A novel de novo GABRA2 gene missense variant causing developmental epileptic encephalopathy in a Chinese patient by Li Yang, Xingyu Wan, Ran Hua, Junhong Jiang, Baotian Wang, Rui Tao, De Wu

“…A mutant plasmid was constructed and transfected into 293 T cells. Western blotting (WB) was used to evaluate mutant protein expression, while co‐immunoprecipitation (Co‐IP) analyzed interactions with GABRB3 and GABRG2 proteins. …”
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Biological activity produced by ruthenium chloride on perfusion pressure, left ventricular pressure and heart failure using an isolated rat heart model by Figueroa-Valverde Lauro, Rosas-Nexticapa Marcela, Alvarez-Ramirez Magdalena, Lopez-Ramos Maria, Aguilar-Sanchez Emilio, Mateu-Armand Virginia

“…Results: The results showed that ruthenium chloride increased the perfusion pressure from 1 to 100 nM; ruthenium chloride increased LVP in a dose-dependent manner, whose effect was inhibited by prazosin and WB-4101; and the ruthenium derivative significantly decreased the infarct area (p = 0.05) compared with the control conditions. …”
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Anomalous tqγ Couplings in γp Collision at the LHC by M. Köksal, S. C. İnan

“…We have examined the constraints on the anomalous tqγ  (q=u,c) couplings through the process pp→pγp→pWbX at the LHC by considering four forward detector acceptances: 0.0015<ξ<0.5, 0.0015<ξ<0.15, 0.015<ξ<0.15, and 0.1<ξ<0.5, where ξ=Eγ/E with Eγ and E the energies of the photon and of the incoming proton, respectively. …”
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Inhibition of id-1 reduces osteosarcoma growth and metastasis through mediation of snail by Rongbing Shu, Zhuanyi Yu, Jianmin Wu, Qiuxin Cheng, Zhihao Peng, Huaqiang Zhou, Min Zhao

“…Immunohistochemistry and WB results showed upregulation of E-cadherin and downregulation of vimentin, N-cadherin, Id-1, and Snail in the sh-Id-1 group (P < 0.05). …”
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Preference for Efficiency or Confusion? A Note on a Boundedly Rational Equilibrium Approach to Individual Contributions in a Public Good Game by Luca Corazzini, Marcelo Tyszler

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Robust and Unbiased Variance of GLM Coefficients for Misspecified Autocorrelation and Hemodynamic Response Models in fMRI by Lourens Waldorp

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TBRs, a methodology for the multi-scalar cartographic analysis of the distribution of plant formations by Rafael Cámara Artigas, Fernando Díaz del Olmo, Jose Ramon Martinez Batlle

“… An analytical and cartographic method of biomass distribution and plant formations at a multi-scalar level is developed based on bioclimatic variables extracted from the Thornthwaite Water Balance (WB) and the Bioclimatic Balances (BB) of Montero de Burgos & González Rebollar. …”
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Gene Therapy for Parkinson's Disease by Rachel Denyer, Michael R. Douglas

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Systemowe ujęcie biosfery w etyce Edwarda Goldsmitha by Anita Ganowicz-Bączyk

“…Margulis Gaia’s hypothesis, the views of E. Odum, W.B. Cannon and C.H. Waddington. Then the main points of biospheric ethics are presented. …”
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Design and Development of a Vertical Paddle Semi Automated Garri Frying Machine by C.P. Nwadinobi, J.C. Edeh, K.I. Mejeh

“…In addition, a preferred final moisture content of 12.14%w.b. was achieved at 20rpm paddle speed and frying time of 23 minutes. …”
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Design and Development of a Vertical Paddle Semi Automated Garri Frying Machine by C.P. Nwadinobi, J.C. Edeh, K.I. Mejeh

“…In addition, a preferred final moisture content of 12.14%w.b. was achieved at 20rpm paddle speed and frying time of 23 minutes. …”
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αAsarone alleviates neuronal injury by facilitating autophagy via miR-499-5p/PDCD4/ATG5 signaling pathway in ischemia stroke by Yonghuan Yan, Linfang Wu, Lu Wang, Dandan Wang, Mengting Huang, Jinyong Peng, Jinyong Peng, Yingying Huang, Yingying Huang, Yingying Huang

“…However, its mechanism of action remains poorly understood.MethodsThe effects of αAsarone on autophagy were examined by WB, RT-qPCR, immunofluorescence colocalization, transmission electron microscope, and autophagic flux activity was measured by infecting HT22 cells with mRFP-GFP-LC3 adenovirus. …”
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To construct and validate a risk score model of angiogenesis-related genes to predict the prognosis of hepatocellular carcinoma by Duangui Gao, Yuan Lu, Tianpeng Jiang, Qinghong Duan, Zhi Huang

“…Finally, in the three pairs of HCC tissues and tissue adjacent to carcinoma by real-time fluorescent quantitative PCR (qRT_PCR) and western blotting (WB) to evaluate angiogenesis-related genes (ATP2A3 AEBP1 PNMA1, PLAT) expression level in HCC, and AEBP1 was knocked out in HCCLM3 cells, which is to study AEBP1 biological function in HCC. …”
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Fraxinellone-mediated targeting of cathepsin B leakage from lysosomes induces ferroptosis in fibroblasts to inhibit hypertrophic scar formation by Wei Xu, Hao Lv, Yaxin Xue, Xiaofeng Shi, Shaotian Fu, Xiaojun Li, Chuandong Wang, Danyang Zhao, Dong Han

“…Quantitative real-time PCR (qRT‒PCR), Western blot (WB), immunofluorescence staining and collagen gel contraction assays were performed to evaluate the collagen production and activation capacity of HSFs. …”
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TC2N maintains stem cell-like characteristics to accelerate lung carcinogenesis by blockade of dual specificity protein phosphatase 3 by Jing Gu, Yang-fan Lv, Ji-ying Xia, Fu-hai Bai, Ji Gong, Guang-qiang Pan, Bo Liu, Lu Huang, Qiao-nan Guo, Xiang-lin Hao

“…The protein levels of TC2N and stem cell markers in cell lines and tissue specimens were monitored by WB and immunohistochemistry. In vitro cell assays were performed to assess the effect of TC2N ectopic expression on the stem cell-like characteristics of lung cancer cells. …”
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Efficacy of Functional Endoscopic Sinus Surgery and Impact of Chronic Lung Disease on its Outcome in Patients of Chronic Rhinosinusitis by Minakshmi Karmakar, V D Prasanna Kumar Vasamsetty, Bonapart Chowdhury

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Prediction of Compressive Strength Behavior of Ground Bottom Ash Concrete by an Artificial Neural Network by Kraiwut Tuntisukrarom, Raungrut Cheerarot

“…Four input parameters, specifically, the water-to-binder ratio (WB), percentage replacement of GBA (PR), median particle size of GBA (PS), and age of concrete (AC), were considered for this prediction. …”
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Analysis of Top Quark Pair Production Signal from Neutral 2HDM Higgs Bosons at LHC by Majid Hashemi, Mahbobeh Jafarpour

“…The production mechanism is pp→H/A→tt¯ assuming a fully hadronic final state through t→Wb→jjb. In order to distinguish the signal from the main background which is the standard model tt¯, we benefit from the fact that the top quarks in signal events acquire large Lorentz boost due to the heavy neutral Higgs boson. …”
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Land Cover and Land Surface Temperature in the West Bank, Palestine by Ayah Helal, Zahraa Zawawi

“…The 10 major cities in the West Bank (WB), Palestine—Nablus, Ramallah and Al-Bireh, Jenin, Qalqilia, Salfit, Tubas, Jericho, Bethlehem, Tulkarem, and Hebron—are experiencing rapid urban transformation and changing land cover. …”
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Health Assessment of Large Two Dimensional Structures Using Limited Information: Recent Advances by Ajoy Kumar Das, Achintya Haldar, Subrata Chakraborty

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