Regulation of Hypoxia-Induced Cell Death in Human Tenocytes by Min Liang, Hannah R. Cornell, Nasim Zargar Baboldashti, Mark S. Thompson, Andrew J. Carr, Philippa A. Hulley

“…Cultured human tenocytes were exposed to hypoxia and the cellular response detected using QPCR, Western blotting, viability, and ELISA assays. We find that tenocytes respond to hypoxia in vitro by activating classical HIF-1α-driven pathways. …”
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Challenges and strategies in meeting urban tree canopy targets: A scenario discovery analysis to guide urban forest planning in new developments by Raymundo Marcos Martinez, Natthanij Soonsawad, Mariana Dias Baptista, Stephen Cook, Guy Barnett, Joseph Chee, Tim Field, Tim Muster

“…This study explores strategies for achieving a 40 % tree canopy cover target in a new development within the Western Sydney Aerotropolis Precinct, NSW, Australia, using scenario discovery analysis. …”
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An Effective Phytoconstituent Aconitine: A Realistic Approach for the Treatment of Trigeminal Neuralgia by Dilek Çankal, Esra Küpeli Akkol, Yeliz Kılınç, Mert İlhan, Raffaele Capasso

“…Following the completion of tests, ipsilateral and contralateral spinal cords were harvested for Western blot analyses to assess NR-1 protein expression. …”
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Auriculotherapy Modulates Macrophage Polarization to Reduce Inflammatory Response in a Rat Model of Acne by Guang Zuo, Yidan Gao, Guangtong Lu, Ming Bu, Jun Liu, Juncha Zhang, Xisheng Fan, Hao Chen, Xuesong Wang, Yanfen She

“…The polarization of macrophages was analyzed by flow cytometry, and the expression of TLR2/NF-κB signaling pathway in the target tissues was analyzed using western blot. Results. ABT, APS, and ABPS all reduced the erythema of ear acne, decreased microcirculation in localized ear acne, and decreased serum levels of TNF-α and IL-1β in rats. …”
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The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons by Catherine Schaefers, Simone Rothmiller, Horst Thiermann, Theo Rein, Annette Schmidt

“…The identity of hiPSC, neuronal progenitors, and mature MNs was continuously verified by the detection of specific markers at the genetic and protein level via qRT-PCR, flow cytometry, Western Blot, and immunofluorescence. MNX1- and ChAT-positive motoneuronal progenitor cells were formed after neural induction via dual-SMAD inhibition and expansion. …”
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Efficacy and safety of acupuncture with moxibustion for knee osteoarthritis: a meta-analysis of randomized controlled trials by Rui-Xin Zhu, Xing Li, Zhao-Bo Yan, Cheng Chen, Wei Zhai, Heng-Cai Zhang, Wen-Juan Ma, Fu-Kun Wang, Mi Liu, Ge-Shu Du

“…Acupuncture combined with moxibustion demonstrated significant improvement in the visual analog scale (VAS) for pain and the long-term (> 60 weeks) Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) scores for pain, stiffness, and physical function compared to other treatments. …”
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Influence of large-scale atmospheric circulation and Mediterranean sea surface temperature to extreme land precipitation: the case of storm Alex by Laurent Terray, Margot Bador

“…We first show that the mean effect of the observed atmospheric circulation pattern, an intense low-pressure system centered over the coasts of Western Europe associated with a cold pool and a secondary low-pressure system, can explain about 80% of the precipitation event magnitude. …”
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Becoming smart “digital natives”: cultivating Chinese English majors’ new media literacy via Journalism English Reading and Listening by Zhang Danyang, Wu Yang

“…English majors are responsible for learning English languages and western cultures and serve as interlocutors between the East and the West. …”
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Study of RNA Interference Targeting NET-1 Combination with Sorafenib for Hepatocellular Carcinoma Therapy In Vitro and In Vivo by Song He, Ying-ze Wei, Gui-lan Wang, Yu-yin Xu, Jia-ming Zhou, Yi-xin Zhang, Li Chen

“…The expressions of NET-1 mRNA and protein were detected by RT-QPCR and western blot. The ability of proliferation was determined by CCK-8 assay. …”
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Barriers and Strategies to Lifestyle and Dietary Pattern Interventions for Prevention and Management of TYPE-2 Diabetes in Africa, Systematic Review by Hirut Bekele, Adisu Asefa, Bekalu Getachew, Abebe Muche Belete

“…The main barriers to adherence were both systemic (population changes, poor access, western cultural influences, and low-quality healthcare) and personal (poverty and cost, educational status, and perceptions about the disease) in nature. …”
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Mouse Umbilical Cord Mesenchymal Stem Cell Paracrine Alleviates Renal Fibrosis in Diabetic Nephropathy by Reducing Myofibroblast Transdifferentiation and Cell Proliferation and Upr... by Hongde Li, Pengfei Rong, Xiaoqian Ma, Wei Nie, Yan Chen, Juan Zhang, Qiong Dong, Min Yang, Wei Wang

“…Next, mesangial cells were exposed to 5.6 mM glucose, 30 mM glucose, or mUC-MSC-conditioned medium, and then we performed western blotting, immunofluorescence, wound healing assay, and cell proliferation assay to measure extracellular matrix (ECM) proteins and matrix metalloproteinases (MMPs), myofibroblast transdifferentiation (MFT), and cell proliferation. …”
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Trees, seeds and seedlings analyses in the process of obtaining a quality planting material for black locust (Robinia pseudoacacia L.) by Andrea M. ROMAN, Irina M. MORAR, Alina M. TRUTA, Catalina DAN, Adriana F. SESTRAS, Liviu HOLONEC, Florin IORAS, Radu E. SESTRAS

“…The objective of the hereby study was to identify a quality planting material at black locust using seeds from trees with superior traits from five stands geographically close, located in North-western of Romania. An empirical selection was done, thus trees with the most favourable traits were selected as plus trees. …”
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The functioning of the Crimean Tatar language in the authorities and administration of the Crimean Autonomous Soviet Socialist Republic in the context of the policy of indigenizati... by Grigorii Kondratjuk

“…The Bolsheviks’ hopes for a proletarian revolution in Western Europe did not come true, and the vector of foreign policy hopes became the East (Turkey and Iran). …”
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Human Urinary Kallidinogenase Reduces Lipopolysaccharide-Induced Neuroinflammation and Oxidative Stress in BV-2 Cells by Zhongyan Zhao, Zhiyu Xu, Tao Liu, Shixiong Huang, Huai Huang, Xiaoyun Huang

“…Expressions of tumor necrosis factor-α (TNFα), prostaglandin E2 (PGE2), interleukin-6 (IL-6), and interleukin-1β (IL-1β) were examined with the ELISA method and western blot. Intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) were measured to determine oxidative stress. …”
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Agro-Morphological Characterization of Kenyan Slender Leaf (Crotalaria brevidens and C. ochroleuca) Accessions by Fridah A. Mwakha, Bernard M. Gichimu, Johnstone O. Neondo, Peter K. Kamau, Eddy O. Odari, Joshua K. Muli, Nancy L. M. Budambula

“…Slender leaf (Crotalaria spp) is among the indigenous and underutilized vegetables in Kenya whose production is limited to the Western and Coastal regions of the country. For a long time, this crop has been neglected in terms of research and genetic improvement. …”
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Melatonin Reverses the Loss of Stemness Induced by TNF-α in Human Bone Marrow Mesenchymal Stem Cells through Upregulation of YAP Expression by Xudong Wang, Tongzhou Liang, Jincheng Qiu, Xianjian Qiu, Bo Gao, Wenjie Gao, Chengjie Lian, Taiqiu Chen, Yuanxin Zhu, Anjing Liang, Peiqiang Su, Yan Peng, Dongsheng Huang

“…The results of colony formation assays, Alizarin red staining, western blotting, and reverse transcription-polymerase chain reactions suggest that melatonin can reverse the inflammatory damage caused by TNF-α treatment in the third, seventh, and tenth generations of primary BMMSCs (vs. control and the TNF-α-treated group). …”
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Advances in antiviral strategies targeting mosquito-borne viruses: cellular, viral, and immune-related approaches by Ayyaz Khan, Zakirullah, Shahid Wahab, Seong-Tshool Hong

“…., Chikungunya, Eastern and Western Equine Encephalitis viruses), also transmitted by Aedes and Culex; Bunyaviridae (recently reorganized), containing viruses like Rift Valley Fever and Oropouche virus, transmitted by mosquitoes and sometimes sandflies; and Reoviridae, which includes the genus Orbivirus (e.g., West Nile and Bluetongue viruses), primarily affecting animals and transmitted by mosquitoes and sandflies. …”
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Genetic and biochemical analysis of severe hypertriglyceridemia complicated with acute pancreatitis or with low post-heparin lipoprotein lipase mass by Takashi Suzuki, Makoto Kurano, Akari Isono, Takuya Uchino, Yohei Sayama, Honami Tomomitsu, Daiki Mayumi, Ruriko Shibayama, Toru Sekiguchi, Naoki Edo, Kiyoko Uno-Eder, Kenji Uno, Koji Morita, Toshio Ishikawa, Kazuhisa Tsukamoto

“…Three patients were homozygous for APOA5 rs2075291, and one was heterozygous. ELISA and Western blot analysis of the serum revealed the existence of apolipoprotein A-V in the lipoprotein-free fraction regardless of the presence or absence of rs2075291; furthermore, the molecular weight of apolipoprotein A-V was different depending on the class of lipoprotein or lipoprotein-free fraction. …”
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The Impact of Modifiable Risk Factors on the Endothelial Cell Methylome and Cardiovascular Disease Development by Hashum Sum, Alison C. Brewer

“…Cardiovascular disease (CVD) is the most prevalent cause of mortality and morbidity in the Western world. A common underlying hallmark of CVD is the plaque-associated arterial thickening, termed atherosclerosis. …”
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What ‘unexplored’ means: mapping regions with digitized natural history records to look for ‘biodiversity blindspots’ by Laymon Ball, Sheila Rodríguez-Machado, Diego Paredes-Burneo, Samantha Rutledge, David A. Boyd, David Vander Pluym, Spenser Babb-Biernacki, Austin S. Chipps, Rafet Ç. Öztürk, Yahya Terzi, Prosanta Chakrabarty

“…., have relatively low, or no evidence of, past sampling efforts) under typical Western-scientific descriptions. We also question what ‘unexplored’ may mean in these contexts and explain how replacing the term in favor of more nuanced phrasing (e.g., ‘biodiversity blindspots,’ which emphasizes the lack of publicly available data about specimens) can mitigate future misunderstandings of natural history science. …”
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