Sox9-Increased miR-322-5p Facilitates BMP2-Induced Chondrogenic Differentiation by Targeting Smad7 in Mesenchymal Stem Cells by Yongsheng Zeng, Chengcheng Du, Pengcheng Xiao, Yiting Lei, Piao Zhao, Zhenglin Zhu, Shengqiang Gao, Bowen Chen, Shengwen Cheng, Wei Huang, Chen Zhao

“…The targeting relationship between miR-322-5p and Smad7 was confirmed by dual-luciferase reporter assays, qPCR, and western blotting (WB). The in vitro study indicated that overexpression of miR-322-5p significantly inhibited Smad7 expression, thus causing increased chondrogenic differentiation and decreased hypertrophic differentiation, while silencing of miR-322-5p led to the opposite results. …”
Get full text

Chemical reactivity of alliin and its molecular interactions with the M proteasepro of SARS-CoV-2 by López-Orozco Wendolyne, Mendoza-Huizar Humberto Luis, Álvarez-Romero Giaan Arturo, Torres-Valencia Jesús Martín, Sanchez-Zavala Maricruz

“…In the present work, a computational study of the chemical reactivity of alliin at the X/DGDZVP level of theory (where X is B3LYP, M06, M06L or wB97XD) was performed. The distribution of active sites on alliin was determined by evaluating the Fukui function. …”
Get full text

Preparation and Characterization of Ink from Hibiscus sabdariffa, Curcuma longa and Sorghum vulgare by Idara U. Ntuen, Aniekan Effiong Akpakpan, Godwin Enin, Iniobong Sylvester Enengedi, Emaime Jimmy Uwantta, Edet William Nsi, Itoro E. Udo, Edidiong Emmanuel Ikpe, Ukana Davies Akpabio

“…Drying times for the formulated inks varied from 3 –11 minutes, compared to 1-3 minutes for the control ink, with methanol-based formulated inks (MbFI) drying faster than water-based formulated ink (WbFI). The produced stamp pad demonstrated good ink adhesion on paper. …”
Get full text

脊髓小胶质细胞GLP-1受体/IL-10/β-内啡肽通路及其与独一味镇痛作用的关系 by 王永祥

“…本文简述我们实验室最近发现的脊髓小胶质细胞GLP-1受体/IL-10/β-内啡肽通路及其与一些中草药镇 痛作用之间的关系。GLP-1，艾塞那肽和WB4-24激动脊髓GLP-1受体，通过cAMP/PKA/p38β/CREB信号转导通路 合成/表达IL-10，后者通过自分泌方式活化IL-10受体/STAT3通路合成/表达β-内啡肽，从而在多种慢性疼痛模型 产生显著镇痛作用。…”
Get full text

Salubrinal Regulates the Apoptosis of Adrenocortical Carcinoma Cells via the PERK/eIF2α/ATF4 Signaling Pathway by Lili Wu, Chunfeng Liang, Xuemei Huang, Xiujun Deng, Jiming Jiang, Zuojie Luo

“…The expression of PERK/eIF2α/ATF4 signaling-pathway-related proteins and apoptosis-related proteins was detected by western blot (WB). Intracellular Ca2+ ion concentration was determined by a confocal laser scanning microscope. …”
Get full text

Efficacy of Inhaled Steroid Over Systemic Steroid in Stabilizing Acute Asthma in Emergency Room by B.V. Siva Vara Prasad Rao, Santhosh K B, Anil Sankol S M

Get full text

Dual chamber solar dryers: Maximizing thermal performance for diverse product drying by C. Naveen Prabhu, S. Dhanushkodi, K. Sudhakar

“…The hybrid drying mode achieved a target moisture content of 8% (w.b) within 9 hours with an average efficiency of 36%. …”
Get full text

Electrodeposited CdTe solar cells using low-purity Cd precursors & the effect of chemical purity by Ashfaque E. Alam, Hussain I. Salim, Ayotunde A. Ojo, Imyhamy M. Dharmadasa

Get full text

Zebularine Promotes Hepatic Differentiation of Rabbit Bone Marrow Mesenchymal Stem Cells by Interfering with p38 MAPK Signaling by Yong-Heng Luo, Juan Chen, En-Hua Xiao, Qiu-Yun Li, Yong-Mei Luo

“…BMMSCs were treated with zebularine at concentrations of 10, 20, 50, and 100 μM in the presence of hepatocyte growth factor; changes in the levels of hepatic-specific alpha-fetoprotein and albumin were detected and determined by RT-PCR, WB, and immunofluorescence staining. Expression of DNMT1 and phosphorylated p38 as well as urea production and ICG metabolism was also analyzed. …”
Get full text

African swine fever in the Primorsky Krai: disease situation and molecular and biological properties of the isolate recovered from a wild boar long bone by А. R. Shotin, A. S. Igolkin, Ali Mazloum, I. V. Shevchenko, N. S. Bardina, E. O. Morozova, А. А. Shevtsov

“…Analysis of biological properties of ASFV/Primorsky 19/WB-6723 isolate recovered from the long bone of a dead wild boar in the Primorsky Krai suggested that the isolate is highly virulent, able to cause peracute to subacute disease and up to 100% mortality among infected animals. …”
Get full text

LIFU Alleviates Neuropathic Pain by Improving the KCC2 Expression and Inhibiting the CaMKIV–KCC2 Pathway in the L4–L5 Section of the Spinal Cord by Ye-Hui Liao, Bin Wang, Mo-Xian Chen, Yao Liu, Li-Juan Ao

“…We used the 50% paw withdraw threshold (PWT50) to evaluate mechanical allodynia. Western blotting (WB) and immunofluorescence (IF) were used to calculate the expression of phosphorylated extracellular signal–regulated kinase 1/2 (p-ERK1/2), calcium/calmodulin-dependent protein kinase type IV (CaMKIV), phosphorylated cyclic adenosine monophosphate response element-binding protein (p-CREB), and KCC2 in the L4–L5 portion of the spinal cord after the last behavioral tests. …”
Get full text

<i>Aspergillus oryzae</i> Fermented <i>Plumula Nelumbinis</i> Against Atopic Dermatitis Through AKT/mTOR and Jun Pathways by Fengfeng Chen, Jing Liu, Xinwei Yu, Honglei Jia, Cheng Yang, Bingtian Zhao

“…<b>Methods:</b> The potential targets of FPN for AD were preliminarily screened using network pharmacology, and then PCR and WB were used to prove the therapeutic effect of FPN in AD. …”
Get full text

The Mutual Inhibition of FoxO1 and SREBP-1c Regulated the Progression of Hepatoblastoma by Regulating Fatty Acid Metabolism by Yu Hu, Hongyan Zai, Wei Jiang, Zhenglin Ou, Yuanbing Yao, Qin Zhu

“…FoxO1, SREBP-1c, FASN, ACLY, ACC, and MAGL expressions in tissue samples were detected by RT-qPCR and WB. IHC was utilized to measure FASN content. Overexpression and knockdown of FoxO1 and sSREBP-1c were performed on Huh-6 cells. …”
Get full text

Effect of intramuscular fat level on carcass composition, physicochemical characteristics, texture, and microstructure of breast muscle of broiler chickens by Dariusz Kokoszyński, Karol Włodarczyk, Joanna Żochowska-Kujawska, Marek Kotowicz, Marcin Wegner, Kamil Stęczny, Dorota Cygan-Szczegielniak

“…To date, there have been no studies determining the effect of IMF level on microstructure and texture traits of m. pectoralis major (except WB shear force) and electrical conductivity in Ross 308 broiler chickens. …”
Get full text

Cryoablation combined with dual immune checkpoint blockade enhances antitumor efficacy in hepatocellular carcinoma model mice by Jun Gu, Zepeng Yu, Xiangxiang Tang, Wenying Chen, Xuedong Deng, Xiaoli Zhu

“…The expression of apoptosis-related proteins was detected by Western blot (WB) assay. The percentages of immune cells and immunosuppressive cells were analyzed by flow cytometry. …”
Get full text

Rhopalurus junceus scorpion venom induces G2/M cell cycle arrest and apoptotic cell death in human non-small lung cancer cell lines by Alexis Díaz-García, Ángel Garrido, Jenny Laura Ruiz-Fuentes, Tamara Hermosilla, Diego Varela

“…The effects on cell cycle, apoptosis, and cell signaling-related proteins were determined by flow cytometry and WB. Protein fractions responsible for the observed effect were identified using HPLC. …”
Get full text

ＮＡＮＯＧ基因在侧群表型干细胞样肝癌细胞中的表达及意义

“…【方法】 采用流式细胞术检测和分选肝癌细胞Ｈｕｈ７和Ｈｅｐ３Ｂ中的ＳＰ细胞和非侧群（ＮＳＰ）细胞，细胞生长曲线法检测ＳＰ和ＮＳＰ细胞的增殖能力，Ｔｒａｎｓｗｅｌｌ侵袭实验检测ＳＰ和ＮＳＰ细胞的侵袭能力，ＭＴＴ法检测ＳＰ和ＮＳＰ细胞对５－氟尿嘧啶（５－ＦＵ）和阿霉素（ＤＯＸ）的药物敏感性；Ｒｅａｌ－ｔｉｍｅ ＰＣＲ和ＷＢ检测ＳＰ与ＮＳＰ细胞中ＮＡＮＯＧ基因的表达。【结果】肝癌细胞Ｈｕｈ７和Ｈｅｐ３Ｂ中分选的ＳＰ细胞比例分别为（５．３ ± ０．８）％和（１３．４９ ± １．０）％。…”
Get full text

上调ｍｉＲ－１００降低宫颈癌细胞侵袭和迁移及顺铂耐药性

“…ｔｒａｎｓｗｅｌｌ法观察细胞侵袭性，划痕实验观察迁移性；ＷＢ测定上皮间质变相关蛋白Ｅ－Ｃａｄｈｅｒｉｎ、Ｓｎａｉｌ、Ｖｉｍｉｎｔｉｎ及ＭＭＰ２、ｕＰＡ的表达；ＣＣＫ８法测定顺铂耐药性。 …”
Get full text

Non-Destructive Early Detection of Drosophila Suzukii Infestation in Sweet Cherries (c.v. <i>Sweet Heart</i>) Based on Innovative Management of Spectrophotometric Multilinear Corre... by Giuseppe Altieri, Mahdi Rashvand Avaei, Attilio Matera, Francesco Genovese, Vincenzo Verrastro, Naouel Admane, Orkhan Mammadov, Sabina Laveglia, Giovanni Carlo Di Renzo

Get full text

AIM2 promotes excitatory glutamate receptor expression by inhibiting STING and contributes to bone cancer pain in male mice by Linhan Wang, Xueqin Xu, Shanchun Su, Biyun Li, Kunyu Zhang, Xiuqin Yu, Yangqiao Xiao, Shuangshuang Lu, Zihao Lu, Yanqiong Wu, Changbin Ke

“…The protein expressions and localization were evaluated by qRT-PCR and WB or IF, respectively. The mechanical pain threshold was measured using the von Frey test. …”
Get full text