Sodium Lactate Accelerates M2 Macrophage Polarization and Improves Cardiac Function after Myocardial Infarction in Mice by Jialiang Zhang, Fangyang Huang, Li Chen, Guoyong Li, Wenhua Lei, Jiahao Zhao, Yanbiao Liao, Yijian Li, Changming Li, Mao Chen

“…Signaling proteins were measured by Western blotting. Results. Sodium lactate treatment following MI improved cardiac performance, enhanced anti-inflammatory macrophage proportion, reduced cardiac myocytes apoptosis, and increased neovascularization. …”
Get full text

MicroRNA-32-5p promotes the proliferation and metastasis of gastric cancer cells by Chao Sun, Lai-gang Huang, Bing Leng, Yanting Guo, Chen Chen, Ruijie Lv, Yan Dong, Tian-tian Gao, De-qing Sun

“…Clonal formation, MTT, caspase-3 activity, sperm DNA fragmentation, flow cytometry assay, cell adhesion, transwell assays were performed to detect the functions of miR-32-5p or anti-miR-32-5p on the growth and metastasis of GC cells. Western blot, qRT-PCR, Co-immunoprecipitation, and luciferase reporter analysis were performed to explore the associated mechanisms. …”
Get full text

The Effect of Pulsatile Flow on bMSC-Derived Endothelial-Like Cells in a Small-Sized Artificial Vessel Made by 3-Dimensional Bioprinting by Kang Woog Lee, Dae-Hyun Kim, Jun Hee Lee, Young-Nam Youn

“…Endothelial differentiation was confirmed by the Matrigel tube formation assay, Western blot, and qRT-PCR. In addition, embedment of endothelial-like cells in an artificial vessel was made by 3-dimensional bioprinting, and the pulsatile flow was performed. …”
Get full text

Targeted Sanctions: a Tool of Foreign Policy, Unfair Competition or Global Social Engineering? by L. L. Fituni

“…Arguably, among those asymmetric and hybrid measures, Western countries assign a key role to the pressure of international sanction upon competitor nations and uncooperative actors on the world arena. …”
Get full text

A comparison of the parasitoid wasp species richness of tropical forest sites in Peru and Uganda – subfamily Rhyssinae (Hymenoptera: Ichneumonidae) by Hopkins, Tapani, Tuomisto, Hanna, Gómez, Isrrael C., Sääksjärvi, Ilari E.

“…During the past decades, we have carried out long-term Malaise trapping using a standardised approach in the tropical forests of Peru (western Amazonia) and Uganda (eastern Africa). Here, we test how well such data can be used for global comparisons, by comparing the results for the subfamily Rhyssinae (Hymenoptera: Ichneumonidae). …”
Get full text

The Roles of PI3K/AKT/mTOR and MAPK/ERK Signaling Pathways in Human Pheochromocytomas by Juan Du, Anli Tong, Fen Wang, Yunying Cui, Chunyan Li, Yushi Zhang, Zhaoli Yan

“…Protein phosphorylation was detected by Western blotting. Results. In most PPGLs, AKT, ERK1/2, and mTOR were activated. …”
Get full text

Fabrication of Core-Shell PEI/pBMP2-PLGA Electrospun Scaffold for Gene Delivery to Periodontal Ligament Stem Cells by Qiao Xie, Lie-ni Jia, Hong-yu Xu, Xiang-gang Hu, Wei Wang, Jun Jia

“…Moreover, the core-shell electrospun scaffold showed BMP2 expression for a much longer time (more than 28 days) compared with the single axial electrospun scaffold, as evaluated by qRT-PCR and western blot after culturing with hPDLSCs. These results suggested that the core-shell PEI/pBMP2-PLGA scaffold fabricated by coaxial electrospinning had a good gene release behavior and showed a prolonged expression time with a high transfection efficiency.…”
Get full text

Study of the chemical composition of argan oil according to the shape of the fruit by M. Hilali, L. El Hammari, H. El Monfalouti, N. Maata, B. E. Kartah

“…To investigate the links of argan fruit shape with the chemical composition of argan oil, with the help of native communities, 4 different fruit shapes (fusiform, apiculate, spherical and oval) were selected, which were harvested from the same place (Tamanar) in Essaouira province (South Plain region, Western Morocco). After harvesting the fruit of the argan tree, 100 samples were taken from each form. …”
Get full text

Modern Trends in Public Funding of Higher Education by M. De Martino, G. F. Tkach, S. A. Kovalenko

“…This paper discusses the main mechanisms of financing higher education systems and analyzes the structures and features of these mechanisms using the examples of the largest countries of Western Europe and Asia – Germany, Spain, Italy, France, and China.Materials and methods. …”
Get full text

New insight into the controlling factors of differences in carbonate buried-hills driven by tectonic activity: a case study of the Weixinan Sag, Beibuwan Basin, China by Yanying Li, Kongyou Wu, Jia Zhong, Yannan Du, Jinyou Wang

“…Abstract As the main field for crude oil exploration in the western of the South China Sea, the Weixinan Sag, Beibuwan Basin, has discovered multiple hydrocarbon-bearing structures. …”
Get full text

Multi-omics analysis reveals that neutrophil extracellular traps related gene TIMP1 promotes CRC progression and influences ferroptosis by Yuzhao Jin, Luyu Liao, Qianping Chen, Bufu Tang, Jin Jiang, Ji Zhu, Minghua Bai

“…We used small interfering RNA to knockdown TIMP1, and verified the ability of TIMP1 in CRC proliferation, invasion and migration through western blot, transwell, cell scratch assay, cell clone formation and other experiments. …”
Get full text

Assessment of Economic Management of Land Resources to Enhance Food Security by Dinara Tleshpayeva, Natalia Bondarenko, Mikhail Leontev, Galina Mashentseva, Julia Plaksa, Andrey Zharov, Diana Stepanova, Agzhan Karbozova

“…Based on document analysis and expert surveys, an optimal land ratio of approximately 30% meadows/pastures, 20% arable land, and 20% water is recommended, aligned with practices in Western Europe and North America. The study suggests measures for sustainable land use, including transferring degraded lands to pasture, preferential taxation for ecologically clean production, and financial incentives for restoring and conserving land. …”
Get full text

DNA Damage Sensor γ-H2AX Is Increased in Preneoplastic Lesions of Hepatocellular Carcinoma by Yasunobu Matsuda, Toshifumi Wakai, Masayuki Kubota, Mami Osawa, Masaaki Takamura, Satoshi Yamagiwa, Yutaka Aoyagi, Ayumi Sanpei, Shun Fujimaki

“…Appropriate cases were also examined by fluorescence analysis and western blotting. Results. All cases with chronic liver disease showed increased levels of γ-H2AX expression. …”
Get full text

Scutellarin Prevents Angiogenesis in Diabetic Retinopathy by Downregulating VEGF/ERK/FAK/Src Pathway Signaling by Lingli Long, Yubin Li, Shuang Yu, Xiang Li, Yue Hu, Tengfei Long, Liqin Wang, Wenwen Li, Xiaoxin Ye, Zunfu Ke, Haipeng Xiao

“…A streptozotocin-induced type II diabetic rat model was established to measure the effects of oral administration of SCU on protecting retinal microvascular dysfunction by Doppler waveforms and HE staining. We further used western blot, luciferase reporter assay, and immunofluorescence staining to study the antiangiogenic mechanism of SCU. …”
Get full text

Integrative transcriptomic and metabolomic reveals squalene alleviating the inflammatory response and enhancing immunity of hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lance... by Xiaobo Yan, Simiao Pan, Xiangxiang Suo, Weibin Huang, Tao Li, Shuang Zhang, Yuanzhi Yang, Beiping Tan, Xiaohui Dong

“…Transcriptomic and metabolomic analyses showed that squalene activated the Wnt/β-Catenin signaling pathway and PI3K-Akt signaling pathway. Western blot results showed that squalene ingestion significantly upregulated Wnt/β-Catenin protein expression and downregulated the Axin protein expression (P < 0.05). …”
Get full text

Assessing the influence of matcha green tea extract on NF-κB/MAPK signaling pathway activation in rats: A study on irradiation-induced acute hepatic inflammatory response by Noha Sayed Hamed, Eithar K. El Adham, Amal I. Hassan

“…Next, a series of biochemical experiments were conducted to examine the impact of M on lipid peroxidation levels, antioxidant enzyme activities, and pro-inflammatory cytokines (IL-6, IL-10, and TNF-α) in liver tissue. The Western blot method was employed for this analysis. …”
Get full text

Desertification risk assessment and management program by M. Akbari, M. Ownegh, H.R. Asgari, A. Sadoddin, H. Khosravi

“…The present study is aimed to assess the hazard and risk of desertification and to develop management programs in the semi-arid western regions of Golestan Province in Iran. Desertification rate was obtained using the Iranian model of desertification potential assessment. …”
Get full text

Comparison of Direct Runoff Hydrographs of Two Ungauged Sub-Basins Using Instantaneous Unit Hydrograph Approach by Hüseyin Akay, Müsteyde Baduna Koçyiğit

“…For the analysis, two neighboring sub-basins of Araç Creek located in the Western Black Sea Basin were selected. Drainage networks of the sub-basins were created via ArcGIS and some morphometric parameters were calculated. …”
Get full text

Knockdown of YAP/TAZ Inhibits the Migration and Invasion of Fibroblast Synovial Cells in Rheumatoid Arthritis by Regulating Autophagy by Wei Zhou, Qin Shen, Hui Wang, Jie Yang, Chen Zhang, Zijing Deng, Keyan Wu, Yang Zhou, Jing Zeng, Yu Zhang, Weigan Shen

“…Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) and western blotting assays were performed to examine the expression of indicated genes. …”
Get full text

Rapid Action of Aldosterone on Protein Levels of Sodium-Hydrogen Exchangers and Protein Kinase C Beta Isoforms in Rat Kidney by Somchit Eiam-Ong, Mookda Chaipipat, Krissanapong Manotham, Somchai Eiam-Ong

“…After 30 minutes, abundance and immunoreactivity of these proteins were determined by Western blot analysis and immunohistochemistry, respectively. …”
Get full text