Structure- and machine learning-guided engineering demonstrate that a non-canonical disulfide in an anti-PD-1 rabbit antibody does not impede antibody developability by Wei-Ching Liang, Hongkang Xi, Dawei Sun, Luigi D’Ascenzo, Jonathan Zarzar, Nicole Stephens, Ryan Cook, Yinyin Li, Zhengmao Ye, Marissa Matsumoto, Jian Payandeh, Matthieu Masureel, Yan Wu

“…Initial removal of the non-canonical disulfide resulted in a loss of PD-1 affinity and cross-reactivity, which led us to explore protein engineering approaches to recover these. …”
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Bayesian joint-regression analysis of unbalanced series of on-farm trials by Turbet Delof, Michel, Rivière, Pierre, Dawson, Julie C, Gauffreteau, Arnaud, Goldringer, Isabelle, van Frank, Gaëlle, David, Olivier

“…This statistical framework allowed us to estimate two indicators of genotype stability (one static and one dynamic) despite the high disequilibrium of the data. …”
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Construire des ressources relationnelles pour franchir des frontières multiples : Trajectoires de jeunes Tunisiens by Hasnia-Sonia Missaoui

“…These trajectories show us first of all the disenchantment of an age group with blurred contours that is linked to the situations of socio-economic injustice that they experience on a daily basis. …”
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Pairing regression and configurational analysis in health services research: modelling outcomes in an observational cohort using a split-sample design by Jason J Sico, Laura J Myers, Dawn M Bravata, Ying Zhang, Anthony J Perkins, Edward J Miech, Joanne Daggy

“…Background Configurational methods are increasingly being used in health services research.Objectives To use configurational analysis and logistic regression within a single data set to compare results from the two methods.Design Secondary analysis of an observational cohort; a split-sample design involved randomly dividing patients into training and validation samples.Participants and setting Patients who had a transient ischaemic attack (TIA) in US Department of Veterans Affairs hospitals.Measures The patient outcome was the combined endpoint of all-cause mortality or recurrent ischaemic stroke within 1 year post-TIA. …”
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Modeling dose uncertainty in cone-beam computed tomography: Predictive approach for deep learning-based synthetic computed tomography generation by Cédric Hémon, Lucía Cubero, Valentin Boussot, Romane-Alize Martin, Blanche Texier, Joël Castelli, Renaud de Crevoisier, Anaïs Barateau, Caroline Lafond, Jean-Claude Nunes

“…Additionally, to explore potential error scenarios, we generated several ‘plausible’ sCTs representing variations in sCT generation caused by CBCT quality differences. This allowed us to quantify dose uncertainties. Results:: The effectiveness of uncertainty maps was evaluated by correlating them with the absolute error map between sCT and CT, yielding a Pearson correlation coefficient between 0.65 and 0.72. …”
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Association between Glycemic Control and Clinic Attendance in Emerging Adults with Type 1 Diabetes: A Tertiary Center Experience by Eldad Fisher, Liora Lazar, Shlomit Shalitin, Michal Yackobovitch-Gavan, Liat de Vries, Tal Oron, Ariel Tenenbaum, Moshe Phillip, Yael Lebenthal

“…Our institutional policy offering surveillance till age 30 enabled us to evaluate clinic attendance without the confounding effect of transition. …”
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Associations between oxidation balance score and abdominal aortic calcification, and the mediating role of glycohemoglobin: a nationally representative cross-sectional study from N... by Heqian Liu, Yifei Wu, Zhenyu Liu, Hongzhi Ren, Ya Wu, Ya Wu, Yong Liu, Yong Liu, Yong Liu

“…Our aim was to evaluate whether subjective interventions could influence AAC scores by modifying the antioxidant/pro-oxidant status of individuals and to investigate the role of glycohemoglobin in this relationship.MethodsThe study analyzed data from 1,600 U.S. adults. The study used oxidation balance score (OBS) as an exposure variable derived from 16 dietary and 4 lifestyle factors, as well as glycohemoglobin obtained from blood tests. …”
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Human Factors Studies to Assess the Usability of the Faricimab Prefilled Syringe by Barakat MR, Kwong Jr HM, Marcon G, O'Leary OE, Paris LP, Schneider P, Tang Y, Graff JM

“…Injections were performed into single eyes of 35 patients with nAMD or DME in three US clinics and patients were followed for 7 days for safety reporting (ClinicalTrials.gov identifier: NCT05569148).Results: In the simulated-use study, most retina specialists and ophthalmic assistants completed all tasks deemed essential for PFS preparation and administration correctly and without error. …”
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Trends in depressive symptoms among high school students with and without health-risk behaviors in the United States: A population-based studyResearch in context by Tanner J. Bommersbach, Mark Olfson, Taeho Greg Rhee

“…Methods: Data were analyzed from the 2007 to 2021 Youth Risk Behavior Survey (YRBS), a biennial cross-sectional survey of US high school students (n = 119,654). Depressive symptoms were defined as past-year feelings of sadness/hopelessness almost every day for ≥2 weeks impacting usual activities. 24 health-risk behaviors across five categories (violence, substance use, sexual, physical/sedentary, and weight-related) were considered. …”
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Valle de inmigrantes enjaulados: castigo, protesta y la aparición del Centro de Detención Puerto Isabel by Jennifer Cullison

“…Con el mayor apoyo del congreso a los controles a la inmigración, para la década de 1970, no solo el INS, sino también el Servicio de Alguaciles estadounidenses (US Marshals Service), y la Oficina de Prisiones de Estados Unidos tuvo mayores números de personas encerradas invocando el código legal de inmigración. …”
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A single-cell and spatial wheat root atlas with cross-species annotations delineates conserved tissue-specific marker genes and regulators by Yuji Ke, Vincent Pujol, Jasper Staut, Lotte Pollaris, Ruth Seurinck, Thomas Eekhout, Carolin Grones, Maite Saura-Sanchez, Michiel Van Bel, Marnik Vuylsteke, Andrea Ariani, Christophe Liseron-Monfils, Klaas Vandepoele, Yvan Saeys, Bert De Rybel

“…The predictions from our orthology-based annotation approach were next validated using untargeted spatial transcriptomics. These results allowed us to predict evolutionarily conserved tissue-specific markers and generate cell type-specific gene regulatory networks for root tissues of wheat and the other species used in our analysis. …”
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Buffalo whey proteins as innovative raw material for nanoparticles building by Pablo A. Solis Cizek, Gustavo A. Crudeli, Darío Spelzini, Franco E. Vasile

“…Obtained results allow us to consider that BWPC can undergo associative interactions with GA, resulting in soluble nano complexes with good colloidal valuable stability for the nutraceutical industry. …”
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Insights into the relations between cell wall integrity and in vitro digestion properties of granular starches in pulse cotyledon cells after dry heat treatment by Ping Li, Bin Zhang, Rui Liu, Li Ding, Xiong Fu, Haiteng Li, Qiang Huang, Xiaowei He

“…Natural foods, such as whole pulses, are recommended in the dietary guidelines of the US and China. The plant cell wall structure in whole pulses has important implications for the nutritional functionalities of starch. …”
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Language and Culture in Health Literacy for People Living with HIV: Perspectives of Health Care Providers and Professional Care Team Members by Keitshokile Dintle Mogobe, Sheila Shaibu, Ellah Matshediso, Motshedisi Sabone, Esther Ntsayagae, Patrice K. Nicholas, Carmen J. Portillo, Inge B. Corless, Carol Dawson Rose, Mallory O. Johnson, Allison Webel, Yvette Cuca, Marta Rivero-Méndez, Solymar S. Solís Báez, Kathleen Nokes, Darcel Reyes, Jeanne Kemppainen, Paula Reid, Lucille Sanzero Eller, Teri Lindgren, William L. Holzemer, Dean Wantland

“…Data were collected from sites in Botswana, the US, and Puerto Rico. In the parent study, data were collected through focus group discussions with 135 people living with HIV, 32 HIV health care providers (HCPs), and 39 HIV professional care team members (PCTMs). …”
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Real-world enrollment for a prospective clinico-genomic database using a pragmatic technology-enabled platform by Alexia Exarchos, Ariel B. Bourla, Maneet Kaur, Katja Schulze, Sophia Maund, Yi Cao, Yihua Zhao, Elizabeth H. Williams, Sarah C. Gaffey, Richard Zuniga, Shaily Lakhanpal, Vladan Antic, Michelle Doral, Johanna Sy, Neal J. Meropol, Anne C. Chiang

“…Methods: Adults with stage IV or recurrent metastatic non-small cell lung cancer or extensive-stage small-cell lung cancer were enrolled at 23 US sites upon initiating a standard line of therapy. …”
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The Association Between the CALLY Index and All-Cause Mortality in Patients with COPD: Results from the Cohort Study of NHANES 2007–2010 by Ding Y, Liu Y, Yu J, Cai C, Fu L, Zhu J, Yang S, Jiang Y, Wang J

“…The areas under the curve (AUC) in predicting 5- and 10-year all-cause mortality were 0.693 and 0.656.Conclusion: The CALLY index has a strong relationship with all-cause mortality in patients with COPD in the US and could serve as a prognostic biomarker for these patients.Keywords: chronic obstructive pulmonary disease, the C-reactive protein–albumin–lymphocyte index, national health and nutrition examination survey, biomarker…”
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Gene networks and metabolomic screening analysis revealed specific pathways of amino acid and acylcarnitine profile alterations in blood plasma of patients with Parkinson’s disease... by A. A. Makarova, P. M. Melnikova, A. D. Rogachev, P. S. Demenkov, T. V. Ivanisenko, E. V. Predtechenskaya, S. Y. Karmanov, V. V. Koval, A. G. Pokrovsky, I. N. Lavrik, N. A. Kolchanov, V. A. Ivanisenko

“…Comparative analysis of molecular genetic pathways in gene networks allowed us to identify both specific and non­specific molecular mechanisms associated with changes in the metabolomic profile in PD and VP. …”
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Pharmacognostic analysis and antimalarial evaluation of quercetin in Ilex umbellulata bark using HPTLC, in vitro screening, molecular docking, and network pharmacology by James H. Zothantluanga, Dipak Chetia, Yasangam Umbon, T. C. Lalhriatpuii, Dhritiman Roy, Nidahun Lamare, Salem Lalvenhimi

“…JazQSAR web tool previously developed by us predicts the IC50 of quercetin against Plasmodium falciparum as 3.88 ± 0.35 µM, which was not far from the practically observed value for quercetin. …”
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Achieving healthy aging through gut microbiota-directed dietary intervention: Focusing on microbial biomarkers and host mechanisms by Yue Xiao, Yingxuan Feng, Jianxin Zhao, Wei Chen, Wenwei Lu

“…Comprehensive understanding on changes of gut microbiota during aging, in particular gut microbiota characteristics of centenarians, can provide us possibility to achieving healthy aging or intervene pathological aging through gut microbiota-directed strategies. …”
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Personality's Time Perspective as a Resource for Overcoming the Identity Crisis at Different Stages of Life Realization by E.M. Meshcheryakova, A.V. Seryy, M.S. Yanitskiy

“…</strong>A set of diagnostic techniques aimed at studying the features of a personality's time perspective and the specifics of the manifestations of experiencing identity crises allowed us to collect data on samples formed in accordance with age periods. …”
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