into five age categories&#x2014;infants and children, adolescents, young adults, middle-aged adults, and seniors&#x2014;across five regions: Eastern, Central, Northern, Western, and Southern Saudi Arabia. The study compares three vaccination strategies: no vaccination, national vaccination, and a two-phased multi-objective optimization approach under various pandemic scenarios. …”
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Dual inhibition of BET and EP300 has antitumor activity in undifferentiated pleomorphic sarcomas and synergizes with ferroptosis induction by Stéphanie Verbeke, Aurélien Bourdon, Mathilde Lafon, Vanessa Chaire, Bertolo Frederic, Amina Naït Eldjoudi, Marie-Alix Derieppe, Francis Giles, Antoine Italiano

“…The most potent inhibitor was evaluated in vivo in two animal models and the mechanisms of action were investigated by RNA sequencing, Western blotting and immunofluorescence staining. …”
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Human parietal epithelial cells as Trojan horses in albumin overload by Giovanna Priante, Monica Ceol, Lisa Gianesello, Claudia Maria Radu, Rachele Mantese, Lucia Federica Stefanelli, Martina Cacciapuoti, Francesca K. Martino, Lorenzo Arcangelo Calò, Franca Anglani, Federico Nalesso, Dorella Del Prete

“…Protein expression was assessed using In-Cell Western and immunofluorescence. Cell morphology was analyzed by phase-contrast microscopy and F-actin staining. …”
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Nicorandil Ameliorates Depression‐Like Behaviors After Traumatic Brain Injury by Suppressing Ferroptosis Through the SLC7A11/GPX4 Axis in the Hippocampus by Yao‐Ran Tu, Ming Tan, Yao Li, De‐Quan Hong, Fan Niu

“…The SLC7A11/GPX4 axis was analyzed using quantitative real‐time PCR (qRT‐PCR) and Western blot analysis. Results Nicorandil administration significantly ameliorated depression‐like behaviors in rats with TBI. …”
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Effects of Simvastatin on Glucose Metabolism in Mouse MIN6 Cells by Jieqiong Zhou, Weihua Li, Qiang Xie, Yuxi Hou, Shaopeng Zhan, Xi Yang, Xiaofeng Xu, Jun Cai, Zhengrong Huang

“…Luciferase method was used to examine the content of ATP in MIN6 cells. Real-time PCR and western blotting were performed to measure the mRNA and protein levels of inward rectifier potassium channel 6.2 (Kir6.2), voltage-dependent calcium channel 1.2 (Cav1.2), and glucose transporter-2 (GLUT2), respectively. …”
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Tolerance of high-yielding corn hybrids to drought stress during the early growth stage by Jiovana Kamila Vilas-Boas, Fábio Steiner, Alan Mario Zuffo, Jorge González Aguilera, Charline Zaratin Alves

“…Fourteen corn hybrids commonly grown in the central-western region of Brazil, in Cassilândia, Mato Grosso do Sul, were assessed for drought tolerance during the early plant growth stage. …”
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Exploring the Anticancer Potential of MonoHER (7-Mono-O-(β-Hydroxyethyl)-Rutoside): Mitochondrial-Dependent Apoptosis in HepG2 Cells by Chujie Li, Yue Wang, Jian Liang, Guido R. M. M. Haenen, Yonger Chen, Zhengwen Li, Ming Zhang, Ludwig J. Dubois

“…The effects of monoHER on apoptosis proteins were detected by confocal microscopy analysis and Western blot. Results: It was found that MonoHER can reduce HepG2 cells’ and MCF7 cells’ viability, but not H1299 cells’, and induced apoptosis only in HepG2 cells. …”
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Effect of baseline values on inpatient rehabilitation outcomes after total knee arthroplasty: a retrospective observational study by Martin Missmann, Michael J. Fischer

“…Methods: Patients participated in 3-week inpatient rehabilitation following TKA and were assessed for patient-reported outcome measures (PROMs), which included the Numeric Pain Rating Scale (NPRS), the Health Assessment Questionnaire (HAQ), the European Quality of Life 5 Dimensions 5 Level Version (EQ-5D-5L), and the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC). …”
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Dihydromyricetin inhibits injury caused by ischemic stroke through the lncRNA SNHG17/miR-452-3p/CXCR4 axis by Jiacheng Xie, Qiuyue Yang, Xueliang Zeng, Qi Zeng, Hai Xiao

“…The mechanism of action of DHM was explored by RT-qPCR, ELISA, luciferase reporter gene assay and western blotting. DHM dose-dependently enhanced BV2 cell viability post-OGD/R and attenuated inflammation and oxidative stress. …”
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Low seismic shaking induced densification of large spoil heap composed with low-density particle material by Jian Lv, Tao Jiang, Shuangcheng Wang, Wei Huang, Shenghua Cui, Lihu Duan, Zhenfu Huang

“…To investigate the vibration-induced densification characteristics and deformation mechanisms of a low-density spoil heap under small seismic effects, a spoil heap located in western Sichuan Province was selected as the research subject, and a large-scale shaking table test was performed.MethodsThe settlement and acceleration response behaviors of the slope model during the vibration process were analyzed using three-dimensional point cloud data and acceleration time-history data, with horizontal and horizontal-vertical bi-directional seismic waves applied at progressively increasing amplitudes. …”
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Sparse seagrass meadows are critical dugong habitat: A novel rapid assessment of habitat-wildlife associations using paired drone and in-water surveys by Nicole E. Said, Christophe Cleguer, Paul Lavery, Amanda J. Hodgson, Connor Gorham, Julian A. Tyne, Ankje Frouws, Simone Strydom, Johnny Lo, Holly C. Raudino, Kelly Waples, Kathryn McMahon

“…Here we integrate the use of small drones for dugong surveys with underwater benthic habitat assessment techniques at the local spatial scale (∼30 km2), to determine the drivers of dugong (Dugong dugon) distribution across three locations in the Pilbara, Western Australia. Paired assessment data was collected three times over two years. …”
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Comparing High-Intensity Interval Training (HIIT) and Continuous Training on Apelin, APJ, NO, and Cardiotrophin-1 in Cardiac Tissue of Diabetic Rats by Mostafa Sabouri, Javad Norouzi, Yashar Zarei, Mojtaba Hassani Sangani, Babak Hooshmand Moghadam

“…Apelin, APJ receptor, NO, and cardiotrophin-1 protein expressions were measured using the Western blotting method in the left ventricle. Results. …”
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Interleukin 10 Plays an Important Role in Neonatal Rats with Hypoxic-Ischemia Associated with B-Cell Lymphoma 2 and Endoplasmic Reticulum Protein 29 by Xue Bai, Liu-Lin Xiong, Chang-Le Fang, Hao-Li Zhou, Lu-Lu Xue, Yue Hu, Qing-Jie Xia, Jia Liu, Jun-Yan Zhang, Ting-Hua Wang, Si-Jin Yang

“…The expression of IL-10 in the cortex, hippocampus, and lung tissues was measured with immunohistochemistry, real-time quantitative polymerase chain reaction (RT-qPCR), and western blot (WB). Immunofluorescence double staining was performed to observe the localization of IL-10 in neurons and astrocytes. …”
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KAT2B inhibits proliferation and invasion via inactivating TGF-β/Smad3 pathway-medicated autophagy and EMT in epithelial ovarian cancer by Yuqin Yao, Yuna Niu, Honggui Zhou, Min Yong

“…RNA sequencing (RNA-seq) further identified altered gene expression profiles following KAT2B silencing in EOC cells. Western blot and qRT-PCR were employed to assess the protein and mRNA expression, respectively. …”
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Saharan Dust Events over the Valencian Community (Eastern Iberian Peninsula): Synoptic Circulation Patterns and Contribution to PM10 Levels by Ariadna Huerta-Viso, Javier Crespo, Nuria Galindo, Eduardo Yubero, Jose Francisco Nicolás

“…In Scenario 1 (17.4%), the transport of Saharan dust was due to the combination of a trough situated over the southwest of the Iberian Peninsula and a high-pressure system centered on western Algeria, Tunisia and eastern Libya. According to the PDF analysis, SDE characterized by this type of pattern were the most likely to substantially increase PM10 mass concentrations. …”
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