A method for siRNA-mediated knockdown of target genes in RA-induced neurogenesis using P19 cells by Hossein Khodadadi, Hiroaki Taniguchi

“…Additionally, the efficiency of the siRNA-mediated knockdown was confirmed by western blot analysis, which demonstrated significant gene silencing at protein levels. …”
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Yiqi Fumai Lyophilized injection protects cardiac contractility in a rat model of heart failure with reduced ejection fraction by modulating SERCA2 by Yongwei Nie, Lu Chen, Yanxin Zhang, Yuqing Yao, Zizhuo Xue, Na Guo, Meixiu Wan, Zhi Li, Chunhua Liu, Dekun Li, Aichun Ju

“…Hematoxylin and Eosin (H&E) staining, Masson's trichrome staining, western blotting, and immunohistochemical analysis were performed to evaluate myocardial damage and investigate the underlying molecular mechanisms. …”
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Exosomes derived from liver failure patients’ plasma stimulated mesenchymal stem cells alleviate acute liver failure by Zhuoran Wang, Jun Ling, Shaoli You, Bing Zhu

“…The levels of signaling pathway proteins were determined by western blotting. Results Stimulation with plasma from patients with liver failure significantly altered the morphology of MSCs and reduced their proliferative activity. …”
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Carnitine palmitoyltransferase 1C promotes EMT-associated cisplatin resistance in non-small cell lung cancer cells by Renjie Chen, Jiahui Wang, Shuoyu Huang, Xuefeng Hu, Xinran He, Tiange Zhang, Yunhan Hu, Huijun Wei, Sihui Nian, Yushu Huang, Zhihao Wu

“…Methods: The cell viability assay and apoptosis were used to analyze chemoresistance. Western blot analysis and wound healing testing were used to evaluate the epithelial-to-mesenchymal transition (EMT). …”
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Rice suitability mapping using the analytic hierarchy process approach in a river catchment by N. Robertson, B. Oinam

“…The major patches of such zones are found in the north-western portion of the valley region in the catchment.CONCLUSION: This study clearly indicates, the potential zones lying in the foothills in the north-western which are still not under the agriculture cover have the potential to be cultivated as per the model result. …”
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Mechanism of dexmedetomidine protection against cisplatin induced acute kidney injury in rats by Zeng-lu Zheng, Jun-wei Ma, Yi Luo, Gui-jin Liang, Shi-jie Lei, Ke-jin Yan, Hai-bing Meng, Xiu-juan Liu

“…ELISA analyses demonstrated that Dex treatment reduced the upregulated levels of proinflammatory cytokines in the kidney tissue of AKI rats induced by CP, thereby alleviating kidney tissue injury. Western blotting indicated that Dex alleviated CP-induced AKI by inhibiting pyroptosis mediated by NLRP-3 and caspase-1.Conclusion Dex protected rats from CP-induced AKI, and the mechanism may be related to NLRP-3/Caspase-1-mediated pyroptosis.…”
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Depletion and Reversal of Hepatocellular Carcinoma Inducing CTL through ER Stress-Dependent PERK-CHOP Signaling Pathway by Mengnan Guo, Wei Wang, Wen Bai, Zekun Bai, Weixi Chen, Yali Su, Jinghua Wu

“…The expression of GRP78, PD-1, and TIM-3 was detected by western blotting in CTLL-2 cocultured with Hepa1-6 cells. …”
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LCZ696 Therapy Reduces Ventricular Tachyarrhythmia Inducibility in a Myocardial Infarction-Induced Heart Failure Rat Model by Po-Cheng Chang, Shien-Fong Lin, Yen Chu, Hung-Ta Wo, Hui-Ling Lee, Yu-Chang Huang, Ming-Shien Wen, Chung-Chuan Chou

“…In vivo electrophysiological exams were performed under general anesthesia. Western blot analyses were conducted to quantify ion channel proteins. …”
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Lebanese Cannabis sativa L. extract protects from cisplatin-induced nephrotoxicity in mice by inhibiting podocytes apoptosis by Alia Khalil, Sahar Al Toufaily, Wassim Shebaby, Marissa El Hage, Dima Mroue, Wissam Faour, Mohamad Mroueh

“…Results Cell viability assay and western blot analysis revealed that COE prevented apoptosis induced by cisplatin in cultured immortalized rat podocytes. …”
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Ethanol Extracts of Solanum lyratum Thunb Regulate Ovarian Cancer Cell Proliferation, Apoptosis, and Epithelial-to-Mesenchymal Transition (EMT) via the ROS-Mediated p53 Pathway by Chen Zhang, Zheming Li, Jie Wang, Xuelu Jiang, Mengting Xia, Jianfen Wang, Shenyi Lu, Shouye Li, Hanmei Wang

“…Moreover, DCFH-DA and western blot assays demonstrated that SLT enhanced ROS accumulation and subsequently activated the p53 signaling pathway. …”
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Application of different edge detection methods for faults identification in Anza Basin, Kenya by Gege Li, Qing Chen, Bingqiang Yuan, Xianfeng Tan, Hao Chen, Xiaoyu Huang, Lang Pan

“…Six tectonic zones were recognized in combination with the gravity anomalies and seismic data in the study area, which include eastern depression, southern uplift, central bulge, western depression, northern slope and western uplift.…”
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Ferrostatin-1 inhibits osteoclast differentiation and prevents osteoporosis by suppressing lipid peroxidation by Wenbo Xu, Shiyan Lv, Xiaoyan Wang, Chengchao Song, Chunyang Xi, Jinglong Yan

“…The levels of osteoclastogenesis and lipid peroxides were measured by qRT-PCR, Western Blot and immunofluorescence. Activation of the p38/JNK/MAPK pathway was detected by Western Blot. …”
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Identification of retinol dehydrogenase 10 as a shared biomarker for metabolic dysfunction-associated steatotic liver disease and type 2 diabetes mellitus by Fangyu Li, Rui Li, Hongjun Deng

“…Immunofluorescence and Western blot showed that RDH10 expression was reduced in the liver and pancreatic islets of MASLD and T2DM model mice. …”
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Inequalities in total fertility rate in Sierra Leone, 2008–2019 by Augustus Osborne, Umaru Sesay, Mohamed S. Bah, Camilla Bangura, Bright Opoku Ahinkorah

“…Provincial inequality among women who resided in Western area compared with those in Eastern province decreased slightly from 2.3 births per woman in 2008 to 2.2 births per woman in 2019. …”
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High-performance CT features supporting accurate pre-operative tumor staging in colon cancer by Jianhua Yuan, Cangzheng Jin, Jianrong Si, Baobao Liu, Xiaohan Si, Jianzhi Chen

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Synthesis, Characterization, and DNA-Binding Kinetics of New Pd(II) and Pt(II) Thiosemicarbazone Complexes: Spectral, Structural, and Anticancer Evaluation by Simon N. Mbugua, Lydia W. Njenga, Ruth A. Odhiambo, Shem O. Wandiga, Mervin Meyer, Nicole Sibuyi, Roger A. Lalancette, Martin O. Onani

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Cytotoxicity Potential of Endophytic Fungi Extracts from Terminalia catappa against Human Cervical Cancer Cells by Michèle Stella Majoumouo, Marius Belmondo Tincho, Rufin Marie Kouipou Toghueo, Thureyah Morris, Donavon Charles Hiss, Fabrice Fekam Boyom, Chitra Mandal

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Upregulated SAE1 Drives Tumorigenesis and Is Associated with Poor Clinical Outcomes in Breast Cancer by Hong Liu, Jing Wang, Yunhai Li, Feng Luo, Lei Xing

“…Cell cycle progression was analyzed by flow cytometry, and the expression of cell cycle-related proteins (E2F1, cyclin D3, and cyclin-dependent kinase 2) was determined by western blots in SAE1 small interfering RNA (siRNA) transfected cells. …”
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Early Endoscopic Outcomes After Risankizumab Are Associated With Fewer Hospitalizations and Surgeries in Crohn’s Disease by Brian G. Feagan, Jean-Frederic Colombel, Remo Panaccione, Stefan Schreiber, Marc Ferrante, Koji Kamikozuru, Christopher Ma, Wan-Ju Lee, Jenny Griffith, Namita Joshi, Kristina Kligys, Jasmina Kalabic, Si Xuan, Marla Dubinsky

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Synthesizing Local Capacities, Multi-Source Remote Sensing and Meta-Learning to Optimize Forest Carbon Assessment in Data-Poor Regions by Kamaldeen Mohammed, Daniel Kpienbaareh, Jinfei Wang, David Goldblum, Isaac Luginaah, Esther Lupafya, Laifolo Dakishoni

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