Optimization of Enzymatic Hydrolysis Process, Structural Characterization and Antioxidant Activity Analysis of Gastrodia elata Polysaccharide by Yunfei HU, De ZHOU, Qinglan ZENG, Jinsong HE, Ming ZHAO, Qingyan TANG

“…Results revealed that the optimal enzymolysis process was as follows: enzyme dosage, 460 U/g, enzymolysis time, 100 min, enzymolysis temperature, 60 ℃, enzymolysis pH, 5.4. …”
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Data from Emergency Medical Service Activities: A Novel Approach to Monitoring COVID-19 and Other Infectious Diseases by Daniele del Re, Luigi Palla, Paolo Meridiani, Livia Soffi, Michele Tancredi Loiudice, Martina Antinozzi, Maria Sofia Cattaruzza

“…Results show that EMS data mirrored the time series of cases or deaths in Lombardy, with good agreement also being found with seasonal flu outbreaks. …”
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Halothermophilic Cas9 and dCas9 Interaction with Variety sgRNA In-silico by Kenny Lischer, Kholisoh Hayati, Muhammad Haykal Fabian, Fina Amreta Laksmi, Yudhi Nugraha, Hamdan Dwi Rizqi, Riri Fauziyya, Sarmoko, Mohd Shukuri Mohamad Ali

“…Molecular Docking has advantages such as cost-effectiveness and time efficiency in designing the optimal sgRNA length. …”
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Information and mathematical modelling in construction in the development of building products for fire detection in a neighboring building by A. A. Lazarev, S. V. Fedosov, V. G. Kotlov, D. E. Tsvetkov, A. Yu. Komlev

“…The main approaches to solving these problems are related to the theory of heat transfer. At the same time, there is practically no development of algorithms for the use of building products to detect a fire in a neighboring building.Materials and methods. …”
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Correlation of artificial neural network and multi-attribute transformation: a test case to predict the effective porosity of Cretaceous sandstones of the Sembar Formation, southea... by Furqan Aftab, Furqan Aftab, Furqan Aftab, Furqan Aftab, Mohammad Zafar, Muhammad Iqbal Hajana, John H. Shaw

“…We suggest that it is theoretically possible for the ANN to make predictions about any attribute of the reservoir via bridging target logs and seismic data within a short computation time.…”
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Bioactive Steroids with Structural Diversity from the South China Sea Soft Coral <i>Lobophytum</i> sp. and Sponge <i>Xestospongia</i> sp. by Lin-Mao Ke, Zi-Ru Zhang, Song-Wei Li, Yan-Bo Zeng, Ming-Zhi Su, Yue-Wei Guo

“…The structures of these compounds were determined by extensive spectroscopic analysis, the time-dependent density functional theory–electronic circular dichroism (TDDFT-ECD) calculation method, and comparison with the spectral data previously reported in the literature. …”
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Expression of Sclerostin in Osteoporotic Fracture Patients Is Associated with DNA Methylation in the CpG Island of the SOST Gene by Yanming Cao, Bin Wang, Ding Wang, Dongxiang Zhan, Caiyuan Mai, Peng Wang, Qiushi Wei, Yamei Liu, Haibin Wang, Wei He, Liangliang Xu

“…After extracting total RNA and protein, we checked the relative expression levels of SOST by quantitative real-time PCR and western blot. Also, immunohistochemical staining was performed to observe the expression of SOST protein in the bone samples. …”
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Optimal Pore Size of Honeycomb Polylactic Acid Films for In Vitro Cartilage Formation by Synovial Mesenchymal Stem Cells by Misaki Yagi, Mitsuru Mizuno, Ryota Fujisawa, Hisako Katano, Kentaro Endo, Nobutake Ozeki, Yuriko Sakamaki, Hideyuki Koga, Ichiro Sekiya

“…One day after plating, the medium was switched to chondrogenic induction medium, and the films were time-lapse imaged and observed histologically. Results. …”
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Cold case report: Geoforensic brownfield site search for murder victim based on prison informant lead by Jamie K. Pringle, Andrew Binley, Kristopher D. Wisniewski, Ben Davenward, Vivienne G. Heaton, Georgina E. Handley

“…The case presented suggests these geoforensic approaches provide assurances to rule out forensic search locations, saving time and costs in such cold case investigations.…”
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Effects of Physical Exercise Breaks on Executive Function in a Simulated Classroom Setting: Uncovering a Window into the Brain by Qian Yu, Zhihao Zhang, Sebastian Ludyga, Kirk I. Erickson, Boris Cheval, Meijun Hou, Dominika M. Pindus, Charles H. Hillman, Arthur F. Kramer, Ryan S. Falck, Teresa Liu‐Ambrose, Jin Kuang, Sean P. Mullen, Keita Kamijo, Toru Ishihara, David A. Raichlen, Matthew Heath, David Moreau, André O. Werneck, Fabian Herold, Liye Zou

“…Linear mixed models identified significant positive effects of exercise conditions on behavioral reaction time (RT), error rate, and inverse efficiency score (β = −2.62, −0.19, −3.04: ps < 0.05). …”
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Sorption isotherms and kinetics of Crystal Violet dye uptake from aqueous solution by using polyaniline nanocomposite as adsorbent by Piyush Gupta, Amrita Kaushik, Garima Nagpal, Markus Diantoro, Firdaus Mohamad Hamzah, Faisal Islam Chowdhury, Pramod K. Singh

“…Under optimal conditions (pH 9, 0.5 g of adsorbent in 50 ml of solution, and a 60-minute contact time), the ZnFe₂O₄-PANi nanocomposite exhibited endothermic and spontaneous adsorption characteristics. …”
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Effect of Glucosamine and Ascorbic Acid Addition on Beef Burger Textural and Sensory Attributes by Philip Soladoye, Yuliya Hrynets, Mirko Betti, Zeb Pietrasik

“…Except for L * (lightness) value, significant interaction ( p <0.01) between storage time and added ingredient was observed for all colour parameters ( a* ; redness, b* ; yellowness, chroma, and hue angle) in beef burger. …”
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Over-the-Scope Clip-Associated Endoscopic Muscular Dissection for Seven Cases of Small Gastric Submucosal Tumor: A Video-Based Case Series by Xin Li, Rongfen Wei, Jianfu Qin, Fei Qin, Peng Peng, Mengbin Qin, Shiquan Liu, Jiean Huang

“…OTSC-associated endoscopic muscular dissection as a novel endoscopic interventional therapy should be a convenient, safe, and effective therapy for small GSMT. The short-time outcome is excellent, whereas long-term effect is unclear, and the further follow-up is needed to schedule.…”
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Leveraging airborne imaging spectroscopy and multispectral satellite imagery to map glacial sediment plumes in Kachemak Bay, Alaska by L. Hartl, C. Schmitt, M. Stuefer, J. Jenckes, B. Page, C. Crawford, G. Schmidt, R. Yang, R. Hock

“…This informs the subsequent analysis of a homogenized, Rayleigh corrected time series of Landsat and Sentinel-2 images and seasonal patterns of turbidity.New Hydrological Insights for the RegionOur results provide the most comprehensive data set on water surface turbidity in Kachemak Bay to date and improve understanding of spatial and seasonal variability of glacial turbidity in a data sparse region. …”
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Diagnostics and treatment of peptic ulcer: clinical guidelines of the Russian gastroenterological Association by V. T. Ivashkin, A. A. Sheptulin, I. V. Mayev, Ye. K. Baranskaya, A. S. Trukhmanov, T. L. Lapina, S. G. Burkov, A. V. Kalinin, A. V. Tkachev

“…PUD diagnosis includes obligatory testing for H. pylori infection and carrying out eradication in the case of positive takes. At the present time the first line of antihelicobacter therapy includes standard triple clarithromycin and amoxicillin-based therapy, as well as quadrotherapy with bismuth drugs. …”
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A Landscape-Clustering Zoning Strategy to Map Multi-Crops in Fragmented Cropland Regions Using Sentinel-2 and Sentinel-1 Imagery with Feature Selection by Guanru Fang, Chen Wang, Taifeng Dong, Ziming Wang, Cheng Cai, Jiaqi Chen, Mengyu Liu, Huanxue Zhang

“…Crop mapping using remote sensing is a reliable and efficient approach to obtaining timely and accurate crop information. Previous studies predominantly focused on large-scale regions characterized by simple cropping structures. …”
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NRBF2 plays a crucial role in the acquisition process of learning and memory, independent of the Vps34 complex by Songfen Wu, Songfen Wu, Songfen Wu, Haicai Zhuang, Haicai Zhuang, Haicai Zhuang, Xidan Zhou, Kuan Li, Kuan Li, Kuan Li

“…IntroductionNRBF2, a component of autophagy-associated PIK3C3/VPS34-containing phosphatidylinositol 3-kinase complex, plays a crucial role in learning and memory processes, yet its specific impact on memory and the underlying molecular mechanisms remains unclear.MethodsHere, we utilized NRBF2 knockout mice to examine its influence on the time course of fear memory. Employing quantitative PCR, Western blot analysis, behavioral tests, and electrophysiology, we investigated the mechanisms through which NRBF2 affects memory processing.ResultsWe observed an increase in Nrbf2 mRNA levels at 6 and 12 h, and protein levels at 6 h post fear conditioning. …”
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Prevalence and investigation of Cytomegalovirus (HCMV) in blood donors from the main blood establishment in Rio de Janeiro/Brazil by Agildo da Silva Oliveira, Jéssica Gonçalves Pereira, Gabrielle Tantos Nunes, Ivanildo Pedro de Sousa Junior, Dmitry José de Santana Sarmento, Josiane Iole França Lopes, Luiz Amorim Filho, Vanessa Salete de Paula

“…Materials and methods: Here, we investigated the serological and molecular prevalence of HCMV among 980 blood donors from the main blood bank in Rio de Janeiro, Brazil, using chemiluminescence and real-time PCR (TaqMan). The data underwent univariate, bivariate, and multivariate statistical analyses using the SPSS program, version 20.0. …”
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Nursing experience of non-invasive ventilation in prone position for a patient with acute respiratory distress syndrome combined with COVID-19 (1例新型冠状病毒肺炎合并急性呼吸窘迫综合征患者俯卧位无创通气护理体会)... by WANG Jijun (王吉军), LIU Jiaojiao (刘佼佼), YANG Ting (杨婷)

“…In the early stage of COVID-19 complicated with ARDS, non-invasive ventilation in prone position can not only effectively reduce the tube intubation and improve hypoxemia, but also reduce the labor cost and level of difficulty in nursing, reduce the workload and exposure time of medical staff, and reduce the risk of infection among medical staff. …”
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A Sensitive, Specific and Fast Electrochemical‐based Nanobiosensor Diagnostic for Xanthomonas albilineans, the Cause of Sugarcane Leaf Scald Disease by Moutoshi Chakraborty, Shamsul Arafin Bhuiyan, Simon Strachan, Muhammad J.A. Shiddiky, Nam‐Trung Nguyen, Narshone Soda, Rebecca Ford

“…The molecular methods of detection require expensive equipment, labor‐intensive, and time‐consuming. This study proposes a novel electrochemical (EC)‐approach, that is relatively easy to use and less expensive to detect Xalb DNA in LS‐infected sugarcane leaves, meristematic tissue, and xylem sap samples. …”
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