FHL1 as a prognostic biomarker and therapeutic target in acute promyelocytic leukaemia by Bo Luo, Wei Li, Jingyuan Zeng, Yingyu Mao, Shuang He, Nan Hu, Qulian Guo, Xiaoli Zheng

“…Quantitative real-time PCR and Western blot were used to assess the effects of small interfering RNA transfection and lentiviral interference on the gene's knockout and overexpression, respectively. …”
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DPSCs Protect Architectural Integrity and Alleviate Intervertebral Disc Degeneration by Regulating Nucleus Pulposus Immune Status by Xiwen Dong, Fanqi Hu, Jing Yi, Yuning Zhang, Chao Liu, Panpan Geng, Han Duan, Chu-Tse Wu, Xuesong Zhang, Hua Wang

“…To understand the biological effects of dental pulp stem cells (DPSCs) on nucleus pulposus (NP) cells, we carried out RNA sequencing, bioinformatic analysis which unveiled gene expression differences, and pathway variation in primarily isolated patients’ NP cells after treatment with DPSCs supernatant. Western blot and immunofluorescence were used to verify these molecular alterations. …”
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Anattempt to rebuild the world "in the American way" by A. D. Bogaturov

“…The US divides Europe into three parts; Western Europe, Eastern Europe, and Russia with Belarus. …”
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The Association between Female Reproductive Factors and Open-Angle Glaucoma in Korean Women: The Korean National Health and Nutrition Examination Survey V by Yong Un Shin, Eun Hee Hong, Min Ho Kang, Heeyoon Cho, Mincheol Seong

“…Only early menopause was associated with an increased risk of OAG in our study, in contrast to previous Western studies reporting both early menopause and late menarche as associated factors.…”
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Identification and Characterization of TEX101 in Bovine Epididymal Spermatozoa by Subir K. Nagdas, Eric L. McLean, Leeá P. Richardson, Samir Raychoudhury

“…Proteomic identification yielded ten peptides that matched the sequence of Testis Expressed 101 protein (TEX101). Western blots data revealed that bovine sperm TEX101 is present in both testicular and epididymal sperm PM fractions. …”
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Lignans from Patrinia scabiosaefolia improve insulin resistance by activating PI-3K/AKT pathway and promoting GLUT4 expression by Zhenhua Liu, Mengke Wang, Lijun Meng, Yixiao Chen, Qiuyi Wang, Yan Zhang, Xuefeng Xi, Wenyi Kang

“…While compound 4 only promoted glucose uptake by IR 3T3-L1 cells at 100 µmol/L (P < 0.01). Western blotting experiments showed that compounds 2 and 4 up-regulated the protein expressions of p-IRS, PI-3K, p-AKT and glucose transporter 4 (GLUT4), and promoted the transcription of GLUT4 mRNA. …”
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LAMB1 downregulation suppresses glioma progression by inhibiting aerobic glycolysis through regulation of the NF-κB/HK2 axis by Zhenxiang Zhao, Haiying Liu, Yingzi Liu, Junpeng Wen, Jiangwei Yuan

“…The EC50 of temozolomide (TMZ) in glioma cells was tested. Western blotting was conducted to monitor the expression of HK1, HK2, PDHA, and PKM. …”
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Th17: A New Participant in Gut Dysfunction in Mice Infected with Trichinella spiralis by Yu Fu, Wenfeng Wang, Jingjing Tong, Qi Pan, Yanqing Long, Wei Qian, Xiaohua Hou

“…The levels of IL-17, IL-23, and TGF-β1 in jejunum were measured by Western blot. Our results showed that the inflammation in jejunum was severe at 2 weeks postinfection (PI), which was not discernible at 8 weeks PI. …”
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Freshwater sponges in the southeastern U.S. harbor unique microbiomes that are influenced by host and environmental factors by Jacqueline G. Keleher, Taylor A. Strope, Noah E. Estrada, Allison M. Griggs Mathis, Cole G. Easson, Cara Fiore

“…Here we analyzed the microbiomes of three freshwater sponge species: Radiospongilla crateriformis, Eunapius fragilis, and Trochospongilla horrida, across four sites in western North Carolina, U.S.A. Our results support recent work indicating that freshwater sponges indeed harbor a distinct microbiome composition compared to the surrounding water and that these varied across sampling site indicating both environmental and host factors in shaping this distinct community. …”
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«Вот скажем лицом я в Китае». Стереотипы и имаготипы Китая, китайца и китайского в практике обращения к чужому в геопоэтике и геоэстетике Дмитрия Пригова... by Rainer Grübel

“…These phenomena and their historical development are of special interest in the present context of the Russian war against Ukraine, the Western sanctions against Russia and the growing political, economic and military approximation of Russia to China. …”
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MAGI2-AS3 hypermethylated in promoter region promotes migration and invasion of head and neck squamous cell carcinoma via miRNA-31-5p/AR axis by Kai Yue, Ting Zhang, Huanhuan Wang, Bo Wang, Yalin Mu, Hui Li

“…Molecular regulatory mechanism of MAGI2-AS3 in HNSCC is not yet mature.In this study, we analyzed the methylation level of MAGI2-AS3 promoter and its downstream miR-31-5p/AR axis by bioinformatics methods. qRT-PCR was used to detect the mRNA expression level of each gene, and western blot was used to detect the expression level of AR proteins in tissues and cells. …”
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NATIONAL ROMANTICISM IN WALT WHITMAN POEMS by Tomi Arianto

“…Thus, the representation of romantic ideas originating from Western Europe of the 18th century has penetrated into the 19th century America which is reflected in the works that carried Whitman. …”
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Sargassum plagiophyllum Ethanolic Extract Enhances Wound Healing by Modulating FAK/Src/Akt/p38 and Rac1 Signaling in Keratinocytes HaCaT Cells by Furoida Moolsup, Wanida Sukketsiri, Wipawadee Sianglum, Jirawat Saetan, Nattakanwadee Khumpirapang, Supita Tanasawet

“…Treatment with SPEE (50 and 100 μg/mL) increased FAK and Src phosphorylation in western blotting. Moreover, SPEE also upregulated Akt and p38 MAPK phosphorylation but not ERK1/2. …”
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Cathepsin C Is Involved in Macrophage M1 Polarization via p38/MAPK Pathway in Sudden Cardiac Death by Jialin Dai, Jiangjin Liu, Qiong Zhang, Yang An, Bing Xia, Changwu Wan, Yuanyuan Zhang, Yanni Yu, Jie Wang

“…This included a total of 265 differential proteins which were involved in SCD-related processes, such as inflammation, muscle system process regulation, metal ion transport, and lysosomal pathway. Western blotting was carried out to measure the expressions of cathepsin C (CTSC), focal adhesion kinase (FAK), p-FAK, and proteins related to the p38/MAPK signaling pathway, whereas immunohistochemistry was performed to determine the localization and expression of CTSC, TNF-α, and CD206 in arterial tissues. …”
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The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats by Yusu Ni, Diyan Chen, Yi Jiang, Danhong Qiu, Wen Li, Huawei Li

“…Expression of Shh (sonic hedgehog) and growth-associated protein 43 (GAP43, a neuronal marker) was detected in bilateral facial nuclei using reverse transcriptase PCR, western blotting analysis, and immunohistochemistry. …”
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Ginkgo biloba Extract Improves Insulin Signaling and Attenuates Inflammation in Retroperitoneal Adipose Tissue Depot of Obese Rats by Bruna Kelly Sousa Hirata, Renata Mancini Banin, Ana Paula Segantine Dornellas, Iracema Senna de Andrade, Juliane Costa Silva Zemdegs, Luciana Chagas Caperuto, Lila Missae Oyama, Eliane Beraldi Ribeiro, Monica Marques Telles

“…Rats were then euthanized and samples from retroperitoneal fat depot were used for western blotting, RT-PCR, and ELISA experiments. The GbE treatment promoted a significant reduction on both food/energy intake and body weight gain in comparison to the nontreated obese rats. …”
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Machine learning potential predictor of idiopathic pulmonary fibrosis by Chenchun Ding, Quan Liao, Renjie Zuo, Shichao Zhang, Zhenzhen Guo, Junjie He, Ziwei Ye, Weibin Chen, Sunkui Ke

“…Finally, the roles of PODNL1 and PIGA as biomarkers were validated through in vivo and in vitro experiments using qRT-PCR, Western blotting, and immunohistochemistry.ResultsThese findings suggest that PODNL1 and PIGA may serve as critical biomarkers for IPF onset and contribute to its pathogenesis.DiscussionThis study highlights their potential for early biomarker discovery and risk prediction in IPF, offering insights into disease mechanisms and diagnostic strategies.…”
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Capsule Endoscopy for Crohn’s Disease: Current Status of Diagnosis and Management by Dong-Hoon Yang, Bora Keum, Yoon Tae Jeen

“…Crohn’s disease (CD) is an idiopathic inflammatory bowel disease involving the small and/or large intestine. More than 50% of Western CD patients and up to 88% of Asian CD patients may have small intestinal involvement. …”
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Rat Nasal Respiratory Mucosa-Derived Ectomesenchymal Stem Cells Differentiate into Schwann-Like Cells Promoting the Differentiation of PC12 Cells and Forming Myelin In Vitro by Jian Zhang, Xin Gao, Hongjun Zou, Jinbo Liu, Zhijian Zhang

“…Immunocytochemical staining and Western blotting indicated that SC-like cells expressed the glial markers (GFAP, S100β, Galc, and P75) and CNPase. …”
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Autologous precision-cut lung slice co-culture models for studying macrophage-driven fibrosis by So-Yi Chang, So-Yi Chang, Wen-Hsin Chang, Wen-Hsin Chang, David C. Yang, Qi-Sheng Hong, Qi-Sheng Hong, Ssu-Wei Hsu, Ssu-Wei Hsu, Reen Wu, Ching-Hsien Chen, Ching-Hsien Chen

“…Fibrotic responses to nicotine, cigarette smoke extract (CSE), and a fibrosis-inducing cocktail (FC) were first evaluated in PCLS containing only tissue-resident macrophages, with upregulation of α-SMA-expressing fibroblasts confirmed by immunofluorescence and Western blotting, and collagen deposition quantified using Sirius Red staining. …”
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