Investigation of natural infection of BALB C mice by Bartonella henselae by Luciene Silva dos Santos, Sayros Akyro Soares Martins, Francine Ramos Scheffer, Alexandre Seiji Maekawa, Rafaela de Paula Silva, Gabriel Rabelo de Araújo, Paulo Eduardo Neves Ferreira Velho, Marina Rovani Drummond

“…DNA was extracted using a commercial kit and tested via nested PCR for the ftsZ gene, as well as conventional PCR and qualitative real-time PCR using Sybr® Green for the citrate synthase gene (gltA), all specific reactions for B. henselae. …”
Get full text

Acute effect of a Mediterranean-style dietary pattern (MDP) on mood, anxiety and cognition in UK adults with mild to moderate anxiety and depression: the MediMood randomised contro... by Emma Stevenson, Amy Jennings, Anne Marie Minihane, Rachel Gillings, Alpar Lazar, M Hornberger, Saber Sami, Latife Esgunoglu, Marrium Liaquat, Adrian Leddy, Jon Brooks, William Penny

“…A linear mixed model and/or paired analysis will be used to compare the effects of treatments over time.Ethics and dissemination The study has received a favourable ethics opinion from the National Health Service London Queen Square Research Ethics Committee (22/LO/0796). …”
Get full text

Performance improvements and increased ruminal microbial interactions in Angus heifers via supplementation with native rumen bacteria during high-grain challenge by Fan Yang, Madison T. Henniger, Andrew S. Izzo, Emily A. Melchior, Brooke A. Clemmons, Mary A. Oliver, James R. Gaffney, Cameron Martino, Taylor B. Ault-Seay, Miranda L. Striluk, Jordan J. Embree, Juan F. Cordero-Llarena, Pierre-Yves Mulon, David E. Anderson, Mallory M. Embree, Phillip R. Myer

“…Additionally, administration of NRM to animals on a high-grain diet, improved ruminal microbiome stability (P < 0.001), potentially encouraging the conversion of rumen lactate to propionate over time via the succinate pathway and alleviating metabolic stress.…”
Get full text

Optimization of bioactive compounds and sensory quality in thermosonicated black carrot juice: A study using response surface methodology, gradient boosting, and fuzzy logic by Seydi Yıkmış, Melikenur Türkol, Ishak Pacal, Aylin Duman Altan, Nazlı Tokatlı, Gholamreza Abdi, Nazan Tokatlı Demirok, Rana Muhammad Aadil

“…The study examined the effects of temperature, processing time, and ultrasonic amplitude on total carotenoid content (TCC), total anthocyanin content (TAC), ferric reducing antioxidant power (FRAP), and total phenolic content. …”
Get full text

Blood-based biomarkers suggest prolonged axonal Injury following pediatric mild traumatic brain injury by Andrew R. Mayer, Tracey V. Wick, Jessica R. McQuaid, Masen L. Boucher, Andrew B. Dodd, Cidney R. Robertson-Benta, Harm J. van der Horn, Erik B. Erhardt, Robert E. Sapien, Rawan Tarawneh, Rebekah Mannix

“…Abstract Pediatric mild traumatic brain injury (pmTBI) affects millions of youth annually but underlying pathophysiology and time for physiological recovery remains unknown. …”
Get full text

Overexpression of HIF-2α, TWIST, and CXCR4 Is Associated with Lymph Node Metastasis in Papillary Thyroid Carcinoma by Ni Wang, Hao-Jun Luo, Guo-Bing Yin, Chao-Ran Dong, Man Xu, George G. Chen, Zhi-Min Liu

“…Additionally, HIF-2α, TWIST, and CXCR4 mRNA expression were assessed in 30 PTCs, 10 nodular hyperplasia, and 10 normal thyroid tissue specimens using real-time RT-PCR. TWIST and CXCR4 mRNA expression levels were up-regulated in PTCs, and high mRNA expression of TWIST and CXCR4 was significantly correlated with LNM (P=0.005 and P=0.010, resp.). …”
Get full text

Clinical and molecular analyses of isolated central congenital hypothyroidism based on a survey conducted in Japan by Nao Shibata, Chikahiko Numakura, Takashi Hamajima, Kenichi Miyako, Ikuma Fujiwara, Jun Mori, Akihiko Saitoh, Keisuke Nagasaki

“…The neonatal period is the optimal time for the diagnosis of central CH, particularly IGSF1 abnormalities, and the introduction of T4 screening should be considered in the future, taking cost-effectiveness into consideration.…”
Get full text

Acute Pain Burden and Opioid Dose Requirements after Cesarean Delivery in Parturients with Preexisting Chronic Back Pain and Migraine by Ryu Komatsu, Michael G. Nash, Kenneth C. Ruth, William Harbour, Taylor M. Ziga, Shane Mandalia, Emily M. Dinges, Davin Singh, Hani El-Omrani, Joseph Reno, Brendan Carvalho, Laurent A. Bollag

“…The effects of the three chronic pain conditions on time-weighted average (TWA) pain score (primary outcome) and opioid dose requirements in morphine milligram equivalents (MME) during postoperative 48 hours were compared. …”
Get full text

The effect of resveratrol on the cryopreservation of Mongolian horse semen by M. Du, M. Du, M. Du, Y. Liu, Y. Liu, Y. Liu, L. Zhang, L. Zhang, L. Zhang, X. Li, X. Li, X. Li, N. Wang, N. Wang, N. Wang, Q. He, Q. He, Q. He, J. Cao, J. Cao, J. Cao, B. Zhao, B. Zhao, B. Zhao, Y. Shi, Y. Shi, Y. Shi, B. Li, B. Li, B. Li, G. Bou, G. Bou, G. Bou, M. Dugarjaviin, M. Dugarjaviin, M. Dugarjaviin

“…These findings were further validated through real-time fluorescent quantitative PCR and protein immunoblotting, suggesting that RSV has potential as an effective antioxidant for improving the cryopreservation of Mongolian horse semen.…”
Get full text

Landscape connectivity alters the evolution of density-dependent dispersal during pushed range expansions by Dahirel, Maxime, Bertin, Aline, Calcagno, Vincent, Duraj, Camille, Fellous, Simon, Groussier, Géraldine, Lombaert, Eric, Mailleret, Ludovic, Marchand, Anaël, Vercken, Elodie

“…While theory suggests that evolution during range expansions could lead pushed expansions to become pulled with time, empirical comparisons of phenotypic divergence in pushed vs. pulled contexts are lacking. …”
Get full text

Lipid Coating Modulates Effects of Nanoceria on Oxidative Metabolism in Human Embryonic Lung Fibroblasts: A Case of Cardiolipin by Elena V. Proskurnina, Madina M. Sozarukova, Elizaveta S. Ershova, Ekaterina A. Savinova, Larisa V. Kameneva, Natalia N. Veiko, Maria A. Teplonogova, Vladimir P. Saprykin, Vladimir K. Ivanov, Svetlana V. Kostyuk

“…We used an MTT assay, fluorescence microscopy, real-time reverse transcription polymerase chain reaction, and flow cytometry. …”
Get full text

Electrochemically-driven solid oxide tubular membrane reactor for efficient separation of oxygen and argon by Yuanhui Tang, Yutao Hu, Sisi Wen, Song Lei, Yakai Lin, Li Ding, Haihui Wang

“…Inspired by the principle that O2 can permeate through the electrolyte as oxygen ions (O2-) in a solid oxide electrolysis cell, for the first time, this study designed and developed an electrochemically-driven tubular inorganic membrane reactor to separate O2/Ar mixtures, achieving high-purity Ar (≥99.99 %). …”
Get full text

HuoXueTongFu Formula Alleviates Intraperitoneal Adhesion by Regulating Macrophage Polarization and the SOCS/JAK2/STAT/PPAR-γ Signalling Pathway by Min Zhao, Yao-Yao Bian, Li-Li Yang, Yan-Qi Chen, Ya-Jie Wang, Yan-Ting Ma, Yu-Qiong Pei, Wen-Lin Li, Li Zeng

“…Histopathology, flow cytometry, ELISA, real-time PCR, and Western blotting were used to further detect the related protein, M1/M2 polarization tendency, and PPAR-γ nuclear translocation. …”
Get full text

Polydatin enhances oxaliplatin-induced cell death by activating NOX5-ROS-mediated DNA damage and ER stress in colon cancer cells by Qi Zhao, Qi Zhao, Qi Zhao, Yan Zhang, Yan Zhang, Jieyu Liu, Peipei Chen, Annabeth Onga, Namki Cho, Ri Cui, Chenguo Zheng

“…The relative gene and protein expressions were analyzed by quantitative real time-PCR (qRT-PCR) and Western blot assays. Molecular docking analysis predicted the interaction between PD and potential targets.ResultsWe found that PD exerted anti-CRC activity by promoting Nicotinamide Adenine Dinucleotide Phosphate (NADPH) oxidase 5 (NOX5)-mediated ROS production, activating the endoplasmic reticulum (ER) stress, and inducing DNA damage. …”
Get full text

Assessing the Accuracy of Dermoscopy for Scabies Diagnosis in Dark African Skin by Nkechi Anne Enechukwu, Ikenna Akuakolam, Iloduba N Aghanya, Chetanna C Anaje, Ogochukwu I Ezejiofor, Enzo Errichetti

“…While definitive diagnosis involves identifying adult mites, eggs, or scybala from the microscopy of scrapings obtained from representative sites; it is invasive, time-consuming and not feasible in very young or non-cooperating patients. …”
Get full text

Bioactive potential and storage behavior of low molecular mass peptides in Pilsner and IPA style craft beers by Roberta Nogueira Pereira da Silva, Angelica Priscila Parussolo Tonin, Gabriela Soares Macello Ramos, Juliana Furtado Dias, Eduardo Cesar Meurer, Maria Gabriela Bello Koblitz

“…Pilsner beers, typically clear due to added chill-proofing proteases, showed reduced peptide intensity over time, whereas IPA, which often remains hazy and lacks such enzymes, exhibited increased peptide levels. …”
Get full text

Evaluation of the effectiveness of the Iran-package of essential non-communicable disease (IraPEN) program in reducing cardiovascular disease risk in pilot areas by Somayeh Derakhshan, Davood Khalili, Alireza Mahdavi, Seyed-Saeed Hashemi-Nazari, Amir Kavousi, Fatemeh Hadavandsiri, Afshin Ostovar, Koorosh Etemad

“…The trend analysis of risk factors over time confirmed these findings. Conclusion The program was modestly effective in reducing CVD risk in the pilot areas. …”
Get full text

Exploring the relationship between eco-anxiety and suicide risk in adolescents with mental health disorders: insights from a cross-sectional observational study by Apolline Lerolle, Jean-Arthur Micoulaud-Franchi, Jean-Arthur Micoulaud-Franchi, Pierre Fourneret, Pierre Fourneret, Alexandre Heeren, Alexandre Heeren, Christophe Gauld, Christophe Gauld

“…Although, the univariate model indicated a significant association between the CAS and the C-SSRS (β = 2.58; p = 0.049), the cognitive/emotional difficulties and functional impairment dimensions of eco-anxiety, considering different confounding factors, did not show statistical associations with the severity of suicide risk (respectively, p = 0.81 and p = 0.76).DiscussionIn an expansive literature, these results show for the first time that eco-anxiety may not be the priority of adolescents seen by adolescent psychiatrists. …”
Get full text

The Impact of Astaxanthin Supplementation on the Lipid Profile by Filip Arczewski, Michalina Wójcikiewicz, Witold Czyż, Julia Kacperczyk, Karol Dziedzic, Marta Chuncia, Maciej Wojszczyk, Damian Zys, Piotr Pasek, Julia Ryniecka, Wiktor Czaja, Michał Krzciuk

“…Evidence suggests that AST effectively prolongs the oxidation lag time of LDL, thereby mitigating the risk of atherosclerosis and cardiovascular diseases through its antioxidative mechanisms. …”
Get full text

Machine learning-based modelling and analysis of carbonation depth of recycled aggregate concrete by Xuyong Chen, Xuan Liu, Shukai Cheng, Xiaoya Bian, Xixuan Bai, Xin Zheng, Xiong Xu, Zhifeng Xu

“…The top five factors impacting RAC carbonation depth are exposure time, water-to-binder ratio, CO2 concentration, coarse aggregate density, and cement content. …”
Get full text