Characteristics of Nanocrystallite-CdS Produced by Low-Cost Electrochemical Technique for Thin Film Photovoltaic Application: The Influence of Deposition Voltage by Obi Kingsley Echendu, Francis Birhanu Dejene, Imyhamy Mudiy Dharmadasa, Francis Chukwuemeka Eze

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Diversity and antibacterial potential produced by marine endophytic fungi by submerged fermentation from Buton Island, Indonesia by Isti’anah Ismi, Tarman Kustiariyah, Heri Suseno Sugeng, Nugraha Roni, Effendi Irzal, Lee Wen-Chien, Lukitaningsih Endang, Manggau Marianti A., Sari Rodiah Nurbaya

“…This study focused on identifying fungi capable of producing bioactive compounds effective against Vibrio harveyi. 32 fungal isolates were obtained from various marine samples. Aspergillus terreus (WB 1-2) exhibited the highest antibacterial activity. …”
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RNA binding protein ALYREF regulates ferroptosis to facilitate LUAD growth and metastasis via promoting SLC7A11 mRNA stability by Yanni Chen, Ting Zhao, Hao Chen, Jun Chen, Weili Han

“…In this study, we discovered by WB and IHC that ALYREF has a higher expression in lung adenocarcinoma (LUAD) tissues compared with normal ones. …”
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Study on the role of activation of TGF-β pathway by ASPN in the occurrence and development of keloid and the underlying mechanisms by XU Xueyan, CHEN Junyi, YAO Liuyi, DENG Chengcheng

“…[Methods] Immunofluorescence staining was used to assess the expression levels of ASPN in keloid of keloid patients and normal scar. WB and qRT-PCR were used to assess the activity of TGF-β pathway and expression levels of α-SMA、Collagen Ⅰ/Ⅲ, COMP and POSTN in fibroblasts with overexpression of ASPN, ASPN-treated fibroblasts and Keloid fibroblasts with ASPN knockdown. …”
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Neurological damages in COVID‐19 patients: Mechanisms and preventive interventions by Sibani Sarkar, Subhajit Karmakar, Malini Basu, Pratyasha Ghosh, Mrinal K Ghosh

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Identification and Validation of Pivotal Genes in Osteoarthritis Combined with WGCNA Analysis by Yang C, Chen X, Liu J, Wang W, Sun L, Xie Y, Chang Q

“…Functional enrichment analyses, protein-protein interaction (PPI) analysis, and weighted gene co-expression network analysis (WGCNA) of the DEGs were performed. RT-qPCR and WB were further performed to verify the hub gene expression in OA rat.Results: In this study, 35 key genes were identified through differential expression analysis and weighted gene co-expression network analysis (WGCNA) using the GSE169077 and GSE114007 datasets. …”
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Immunofluorescence for Detection of TOR Kinase Activity In Situ in Photosynthetic Organisms by Ana Lando, María De Marco, Andrea Cumino, Giselle Martínez-Noël

“…Protein phosphorylation levels are measured by a western blot (WB) technique using an antibody against one specific phosphosite in cell extracts. …”
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PPAR-α regulates metabolic remodelling and participates in myocardial fibrosis in patients with atrial fibrillation of rheumatic heart disease by Xiaoying Hu, Daisong Jiang, Zheng Zhang, Zhenmei An

“…Results Masson staining showed that the degree of myocardial fibrosis in patients with AF was significantly increased; WB analysis showed that there were statistically significant differences in protein expression related to glucose metabolism disorder, lipid metabolism abnormality, and mitochondrial dysfunction. …”
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Antimicrobial susceptibility, biofilm formation, and virulence genes among atypical enteropathogenic Escherichia coli stool isolates in Tehran, Iran by Shahnaz Halimi, Akram Rezaei, Shirin Mohebi, Farhad Bonakdar Hashemi

“…About 61% of isolates produced Moderate Biofilm (MB), and the frequency of Weak Biofilm (WB) formers (27%) was higher among D and ND isolates, which carried virulence genes more frequently than D isolates. …”
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First molecules to reactivate RASG12V GTPase activity by Aneta Wlodarczyk, Cezary Treda, Marcin Pacholczyk, Adrianna Rutkowska, Marta Wegierska, Amelia Kierasinska-Kalka, Katarzyna Wasiak, Damian Ciunowicz, Dagmara Grot, Pawel Glowacki, Ewelina Stoczynska-Fidelus, Piotr Rieske

“…Efficacy was verified based on the IC50 determination, GTPase activity, as well as the AKT and ERK phospho WB assays. Results The IC50 of the tested compounds was significantly lower against cells with the RASG12V mutation than against selected types of normal cells. …”
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Blockade of Vascular Endothelial Growth Factor Receptor 1 Prevents Inflammation and Vascular Leakage in Diabetic Retinopathy by Jianbo He, Hong Wang, Ying Liu, Wen Li, Dorothy Kim, Hu Huang

“…Protein expression and localization were examined by western blots (WB) and immunofluorescence (IF). mRNA expression was quantified by PCR array and real-time PCR. …”
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Developmental exposure to perfluorooctane sulfonate(PFOS) impairs the endometrial receptivity by Rui Ren, Xinyue Zhou, Tianyu Jia, Bin Wang, Ahui Liu, Min Gao, Ji Song, Liyan Wang, Yuanxue Jing, Liulin Yu, Haofei Shen, Xuehong Zhang

“…Transmission electron microscopy results revealed mitochondrial damage in the PFOS-intervened group, and WB results showed that the expression levels of endometrial tolerance-related proteins Homeobox A10 (HOXA10) and integrin beta3 (ITGB3) were decreased, and the expression level of Forkhead box O1 (FOXO1) protein was increased. …”
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A Theoretical Study on the Influence of the Functional Group Electronic Effect on the Electron Mobility of Cross-Linked Polyethylene by Yang Du, Hui Zhang, Xia Du, Yan Shang, Xuan Wang, Qingguo Chen, Zesheng Li

“…Compared with the experimental values, the theoretical calculation results show that wB97XD/6-311G(<i>d</i>,<i>p</i>) is more suitable for studying the electron mobility system. …”
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Symmetric Dimethylarginine Is Not Associated with Cumulative Inflammatory Load or Classical Cardiovascular Risk Factors in Rheumatoid Arthritis: A 6-Year Follow-Up Study by Theodoros Dimitroulas, James Hodson, Aamer Sandoo, Jacqueline P. Smith, Karen M. Douglas, George D. Kitas

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Performance evaluation of diminutive solar dryer for drying of green coffee beans: In Ethiopian highlands by Addisu Yenesew Kebede, Muluken Temesgen Tigabu, Asmare Tezera Admase, Atrsaw Jejaw Bezie

“…Nevertheless, the new dryer could reduce the moisture content of coffee beans from 60 % to 10 % on a wet basis (W.B.) within a couple of days. This is mainly reducing the energy and time wasted by natural drying system to dry coffee beans. …”
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The Neuroprotective Effects of Astragaloside IV against H2O2-Induced Damage in SH-SY5Y Cells are Associated with Synaptic Plasticity by Zurong Song, Ali Tao

“…The expression of postsynaptic density 95 (PSD-95) and growth-associated protein 43 (GAP-43) were measured by western blot (WB) and inmunofluorescence staining assay under the same treatment conditions. …”
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Study on the protective effect of mitochonic acid-5 on septic acute kidney injury and the underlying mechanism by Li Peng-yan, Yan miao, Yang Ding-ping

“…方法细胞实验：将肾小管细胞分为正常组、MA-5（10 µmol/L）组、LPS（10 mg/L）刺激组和LPS+MA-5治疗组，通过细胞计数试剂盒8法评估细胞活力、流式细胞术评估细胞凋亡率；活性氧（reactive oxygen species，ROS）检测试剂盒检测ROS含量；三磷酸腺苷（adenosine triphosphate，ATP）检测试剂盒检测ATP水平；蛋白质印迹法（western blot，Wb）检测沉默信息调节因子3（silence information regulator 3，SIRT3）蛋白、线粒体动力学相关蛋白［动力相关蛋白1（dynamin-related protein 1，DRP1）、视神经萎缩因子1（optic atrophy 1，OPA1）］和细胞凋亡相关蛋白［B细胞淋巴瘤2（B-cell lymphoma-2，Bcl-2）、Bcl-2相关X蛋白（Bcl-2 associated X protein，Bax）］的表达水平。…”
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Downregulation of FXYD2 Is Associated with Poor Prognosis and Increased Regulatory T Cell Infiltration in Clear Cell Renal Cell Carcinoma by Zedan Zhang, Yanlin Tang, Lei Li, Wuping Yang, Yawei Xu, Jingcheng Zhou, Kaifang Ma, Kenan Zhang, Hongkai Zhuang, Yanqing Gong, Kan Gong

“…FXYD2 was identified to be downregulated in ccRCC tissue compared to normal tissue, which was confirmed by our RT-PCR, WB, and IHC analyses. Kaplan-Meier survival analysis and Cox regression analysis suggested that downregulated FXYD2 could independently predict poor survival of ccRCC patients. …”
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Synergistic Antitumor Effects of Anlotinib Combined with Oral 5-Fluorouracil/S-1 via Inhibiting Src/AKT Signaling Pathway in Small-Cell Lung Cancer by Xinhang Xia, Wenhu Pi, Yanli Lan, Xiaomai Wu, Dongqing Lv, Yinnan Meng, Haihua Yang, Wei Wang

“…Body weights and tumor sizes were recorded. WB was conducted using tumor tissues. All data were collected and statistically analyzed using t-test to reveal the underlying molecular mechanism. …”
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Comprehensive analysis of the prognostic, immunological, and diagnostic roles of SIRT1 in pan-cancer and its validation in KIRC by Qi Liu, Songxian Sun, Chunxiang Zhou, Houxi Xu, Houxi Xu

“…Quantitative reverse transcription polymerase chain reaction (qRT–PCR) and Western blot (WB) analyses confirmed that SIRT1 is differentially expressed in kidney renal clear cell carcinoma (KIRC).ConclusionsUsing an integrative approach involving bioinformatics analysis and experimental validation, we clarified the potential roles and mechanisms of SIRT1 in pan-cancer, providing a theoretical basis for the development of SIRT1-targeted therapies in clinical applications.…”
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