Mahuang Fuzi Xixin Decoction Ameliorates Allergic Rhinitis in Rats by Regulating the Gut Microbiota and Th17/Treg Balance by Xiao Liang, Chang-Shun Liu, Xiao-Han Wei, Ting Xia, Fei-Long Chen, Qing-Fa Tang, Meng-Yue Ren, Xiao-Mei Tan

“…Inflammatory and immunological responses were assessed by histopathology, ELISA, flow cytometry, and western blotting. Our study demonstrated that MFXD reduced the symptom scores of AR and serum IgE and histamine levels. …”
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Neural Differentiation of Human Adipose Tissue-Derived Stem Cells Involves Activation of the Wnt5a/JNK Signalling by Sujeong Jang, Jong-Seong Park, Han-Seong Jeong

“…Interestingly, Wnt5a expression was highly increased in NI-hADSCs by real time RT-PCR analysis and western blot. Wnt5a level was upregulated after neural differentiation and Wnt3, Dvl2, and Naked1 levels were downregulated. …”
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The ERK1/2 Inhibitor U0126 Attenuates Diabetes-Induced Upregulation of MMP-9 and Biomarkers of Inflammation in the Retina by Ghulam Mohammad, Mohammad Mairaj Siddiquei, Mohammad Imtiaz Nawaz, Ahmed M. Abu El-Asrar

“…The expression of inducible nitric oxide synthase (iNOS), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) was examined by Western blot analysis. MMP-9 expression was significantly higher in diabetic rat retinas compared to controls at all time points.TIMP-1 expression was nonsignificantly upregulated at 1week of diabetes and was significantly downregulated at 4 and 12 weeks of diabetes. …”
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Sirtuin-2 Protects Neural Cells from Oxidative Stress and Is Elevated in Neurodegeneration by Preeti Singh, Peter S. Hanson, Christopher M. Morris

“…SIRT2 in human tissues was evaluated using Western blotting, immunohistochemistry, and fluorometric activity assays. …”
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LncRNA CASC15 upregulates cyclin D1 by downregulating miR-365 in laryngeal squamous cell carcinoma to promote cell proliferation by Minhui Zhu, Caiyun Zhang, Peng Zhou, Shicai Chen, Hongliang Zheng

“…The expression levels of proteins and mRNAs were determined by Western blotting analysis and qRT-PCRs, respectively. …”
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Small Interfering RNA Targeted to ASPP2 Promotes Progression of Experimental Proliferative Vitreoretinopathy by Xiao-Li Chen, Yu-Jing Bai, Qin-Rui Hu, Shan-Shan Li, Lv-Zhen Huang, Xiao-Xin Li

“…EMT markers and related inflammatory and fibrosis cytokines were measured by western blot or flow cytometry. Additionally, PVR rat models were induced by intravitreal injection of ARPE-19 cells transfected with ASPP2-siRNA and evaluated accordingly. …”
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circSPECC1 Promotes Proliferation and Migration of LNCaP Prostate Cancer Cells by Affecting Their Epithelial-Mesenchymal Transition by Fangmei Xie, Jian Shen, Zeping Han, Wenfeng Luo, Liyin Liao, Jinhua He

“…MTT and clonogenic assays were used to assess proliferative ability while a scratch test was used to analyze cell migration. Western blotting was used to determine protein expression levels. …”
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Long-Term Trends in Marriage Timing and the Impact of Migration, the Netherlands (1650-1899) by Charlotte Störmer, Corry Gellatly, Anita Boele, Tine De Moor

“…A closer look at regional variation reveals clear differences between the provinces and between urban and rural settings with those in the western part of the country and in urban centers marrying earlier. …”
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Analyzing Drought Propagation and Its Influential Factors in the Upper Nan Watershed, Thailand by Muhammad Chrisna Satriagasa, Piyapong Tongdeenok, Naruemol Kaewjampa

“…The eastern UNW (Zone I) has the longest DPT, while the western UNW (Zone III) has the shortest. This study is distinguished by its integration of global teleconnection factors, such as El Niño-Southern Oscillation (ENSO), the Dipole Mode Index (DMI), and Pacific Decadal Oscillation (PDO), alongside local factors like climate, slope, and watershed morphometry. …”
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Z-Guggulsterone Induces Apoptosis in Gastric Cancer Cells through the Intrinsic Mitochondria-Dependent Pathway by Ruxi Lv, Min Zhu, Kun Chen, Haitao Xie, Hongxia Bai, Qingfa Chen

“…Flow cytometry and Hoechst staining were used to assess cell apoptosis. Western blotting was applied to evaluate FXR, small heterodimer partner (SHP), Bcl-2, and Bax protein expression. …”
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Alternative dispute resolution: Mediation as a model [version 2; peer review: 2 approved] by Naser Sherman, Bashar Talal Momani

“…Additionally, it anticipates the future effectiveness of mediation in jurisdictions lacking comprehensive legislation, drawing from successful Western experiences to guide potential developments in Arab legal frameworks.…”
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Expression of Glutathione Peroxidase and Glutathione Reductase and Level of Free Radical Processes under Toxic Hepatitis in Rats by Igor Y. Iskusnykh, Tatyana N. Popova, Aleksander A. Agarkov, Miguel Â. A. Pinheiro de Carvalho, Stanislav G. Rjevskiy

“…The analyses of Gpx1 and Gsr expression revealed that the transcript levels increased in 2.5- and 3.0-folds, respectively. Western blot analysis revealed that the amounts of hepatic Gpx1 and Gsr proteins increased considerably after CCl4 administration. …”
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MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wnt/β-Cateninsignaling Pathway by Xiaohong Wu, Yong Zhang, Junping Guo, Xun Yan, Li Shen, Jianping Zhou, Jun Zhao, Min Zhuang, Zhihong Cao

“…Also, related protein expressions on Wnt/β-catenin signaling pathway were analyzed by Western blot method. The expression of MAC30 was abnormally elevated in gastric cancer tissues, while interfering of its expression could significantly inhibit the proliferation of gastric cancer BGC-823 cell line. …”
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Oxidized DJ-1 Levels in Urine Samples as a Putative Biomarker for Parkinson’s Disease by Jihoon Jang, Soyeon Jeong, Sung Ik Lee, Wongi Seol, Hyemyung Seo, Ilhong Son, Dong Hwan Ho

“…In this study, we established an ELISA for OxiDJ-1 using recombinant DJ-1 treated with H2O2. Using Western blot assay and ELISA, we confirmed an increase of OxiDJ-1 from HEK293T cells treated with H2O2. …”
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Sirolimus Enhances Cyclosporine A-Induced Cytotoxicity in Human Renal Glomerular Mesangial Cells by Séin O'Connell, Craig Slattery, Michael P. Ryan, Tara McMorrow

“…Therefore investigations into the differential effects of these agents may identify potential mechanisms of nephrotoxicity and means to prevent ESRD induced by therapeutic drugs. Using ELISA, Western blotting, quantitative PCR and a reporter gene assay we detailed the differential effects of CsA and SRL in human renal mesangial cells. …”
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Measuring Chinese negative emotion towards climate change: Psychometric properties of the Chinese version of Climate Change Anxiety Scale by Sam SS Lau, Kubi Appiah, Cherry CY Ho, Marco CH Cheng, Bo-Yi Yang

“…Introduction: In response to growing concerns over negative emotions towards climate change worldwide, it is essential that a validated and reliable scale is available for the generalizability of the constructs being measured, and it is not just applicable to Western context, but also the East. Method: The present study aims to conduct a psychometric investigation of the 13-item Climate Change Anxiety Scale (CCAS) of Clayton and Karazsia in Traditional Chinese using a large sample (N = 1,567) of Chinese individuals in Hong Kong, China. …”
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Oral Immunotherapy Using Probiotic Ice Cream Containing Recombinant Food-Grade Lactococcus lactis Which Inhibited Allergic Responses in a BALB/c Mouse Model by Alireza Vasiee, Fereshteh Falah, Mojtaba Sankian, Farideh Tabatabaei-Yazdi, Seyed Ali Mortazavi

“…The cell extract of this strain was used for assessing the expression of the recombinant allergen in western blot analysis, and the existence of this protein with a molecular weight of 14.2 kDa was confirmed. …”
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Overexpression of metalloproteinase PAPPA accelerates cancer progression and correlates with immune cell infiltration in gastric cancer: insights from bioinformatics and in vitro i... by Shiya Yu, Shiyao Zheng, Jinwei Qiu, Hongming Lin, Xiaojuan Yu, Huiyan Chen, Junhong Wu, Weihang Wu, Jiong Chen, Yongyuan Chen, Jianwei Chen, Hu Zhao, Yu Wang

“…Cellular assays, including CCK-8, Western blotting, and flow cytometry, were utilized to examine PAPPA's role in gastric cancer cell apoptosis, migration, and invasion. …”
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Long-Term Statistical Analysis of Severe Weather and Climate Events in Greece by Vassiliki Kotroni, Antonis Bezes, Stavros Dafis, Dimitra Founda, Elisavet Galanaki, Christos Giannaros, Theodore Giannaros, Athanasios Karagiannidis, Ioannis Koletsis, George Kyros, Konstantinos Lagouvardos, Katerina Papagiannaki, Georgios Papavasileiou

“…The main results show that between 2010 and 2023, Greece experienced: nearly one heatwave per summer; heavy rainfall events, most common in winter and autumn, showing a significant increase, particularly in the eastern Aegean and western continental Greece; dry spells, which are longest in southern Greece; thunderstorm and hail events peaking in spring and summer; fire weather conditions and risk peaking in southern Greece. …”
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Monthly or Weekly Supplementation with Cholecalciferol 20,000 IU in People Living with HIV: Results from a Nested Cohort Study by Sebastian Noe, Christine I. Moeckel, Christiane Schwerdtfeger, Celia Oldenbuettel, Hans Jaeger, Eva Wolf, Christoph D. Spinner

“…We aimed to compare the efficacy of weekly versus monthly supplementation with cholecalciferol 20,000 IU in a group of PLWH with vitamin D deficiency in Western Europe. Study Design. Longitudinal, retrospective nested cohort study of PLWH from two large clinical care centers in Munich, Germany. …”
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