Multidirectional sexual dimorphism for the live weight in domestic pigs by S. V. Nikitin, S. P. Knyazev, K. S. Shatokhin, G. M. Goncharenko, V. I. Ermolaуev

“…In particular, the average weight of wild boar females is about 80% of that of the males. …”
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Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production by Duck Gyun Kim, Boncheol Gu, Yujin Cha, Jeonghan Ha, Yongjae Lee, Gahyeon Kim, Byung-Kwan Cho, Min-Kyu Oh

“…The Cas9-BD, a modified Cas9 with the addition of polyaspartate to its N- and C-termini, is developed with decreased off-target binding and cytotoxicity compared with wild-type Cas9. Cas9-BD and similarly modified dCas9-BD have been successfully employed for simultaneous biosynthetic gene cluster (BGC) refactoring, multiple BGC deletions, or multiplexed gene expression modulations in Streptomyces. …”
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Changes in Lysine Methylation Contribute to the Cytotoxicity of Curcumin in Colon Cancer Cells by Roberta Santarelli, Paola Currà, Michele Di Crosta, Roberta Gonnella, Maria Saveria Gilardini Montani, Mara Cirone

“…This correlated with the downregulation of methyltransferases EZH2, MLL1, and G9a, in both wild-type p53 (wtp53) HCT116 cells and mutant p53 (mutp53) SW480 cells, as well as SET7/9 specifically in wtp53 HCT116 cells. …”
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Direct Ethanol Production from Lignocellulosic Sugars and Sugarcane Bagasse by a Recombinant Trichoderma reesei Strain HJ48 by Jun Huang, Dong Chen, Yutuo Wei, Qingyan Wang, Zhenchong Li, Ying Chen, Ribo Huang

“…The initial mutant population was generated by nitrosoguanidine treatment of the spores, and an improved population producing more than fivefold ethanol than wild type was obtained by genome shuffling. The results show that the shuffled strain HJ48 can efficiently convert lignocellulosic sugars to ethanol under aerobic conditions. …”
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Sperm Cryopreservation in Canaries to Protect Endangered Songbird Species: Comparison of Different Cryoprotectants by Arda Onur Özkök, Burcu Esin, Eser Akal

“…ABSTRACT Sperm cryopreservation is a rather complex process that needs to be adapted to wild and domestic bird species to ensure adequate efficiency. …”
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Arachis hypogaea monoacylglycerol lipase AhMAGL3b participates in lipid metabolism by Yihua Zhan, Jing Wang, Xuan Zhao, Zhifu Zheng, Yi Gan

“…A total of 9 homozygous T3 generation transgenic lines were obtained. Compared with wild type (WT), overexpression (OE) of AhMAGL3b had no obvious growth inhibition by investigation of agronomic traits, including growth and photosynthetic parameters. …”
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A NOTCH3 pathogenic variant influences osteogenesis and can be targeted by antisense oligonucleotides in induced pluripotent stem cells. by Ernesto Canalis, Jungeun Yu, Lauren Schilling, Paymaan Jafar-Nejad, Michele Carrer

“…ASOs targeting NOTCH3 decreased both NOTCH3 wild type and NOTCH36692-93insC mutant mRNA by 40% in mesenchymal and 90% in osteogenic cells. …”
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Gut Microbiome and Inflammation: A Study of Diabetic Inflammasome-Knockout Mice by Roma Pahwa, Miriam Balderas, Ishwarlal Jialal, Xinpu Chen, Ruth Ann Luna, Sridevi Devaraj

“…Gut microbiome composition and biomarkers of inflammation (IL-18, serum amyloid A) were assessed in streptozotocin- (STZ-) induced diabetic mice on a NLRP3-knockout (KO) background versus wild-type diabetic mice. Results. SAA and IL-18 levels were significantly elevated in diabetic mice (STZ) compared to control (WT) mice, and there was a significant attenuation of inflammation in diabetic NLRP3-KO mice (NLRP3-KO STZ) compared to control mice (p<0.005). …”
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Liquid Chromatography-Tandem Mass Spectrometry Analysis of Perfluorooctane Sulfonate and Perfluorooctanoic Acid in Fish Fillet Samples by Viviana Paiano, Elena Fattore, Andrea Carrà, Caterina Generoso, Roberto Fanelli, Renzo Bagnati

“…The method was applied to samples of homogenized fillets of wild and farmed fish from the Mediterranean Sea. …”
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Enhanced silk production and pupal weight in Bombyx mori through CRISPR/Cas9-mediated circadian Clock gene disruption. by Daniel J Brady, Alessio Saviane, Matteo Battistolli, Irene Varponi, Federica Barca, Kunihiro Shiomi, Silvia Cappellozza, Federica Sandrelli

“…Importantly, ClkΔ29 mutants reared on a standard sericulture diet exhibited significant increases in key economic traits, with silk production increasing by up to 7%, and pupal weight increasing by up to 25% compared to wild-type controls. This study highlights the potential of circadian clock gene manipulation to significantly enhance sericultural productivity. …”
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The Maize Corngrass1 miRNA-Regulated Developmental Alterations Are Restored by a Bacterial ADP-Glucose Pyrophosphorylase in Transgenic Tobacco by Ayalew Ligaba-Osena, Kay DiMarco, Tom L. Richard, Bertrand Hankoua

“…The transcript level of miRNA156 target SQUAMOSA promoter binding-like (SPL) transcription factors was suppressed severely in Cg1-expressing lines as compared to the wild type. Expression of glgC alone or in combination with Cg1 enhanced biomass yield and total sugar content per plant, suggesting the potential of these genes in improving economically important biofuel feedstocks. …”
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The effect of cell growth phase on the regulatory cross-talk between flagellar and Spi1 virulence gene expression. by Chakib Mouslim, Kelly T Hughes

“…Of six potential transcriptional start-sites within the flhDC promoter region, only two, P1(flhDC) and P5(flhDC), were functional in a wild-type background, while P6(flhDC) was functional in the absence of CRP. …”
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Ticam2 ablation facilitates monocyte exhaustion recovery after sepsis by Blake A. Caldwell, Susanti Ie, Amy Lucas, Liwu Li

“…After one week of recovery, we profiled bone marrow and splenic reservoir monocytes in Ticam2 -/- mice and found that, in contrast to the persistent exhaustion observed in wild-type monocytes, Ticam2 -/- monocytes largely resembled healthy controls. …”
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Role of CTCF protein in regulating FMR1 locus transcription. by Stella Lanni, Martina Goracci, Loredana Borrelli, Giorgia Mancano, Pietro Chiurazzi, Umberto Moscato, Fabrizio Ferrè, Manuela Helmer-Citterich, Elisabetta Tabolacci, Giovanni Neri

“…Here we demonstrate for the first time that rare unmethylated full mutation (UFM) alleles present the same boundary described in wild type (WT) alleles and that CTCF binds to this region, as well as to the FMR1 gene promoter, exon 1 and intron 2 binding sites. …”
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Strange features are no better than no features: predator recognition by untrained birds by Ondřej Fišer, Irena Strnadová, Petr Veselý, Michaela Syrová, Michal Němec, Barbora Kamišová, Josef Šalom, Roman Fuchs

“…We presented an unmodified kestrel dummy and two modified kestrel dummies (one with pigeon key features, the other lacking key features) to wild red-backed shrikes defending their nest. The shrikes attacked the unmodified dummy kestrel more intensively than both kestrel modifications when defending the nest. …”
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Abalones at risk: A global Red List assessment of Haliotis in a changing climate. by Howard Peters, Gina M Ralph, Laura Rogers-Bennett

“…In Japan, with a long history of abalone fishing, wild stocks are routinely supplemented with hatchery-bred juveniles. …”
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SURFACE PLASMON RESONANCE STUDY OF THE INTERACTION BETWEEN THE HUMAN TATA-BOX BINDING PROTEIN AND THE TATA ELEMENT OF THE NOS2A GENE PROMOTER by I. A. Drachkova, S. V. Shekhovtsov, S. E. Peltek, P. M. Ponomarenko, T. V. Arshinova, M. P. Ponomarenko, T. I. Merkulova, L. K. Savinkova, N. A. Kolchanov

“…The sensograms obtained in the experiment indicate that the constant for the interaction with TATA bearing SNP –21 t &gt; c differ little from the constant with the wild-type box and the dissociation constants differ approximately twofold. …”
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Analysis of Changes in Flavor Substances of Tomato Fruits in Different Ripening Stages by Headspace-Gas Chromatography-Ion Mobility Spectrometry by FU Xiaohui, SHI Lu, SUN Qingzhen, DONG Xiaoxing, PIAO Fengzhi, DU Nanshan

“…High performance liquid chromatography (HPLC) and headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) were employed to analyze the changes in volatile metabolites in wild-type Ailsa Craig tomato fruits in four ripening stages (mature green, breaker, light ripe and red ripe) at the metabolic and transcriptional levels. …”
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Superficial Dsg2 Expression Limits Epidermal Blister Formation Mediated by Pemphigus Foliaceus Antibodies and Exfoliative Toxins by Donna Brennan, Ying Hu, Walid Medhat, Alicia Dowling, Mỹ G. Mahoney

“…To further study the pathomechanism of these diseases and to assess the adhesive properties of Dsg2, we employed a recently established transgenic (Tg) mouse model expressing Dsg2 in the superficial epidermis. Neonatal Tg and wild type (WT) mice were injected with purified ETA or PF Ig. …”
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Application of DNA markers in molecular breeding and genetic studies of grapevine by E. T. Ilnitskaya, M. V. Makarkina

“…For this reason, the problem of a detailed study of the gene pool of the genus Vitis, wild populations and varieties created by men during the long cultivation history of this crop becomes more and more important every year. …”
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