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3701
Vitamin D Ameliorates Impaired Wound Healing in Streptozotocin-Induced Diabetic Mice by Suppressing Endoplasmic Reticulum Stress
Published 2018-01-01“…Endogenous markers of ER stress were quantified using Western blot and a real-time polymerase chain reaction. …”
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3702
Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901
Published 2016-01-01“…Thus, to seek for the crucial miRNA and key regulatory genes that establish ESC properties in MEK/ERK pathway, we performed microarray analysis and small RNA deep-sequencing of J1 mESCs treated with or without PD0325901 (PD), a well-known inhibitor of MEK/ERK signal pathway, followed by verification of western blot analysis and quantitative real-time PCR verification; we found that PD regulated the transcript expressions related to self-renewal and differentiation and antagonized the action of retinoic acid- (RA-) induced differentiation. …”
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3703
Nuclear Factor Erythroid 2-Related Factor 2 Intervenes the Release of Neutrophil Extracellular Traps during Lipopolysaccharide-Induced Acute Lung Injury in Mice
Published 2024-01-01“…Results showed that the levels of Cit-H3 measured by western blot in Nrf2-knockout (KO) mice were higher compared with the WT mice after LPS stimulation. …”
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3704
15-Lipoxygenase-1 Is Involved in the Effects of Atorvastatin on Endothelial Dysfunction
Published 2016-01-01“…Immunohistochemistry and western blotting showed that atorvastatin increased the expression of cellular adhesion molecules (CAMs) but decreased the expression of endothelial nitric oxide synthase (eNOS); these effects of atorvastatin were blocked by ALOX15 overexpression. …”
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3705
Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells
Published 2014-01-01“…The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohistochemistry using monoclonal antibodies DO-7, Pab240, and polyclonal antiserum CM-1. …”
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3706
Anti-cancer activity of synthetic gefitinib-1,2,3-triazole derivatives against Hela cells via induction of apoptosis
Published 2025-01-01“…Further investigations demonstrated that compound c13 triggered cell apoptosis and arrested the cell cycle at the G2/M phase in Hela cells. In addition, western blot analysis revealed that compound c13 upregulated the Bax/Bcl-2 ratio, and increased the levels of active caspase 3 and PARP1 cleavage, which suggested the involvement of the mitochondrial pathway in compound c13-induced apoptosis. …”
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3707
Preserved egg white alleviates DSS-induced colitis in mice through the reduction of oxidative stress, modulation of infl ammatory cytokines, NF-κB, MAPK and gut microbiota composit...
Published 2023-01-01“…The results showed that treatment with PEW in mice with DSS-induced colitis for 14 days effectively improved the clinical signs, inhibited the secretion and gene expression of pro-inflammatory cytokines, and reduced myeloperoxidase (MPO) activity and oxidative stress levels. In addition, western blotting results showed that PEW significantly suppressed DSS-induced phosphorylation levels of nuclear factor-kappa B (NF-κB) p65 and p38 mitogen-activated protein kinase (MAPK) in colon tissues of mice with colitis. …”
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3708
Polypeptide Globular Adiponectin Ameliorates Hypoxia/Reoxygenation-Induced Cardiomyocyte Injury by Inhibiting Both Apoptosis and Necroptosis
Published 2021-01-01“…Immunofluorescence staining and Western blotting demonstrated that both necroptosis and apoptosis were triggered by hypoxia/reoxygenation and diminished by globular adiponectin. …”
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3709
Pretreatment of Small-for-Size Grafts In Vivo by γ-Aminobutyric Acid Receptor Regulation against Oxidative Stress-Induced Injury in Rat Split Orthotopic Liver Transplantation
Published 2013-01-01“…Immunohistological assessment for apoptotic induction and western blotting for 4-hydroxynonenal, ataxia-telangiectasia mutated kinase (ATM), histone H2AX, phosphatidylinositol-3 kinase (PI3K), Akt, and free radical scavenging enzymes were performed. …”
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3710
THE EFFECTIVENESS OF ACUPUNCTURE ON ORTHOPAEDIC PAIN WITHIN A VOCATIONAL REHABILITATION SETTING
Published 2022-03-01“…Background: Acupuncture is rapidly rising in popularity within western populations since its development and consequently there is increasing interest from a variety of clients. …”
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3711
Spinal Involvement of TRPV1 and PI3K/AKT/mTOR Pathway During Chronic Postoperative Pain in Mice
Published 2025-01-01“…TRPV1 and PI3K/AKT/mTOR protein levels were evaluated by Western blotting. Results: The results showed that CPOP increased TRPV1 and mTOR protein levels, and pretreatment with the specific inhibitors alleviated CPOP. …”
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3712
Liberal Way of Life as a Political Philosophical Problem: Review of the Book: Lefebvre, A. (2024). Liberalism as a Way of Life. Princeton; Oxford: Princeton University Press, 285 p...
Published 2024-12-01“…It is no coincidence that the book by Alexandr Lefebvre, professor of political science and philosophy at the University of Sydney, has caused a rather complex range of impressions in the Western academic world and political journalism immediately after its publication by Princeton University Press. …”
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3713
ASPM Promotes the Progression of Anaplastic Thyroid Carcinomas by Regulating the Wnt/β-Catenin Signaling Pathway
Published 2022-01-01“…The data from two data sets (GSE76039 and GSE33630) were extracted and analyzed for the expression of ASPM, followed by a further validation in collected ATC patients using quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting. The effect of ASPM on cell proliferation, migration, invasion, and cell cycle was explored in ATC cell lines by in vitro inhibition of ASPM, while ASPM-mediated tumorigenicity was investigated in a xenograft tumor model. …”
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3714
PCSK9 Expression in Epicardial Adipose Tissue: Molecular Association with Local Tissue Inflammation
Published 2020-01-01“…The PCSK9 protein levels were measured by Western Blot in EAT and ELISA in plasma. PCSK9 was expressed at both the gene and protein levels in EAT. …”
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3715
Aquaporin 12 Is Expressed in the Stomach and Liver of the Spiny Dogfish (<i>Squalus acanthias</i>)
Published 2025-01-01“…A polyclonal antibody was made using a peptide from the derived amino acid sequence. Western blotting with the antibody showed faint banding around the size expected (33 kDa) by the 300 amino acid protein. …”
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3716
Little-Known Operations of the Soviet Troops in Liberation of Czechoslovakia
Published 2015-04-01“…In March - April 1945 as a result of the fighting of the 2nd and 4th Ukrainian fronts and friendly Czechoslovak and Romanian forces large groups of the Wehrmacht were defeated in the Western Carpathians. Slovakia and Moravia were completely liberated, including such major administrative and industrial centers such as Prague, Brno and Moravian Ostrava. …”
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3717
Corps (é)conduits : La construction d’un regard-machine dans Crash (1996) et Cosmopolis (2012) de David Cronenberg
Published 2023-06-01“…Attachment, dependency and eroticism as represented here are but the outcome of the way film history has given an increasingly complex and central focus to the car, paralleling its ever-growing fetishization within Western culture. When narrating the quest for the perfect car-crash in a group of fin de siècle fetishists or the physical and mental dissolution of a trader locked in his limousine, Cronenberg always makes the car become more than a prop or a means of transportation. …”
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3718
Syringaldehyde Alleviates Cardiac Hypertrophy Induced by Hyperglycemia in H9c2 Cells Through GLP-1 Receptor Signals
Published 2025-01-01“…Cells were treated with varying SA concentrations, and hypertrophic biomarkers were analyzed using ELISA, qPCR, and Western blot. <b>Results:</b> SA reduced cell size and hypertrophic biomarkers in a dose-dependent manner while increasing GLP-1 receptor expression and cAMP levels. …”
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3719
Comparative Study of Protein Expression Levels of Five Plaque Biomarkers and Relation with Carotid Plaque Type Classification in Patients after Carotid Endarterectomy
Published 2018-01-01“…Here, protein expression levels of the metalloproteases MMP-1, MMP-9, osteopontin (OPN), and cytokines TNFα and IL-6 in tissue extracts of carotid plaques in patients after endarterectomy were estimated by Western immunoblotting, after SDS-PAGE analysis and evaluated based on the ultrasonographic plaque morphology. …”
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3720
Optimized sequential model for superior classification of plant disease
Published 2025-01-01“…Our research, conducted on mango and groundnut leaves collected during field visits in western Maharashtra and supplemented by online datasets, demonstrates a CNN model that achieves an impressive 96% accuracy as compared to machine learning techniques that follow tedious feature extraction. …”
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