Rational engineering of minimally immunogenic nucleases for gene therapy by Rumya Raghavan, Mirco J. Friedrich, Indigo King, Samuel Chau-Duy-Tam Vo, Daniel Strebinger, Blake Lash, Michael Kilian, Michael Platten, Rhiannon K. Macrae, Yifan Song, Lucas Nivon, Feng Zhang

“…SaCas9 and AsCas12a Redi variants are substantially less recognized by adaptive immune components, including reduced binding affinity to MHC molecules and attenuated generation of cytotoxic T cell responses, yet maintain wild-type levels of activity and specificity. In vivo editing of PCSK9 with SaCas9.Redi.1 is comparable in efficiency to wild-type SaCas9, but significantly reduces undesired immune responses. …”
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Evaluation of the Hepatoprotective Properties of Traditional Formulations Based on Cochlospermum tinctorium Used in Benin by Appolinaire K. Dossa, Jean Robert Klotoé, Victorien Dougnon, Eric Agbodjento, Rémi Akotègnon, Fréjus Ohouko, Manoir Hounkanrin, Kévine Vodounnon, Luc V. C. Brun, Fréderic Loko

“…The variables studied were the variety of C. tinctorium (wild form vs. cultivated form), the species associated with C. tinctorium (Combretum micranthum vs. …”
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Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides by Nai-Dong Chen, Nai-Fu Chen, Jun Li, Cai-Yun Cao, Jin-Mei Wang, He-Ping Huang

“…GC-MS method combined with FTIR techniques by the analysis of polysaccharide was applied to evaluate the similarity between wild (W) and tissue-cultured (TC) Dendrobium huoshanense (DHS), Dendrobium officinale (DO), and Dendrobium moniliforme (DM) as well as 3 wild Dendrobium spp.: Dendrobium henanense (DHN), Dendrobium loddigesii (DL), and Dendrobium crepidatum (DC). …”
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Potency of agricultural wastes in mushroom (Pleurotus sajor-caju) biotechnology for feeding broiler chicks (Arbor acre) by Charles Oluwaseun Adetunji, Isaac Oluseun Adejumo

“…The effect of dietary mycomeat produced from ogi production wastes based on yellow maize using wild and mutant strains of Pleurotus sajor-caju was assessed based on chickens’ growth, haematology and histology. …”
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Study of the effect of the introduction of mitochondrial import determinants into the gRNA structure on the activity of the gRNA/SpCas9 complex in vitro by E. G. Zakirova, Y. V. Vyatkin, N. A. Verechshagina, V. V. Muzyka, I. O. Mazunin, K. E. Orishchenko

“…The possible way to reduce adverse effects of the mutation is by shifting the level of heteroplasmy towards the wild-type mtDNA molecules. Using this idea, several gene therapeutic approaches based on TALE and ZF nucleases have been developed for this purpose. …”
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Hyaluronan Synthase 3 Null Mice Exhibit Decreased Intestinal Inflammation and Tissue Damage in the DSS-Induced Colitis Model by Sean P. Kessler, Dana R. Obery, Carol de la Motte

“…Most notable is the dramatic increase in submucosal microvasculature, hyaluronan deposition, and leukocyte infiltration in the inflamed colon tissue of wild-type and HAS1 null mice. Our data suggest, HAS3 plays a crucial role in driving gut inflammation. …”
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Individual and Society in Lu Xun's Works: A Journey from Tradition to Modernism by Ali Kiriktaş

“…Through his works Notes Under the Lamp and Wild Grass, Lu Xun delves deeply into the internal conflicts faced by individuals dealing with societal pressures and the process of modernization. …”
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Analysis of the Complete Genome Sequence of a Novel, Pseudorabies Virus Strain Isolated in Southeast Europe by Zsolt Csabai, Dóra Tombácz, Zoltán Deim, Michael Snyder, Zsolt Boldogkői

“…Conclusion. PRV-MdBio is a wild-type virus, which differs in base composition from other PRV strains to a relatively large extent.…”
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An analysis of the Medicago falcata s.l. alfalfas collected in the ex-USSR territories for the fullness of their coverage by N. Yu. Malysheva, L. L. Malyshev

“…Background. The collection of wild yellow alfalfas from the Medicago falcata s.l. complex maintained at the N.I. …”
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Do road movie a "viagem selvagem": a busca de aventura na estrada e o mito do viajante heróico by Pascale Argod

“…Os dois diários de viagem cinematográficos norte-americanos, « Into the Wild », de Sean Penn, de 2007, e « Wild », de Jean-Marc Vallée, de 2015, referem-se a On the Roadde Kérouac e a fronteira norte-americana e marcam uma geração de viajantes em busca de um ideal e da anti - viagem. …”
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Evaluating the representativeness of the bentgrass (Agrostis L.) gene pool collected across the Russian Federation and neighboring countries in the VIR collection by L. L. Malyshev

“…A. gigantea Roth is represented mainly by wild accessions; A. stolonifera L., A. canina L., and A. tenuis Sibth. by cultivars and breeding material of foreign origin. …”
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Morphological Diversity of Gracilaria blodgettii Harvey 1853 (Gracilariaceae, Rhodophyta) from Sarawak, Malaysian Borneo by Ruhana Hassan, Muhammad Nur Arif Othman, Mohd Nasarudin Harith, Amir Shah Ruddin Md Sah

“…This study was carried out to determine diversity of wild Gracilaria populations in Lawas, Santubong, and Asajaya, Sarawak, using the combination of morphological character examination and 5′ region of the mitochondrial cytochrome c oxidase 1 (CO1-5P) gene analysis. …”
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Experience in African swine fever control in the Russian Federation and its value for the other countries by K. N. Gruzdev, A. K. Karaulov, A. S. Igolkin

“…It occurred in Georgia for the first time in 2007 and spread among domestic pigs and wild boar in the European part of the Russian Federation. …”
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Regulator of Calcineurin 1 in Periodontal Disease by Ulrike Peters, Eleni Solominidou, Yüksel Korkmaz, Stefan Rüttermann, Astrid Klocke, Thomas Frank Flemmig, Thomas Beikler

“…HUVEC were incubated for 2 h with Vascular Endothelial Growth Factor (VEGF) or with wild type and laboratory strains of Porphyromonas gingivalis (P. gingivalis). …”
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Quails of Stavropol Region: Autumn Food Habits by Anatoly P. Kaledin, Lyubov V. Malovichko, Alexander G. Rezanov, Lyudmila S. Drozdova, Andiya T. Serikbayeva

“…In the autumn, they feeding on green plants, wheat grain, seeds of cultivated and wild plants, and insect larvae.…”
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Expression evaluation of exogenous and endogenous alcohol dehydrogenase genes in transgenic Arabidopsis by Haoqiang Yu, Hong Lv, Fengzhong Lu, Qingqing Yang, Huaming Duan, Wanchen Li, Fengling Fu

“…Enzyme reaction assay showed that the ADH activities of the T3 lines transformed by the exogenous genes ZmADH and EcADH were also significantly higher than that transformed by the endogenous gene AtADH as well as the wild-type control. These results indicated that exogenous genes were more conducive to transgenic improvement of crops.…”
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Interspecific hybridization of potato: theoretical and applied aspects by T. A. Gavrilenko, A. P. Yermishin

“…The most effective approach to broaden genetic diversity of breeding varieties is based on the introgression of genetic material of wild and cultivated species belonging to section Petota Dumort. of the genus Solanum L. …”
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Comparative effectiveness of liquid and freeze-dried vaccines for oral vaccination of dogs against rabies by Z. D. Yershebulov, A. T. Zhunushov, Zh. T. Amanova

“…Currently, vaccination is the main measure to combat rabies in domestic and wild animals. Inactivated and live vaccines are used for this purpose. …”
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Diacylglycerol kinase alpha regulates post-hepatectomy liver regeneration by Hiroki Nakamoto, Shunsuke Shichi, Chisato Shirakawa, Takuto Suzuki, Hidemitsu Kitamura, Akinobu Taketomi

“…Here, we provide a detailed examination of C57BL/6 wild-type and DGKα knockout (KO) mice subjected to 70% partial hepatectomy (70% PH) modeling, including survival rates, hematological marker and gene expression levels, and histological analyses of factors related to liver regeneration. …”
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Impaired Fas-Fas Ligand Interactions Result in Greater Recurrent Herpetic Stromal Keratitis in Mice by Xiao-Tang Yin, Tammie L. Keadle, Jessicah Hard, John Herndon, Chloe A. Potter, Chelsea R. Del Rosso, Thomas A. Ferguson, Patrick M. Stuart

“…We demonstrate that infection of the cornea with HSV-1 results in increased functional expression of FasL and that mice expressing mutations in Fas (lpr) and FasL (gld) display increased recurrent HSK following reactivation compared to wild-type mice. Furthermore, both gld and lpr mice took longer to clear their corneas of infectious virus and the reactivation rate for these strains was significantly greater than that seen with wild-type mice. …”
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