Koumine Attenuates Neuroglia Activation and Inflammatory Response to Neuropathic Pain by Gui-Lin Jin, Sai-Di He, Shao-Mei Lin, Li-Mian Hong, Wan-Qing Chen, Ying Xu, Jian Yang, Su-Ping Li, Chang-Xi Yu

“…Immunofluorescence staining and Western blot analysis were used to assess the modulator effect of koumine on microglia and astrocyte activation after CCI surgery. …”
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Review of climate-resilient agriculture for ensuring food security: Sustainability opportunities and challenges of India by Satiprasad Sahoo, Chiranjit Singha, Ajit Govind, Armin Moghimi

“…Furthermore, the analysis clearly showed that the Western Dry Region, Western Himalayan, and Gangetic Plain agro-ecological zone (AEZ) experienced the sharpest declines in equivalent water thickness (EWT) compared to other AEZ regions in India. …”
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Downregulation of TNF-α/TNF-R1 Signals by AT-Lipoxin A4 May Be a Significant Mechanism of Attenuation in SAP-Associated Lung Injury by Suhui Yu, Jianming Xie, Yukai Xiang, Shengjie Dai, Dinglai Yu, Hongwei Sun, Bicheng Chen, Mengtao Zhou

“…However, the anti-inflammatory effects of AT-Lipoxin A4 on SAP-associated lung injury are not thoroughly known. We used western blot, polymerase chain reaction (PCR), and immunofluorescence to investigate the downregulation of TNF-α signals in cellular and animal models of SAP-associated lung injury following AT-Lipoxin A4 intervention. …”
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Protective Effect of Thalidomide on 2,4,6-Trinitrobenzenesulfonic Acid-Induced Experimental Colitis in Rats via the Inhibition of T Helper 17 Cells by Ying Xie, Dandan Li, Xiaoshuang Luan, Shuang Jin, Bo Yan, Feng Tian

“…Analytical testing (immunohistochemistry, western blot, and qRT-PCR) shows that IL-6, IL-17, IL-23, and RORγt protein and mRNA expression levels were significantly reduced by thalidomide (p<0.05 for all) and that these levels were significantly lower in the high-dose thalidomide group than in the low-dose thalidomide group (p<0.05 for all). …”
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Osimertinib exacerbates immune checkpoint inhibitor-related severe adverse events by activating the IL-6/JAK/STAT3 pathway in macrophages by Yuan Li, Yanping Chen, Yuan Meng, Meng Shen, Fan Yang, Xiubao Ren

“…Total RNA extracted from mouse liver macrophages was analyzed by RNA-seq. Simple Western blot analysis was used to detect the IL-6/JAK/STAT3 pathway activation state. …”
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20-HETE mediates Ang II-induced cardiac hypertrophy via ROS and Ca2+ signaling in H9c2 cells by Jingyi Han, Jiaojiao Li, Lianlian Liu, Kaiyuan Li, Chun Zhang, Yong Han

“…The ERK/Akt and CaN/NFAT3 signaling pathways were analyzed through Western blot. Ang II was found to promote CYP4A expression and 20-HETE production in H9c2 cells via an AT1 receptor-dependent mechanism. …”
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PD-L1 monoclonal antibody alleviated MI injury of left ventricular function via modulating CD47/SHP2/SIRPα/SYK/FcγR signalings in tumor associated macrophages by Deyou Kong, Yongzhen Chen, Yajuan Yin, Zhikun Liu, Fang Yang, Xiaohong Li, Dongxing Shen, Jun Zhang

“…Protein expression levels in each group were analyzed by Western blotting, and the macrophages were transplanted into experimental mice for observation. …”
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Expression of the Gene Encoding the Brucella abortus BAB1-0278 Antigen in Probiotic Lactococcus lactis Bacteria by donya kazemi, abbas doosti, mostafa Shakhsi niaee

“…Conclusion: The results of PCR, restricted enzyme digestion, and sequencing by GENEray revealed that BAB1-0278 was cloned correctly in pNZ8148-Usp45. SDS-PAGE and Western blot were used to analyze BAB1-0278 protein expression. …”
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High hemolytic activity in Staphylococcus aureus t1081/ST45 due to increased hla protein production and potential RNAIII-independent regulation by Yu-Tzu Lin, Ngoc-Niem Bui, Yu-Syuan Cheng, Cheng-Wen Lin, Chun-Li Lee, Tai-Fen Lee, Po-Ren Hsueh

“…The expression of the transcriptional regulators mgrA and saeR was also similar between the two groups. Western blotting revealed increased Hla protein in the t1081 isolates. …”
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β-Hydroxybutyrate Protects Against Cisplatin-Induced Renal Damage via Regulating Ferroptosis by Ruixue Tian, Shuqin Tang, Jingyu Zhao, Yajie Hao, Limei Zhao, Xiutao Han, Xingru Wang, Lijun Zhang, Rongshan Li, Xiaoshuang Zhou

“…We analyzed blood urea nitrogen (BUN) and creatinine (Cr) levels in serum, and used western blotting and immunohistochemical staining to assess ferroptosis and the calcium/calmodulin-dependent kinase kinase 2 (Camkk2)/AMPK pathway. …”
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Unpacking the ‘explorer’ narrative and its impacts on African palaeoanthropology by Rebecca R. Ackermann, Keneiloe Molopyane, Sheela Athreya

“…This is especially notable in fieldwork practices which, to this day, embody the explorer myth’s deeply problematic colonial ideals of Western, masculine moral and cultural superiority. …”
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Atrial flutter/fibrillation in adult congenital heart disease: Insights from a large Asian cohort by Shuenn-Nan Chiu, Wei-Chieh Tseng, Chun-Wei Lu, Ming-Tai Lin, Chun-An Chen, Jou-Kou Wang, Mei-Hwan Wu

“…Background: The incidence of atrial fibrillation/atrial flutter (AF/AFL) in general population is lower in Asia compared to Western countries. It is unclear whether a similar trend exists among adults with congenital heart disease (ACHD). …”
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Accelerated Warming and Salinification of the Mediterranean Sea: Implications for Dense Water Formation by Nikolaos Skliris, Robert Marsh, Matthew Breedon, Simon A. Josey

“…Results show much larger trends over 2004–2023 when a broadscale decrease in sensible heat flux (<i>SHF</i>) is obtained over the western basin as the air temperature is increasing much faster than <i>SST</i>. …”
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Plasminogen Activator Inhibitor-1 Regulates LPS Induced Inflammation in Rat Macrophages through Autophagy Activation by Zhong-Hui Wang, Wei-Ying Ren, Lei Zhu, Li-Juan Hu

“…ELISA was used to assess the amounts of TNF-α, IL-1β, and PAI-1 in cell culture supernatants; TLR4, MyD88, PAI-1, LC3, Beclin1, and mTOR protein and mRNA levels were determined by western blot and quantitative RT-PCR, respectively; western blot was used to determine NF-κB protein levels. …”
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Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions by Arjun Sinkemani, Feng Wang, Zhiyang Xie, Lu Chen, Cong Zhang, Xiaotao Wu

“…Expression was investigated using quantitative polymerase chain reaction (qPCR) and western blotting under normoxic and hypoxic conditions. qPCR and western blotting demonstrated significant upregulation of NP marker expression in MSCs cultured under hypoxic conditions and treated with the 50% or 100% NPC conditioned medium, compared with those cultured under normoxic conditions. …”
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Endoplasmic Reticulum Stress Regulates Scleral Remodeling in a Guinea Pig Model of Form-Deprivation Myopia by Chengcheng Zhu, Qingzhong Chen, Ying Yuan, Min Li, Bilian Ke

“…The protein levels of ER stress chaperones, including GRP78, CHOP, and calreticulin (CRT), were analyzed by western blotting at 24 hours, 1 week, and 4 weeks of FD. …”
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Mechanistic insights into epithelial-mesenchymal transition mediated cisplatin resistance in ovarian cancer by Taha Umair Wani, Hyun-Yi Kim, Geum-Hwa Lee, Young Je Lim, Han-Jung Chae, Ji-Ye Kim, Hyonok Yoon

“…We validated our results by western blotting and found that ITGA1 was highly expressed in resistant cells. …”
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Geochemistry and Genesis of Oil and Gas Seeps in the Junggar Basin, NW China: Implications for Hybrid Petroleum Systems by Jingkun Zhang, Jian Cao, Yan Wang, Jun Li, Guang Hu, Ni Zhou, Tianming Shi

“…Type 5 oil seeps mainly have Paleogene source rocks with a minor contribution from Jurassic rocks and occur in the Wusu and Dushanzi areas in the western segment of the southern basin with the single-type gas seeps. …”
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Decreased Spp1 Expression in Acute Myocardial Infarction after Ischemia and Reperfusion Injury by Ling Li, Jungang Huang, Zongkai Zhao, Zhuzhi Wen, Kang Li, Tianjiao Ma, Lisui Zhang, Junmeng Zheng, Shi Liang

“…In the end, we used quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blotting to measure and compare the expression of Spp1 in patients who accepted percutaneous coronary intervention. …”
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Heavy Metal Spatial Variation Mechanism and Ecological Health Risk Assessment in Volcanic Island Soils: A Case Study of Weizhou Island, China by Ran Bi, Wei Fu, Xuanni Fu

“…Heavy metal concentrations in Weizhou Island soils are notably elevated at both the western wharf and the island’s tail, both characterized by volcaniclastic rock lithology, with human activities further increasing concentrations at the western wharf compared to the island’s tail. …”
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