Agathisflavone Inhibits Viability and Modulates the Expression of miR-125b, miR-155, IL-6, and Arginase in Glioblastoma Cells and Microglia/Macrophage Activation by Karina Costa da Silva, Irlã Santos Lima, Cleonice Creusa dos Santos, Carolina Kymie Vasques Nonaka, Bruno Solano de Freitas Souza, Jorge Mauricio David, Henning Ulrich, Ravena Pereira do Nascimento, Maria de Fátima Dias Costa, Balbino Lino dos Santos, Silvia Lima Costa

“…Agathisflavone (5–30 μM) induced a dose- and time-dependent reduction in the viability of both human GL-15 and rat C6 cells, as determined by the MTT test, and reduced cell migration, as determined by cell scratch assay. …”
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Production and characterization of nervous necrosis virus vaccine antigens in wild type and CRISPR/Cas9 genome edited Nicotiana benthamiana and edible crop lettuce Lactuca sativa by Iva Andrasevic, Hang Su, Espen Rimstad, Jihong Liu Clarke

“…In the present study, we report: (1) selection and design of recombinant plasmids encoding the capsid proteins (CPs) of two genotypes of NNV, red-spotted grouper nervous necrosis virus (RGNNV) and striped jack nervous necrosis virus (SJNNV); (2) design and optimization of plasmids for transient expression of NNV vaccine antigens in wild type Nicotiana benthamiana, CRISPR/Cas9 genome edited Nicotiana benthamiana and Lactuca sativa; (3) test of different Agrobacterium strains (LBA4404 and AGL1) for effective production of NNVCPs; (4) the expression patterns of NNVCPs over time post infiltration for different plants and cultivars; (5) successful production of NNV antigens in N. benthamiana lines and lettuce cultivars, indicating the potential of the plants as antigen producers in the development of a plant-based vaccine against VNN.…”
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Evaluating the effectiveness of a mental health literacy module - A randomized controlled trial among school teachers by Vidya Prabhu, Lena Ashok, Veena Ganesh Kamath, Amitha Puranik, Sebastian Padickaparambil, Nikitha Sibil Rebello, Praveen Arhanthabailu, Varalakshmi Chandrasekaran

“…Repeated measures ANOVA adjusted for cluster effect was used to assess the change in knowledge, management, attitudes and beliefs over time in the intervention arm. Results: Significant improvement was observed in the intervention arm as compared to the control arm across the domains of knowledge and attitudes and beliefs with an increase in mean knowledge score of 3.34 (95 % CI: 2.14 to 4.55 p < 0.001) and an increase in mean attitudes and beliefs score of 0.95 (95 % CI: 0.42 to 1.49 p < 0.001). …”
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Effect of a Nutritional Education Intervention on the Reduction of Added Sugar Consumption in Schoolchildren in Southeastern Mexico: Community Study by Carmen Morales-Ruán, Teresa Shamah-Levy, Danae Gabriela Valenzuela-Bravo, Rebeca Uribe-Carvajal, Corin Hernández-Palafox, María Concepción Medina-Zacarías, Ignacio Méndez Gómez-Humarán

“…By contrast, the intervention effect, represented by the interaction between treatment and time, showed a 10% reduction in the final stage in the intervention group (<i>p</i> < 0.001). …”
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Application of three-dimensional printing in the planning and execution of aortic aneurysm repair by Harshank Patel, Paul Choi, Jennifer C. Ku, Rosemary Vergara, Rafael Malgor, Dhiren Patel, Yong Li

“…This review examines the application of 3D printing and AI in complex aortic aneurysm repair, highlighting current clinical trends.MethodsAn exhaustive literature review was performed using keywords such as “3D printing,” “Artificial intelligence,” “Thoracoabdominal aneurysm,” “Abdominal aortic aneurysm,” “Aortic arch aneurysm,” “Endovascular repair,” and “Open repair” in PubMed and Google Scholar indexes up to June 2022.ResultsThis analysis included seven studies: four focused on 3D-printed phantoms for endovascular repair of various aortic pathologies (aortic arch, thoracoabdominal aorta, juxtarenal and pararenal aorta), one on open thoracoabdominal aneurysm repair using 3D-printed models for graft construction, and two on the use of convolutional neural networks, an AI-based technology, for the pre-processing of aortic computed tomography angiography images.ConclusionThe application of 3D printing and AI-based image pre-processing in the planning of complex aortic aneurysms offers several benefits, including enhanced patient and trainee education, more accurate fenestration placement, reduced surgical time and complications, and decreased surgeon stress.…”
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PER3 suppresses tumor metastasis of oral squamous cell carcinoma by promoting HIF-1α degradation by Yaoxu Li, Bing Li, Kai Yang, Lihua Zhu, Hong Tang, Yinpei Huang, Jinhai Deng, Jun Duan

“…PER3 expression was decreased in OSCC, and PER3 expression was significantly negatively correlated with T stage, N stage, clinical classification, and survival time. PER3 overexpression inhibited, while PER3 knockdown prompted EMT and metastasis of OSCC cells. …”
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Performances of asphalt mastic incorporating with a home-made sustained-releasing salt storage additive by Wei Du, Qing-liang Wang, Yong-xu Li, Xiao-ning Wang, Xiao-cun Liu, Ya-fei Wang, Yan-min Wang, Jin Li

“…However, in our study the DSR experimental design was constrained to temperature scanning, neglecting frequency and time domain scanning, which precluded a comprehensive assessment of mid-temperature fatigue performance. …”
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Simultaneous Determination of 5 Components in the Leaves of Dimocarpus longan by Quantitative Analysis of Multicomponents by Single Marker (QAMS) Based on UPLC and HPLC by Jiani Mai, Jie Liang, XianFu Liu, LiuPing Tan, Hui Xu, YaoHua Li, YuShan Zhou, ChuanChuan Yang, ChenXi Xin

“…Relative correction factors (RCFs, ƒs/i) of the other 4 components were calculated by two correction methods (multipoint correction and slope correction) to effectuate QAMS. At the same time, the difference between the results measured by the QAMS and external standard methods was compared to verify the accuracy of QAMS. …”
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SIV infection induces alterations in gene expression and loss of interneurons in Rhesus Macaque frontal cortex during early systemic infection by Richard C. Crist, Samar N. Chehimi, Saurabh S. Divakaran, Michael J. Montague, Sébastien Tremblay, Noah Snyder-Mackler, Martin O. Bohlen, Kenneth L. Chiou, Trish M. Zintel, Cayo Biobank Research Unit, Michael L. Platt, Halvor Juul, Guido Silvestri, Matthew R. Hayes, Dennis L. Kolson, Benjamin C. Reiner

“…Differential expression identified alterations in cellular subtype gene expression that persisted over the 20-day time course and short-lived differences only detected at 10-days post-SIV infection. …”
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Near-Infrared Spectroscopy as a Process Analytical Technology Tool for Monitoring the Steaming Process of Gastrodiae rhizoma with Multiparameters and Chemometrics by Yamin Zuo, Jing Yang, Chen Li, Xuehua Deng, Shengsheng Zhang, Qing Wu

“…This method holds a great promise to replace current subjective color judgment and time-consuming HPLC or UV/Vis methods and is suitable for rapid online monitoring and quality control in the TCM industrial steaming process.…”
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<i>Rosa damascena</i> Mill. Essential Oil: Analysis of In Vitro and In Vivo Genotoxic and Cytotoxic Potentials by Employing Three Cytogenetic Endpoints by Tsvetelina Gerasimova, Svetla Gateva, Gabriele Jovtchev, Tsveta Angelova, Margarita Topashka-Ancheva, Ana Dobreva, Milka Mileva

“…The rose essential oil showed varying concentration- and time-dependent cytotoxic and genotoxic effects depending on the test system used, and it was established that the oil showed moderate cytotoxicity in lymphocyte cultures and non-high cytotoxicity in ICR mice but none in barley. …”
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Review of environmental monitoring in freshwater lakes using geospatial techniques (remote sensing and GIS) by Kamilia Kamaruzzaman, Siti Aekbal Salleh, Faezah Pardi, Muhamad Fuad Abdullah, Vladimir Foronda, Emerson L. Bergonio, Rahmawaty Rahmawaty

“…The review covers the time from 2018 to 2023. The reviews follow established methods and procedures for systematically identifying, evaluating, and testing the study literature. …”
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Ultrasound-guided percutaneous thermal ablation of parotid tumors: experience from two-centers by Min Zhuang, Yucheng Lin, Songsong Wu, Man Lu, Zirui Jiang, Ting Wei, Lu Wang, Shishi Wang, Jie Zou, Yi He

“…Mean CS also changed from 3.39 ± 0.66 to 1.75 ± 0.93 (p < 0.001) by the end of follow-up time. Subgroup analysis showed that tumors with smaller initial maximum diameter had a faster CS reduction rate than those with larger initial diameter. …”
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Naringenin inhibits lipid accumulation by activating the AMPK pathway in vivo and vitro by Xiaoyu Cai, Songxue Wang, Huali Wang, Suwen Liu, Guishan Liu, Huibin Chen, Ji Kang, Hao Wang

“…The molecular mechanism of naringenin in the treatment of obesity were predicted by using network pharmacology. Real-time PCR analysis results showed that naringenin regulated the expression of lipid metabolism genes. …”
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miRNA Expression Profile and Effect of Wenxin Granule in Rats with Ligation-Induced Myocardial Infarction by Aiming Wu, Lixia Lou, Jianying Zhai, Dongmei Zhang, Limin Chai, Bo Nie, Haiyan Zhu, Yonghong Gao, Hongcai Shang, Mingjing Zhao

“…The expressions of miR-1, miR-133, Cx43, and Cx45 were detected by quantitative real-time PCR. It was found that 35 differentially expressed miRNAs and 23 predicted pathways, including miR-1, miR-133, and gap junction pathway, are involved in the pathogenesis of MI. …”
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Sensory alterations and immunological changes during the chronification of postsurgical pain: a study protocol for a prospective observational cohort study by Leo Koenderman, Niels Eijkelkamp, Frank J P M Huygen, Roel J H Custers, Marjelle E C van Driel, Nadine van Veenendaal, Lisette Margaretha Vernooij, L Timmerman, Diyar Delawi, M Rijsdijk

“…Study participants complete questionnaires, undergo quantitative sensory testing (QST) and provide blood samples to assess the immunological response at various time points: before surgery, 2 weeks and 3 months after surgery. …”
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MetaboLINK is a novel algorithm for unveiling cell-specific metabolic pathways in longitudinal datasets by Jared Lichtarge, Gerarda Cappuccio, Gerarda Cappuccio, Soumya Pati, Soumya Pati, Alfred Kwabena Dei-Ampeh, Alfred Kwabena Dei-Ampeh, Senghong Sing, Senghong Sing, Senghong Sing, LiHua Ma, Zhandong Liu, Zhandong Liu, Mirjana Maletic-Savatic, Mirjana Maletic-Savatic, Mirjana Maletic-Savatic

“…This method is designed to reduce the dimensionality of large datasets while preserving significant variance. For the first time, we applied the PCA-GLASSO algorithm (i.e., MetaboLINK) to metabolomics data derived from Nuclear Magnetic Resonance (NMR) spectroscopy performed on neural cells at various developmental stages, from human embryonic stem cells to neurons.ResultsThe MetaboLINK analysis of longitudinal metabolomics data has revealed distinct pathways related to amino acids, lipids, and energy metabolism, uniquely associated with specific cell progenies. …”
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Predictors of HBsAg seroclearance in HBeAg-negative chronic hepatitis B patients treated with nucleotide analogs plus polyethylene glycol interferon by Yan Peng, Mingzhe Ma, Ting Liu, Wenmin He, Shutao Lin, Wa Zhong, Xiaohui Min

“…Moreover, for the first time, we found ALT change values from baseline to week 24 (dALT2) could act as a novel independent clinical efficacy predictors in the forementioned population.…”
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Characterization of the Collagen Extraction Manufacturing Process Using Markov Chains and Artificial Neural Networks by Rosa Trasvina-Osorio, Sergio Alonso-Romero, Juan de Anda-Suarez, Valentin Calzada-Ledesma, Javier Yanez-Mendiola, Luis Fernando Villanueva-Jimenez, Erick Rojas-Mancera

“…The product of this research is a four-dimensional predictive model that estimates the yield with decreasing material, energy resources, and the time required for the collagen extraction process from the skin waste of tilapia. …”
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Vitamin D Status in Central Europe by Pawel Pludowski, William B. Grant, Harjit Pal Bhattoa, Milan Bayer, Vladyslav Povoroznyuk, Ema Rudenka, Heorhi Ramanau, Szabolcs Varbiro, Alena Rudenka, Elzbieta Karczmarewicz, Roman Lorenc, Justyna Czech-Kowalska, Jerzy Konstantynowicz

“…The decrease in 25(OH)D with respect to age was attributed to decreased time spent in the sun and decreased vitamin D production efficiency. …”
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