Two decades of occurrence of non-pathogenic rabbit lagoviruses in Italy and their genomic characterization by Patrizia Cavadini, Alice Vismarra, Francesca Merzoni, Vittoria Di Giovanni, Maria Beatrice Boniotti, Lorenzo Capucci, Antonio Lavazza

“…This study aimed firstly to indirectly show through a seroepidemiological survey from 1998 to 2008 the circulation of RCVs strains in rabbit farms and then to genetically characterise RCV strains diagnosed in Italy in faecal and intestinal samples of wild and farmed rabbits collected in various regions in the following years (2000–2022). …”
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Development and Evaluation of the Immunogenic Potential of an Unmodified Nucleoside mRNA Vaccine for Herpes Zoster by Shun Zhang, Xiaojie Wang, Tongyi Zhao, Chen Yang, Lulu Huang

“…Results: Codon-optimized mRNA sequences showed significantly higher protein expression levels in vitro compared to wild-type (WT) sequences. Vaccination with Vac07 demonstrated immunogenicity in mice that was comparable to, or even superior to, the licensed Shingrix vaccine, characterized by a stronger Th1-biased antibody response and a slightly more robust Th1-type cellular response. …”
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BvBZR1 improves parenchyma cell development and sucrose accumulation in sugar beet (Beta vulgaris L.) taproot by Ningning Li, Wei Wang, Wei Wang, Xiaotong Guo, Yaqing Sun, Guolong Li, Shaoying Zhang

“…Transgenic sugar beet overexpressing BvBZR1 exhibited a higher taproot diameter compared with the wild type, mainly due to a significant enhancement in the spacing between cambial rings by increasing the size and layers of parenchyma cells. …”
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‘Customary’ use of stick tools by chimpanzees in Bulindi, Uganda: update and analysis of digging techniques from behavioural observations by Matthew R. McLennan, Jacqueline Rohen, Zoe Satsias, Tom Sabiiti, John-Mary Baruzaliire, Marie Cibot

“…Wild chimpanzees commonly use stick tools in foraging, often to harvest honey and other insect foods. …”
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Efficient biosynthesis of β-caryophyllene by engineered Yarrowia lipolytica by Young-Kyoung Park, Lucie Studena, Piotr Hapeta, Ramdane Haddouche, David J. Bell, Pablo Torres-Montero, Jose Luis Martinez, Jean-Marc Nicaud, Adriana Botes, Rodrigo Ledesma-Amaro

“…Employing the same combination of promoters and genes in wild-type-based strain with two copies resulted in a 1.36-fold increase in β-caryophyllene. …”
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A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental Caries by Wenting Pan, Tiantian Mao, Qing-an Xu, Jin Shao, Chang Liu, Mingwen Fan

“…Adhesion assay showed that the adhesion ability of the mutant increased relative to the wild type, especially in the early stage. MS-gcrR-def out-competed S. mutans UA159 in vitro biofilm, and correspondingly coinfection displayed significantly fewer caries in vivo. …”
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Von Hippel-Lindau (VHL) inactivation in sporadic clear cell renal cancer: associations with germline VHL polymorphisms and etiologic risk factors. by Lee E Moore, Michael L Nickerson, Paul Brennan, Jorge R Toro, Erich Jaeger, Jessica Rinsky, Summer S Han, David Zaridze, Vsevolod Matveev, Vladimir Janout, Hellena Kollarova, Vladimir Bencko, Marie Navratilova, Neonilia Szeszenia-Dabrowska, Dana Mates, Laura S Schmidt, Petra Lenz, Sara Karami, W Marston Linehan, Maria Merino, Stephen Chanock, Paolo Boffetta, Wong-Ho Chow, Frederic M Waldman, Nathaniel Rothman

“…Risk of having genetic VHL inactivation was inversely associated with smoking due to a higher proportion of wild-type ccRCC tumors [former: OR = 0.70 (0.20-1.31) and current: OR = 0.56 (0.32-0.99); P-trend = 0.04]. …”
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Cryopreservation of epididymal semen of domestic cat by S. Ya. Amstislavsky, E. Yu. Brusentsev, V. I. Mokrousova, E. A. Kizilova, V. V. Kozhevnikova, V. A. Naprimerov, I. N. Rozhkova

“…Domestic cat (Felis silvestris catus) is used as a model species for developing effective methods of wild felids’ semen cryopreservation. The present study represents a comparison of domestic cat semen cryopreservation with two commercially available cryoprotectant agents (CPAs): CaniPlus Freeze (CPF) and SpermFreeze (SF). …”
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Experimental Aerosol Inoculation and Investigation of Potential Lateral Transmission of Mycobacterium bovis in Virginia Opossum (Didelphis virginiana) by Karla A. Fenton, Scott D. Fitzgerald, Steve Bolin, John Kaneene, James Sikarskie, Rena Greenwald, Konstantin Lyashchenko

“…The objective of this study was to determine if Virginia opossums (Didelphis virginiana) contribute to disease persistence by experimentally assessing intraspecies lateral transmission. One wild caught pregnant female opossum bearing 11 joeys (young opossum) and one age-matched joey were obtained for the study. …”
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Substrate Specificity and Enzymatic Characteristics of Three Rutinosidases by Fengwei XIAO, Jiang SUN, Jiaying YE, Lijun LI, Hui NI

“…Additionally, 10 mmol/L β-ME and DTT significantly enhanced AnRut's enzymatic activity, increasing the relative activity to 223% and 242% of the wild type, respectively. The optimum temperature and pH of αRβD I was 70 ℃ and 4.0, demonstrating efficient hydrolysis of narirutin and hesperidin under acidic conditions. αRβD II, meanwhile, had an optimal temperature of 40 ℃ and an optimal pH of 6.0, indicating its suitability for hydrolyzing rutinoside flavonoids under neutral conditions. …”
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The relationship between bats and human coronavirus: An exploratory review by Sajal BHATTACHARYA, Shakya SINHA, Rina TILAK, Sugeng Juwono MARDIHUSODO

“…Anthropogenic activities like encroachment of wildlife habitat, deforestation, developmental activities and close encounter with wild animals, cause disturbances in ecosystem functioning. …”
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Allele-specific PCR with fluorescently labeled probes: criteria for selecting primers for genotyping by V. A. Devyatkin, A. A. Shklyar, A. Zh. Fursova, Yu. V. Rumyantseva, O. S. Kozhevnikova

“…The method consists of real-time PCR with a pair of primers and two oligonucleotide probes that are complementary to a sequence near a given locus in such a way that one probe is complementary to the wild-type allele, and the other to a mutant one. …”
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Normalization of Cystic Fibrosis Immune System Reverses Intestinal Neutrophilic Inflammation and Significantly Improves the Survival of Cystic Fibrosis MiceSummary by Callie E. Scull, Yawen Hu, Scott Jennings, Guoshun Wang

“…Methods: CF and sibling wild-type (WT) mice underwent reciprocal bone marrow transplantation. …”
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Fecal microbiota transplantation attenuates Alzheimer’s disease symptoms in APP/PS1 transgenic mice via inhibition of the TLR4-MyD88-NF-κB signaling pathway-mediated inflammation... by Xiang Li, Qingyong Ding, Xinxin Wan, Qilong Wu, Shiqing Ye, Yongliang Lou

“…In this study, FMT was performed by transplanting fecal microbiota from healthy wild-type mice into APP/PS1 mice (APPswe, PSEN1dE9) to assess the effectiveness of FMT in mitigating AD-associated inflammation and to reveal its precise mechanism of action. …”
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Effects of Dietary Protein to Lipid Ratio and Insect Meal on Growth Performance, Feed Utilization, and the Gut Microbiome of Lake Whitefish (Coregonus clupeaformis) by Rebecca Lawson, Yubing Chen, Junyu Zhang, Marcia A. Chiasson, Jennifer Ellis, Dominique Bureau, Richard D. Moccia, David Huyben

“…Wild stocks of lake whitefish (Coregonus clupeaformis) are declining in the Great Lakes, and there is a lack of information on their nutritional requirements and gut health indicators to effectively culture them in an aquaculture setting. …”
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Engineering of Dihydroxy Biphenyl Dioxygenase Enzyme to Improve Its Performance In Removing PCBs: A Molecular Docking Study by Susan Yazdankhah, Alireza Zakeri, Saeed Khalili, Abolfazl Jahangiri, ZahraalSadat Hashemi, Alireza Heidari

“…Results: By molecular docking with PYRX software between the wild enzyme and the substrate, the binding energy -6.2 Kcalmol-1and for the candidates of mutations resulting from the alignment, Phenylalanine 201 to Threonine (6. 9 Kcalmol-1) and Threonine 280 to serine (6. 8 Kcalmol-1) Calculated. …”
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An increased proportion of transgenic plants in the progeny of rapeseed (<i>Brassica napus</i> L.) transformants by G. N. Raldugina, T. Z. Hoang, H. B. Ngoc, I. V. Karpichev

“…Based on these results, we developed a simplified method, which consists of several rounds of a combination of cutting, seed production by self-pollination, and subsequent culling of wild-type plants, which significantly enriched descendent populations of the original rapeseed transformants with plants transgenic for the gfp marker.…”
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Ecological drivers of evolution of swine influenza in the United States: a review by Varun Goel, Jessica Ding, Bijaya Hatuwal, Emily Giri, Thomas J. Deliberto, James Lowe, Richard Webby, Michael Emch, Xiu-Feng Wan

“…Additionally, interactions at various interfaces, such as between commercial swine and feral swine, humans, or wild birds contribute to the increase in genetic diversity of swine IAVs. …”
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Genetic Instability Promotes the Acquisition of Chromosomal Imbalances in T1b and T1c Breast Adenocarcinomas by Harald Blegen, B. Michael Ghadimi, Annukka Jauho, Anders Zetterberg, Elina Eriksson, Gert Auer, Thomas Ried

“…The results show that T1b carcinomas (6–10 mm, n=17) were frequently near‐diploid (53%) with low proliferative activity and staining patterns of p53 and p21WAF1 that suggest the presence of wild type protein. The majority (12/16) of the T1c tumors (11–20 mm), however, was aneuploid, and proliferative activity and p53 expression were increased. …”
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Linking altered neuronal and synaptic properties to nicotinic receptor Alpha5 subunit gene dysfunction: a translational investigation in rat mPFC and human cortical layer 6 by Danqing Yang, Guanxiao Qi, Daniel Delev, Uwe Maskos, Dirk Feldmeyer

“…We performed single cell-electrophysiology combined with morphological reconstructions on layer 6 (L6) excitatory neurons in the medial PFC (mPFC) of wild type (WT) rats, rats carrying the human coding polymorphism rs16969968 in Chrna5 and α5 knockout (KO) rats. …”
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