Mechanism of Inhibition of Inflammation in Osteoarthritis by Tougu Xiaotong Capsule via Regulating Ras-Raf-MEK1/2-ERK1/2 Signaling Pathway by Munan LIN, Xiang SHAO, Limei XU, Xin ZHANG, Zaishi ZHU, Yidan HAN, Xinwei DUAN, Jianmei CHEN, song GAO, Xihai LI

“…Isolated chondrocytes of passage two were divided into blank group, model group(treatment with 10 ng/mL of LPS for 8 h), inhibitor group(treatment with 10 ng/mL of U0126 for 30 min followed by 10 ng/mL of LPS for additional 8 h)and TXC group(treatment with 10 ng/mL of LPS plus 300 μg/mL of TXC for 8 h).Western blot was carried out to detect the expression of Ras, Raf, p-ERK1/2, MEK1/2, MMP-3 and MMP-13.qPCR was used to detect the mRNAs expression of MEK1/2, ERK1/2, MMP-3 and MMP-13, as well as the expression of miR-27b, miR-34a and miR-146a.Results:1)The cytoplasm of isolated cells(P2)from the cartilage of knee joint of 4-week-old SD male rats was stained brown by typeⅡcollagen immunohistochemical staining as compared with the negative control cells, which indicated that the extracted cells were chondrocytes.2)The expression of Ras, Raf, MEK1/2, MMP-3, MMP-13 in the model group was significantly higher than that in the blank group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05), but the expression of p-ERK1/2 had no significant difference(<italic>P</italic>&gt;0.05);the expression of Ras, Raf, p-ERK1/2, MEK1/2, MMP-3, MMP-13 in both the inhibitor group and the TXC group was lower than that in the model group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05), but the expression of MEK1/2 in the TXC group had no significant difference compared to that in the model group(<italic>P</italic>&gt;0.05).3)The mRNA expression of MEK1/2, ERK1/2, MMP-3 and MMP-13 in the model group was significantly higher than that in the blank group, respectively(<italic>P</italic>&lt;0.01);their expression in both the inhibitor group and the TXC group was lower than that in the model group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05).4)The expression of miR-27b in the model group was significantly lower than that in the blank group(<italic>P</italic>&lt;0.01), but its expre-ssion in both the inhibitor group and the TXC group was higher than that in the model group(<italic>P</italic>&lt;0.01);the expression of both miR-34a and miR-146a in the model group was significantly higher than that in the blank group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05);however, their expression in both the inhibitor group and the TXC group was lower than that in the model group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05).Conclusion:Tougu Xiaotong capsule regulates the expression of key factors in Ras-Raf-MEK1/2-ERK1/2 signaling pathway and miR-27b, miR-34a and miR-146a to regulate the inflammatory of chondrocytes, thereby inhibiting osteoarthritis inflammation and protecting articular cartilage.…”
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Design of Command and Dispatch System for Automatic Reading of Meter Images in Substations by UAV Inspection Photos by Guanghong Deng, Liangpei Lin, Baihao Lin, Wenlong Jing, Xiaodan Zhao, Yong Li, Rui Yang, Shidun Xie, Pengfeng Wei

“…The mean intersection over union (mIoU) of the image segmentation model reached 82.00%, 74.73%, 73.50%, 82.26% and 73.20%, respectively, and the single segmentation speed reached 33.7[Formula: see text]ms. …”
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Value of triglyceride glucose-body mass index in predicting nonalcoholic fatty liver disease in individuals with type 2 diabetes mellitus by Xiaoyi Qian, Wenwen Wu, Boyang Chen, Simin Zhang, Chunmei Xiao, Long Chen, Jun Chen, Jun Chen, Lingli Ke, Meian He, Xiulou Li, Xiulou Li, Xiulou Li

“…Patients were categorized into two groups: T2DM alone (n=250) and T2DM with NAFLD (n=352). The Mann-Whitney U test was used for comparing non-normally distributed continuous data between groups, while the Chi-square test was used for categorical data. …”
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Development of an in vitro model to study muscle maladaptation to overtraining by Giuseppe Sirago, Clément Lanfranchi, Justin Carrard, Vincent Gremeaux, Nadège Zanou, Nicolas Place

“…., Donnelly, C., De Marchi, U., Ferrini, M., Vidal, J., Sittenfeld, L., Feige, J. …”
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On Channel Transforms to Enhance Reciprocity and Quantization in Physical-Layer Secret Key Generation by Ghalib Hussain, Syed Junaid Nawaz, Shurjeel Wyne, Mohammad N. Patwary

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Matrine Inhibits Breast Cancer Cell Proliferation and Epithelial-Mesenchymal Transition Through Regulating the LINC01116/miR-9-5p/ITGB1 Axis by Lili Ren, Ziru Fang, Jiaojiao Xu, Xiaoxiao Wu, Yongjun Zhang, Hu Cai, Zhicun Han

“…Functional assays such as CCK-8, EdU, Transwell, Western blot, and flow cytometry assays were performed on the BC cells, and the impacts of LINC01116, miR-9-5p, and ITGB1 expression levels on MAT’s mechanism of action were assessed. …”
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System-Technology Co-Optimization for Dense Edge Architectures Using 3-D Integration and Nonvolatile Memory by Leandro M. Giacomini Rocha, Mohamed Naeim, Guilherme Paim, Moritz Brunion, Priya Venugopal, Dragomir Milojevic, James Myers, Mustafa Badaroglu, Marian Verhelst, Julien Ryckaert, Dwaipayan Biswas

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The interaction of tPA with NMDAR1 drives neuroinflammation and neurodegeneration in α-synuclein-mediated neurotoxicity by Daniel Torrente, Enming J. Su, Alí Francisco Citalán-Madrid, Gerald P. Schielke, Daniel Magaoay, Mark Warnock, Tamara Stevenson, Kris Mann, Flavie Lesept, Nathalie Delétage, Manuel Blanc, Erin H. Norris, Denis Vivien, Daniel A. Lawrence

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Clinical novel exploration of intractable fever in stroke rehabilitation: a single-center cross-sectional retrospective study by Huang Jingjie, Wu Bangqi, Qin Peng, Zhang Ziyi, Cheng Yupei, Wang Chaoran, Chen Yuyan, Bai Jing

“…Medical records and clinical characteristics were collected, and the data from the two groups of febrile patients were analyzed using t-tests, Mann-Whitney U tests, and chi-square tests. Logistic regression analysis was performed to identify risk factors associated with intractable fever, while receiver operating characteristic (ROC) curves were used to assess the predictive performance of individual and combined risk factors. …”
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Age dependent deficits in speech recognition in quiet and noise are reflected in MGB activity and cochlear onset coding by Konrad Dapper, Stephan M. Wolpert, Jakob Schirmer, Stefan Fink, Etienne Gaudrain, Deniz Başkent, Wibke Singer, Sarah Verhulst, Christoph Braun, Ernst Dalhoff, Lukas Rüttiger, Matthias H.J. Munk, Marlies Knipper

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Deep cascaded registration and weakly-supervised segmentation of fetal brain MRI by Valentin Comte, Mireia Alenya, Andrea Urru, Judith Recober, Ayako Nakaki, Francesca Crovetto, Oscar Camara, Eduard Gratacós, Elisenda Eixarch, Fatima Crispi, Gemma Piella, Mario Ceresa, Miguel A. González Ballester

“…Furthermore, we integrate our registration method into a multi-atlas segmentation pipeline and showcase its competitive performance compared to nnU-Net, achieved using only a small subset of annotated images as atlases. …”
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Differentiation between glioblastoma and solitary brain metastases using perfusion and amide proton transfer weighted MRI by Malte Knutsson, Tim Salomonsson, Faris Durmo, Emelie Ryd Johansson, Anina Seidemo, Jimmy Lätt, Anna Rydelius, Sara Kinhult, Elisabet Englund, Johan Bengzon, Peter C. M. van Zijl, Peter C. M. van Zijl, Linda Knutsson, Linda Knutsson, Linda Knutsson, Pia C. Sundgren, Pia C. Sundgren, Pia C. Sundgren

“…Statistical analyses were performed using Mann–Whitney U test.ResultsWhen comparing GBM to MET, nAPTmax, nCBFmax, ncCBVmax and ncCBVmean were significantly increased (p &lt; 0.05) in ET with AUC being 0.81, 0.83, 0.85, and 0.83, respectively. …”
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Exploring the ability of plasma pTau217, pTau181 and beta-amyloid in mirroring cerebrospinal fluid biomarker profile of Mild Cognitive Impairment by the fully automated Lumipulse®... by Marcella Catania, Claudia Battipaglia, Alberto Perego, Erika Salvi, Emanuela Maderna, Federico Angelo Cazzaniga, Paolo M. Rossini, Camillo Marra, Nicola Vanacore, Alberto Redolfi, Daniela Perani, Patrizia Spadin, Maria Cotelli, Stefano Cappa, Naike Caraglia, Pietro Tiraboschi, Fabrizio Tagliavini, Giuseppe Di Fede

“…Abstract Background The approval of new disease-modifying therapies by the U.S. Food and Drug Administration and the European Medicine Agency makes it necessary to optimize non-invasive and cost-effective tools for the identification of subjects at-risk of developing Alzheimer’s Disease (AD). …”
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Performance of the AmpliSeq NGS panel in thyroid nodules with indeterminate cytology by Wiame Potonnier, Erell Guillerm, Claude Bigorgne, Cécile Ghander, Malanie Roy, Florence Coulet, François Ansart, Fabrice Menegaux, Laurence Leenhardt, Isabelle Brocheriou, Gabrielle Deniziaut, Camille Buffet

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Impact of co-infections and immune responses on clinical severity of human adenovirus 3 and 7 infections in hospitalized children with lower respiratory tract infections: a compara... by Xiaolin Ma, Yuting Wu, Ri De, Hailan Yao, Feng He, Yi Wang, Wei Wang, Chao Yan, Qinwei Song, Chunjie Guo, Li Wen, Linqing Zhao, Ling Cao, Chunmei Zhu

“…HAdV-7-infected patients exhibited more prolonged fever (100% vs 89.4%, p=0.014), pneumonia (100% vs 89.4%, p=0.014), hypoxia (34.5% vs 12.1%, p=0.003), higher propensity for aspartate aminotransferase exceeding 80U/L (21.1% vs 4.7%, p=0.006), D-Dimer exceeding 1.65mg/L (64.9% vs 12.5%, p&lt;0.001), consolidation (50.0% vs 27.4%, p=0.011), and pleural effusion (32.8% vs 6.5%, p&lt;0.001), co-infections with Mycoplasma pneumoniae (77.1% vs 32.6%, p&lt;0.001), and multiple infections (56.8% vs 41.3%, p=0.007), compared to those with HAdV-3 infections. …”
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Key Performance Indicators in Freestyle Swimming: Comparing Start, Turn, and Swimming Velocity in Top-Elite Female Junior and Adult Swimmers by Chantal Widmer, Julia Hernandez, Michael Romann, Wolfgang Taube, Dennis-Peter Born

“…While the effect of start performance decreased from short to long race distances, turn performances showed a U-shaped, hence largest effect on middle-distance races, i.e. 200 m and 400 m. …”
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Adverse reactions of botulinum toxin type A for treating strabismus in children by E.E. Sidorenko, I.A. Ostanina, K.N.M. Rosel’o, D.V. Migel, E.I. Sidorenko

“…BTA was injected in horizontal extraocular muscles (average dose 3.40 U).</i> </p> <p> <i><b>Results</b>: the average angle of strabismus was 33.28±12.96°, the average angle of eso-tropia was 33.69±12.86°, the average angle of exotropia was 31.50±13.41°. …”
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Kıraat İlminde Râ (ر) Harfi: Son Dönem Osmanlı Kurrâlarından Tedkîk-i Mesâhif-i Şerîfe Meclisi Âzası Bursalı Süleyman Vehbi Efendi’nin Risâle-i Râiyye Adlı Eseri Özelinde Bir İncel... by Osman Bostan

“…Makalenin Risâle-i Râiyye çerçevesinde ele alınmasının sebebi müellifin, Osmanlı’daki kıraat tedrisinde temel kaynak olarak kullanılan en-Neşr, İthâf, Zübdetü’l-irfân, Umdetü’l-hullân, el-Îtilâf, Bedâi‘u’l-burhân ve Mürşidü’t-talebe gibi eserlerin hemen hepsine müracaat edip konuyu sistematik bir şekilde ele alması ve yeri geldikçe mezkûr eserlerdeki hata ve eksikliklere değinmesidir. …”
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How Antiretroviral Drug Concentrations Could Be Affected by Oxidative Stress, Physical Capacities and Genetics: A Focus on Dolutegravir Treated Male PLWH by Jessica Cusato, Anna Mulasso, Micol Ferrara, Alessandra Manca, Guido Accardo, Alice Palermiti, Miriam Antonucci, Gianluca Bianco, Francesco Chiara, Jacopo Mula, Maria Cristina Tettoni, Simone Cuomo, Giulia Trevisan, Stefano Bonora, Giovanni Di Perri, Corrado Lupo, Alberto Rainoldi, Antonio D’Avolio

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Association of handgrip strength asymmetry and weakness with intrinsic capacity impairment among older adults in China by Decheng Li, Yunhe Wang, Shuai Guo, Ziyang Ren, Binbin Su, Lichuan Zhang, Zuliyaer Talifu, Xiaoying Zheng

“…In contrast to other domains, it was the Weakness only group, rather than the Both group, that had the highest risk of vitality impairment. There was a U-shaped relationship between HGS asymmetry and IC impairment. …”
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