The Mutual Inhibition of FoxO1 and SREBP-1c Regulated the Progression of Hepatoblastoma by Regulating Fatty Acid Metabolism by Yu Hu, Hongyan Zai, Wei Jiang, Zhenglin Ou, Yuanbing Yao, Qin Zhu

“…FoxO1, SREBP-1c, FASN, ACLY, ACC, and MAGL expressions in tissue samples were detected by RT-qPCR and WB. IHC was utilized to measure FASN content. Overexpression and knockdown of FoxO1 and sSREBP-1c were performed on Huh-6 cells. …”
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Effect of intramuscular fat level on carcass composition, physicochemical characteristics, texture, and microstructure of breast muscle of broiler chickens by Dariusz Kokoszyński, Karol Włodarczyk, Joanna Żochowska-Kujawska, Marek Kotowicz, Marcin Wegner, Kamil Stęczny, Dorota Cygan-Szczegielniak

“…To date, there have been no studies determining the effect of IMF level on microstructure and texture traits of m. pectoralis major (except WB shear force) and electrical conductivity in Ross 308 broiler chickens. …”
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Rhopalurus junceus scorpion venom induces G2/M cell cycle arrest and apoptotic cell death in human non-small lung cancer cell lines by Alexis Díaz-García, Ángel Garrido, Jenny Laura Ruiz-Fuentes, Tamara Hermosilla, Diego Varela

“…The effects on cell cycle, apoptosis, and cell signaling-related proteins were determined by flow cytometry and WB. Protein fractions responsible for the observed effect were identified using HPLC. …”
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Mechanism of mTOR/RILP-regulated autophagic flux in increased susceptibility to myocardial ischemia-reperfusion in diabetic mice by Jiyao Zhao, Wei Shi, Yan Zheng, Junjie Wang, Muzhao Yuan, Yultuz Anwar, Yuxuan He, Haiping Ma, Jianjiang Wu

“…Transmission electron microscopy revealed that the accumulation of autophagic vesicles in cardiomyocytes of diabetic mice resulted in impaired autophagic flux. qRT-PCR revealed that the expression of RILP was significantly reduced in diabetic mice subjected to I/RI. WB showed that P62 was significantly increased and RILP was significantly decreased in diabetic mice subjected to I/RI compared to healthy mice. …”
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WNT3A promotes the cementogenic differentiation of dental pulp stem cells through the FOXO1 signaling pathway by Dongmei Cheng, Yang Bao, Xue Wang, Haidong Xiang, Tianyuan Guo, Yong Du, Zhiyong Zhang, Han Guo

“…Changes in protein expression related to the FOXO1 signaling pathway, cell cycle, and apoptosis were evaluated using western blotting (WB). Results Differentially expressed genes (DEGs) associated with DPSC cementogenic differentiation were predominantly enriched in crucial pathways such as the signaling pathway, cell apoptosis, and Wnt signaling pathway. …”
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NCAPD3‐mediated AKT activation regulates prostate cancer progression by Yi Zhang, Wanlin Xie, Xicui Zong, Yuanyuan Fang, Jia Ren, Zuolei Jing, Yong Wei, Shan Lu, Qingyi Zhu, Ping Liu

“…Clinical samples and cell lines were used to measure the expression of genes by quantitative real‐time RT‐PCR (qRT‐PCR), Western‐blot assay (WB), immunohistochemistry (IHC), and immunofluorescence (IF). …”
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Trimetazidine: Activating AMPK Signal to Ameliorate Coronary Microcirculation Dysfunction after Myocardial Infarction by Xiaolong Qu, Pan Yang, Li Jiao, Yuehui Yin

“…Griess reaction method, immunofluorescence, and western blotting (WB) were employed to detect the expressions of nitric oxide (NO), platelet endothelial cell adhesion molecule-1 (CD31) and vascular endothelial (VE)-cadherin, zonula occludens protein 1 (ZO-1), occludin, vascular endothelial growth factor (VEGF) and adenosine monophosphate (AMP)-activated protein kinase (AMPK) signaling-related proteins in endothelial cells and mouse cardiomyocytes. …”
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Analysis of health expenditures in China from 2000 to 2019 compared with the world and upper-middle-income countries by Xindian Zeng, Xindian Zeng, Lijie Chen, Lu Chen

“…The goal is to provide a theoretical basis for the Chinese government to adjust and formulate health-related policies.MethodsAggregate time-series data were collected from the World Health Organization (WHO) and the World Bank (WB) Open Data sources from 2000 to 2019. These data were compared and some of it analyzed using cluster analysis methods.ResultsThe financing structure level of THE in China is currently above average among upper-middle-income countries. …”
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miR-21 Overexpression Promotes Esophageal Squamous Cell Carcinoma Invasion and Migration by Repressing Tropomyosin 1 by Zhuojian Shen, Xia Xu, Liangzhan Lv, Honglue Dai, Ju Chen, Baishen Chen

“…MiR-21 and TPM1 expressions were analyzed by RT-qPCR and WB in 30 ESCC, 10 lung cancer, and 10 bladder cancer clinical specimens, each with matched adjacent normal tissue. …”
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Exploring and Verifying the Mechanism and Targets of Shenqi Pill in the Treatment of Nonalcoholic Steatohepatitis via Network Pharmacology and Experiments by Beihui He, Zheng Chen, Yunmeng Nie, Minmin Luo, Sumei Xu, Junbin Yan, Zhiyun Chen

“…Hepatic injury and lipid metabolism indicators of NASH rats were significantly improved after SQP treatment. The results of WB and qRT–PCR showed that the expression of PTGS2, JUN, MYC, and CDKN1A was higher in NASH rats than in normal rats and decreased after SQP treatment. …”
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The in vitro anticancer effects of FS48 from salivary glands of Xenopsylla cheopis on NCI-H460 cells via its blockage of voltage-gated K+ channels by Xiong Weichen, Fan Huizhen, Zeng Qingye, Deng Zhenhui, Li Guanhui, Lu Wancheng, Zhang Bei, Lai Shian, Chen Xin, Xu Xueqing

“…The present study aims to reveal the effects of FS48 on the Kv channels and the NCI-H460 human lung cancer cells through patch clamp, MTT, wound healing, transwell, gelatinase zymography, qRT-PCR and WB assays. The results demonstrated that FS48 can be effective in suppressing the Kv currents, migration, and invasion of NCI-H460 cells in a dose-dependent manner, despite the failure to inhibit the proliferation. …”
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Metformin Ameliorates Ulcerative Colitis Through Inhibiting NLRP3 Inflammasome Activation by Cao R, Jing J, Ma Y, Qi W, Huang X, Zhang C, Lu Z, He J, Wang G, Ma Y, Zhang H

“…Enzyme-linked immunosorbent assay (ELISA), Reverse transcription - quantitative polymerase chain reaction (RT-qPCR), Western blotting (WB), immunohistochemistry, and immunofluorescence were used to detect NLRP3 inflammasome activation in vivo. …”
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Suppressing GDF15 enhances the chemotherapeutic effect of 5 FU on MSI-H CRC by regulating the ferroptosis pathway SLC7A11/GSH/GPX4 by Zhi min Huang Fu, Ming Xiao, Hailun Xie, Shuxian Zhang, Tang Yi, Qingshu Li, Ming Li, Yalan Wang

“…In our study, we found that GDF15 is generally elevated in pancarcinoma, particularly in colorectal cancer, and serves as an early indicator of the development of colorectal cancer. IHC and WB confirmed that GDF15 was elevated in MSI-H CRC clinical tissues and MSI-H CRC cell lines (HCT-116 and LoVo). …”
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Dose-Weighted Network Pharmacology: Evaluating Traditional Chinese Medicine Formulations for Lumbar Disc Herniation by Zhou C, Xiang T, Yu Y, Ma H, Liu C, Yang F, Yang L

“…TSD, YJD, and PQT were evaluated in animal experiments, and the algorithm’s feasibility was validated through GO and KEGG pathway analysis, Thermal Hyperalgesia Test, H&E staining, Western blotting (WB), RT-PCR, and ELISA assays.Results: The computational model indicated that YJD and PQT had higher predicted efficacy compared to TSD. …”
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Novel method for classification of prion diseases by detecting PrPres signal patterns from formalin-fixed paraffin-embedded samples by Sachiko Koyama, Kaoru Yagita, Hideomi Hamasaki, Hideko Noguchi, Masahiro Shijo, Kosuke Matsuzono, Kei-Ichiro Takase, Keita Kai, Shin-Ichi Aishima, Kyoko Itoh, Toshiharu Ninomiya, Naokazu Sasagasako, Hiroyuki Honda

“…Prion disease is an infectious and fatal neurodegenerative disease. Western blotting (WB)-based identification of proteinase K (PK)-resistant prion protein (PrPres) is considered a definitive diagnosis of prion diseases. …”
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Electroacupuncture Pretreatment Elicits Tolerance to Cerebral Ischemia/Reperfusion through Inhibition of the GluN2B/m-Calpain/p38 MAPK Proapoptotic Pathway by Bao-yu Zhang, Guan-ran Wang, Wen-hua Ning, Jian Liu, Sha Yang, Yan Shen, Yang Wang, Meng-xiong Zhao, Li Li

“…Model preparation for MCAO/R or sham MCAO/R started 2 hours after the last pretreatment. 24 hours after model preparation, the Garcia neurobehavioral scoring criteria was used for the evaluation of neurological deficits, TTC for the measurement of infarct volume, TUNEL staining for determination of neural cell apoptosis at hippocampal CA1 area, and WB and double immunofluorescence staining for expression and the cellular localization of GluN2B and m-calpain and p38 MAPK. …”
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Comparative Evaluation and Measure of Accuracy of ELISAs, CLIAs, and ECLIAs for the Detection of HIV Infection among Blood Donors in China by Le Chang, Junpeng Zhao, Fei Guo, Huimin Ji, Lu Zhang, Xinyi Jiang, Lunan Wang

“…All plasma samples were tested by eight ELISA assays in 16 blood centers, followed by the detection of CLIA and ECLIA methods in the National Center for Clinical Laboratories (NCCL), further confirmed by nucleic acid testing (NAT) and Western blot (WB). Results. Of 1029 samples, 136 were confirmed as HIV positive. …”
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Effects of environmental bisphenol S exposure on male rat reproductive health and gut-blood-testicular axis integrity by Biran Zhu, Zequan Zhang, Ying Xie, Min Huang, Yu Chen, Yong Yang, Xiongjie Shi, Jian Han, Lihua Yang, Min Zhao

“…Furthermore, histopathological analysis, western blot (WB), enzyme-linked immunosorbent assay (ELISA), and immunofluorescence results showed that exposure to BPS led to testicular damage, inflammation, activation of the p38 and ERK MAPK pathways, and disruption of the blood-testis barrier (BTB). …”
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Deciphering the potential ability of DExD/H-box helicase 60 (DDX60) on the proliferation, diagnostic and prognostic biomarker in pancreatic cancer: a research based on silico, RNA-... by Dongdong Zhang, Enze Zhang, Ying Cai, Yixin sun, Peiji Zeng, Xiaohua Jiang, Yifan Lian

“…First, we constructed the plasmid and tested the plasmid effect by WB. Then MTT assay was performed to explore the effect of DDX60 knockout on the proliferation of pancreatic cancer cells. …”
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Protective Effects of Baicalein on Lipopolysaccharide-Induced AR42J PACs through Attenuation of Both Inflammation and Pyroptosis via Downregulation of miR-224-5p/PARP1 by Ming-Wei Liu, Chun-Hai Zhang, Shou-Hong Ma, De-Qiong Zhang, Li-Qiong Jiang, Yang Tan

“…The RNA and protein expression of miR-224-5p, poly ADP-ribose polymerase-1 (PARP1), nuclear transcription factor-κB65 (NF-κB65), phospho-kappa B alpha(p-IκB-α), interleukin(IL)-18R, NOD-like receptor thermal protein domain-associated protein 3 (NLRP3), gasdermin D (GSDMD), apoptosis-associated speck-like protein containing a CARD (ASC), and caspase-1 was detected based on the WB and RT-PCR assays. IL-1β, IL-6, IL-18, and TNF-α expression levels in AR42J cells were measured via ELISA method. …”
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