Intricate interplay of CRISPR-Cas systems, anti-CRISPR proteins, and antimicrobial resistance genes in a globally successful multi-drug resistant Klebsiella pneumoniae clone by Jianping Jiang, Astrid V. Cienfuegos-Galletd, Tengfei Long, Gisele Peirano, Tingyu Chu, Johann D. D. Pitout, Barry N. Kreiswirth, Liang Chen

“…The molecular epidemiology, population structure, and time phylogeny were investigated. The virulome, resistome, and mobilome were analyzed, and the recombination in the capsular region was studied. …”
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Interleukin IL1a and IL6 gene polymorphism: search for association with APOE genotype and clinical course of sporadic Alzheimer’s disease by G. Pšemeneckienė, K. Petrikonis, D. Rastenytė

“…Genotyping of IL1A -889C>T (rs1800587) and IL6 -174G>C (rs1800795, Intron type) was performed using real-time PCR (RT-PCR). Results. The distribution of IL1A -889C>T genotypes did not differ between APOE ε4 carriers (APOE4+: C/C – 52.9%, C/T – 41.2%, T/T – 5.9%) and APOE negative AD patients (APOE4-: C/C – 55.6%, C/T – 37.0%, T/T – 7.4%; p=0.887). …”
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Antibiotic knowledge and influencing factors among residents in China: a cross-sectional survey by Sixian DU, Yaqing LIU, Xiaoxu YIN, Haoran NIU, Feng JIANG, Liwen GONG

“…According to the multivariate logistic regression analysis, antibiotic knowledge scores were significantly influenced by the following factors (all P < 0.05): individuals with middle school education or below compared to those with a master′s degree or above (β = – 2.047, OR = 0.129), those with a medical education background compared to those without (β = – 1.430, OR = 0.239), full-time workers compared to other occupations (β = 0.387, OR = 1.472), healthcare workers compared to non-healthcare workers (β = – 0.674, OR = 0.510), individuals with chronic diseases compared to those without (β = 0.753, OR = 2.123), and those who self-rated their health as "very good" compared to "average" (β = 0.647, OR = 1.910). …”
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Web-based interventions for fear of cancer recurrence: A scoping review with a focus on suggestions for the development and evaluation of future interventions. by Solveiga Zibaite, Sheela Tripathee, Helen Moffat, Beatrix Elsberger, Sara Maclennan

“…Fourth, while web-based interventions do not involve the costs of transportation, traveling time, space, equipment, cleaning, and other expenses, further cost utility analyses should be performed. …”
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Impact of new coalbed methane wells on old well productivity and its controlling factors: a case study of Shizhuangnan block in Qinshui Basin by Xueting HAN, Shangzhi MENG, Guangjing LIU, Zhenyu REN, Shu TAO, Xinyang MEN, Ziyang WEI

“…While the production can be significantly restored by manual real-time unclogging, failure in unclogging results in a substantial decrease in gas production. …”
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Bioequivalence study of fluticasone propionate nebuliser suspensions in healthy Chinese subjects by Feng Cheng, Tao Shen, Fucheng Zhang, Chenghao Lei, Ye Zhu, GuoJun Luo, Dawei Xiao

“…The blood sample was collected at predetermined time points up to 48 h and the plasma concentration of fluticasone propionate was determined by HPLC-MS/MS in healthy subjects after inhalation of test or reference formulation. …”
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Effects of milk extracellular vesicles from Holstein Friesian and Brown Swiss heat-stressed dairy cows on bovine mammary epithelial cells by S. Castellani, L. Basiricò, A. Maggiolino, C. Lecchi, P. De Palo, U. Bernabucci

“…Cell viability and apoptosis assay, reactive oxygen species production, and mRNA expression of heat shock proteins and antioxidant genes by reverse transcription and real time PCR were determined. In vivo results showed an increase in rectal temperature and respiration rate, a reduction in milk yield both for H and B dairy cows, with a lowest decrease observed in B cows compared with H cows. …”
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Physical activity and content in a variety of physically active learning: an observational case study of real-world practices by Jan-Michael Johansen, Mathias Brekke Mandelid, Mathias Brekke Mandelid, Michael Reinboth, Geir Kåre Resaland, Solfrid Bratland-Sanda

“…All enacted PAL were passively observed by the same observer regarding primary bodily movement, duration, subject, location, collaborative or individual work, and task orientation.ResultsOn average, PAL consisted of 57.8% ± 15.7% sedentary time, 22.9% ± 7.5% light intensity PA, and 19.3% ± 10.8% moderate-to-vigorous intensity PA, displaying a large variety in different PAL segments. …”
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Human Umbilical Cord-Derived Mesenchymal Stem Cell Therapy Ameliorates Nonalcoholic Fatty Liver Disease in Obese Type 2 Diabetic Mice by Bing Li, Yu Cheng, Songyan Yu, Li Zang, Yaqi Yin, Jiejie Liu, Lin Zhang, Yiming Mu

“…One week after the sixth infusion, intraperitoneal glucose tolerance tests and insulin tolerance tests were performed and the blood and liver were harvested for biochemical and histopathological examinations. Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR), immunofluorescence staining, and western blot were performed to monitor the expression of the lipid metabolism- and regulatory pathway-related genes. …”
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Antibiotic Resistance and Molecular Epidemiological Characteristics of Streptococcus agalactiae Isolated from Pregnant Women in Guangzhou, South China by Zhaomin Cheng, Pinghua Qu, Peifeng Ke, Xiaohan Yang, Qiang Zhou, Kai Lan, Min He, Nannan Cao, Sheng Qin, Xianzhang Huang

“…Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) mass peak analysis was performed to discriminate the specific sequence types (STs). …”
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Altered lumbo-pelvic-hip complex muscle morphometry and contraction change in postpartum pelvic girdle pain and asymptomatic subjects: a cross-sectional study by Ziling Lin, Ziling Lin, Yanjun Hou, Xueling Chen, Yanping Liu, Yanping Liu, Xiangbin Wang, Xiangbin Wang

“…Therefore, this study aims to compare the differences in muscle thickness and contraction function of lumbo-pelvic-hip complex muscle between postpartum women with PGP and asymptomatic controls using ultrasound imaging.MethodsOne hundred and fifty postpartum women with PGP and fifty age-matched asymptomatic postpartum women were recruited in this study. Real-time musculoskeletal ultrasound was utilized to measure the resting muscle thickness of nine lumbo-pelvic-hip complex muscles, including the erector spinalis (ES), latissimus dorsi (LD), quadratus lumbalis (QL), gluteus maximus (GMax), gluteus medius (GMed), piriformis (PF), iliacus muscle (IM), rectus femoris (RF), and biceps femoris (BF). …”
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Correlation between liver fibrosis in non-alcoholic fatty liver disease and insulin resistance indicators: a cross-sectional study from NHANES 2017–2020 by Bo Yang, Mingsu Gong, Xiaojie Zhu, Yang Luo, Ruiqiu Li, Hai Meng, Yuhan Wang

“…These findings underscore the clinical value of incorporating IR indices into routine practice to identify high-risk patients, enabling timely interventions to prevent fibrosis progression and improve outcomes.…”
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Neuroprotective Effects of Eugenol Acetate Against Ischemic Stroke by Chen L, Zhang R, Xiao J, Liang Y, Lan Z, Fan Y, Yu X, Xia S, Yang H, Bao X, Meng H, Xu Y, Yu L, Zhu X

“…Liqiu Chen,1,2,&ast; Ran Zhang,1,2,&ast; Jing Xiao,1,2,&ast; Ying Liang,1,2 Zhen Lan,3 Yingao Fan,2 Xi Yu,2 Shengnan Xia,2– 5 Haiyan Yang,2– 5 Xinyu Bao,2– 5 Hailan Meng,1– 6 Yun Xu,1– 6 Linjie Yu,1– 6 Xiaolei Zhu1– 6 1Department of Neurology, Nanjing Drum Tower Hospital, Clinical College of Nanjing University of Chinese Medicine, Nanjing, 210008, People’s Republic of China; 2Department of Neurology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, People’s Republic of China; 3Department of Neurology, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing, 210008, People’s Republic of China; 4State Key Laboratory of Pharmaceutical Biotechnology and Institute of Translational Medicine for Brain Critical Diseases, Nanjing University, Nanjing, 210008, People’s Republic of China; 5Jiangsu Provincial Key Discipline of Neurology, Nanjing, 210008, People’s Republic of China; 6Nanjing Neurology Medical Center, Nanjing, 210008, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Linjie Yu; Xiaolei Zhu, Department of Neurology, Nanjing Drum Tower Hospital, Clinical College of Nanjing University of Chinese Medicine, Nanjing, 210008, People’s Republic of China, Email yulinjie126@126.com; zhuquelee@126.comObjective: To explore the neuroprotective effect of Eugenol Acetate (EA) on post-stroke neuroinflammation and investigate the underlying mechanisms.Methods: For in vitro experiments, primary microglia were pre-incubated with EA for 2 hours, followed by lipopolysaccharide (LPS) stimulation for 24 hours or Oxygen-Glucose Deprivation (OGD) treatment for 4 hours. Real-time quantitative PCR, enzyme-linked immunosorbent assay (ELISA) and Western blot were performed to examine the expression levels of inflammatory cytokines in primary microglia. …”
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MRI to digital medicine diagnosis: integrating deep learning into clinical decision-making for lumbar degenerative diseases by Baoyi Ke, Wenyu Ma, Junbo Xuan, Junbo Xuan, Yinghao Liang, Liguang Zhou, Wenyong Jiang, Jing Lin, Guixiang Li

“…Additionally, the testing time of the deep learning program was significantly shorter than that of manual identification, and the differences were statistically significant (P &lt; 0.05).ConclusionsDeep learning technology based on the PP-YOLOv2 algorithm can be used to identify normal intervertebral discs, lumbar disc herniation, and lumbar spondylolisthesis from lumbar MRI images. …”
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Clinically relevant orthotopic pancreatic cancer models for adoptive T cell transfer therapy by Gregory B. Lesinski, Chrystal M. Paulos, Maggie Phillips, Jacklyn Hammons, Isaac Karpovsky, Zaid Mahdi, Natalie K. Horvat, Megan M. Wyatt, Margaret A. Hall, Cameron J. Herting, Richard A. Moffitt

“…Interestingly, we found that chimeric antigen receptor (CAR) T cells redirected to recognize mesothelin on these tumors that signal via CD3ζ and 41BB (Meso-41BBζ-CAR T cells) infiltrated the tumors of mice bearing stroma-devoid KPC-Luc orthotopic tumors, but not MT-5 tumors.Conclusions Our data establish for the first time a reproducible and realistic clinical system useful for modeling stroma-rich and stroma-devoid PDAC tumors. …”
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Evaluation and Management of Patients with Hematoma After Gynecologic and Obstetric Surgery by Bekir Kahveci, Mehmet Obut, Serhat Ege, Mete Sucu, Nurullah Peker, Osman Uzundere, Gaye Kahveci, Mehmet Sukru Budak

“…In addition, 4 patients (13.3%) had relaparotomy, 5 patients (16.7%) underwent percutaneous radiological drainage and 8 (26.7%) received blood transfusion. The mean time of resorption of the hematoma was 4.6 ± 2.0 days. …”
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Clinical outcomes of early remdesivir administration in hospitalized patients at high risk for severe COVID-19 during the Omicron wave by Yeon Ju La, Won Sup Oh, Changhyup Kim, Myoung-nam Lim, Yong Duk Jeon

“…We included patients aged ≥ 18 years who had symptom onset within 7 days and had at least one risk factor for disease progression at the time of diagnosis. We compared the clinical outcomes between the early remdesivir administration group and the group not administered early remdesivir. …”
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Deciphering Immunometabolic Landscape in Rheumatoid Arthritis: Integrative Multiomics, Explainable Machine Learning and Experimental Validation by Dong Q, Wu J, Zhang H, Chen X, Xu X, Chen J, Shi C, Luo L, Zhang D

“…Machine learning models identified hub genes, which were validated via quantitative real-time PCR (qRT-PCR). Then, potential small-molecule drugs were screened using the Connectivity Map (CMAP) and molecular docking. …”
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Physical modelling of damage mechanism of slopes containing near horizontal weak layers under rainfall conditions by Zhigang TAO, Chuang XU, Songyuan LIU, Yong LI, Kexin XIU

“…From the results of displacement monitoring, it can be seen that the slope cracks can be up to 35 mm wide, indicating that the slope slippage damage. ② From the numerical simulation analysis, it can be seen that the slope deformation increases significantly under rainfall conditions, and the maximum deformation can be up to 40 cm without anchoring measures. At the same time, the closer to the coal seam position, the larger the area of the plastic zone of slope shear damage, and the landslide body appears to have a large area of shear damage after sliding along the weak layer of the rock body, which verifies the deformation form of slope shear-slip damage. ③ From the comparative analysis of the NPR anchor cable reinforcement scheme and the PR anchor cable reinforcement scheme, it can be seen that the supporting effect of the NPR anchor cable is much better than that of the PR anchor cable, the maximum displacement of the slope under the NPR anchor cable support is reduced by 17.6 cm, while the maximum displacement of the slope under the PR anchor cable support is reduced by only 10.3 cm. …”
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Circular RNA RHOT1 Regulates miR-142-5p/CCND1 to Participate in Chondrocyte Autophagy and Proliferation in Osteoarthritis by Gu Man, Haiwen Yang, Kai Shen, Donghong Zhang, Julin Zhang, Hao Wu, Hongyu Zhang, Jinhuan Wang

“…The function of circRHOT1 in the regulation of OA was assessed by CCK-8, colony formation, flow cytometry analysis, quantitative real-time PCR, Western blot analysis, and luciferase reporter gene assay in chondrocytes. …”
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