Relaxin inhibits 177Lu-EDTMP associated cell death in osteosarcoma cells through notch-1 pathway by Xu Junhua, Wan Song, Chen Wei, Zhang Yi, Ji Zhenzhong

“…ELISA-based kit was used to determine apoptotic DNA fragmentation. Western blotting was used to determine expression levels of apoptotic-related signalling pathway proteins like bcl2, poly(ADP-ribose) polymerase (PARP), and MAPK (mitogen-activated protein kinase). …”
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Diabetes-Resistant NOR Mice Are More Severely Affected by Streptozotocin Compared to the Diabetes-Prone NOD Mice: Correlations with Liver and Kidney GLUT2 Expressions by S. Kahraman, C. Aydin, G. O. Elpek, E. Dirice, A. D. Sanlioglu

“…We also detected GLUT2 expression profiles in NOD and NOR hepatic and renal tissues by western blot analysis and observed considerably higher GLUT2 expression levels in liver and kidney tissues of NOR mice. …”
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Preliminary species diversity and community phylogenetics of wood-inhabiting basidiomycetous fungi in the Dabie Mountains, Central China reveal unexpected richness by Xiang-Yang Liu, Shi-Liang Liu, Hao-Wen Wei, Xue-Wei Wang, Jia Yu, Shan Shen, Li-Wei Zhou

“…The five sampling sites in the eastern part of the Dabie Mountains had significantly higher species diversity and phylogenetic diversity of wood-inhabiting basidiomycetous fungi than those in the western part, and thus deserve priority in terms of conservation. …”
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Protein C deficiency with recurrent systemic thrombosis associated with compound heterozygous PROC missense variants by Mikio Shiba, Shuichiro Higo, Yu Morishita, Yasuhiro Ichibori, Yoshihiro Kin, Yasushi Sakata, Yoshiharu Higuchi

“…PCR amplification of genomic DNA spanning these exons followed by Sanger sequencing analysis revealed that the c.76G>A and the synonymous c.423G>T variants were in the same allele, whereas the c.1000G>A variant was on the opposite allele, indicating compound heterozygosity. Western blot analysis of Huh-7 and HEK293T cells transfected with expression vectors encoding PROC with or without these variants demonstrated that Gly334Ser-PROC expression levels were significantly decreased in culture media collected from HEK293T cells, while the expression levels of protein C with these variants were not significantly altered in cell lysates. …”
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Research on the Pore Evolution of Sandstone in Cold Regions under Freeze-Thaw Weathering Cycles Based on NMR by Zheng Pan, Keping Zhou, Rugao Gao, Zhen Jiang, Chun Yang, Feng Gao

“…To study the mesoscopic evolution law of the rock pore structure under freeze-thaw weathering cycles, a freeze-thaw weathering cycle experiment was performed on red sandstone from the cold region of western China with temperatures ranging from -20°C to +20°C. …”
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Atractylenolide-I prevents abdominal aortic aneurysm formation through inhibiting inflammation by Shuxiao Chen, Xiaotian Liu, Xincheng Zhou, Weixiao Lin, Minting Liu, Haoran Ma, Keli Zhong, Qiming Ma, Chengjian Qin, Chengjian Qin

“…The pathological and molecular alteration was investigated by immunostaining, ELISA, qRT-PCR and Western blotting.ResultsATL-I inhibited the dilatation of the abdominal aorta and decreased the incidence of AAA. …”
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Expression of Sclerostin in Osteoporotic Fracture Patients Is Associated with DNA Methylation in the CpG Island of the SOST Gene by Yanming Cao, Bin Wang, Ding Wang, Dongxiang Zhan, Caiyuan Mai, Peng Wang, Qiushi Wei, Yamei Liu, Haibin Wang, Wei He, Liangliang Xu

“…After extracting total RNA and protein, we checked the relative expression levels of SOST by quantitative real-time PCR and western blot. Also, immunohistochemical staining was performed to observe the expression of SOST protein in the bone samples. …”
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Cobalt Protoporphyrin Downregulates Hyperglycemia-Induced Inflammation and Enhances Mitochondrial Respiration in Retinal Pigment Epithelial Cells by Peng-Hsiang Fang, Tzu-Yu Lin, Chiu-Chen Huang, Yung-Chang Lin, Cheng-Hung Lai, Bill Cheng

“…Protein expressions in samples were determined by either Western blotting or immunostaining. A Seahorse metabolic analyzer was used to assess the impact of CoPP treatment on mitochondrial respiration in ARPE-19 cells in NG or HG media. …”
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Legal Transplants, Legal Survivals, and Legal Revivals: Towards a Reconceptualisation of The Circulation of Legal Forms in Time and Space by Rafał Mańko

“…However, his notion of a legal transplant conflates two quite different realities: on the one hand, the borrowing of legal forms from other, simultaneously existing legal systems (such as the transplant of the Swiss Civil Code to Atatürk’s Turkey) and, on the other hand, the rediscovery of old legal forms and their “borrowing” from long defunct legal systems (such as the rediscovery of Justinian’s Corpus Iuris Civilis by medieval lawyers in Western Europe, and the infusion of Roman ideas about contract law into existing customary rules). …”
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NRBF2 plays a crucial role in the acquisition process of learning and memory, independent of the Vps34 complex by Songfen Wu, Songfen Wu, Songfen Wu, Haicai Zhuang, Haicai Zhuang, Haicai Zhuang, Xidan Zhou, Kuan Li, Kuan Li, Kuan Li

“…Employing quantitative PCR, Western blot analysis, behavioral tests, and electrophysiology, we investigated the mechanisms through which NRBF2 affects memory processing.ResultsWe observed an increase in Nrbf2 mRNA levels at 6 and 12 h, and protein levels at 6 h post fear conditioning. …”
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MSC-derived exosome ameliorates pulmonary fibrosis by modulating NOD 1/NLRP3-mediated epithelial-mesenchymal transition and inflammation by Wei Chen, Jie Peng, Xiangyi Tang, Shao Ouyang

“…Classic molecular biology approaches such as RT-qPCR, Western blot, immunofluorescence, and ELISA were used to examine molecular pathways. …”
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Autophagy-related long non-coding RNA MIR210HG plays a therapeutic role in hepatocellular carcinoma by Chaoqun Zhang, Dianxing Sun, Huifang Zhou, Chao Liu, Jie Ruan, Jiwen Kang, Ying Xie

“…Functional assays, including CCK-8, Transwell, flow cytometry, and western blot, were performed to evaluate the effects of lncRNA MIR210HG on cell proliferation, invasion, apoptosis, and autophagy in hepatocellular carcinoma. …”
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eDNA Metabarcoding Analysis of Bony Fish Identification in Coastal Water of Gulf of Maine Using Nested Polymerase Chain Reaction of 12S rRNA Universal Primers by Bo‐Young Lee, Grant A. Milne, Corwin Freedman, Jenifer Miksis‐Olds, Bonnie L. Brown

“…To our knowledge, this is the first metabarcoding study to assess fish communities in the Western Atlantic region using the MiFish primer set, and the study suggests that metabarcoding is useful for mapping geographic and temporal marine fish diversity.…”
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Spatial Variations in PAHs and Nitro-PAHs Bound to Total Suspended Solids in Urban Locations in Taiwan by Hsin-Chieh Kung, Bo-Wun Huang, Justus Kavita Mutuku, Guo-Ping Chang-Chien

“…Abstract The concentration of 16 PAHs and 13 nitro-PAHs bound to total suspended particles were evaluated in outdoor environments in three cluster study locations in the western side of Taiwan. Samples were collected based on U.S. …”
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New biomarkers for diagnosis of bladder cancer: a bibliometric analysis by Roberto Falabella, Valentina De Simone, Felice Crocetto, Francesco Del Giudice, Angelo Porreca, Nazario Foschi, Biagio Barone, Luca Di Gianfrancesco, Valentina Di Pasquale, Vincenzo Francesco Caputo

“… Background/Objectives: Bladder cancer is a multifactorial disease, ranking as the 10th most common cancer globally and the fourth most common cancer in men and the ninth in women in the Western world. This bibliometric analysis aims to identify and evaluate scientific literature addressing new biomarkers for bladder cancer diagnosis, as well as to identify the most prolific organizations, authors, journals, countries, and keywords within this research domain. …”
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Impact of the Luding Earthquake on the Area of Potentially Suitable Habitat for <i>Batrachuperus</i> Species in the Gongga Mountain National Nature Reserve by Xinlong Song, Xiuying Liu, Xiaoao Zheng, Jian Song, Zhangqiang You, Jianli Xiong

“…Currently, suitable habitats accounted for 40.67% (1664.10 km²) of the studied area (4091.10 km²), and these areas were mainly distributed in the northern, western, and southern portions of the GGMNNR. The destruction caused by the earthquake accounted for 4.87% (199.34 km²) of the studied area, and only 1.97% (32.78 km²) of the suitable habitat of the <i>Batrachuperus</i> species was destroyed. …”
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Adipose-Derived Mesenchymal Stem Cell Exosomes Suppress Hepatocellular Carcinoma Growth in a Rat Model: Apparent Diffusion Coefficient, Natural Killer T-Cell Responses, and Histopa... by Sheung-Fat Ko, Hon-Kan Yip, Yen-Yi Zhen, Chen-Chang Lee, Chia-Chang Lee, Chung-Cheng Huang, Shu-Hang Ng, Jui-Wei Lin

“…ADMSC-derived exosomes appeared as nanoparticles (30–90 nm) on electron microscopy and were positive for CD63, tumor susceptibility gene-101, and β-catenin on western blotting. The control (n=8) and exosome-treated (n=8) rats with N1S1-induced HCC underwent baseline and posttreatment day 10 and day 20 magnetic resonance imaging and measurement of ADC. …”
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How physical activity opportunities seized by adolescents differ between Europe and the Pacific Islands: the example of France and New Caledonia [version 1; peer review: 2 approved... by Olivier Galy, Guillaume Wattelez, Francois Potdevin, Marie-Jeanne Urvoy, Thibaut Derigny, Paul Zongo, Pierre-Yves Leroux, Christophe Schnitzler

“…Background France (FR) and New Caledonia (NC) are both French territories, one in Western Europe, the other as part of the Pacific Island Countries and Territories (PICTs). …”
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Accumulation of advanced oxidative protein products exacerbate satellite glial cells activation and neuropathic pain by Chen Tu, Shi-Cheng Wang, Meng-Xuan Dai, Si-Qi Lai, Zhi-Wei Huang, Yong-Peng Yu, Yun-Biao Chen, Ji-Huan Zeng, Liang Wang, Zhao-Ming Zhong

“…The effects of AOPPs on the activation of satellite glial cells (SGCs) in the dorsal root ganglion (DRG), receptor for advanced glycation end-products (RAGE) expression, and NF-κB signaling pathway activation were also investigated using western blotting, immunofluorescence, and the Fluo4-AM fluorescence probe for calcium signaling. …”
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Spatial-temporal characteristics of agricultural economic resilience and spatial spillover effects of driving factors: evidence from provincial panel data in China by Xiaoyu Chen, Haohan Wang, Xingyuan Zhu, Xiao Zhang

“…Furthermore, AER exhibits a strong positive spatial correlation, with higher concentrations in the eastern and central regions, while the northeastern and western areas show lower levels. Key factors such as market scale, agricultural GDP, agricultural inputs, research and development, environmental conditions, and urbanization all shape AER. …”
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