Evaluation of circulating tumor DNA as a prognostic and predictive biomarker in BRAF V600E mutated colorectal cancer—results from the FIRE‐4.5 study by Susanne Klein‐Scory, Alexander Baraniskin, Wolff Schmiegel, Thomas Mika, Roland Schroers, Swantje Held, Kathrin Heinrich, David Tougeron, Dominik P. Modest, Ingo Schwaner, Jan Eucker, Rudolf Pihusch, Martina Stauch, Florian Kaiser, Christoph Kahl, Meinolf Karthaus, Christian Müller, Christof Burkart, Sebastian Stintzing, Volker Heinemann

“…This study was accompanied by a prospective translational project analyzing cell‐free circulating tumor DNA (ctDNA) in plasma to test whether ctDNA analysis may help to guide clinical treatment decision making. …”
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Perspectives and experiences regarding pre-exposure prophylaxis (PrEP) in a community sample of Veterans with unhealthy alcohol use: overall and across sexual orientation and gende... by Olivia V. Fletcher, Kristine Beaver, Elizabeth J. Austin, Jenna van Draanen, E. Jennifer Edelman, Emily C. Williams

“…Methods Using data collected from a national online survey of Veterans recruited via Facebook ads, community organizations, and listservs, we assessed variation in four PrEP outcomes (knowledge, experience, willingness, and conversations with doctors) across patterns of unhealthy alcohol use among all respondents reporting any lifetime drinking (n = 1,041) and then within sexual orientation and gender identity groups using Chi-square or Fisher’s exact tests. Results Among 1,041 eligible Veterans, 440 (42%) screened positive for unhealthy alcohol use. …”
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Intranasal delivery of hMSC-derived supernatant for treatment of ischemic stroke by inhibiting the pro-inflammatory polarization of neutrophils by Yixiang Jiang, Ning Wang, Jingyi Liu, Haoran Ren, Wenkang Jiang, Yanting Lei, Xidan Fu, Miao Hao, Xiujuan Lang, Yumei Liu, Xijun Liu, Rui Li, Hulun Li

“…The neuroprotective effects of hMSC-L and hMSC-M were assessed with TTC staining, behavioral tests and pathological staining. Flow cytometry and qPCR evaluated the expression of immune cells and cytokines in the CNS and peripheral immune organs. …”
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Serum ALT activity and its isoenzymes as potential biomarkers for diagnosis of Sarcopenia in older adults: a retrospective, cross-sectional study by Jiangping Zeng, Nannan Li, Jiaying Ge, Huihui Ma, Siqi Sun, Yujie Jing, Chunhua Qian, Ran Cui, Shen Qu, Hui Sheng

“…Hand grip strength (HGS) was measured with a digital dynamometer, and physical performance was assessed through the 6-meter gait speed and the five-times sit-to-stand test (FTSST). Binary logistic regression analysis was used to evaluate associations between ALT-related indicators (ALT activity and ALT1/ALT2 ratio) and sarcopenia. …”
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The transcription factor combination MEF2 and KLF7 promotes axonal sprouting in the injured spinal cord with functional improvement and regeneration-associated gene expression by Callan L. Attwell, Inés Maldonado-Lasunción, Ruben Eggers, Bastiaan A. Bijleveld, Ward M. Ellenbroek, Natascha Siersema, Lotte Razenberg, Dédé Lamme, Nitish D. Fagoe, Ronald E. van Kesteren, August B. Smit, Joost Verhaagen, Matthew R. J. Mason

“…Next, adeno-associated viral vectors were used to express these TF combinations in vivo in L4/L5 dorsal root ganglia to test whether they would promote regeneration in a spinal cord injury model (dorsal column lesion) in female rats. …”
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Thiol-Disulfide Homeostasis in Noise-Induced Hearing Loss in Rats by Enes Aydın, Serpil Mungan Durankaya, Osman Yilmaz, Günay Kirkim, Safiye Aktaş, Salim Neşelioğlu, Özcan Erel, Yüksel Olgun, Abdullah Dalgıç

“…The auditory brainstem response (ABR) test was performed in all groups on day 1 after noise exposure and was repeated in groups 1, 2, and 3 on day 5. …”
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Fibrinogen-albumin ratio predicts treatment response in phospholipase A2 receptor-associated membranous nephropathy with nephrotic syndrome by Suyan Duan, Si Chen, Chen Chen, Fang Lu, Ying Pan, Yifei Lu, Qing Li, Simeng Liu, Bo Zhang, Huijuan Mao, Changying Xing, Yanggang Yuan

“…The Kaplan-Meier survival analysis demonstrated that the high-FAR group (>0.233) had a significantly lower probability to achieve CR or PR compared to the low-FAR group (≤0.233) (Log Rank test, p = 0.021). Higher levels of FAR were identified as an independent risk factor for NR, and the high-FAR group was associated with a 2.27 times higher likelihood of NR than the low-FAR group (HR 2.27, 95% CI 1.01, 5.13, p = 0.048). …”
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Preparation of Tragopogon graminifolius-loaded electrospun nanofibers and evaluating its wound healing activity in a rat model of skin scar by Leila Almasi, Elham Arkan, Mohammad Hosein Farzaei, Amin Iranpanah, Cyrus Jalili, Fatemeh Abbaszadeh, Faranak Aghaz, Sajad Fakhri, Javier Echeverría

“…[Asteraceae] (TG), also called “Sheng” in traditional Iranian medicine, is one of the most efficacious plants for treating various skin injuries due to its several pharmacological and biological effects like anti-inflammatory and antioxidant properties.PurposeIn the present study, our objective was to assess the wound-healing activity of PVA/PEO/CS nanofibers containing TG in a rat model of excision wound repair.MethodsSynthesized nanofibers from PVA, PEO, and CS were done by the electrospinning method and confirmed by scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FT-IR). The release tests of nanofibers were assessed through the UV-visible method at different time intervals, which were conducted for about 60 h. …”
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A novel α-conotoxin [D1G, ΔQ14] LvIC decreased mouse locomotor activity by Wen Wang, Wen Wang, Meiting Wang, Huanbai Wang, Weifeng Xu, Conggang Wang, Jie Pei, Xiaodan Li, Dongting Zhangsun, Dongting Zhangsun

“…Following this procedure, behavioral tests were conducted to assess changes in the mice’s behavior. …”
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Genome analysis of colistin-resistant Salmonella isolates from human sources in Guizhou of southwestern China, 2019–2023 by Jingtong Wu, Yongxian Wen, Lv You, Xiaoyu Wei, Xiaoyu Wei, Junhua Wang, Ge Zhu, Shijun Li

“…Therefore, it is necessary to monitor CL-R Salmonella in Guizhou and systematically elucidate their characteristics-related resistance, virulence, and molecular epidemiology to develop effective public health strategies against resistant pathogens.MethodsThe CL-R Salmonella isolates were identified from 933 Salmonella isolates by antimicrobial resistance testing. To further evaluate the molecular epidemiology, the CL-R Salmonella isolates underwent whole-genome sequencing (WGS) analysis followed by bioinformatic analysis.ResultsA total of 43 CL-R isolates (4.6%) were identified from 933 Salmonella isolates, of which 39 isolates being MDR (resistance to three or more classes of antimicrobials). …”
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Evaluation of microRNAs as liquid biopsy markers in adrenocortical tumors by Chrysoula Mytareli, Vassiliki Kalotychou, Alexandros Lafioniatis, Gregory Kaltsas, George N. Zografos, Athina Markou, Labrini Papanastasiou, Athanasios Fountas, Dimitra Argyro Vasilliadi, Evanthia Kassi, Evanthia Kassi, Marina Mantzourani, Anna Angelousi

“…Additionally, circulating miR-22-3p levels exhibited a significant correlation with traditional diagnostic tests for hypercortisolism.ConclusionThis study supports the potential of a liquid biopsy approach as an innovative method for diagnosing and monitoring patients with ATs, offering a complementary tool to existing diagnostic methods.…”
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Assessing the feasibility of measuring residents’ quality of life in English care homes and the construct validity and internal consistency of measures completed by staff proxy: a... by Claire Goodman, Julienne Meyer, Karen Spilsbury, Barbara Hanratty, Rachael Carroll, Adam L Gordon, Gizdem Akdur, Anne Killett, Jennifer Burton, Ann-Marie Towers, Stacey Rand, Nick Smith, Sinead Palmer, Stephen Allan, Lucy Anne Webster

“…Objectives To assess the feasibility of capturing older care home residents’ quality of life (QoL) in digital social care records and the construct validity (hypothesis testing) and internal consistency (Cronbach’s alpha) of four QoL measures.Design Cross-sectional data collected in wave 1 of the DACHA (Developing resources And minimum dataset for Care Homes’ Adoption) study, a mixed-methods pilot of a prototype minimum dataset (MDS).Setting Care homes (with or without nursing) registered to provide care for older adults (>65 years) and/or those living with dementia. …”
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Interpreting IGF-1 in children treated with recombinant growth hormone: challenges during early puberty by Helena-Jamin Ly, Helena-Jamin Ly, Carina Ankarberg-Lindgren, Hans Fors, Hans Fors, Staffan Nilsson, Jovanna Dahlgren, Jovanna Dahlgren

“…Levels of estradiol in girls and testosterone in boys were analyzed from previously collected frozen samples.ResultsNine of 58 (15.5%) estradiol samples in girls with Tanner breast stage 1 had pubertal levels of estradiol ≥25 pmol/L. For boys with testes size <4 mL, 24 out of the 153 (15.7%) testosterone samples were above the pubertal cut-off, ≥0.47 nmol/L. …”
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Investigating root and canal morphology of anterior and premolar teeth using CBCT with a novel coding classification system in Saudi subpopulation by Mohammed Mustafa, Mohmed Isaqali Karobari, Ali Abdullah Alwan Al-Maqtari, Abdulaziz Abdulwahed, Ahmed A. Almokhatieb, Laila S. Almufleh, Qamar Hashem, Abdullah Alsakaker, Mohammad Khursheed Alam, Hany Mohamed Aly Ahmed

“…Demographic data including gender (male and female) and age (divided into 6 groups—10–20, > 20–30, > 30–40, > 40–50, > 50–60, > 60–70) were recorded. The Chi-square test was used for statistical analysis, and the significance level was set at 0.05 (P = 0.05). …”
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Effect of a prepartum and postpartum, complex interdisciplinary lifestyle and psychosocial intervention on metabolic and mental health outcomes in women with gestational diabetes m... by Umberto Simeoni, Antje Horsch, Arnaud Chiolero, Yvan Vial, Nicole Sekarski, Jardena J Puder, Pascal Bovet, Yvan Mivelaz, Adina Mihaela Epure, Bobby Stuijfzand, Leah Gilbert, Stefano Lanzi, Justine Gross, Didier Hans, Stefano Di Bernardo, Elena Gonzalez-rodriguez, Alain Lacroix, Jean-Benoît Rossel, Dan Yedu Quansah, Amar Arhab, Sandrine Estoppey Younes, Seyda Mayerat

“…Objective To test the effect of a complex, interdisciplinary, lifestyle and psychosocial intervention on metabolic and mental health outcomes in women with gestational diabetes mellitus during pregnancy and in the post partum.Design Single centred, single blinded, randomised, controlled trial (the MySweetheart trial).Setting Lausanne University Hospital, Switzerland, from 2 September 2016 to 25 October 2021.Participants 211 women aged at least 18 years with a diagnosis of gestational diabetes mellitus at 24-32 gestational weeks were randomly assigned (1:1) to the intervention (n=105) or to usual care (n=106).Interventions In addition to a comparator based on active guidelines for prepartum and postpartum usual care, the intervention consisted of four individual lifestyle visits during pregnancy and four interdisciplinary visits in the postpartum group, a peer support group workshop in pregnancy and post partum, and a bimonthly lifestyle coach support through telemedicine. …”
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Exploring the clinical value of procalcitonin, c-reactive protein, white blood cell count, and neutrophil-to-lymphocyte ratio in the early diagnosis of bloodstream infections in ch... by Yadong Li, Mingjie Li, Chenye Lin, Wentao Tang, Qiuyu Tang, Feng Cheng

“…Combined use of serum PCT, CRP, WBC, and NLR testing can improve the diagnostic sensitivity of pediatric BSI, reducing the risk of missed diagnoses, thereby enhancing the early diagnostic value of pediatric BSI.…”
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Differences in the Cardiovascular Risk Assessment in Cardiology Outpatients in Mali: Comparison between Framingham Body Mass Index-Based Tool and Low-Information World Health Organ... by Bâ Hamidou Oumar, Sangaré Ibrahima, Camara Youssouf, Sidibé Noumou, Coulibaly Souleymane, Cissoko Yacouba, Sidibé Samba, Konaté Massama, Maiga Asmaou Kéita, Doumbia Coumba Thiam, Touré Mamadou, Diakité Mamadou, Menta Ichaka, Diall Ilo Bella

“…After sample description, the risk score was assessed using bmi-Frm and li-WHO prediction tools. Finally, a kappa test was performed to check for the interreliability of both methods. …”
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Mamá Empoderada: study protocol for a pilot trial of a novel parenting and mental health prevention intervention for migrant mothers with young children at the Mexico-US border by Gudelia Rangel, Shira M Goldenberg, Eileen Pitpitan, Kaylee Ramage, Ietza Bojorquez-Chapela, Cecilia Martinez-Torteya, Katherine Rosenblum, Chelsie Hernandez, Monica Alvarez

“…The aim of this study is to pilot-test a novel parenting and mental health prevention intervention for migrant mothers with young children (MMC) in Tijuana, Mexico, including (a) assessing acceptability; (b) estimating effect sizes on symptoms of depression, anxiety, and parenting stress; (c) identifying which theory-based mechanisms of action predict changes in outcomes; and (d) identifying factors associated with differential intervention response.Methods and analysis ‘Mamá Empoderada’ (Mom Power) is a theory-based, trauma-informed group intervention to promote mental health and responsive parenting among mothers with young children (0–5 years). …”
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RETRACTED: Postoperative Pulmonary Complications in Patients With Transcatheter Tricuspid Valve Implantation—Implications for Physiotherapists by Peng-Ming Yu, Peng-Ming Yu, Yu-Qiang Wang, Ze-Ruxing Luo, Raymond C. C. Tsang, Oystein Tronstad, Oystein Tronstad, Jun Shi, Ying-Qiang Guo, Alice Y. M. Jones

“…Spirometry lung function, maximal inspiratory pressure (MIP), and 6-min walk test distance (6MWD) were recorded. Prior to surgery, patients were stratified into high or low pulmonary risk groups based on published predefined criteria. …”
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Nomogram to predict 3 month prognosis of acute ischemic stroke among young adults by Qian He, Qian He, Miaoran Wang, Haoyue Zhu, Ying Xiao, Rui Wen, Xiaoqing Liu, Yangdi Shi, Linzhi Zhang, Bing Xu

“…We used the area under the receiver-operating characteristic curve (ROC) to evaluate the discriminative performance and used the calibration curve with Hosmer-Lemeshow goodness of fit test to assess the calibration performance of the risk prediction model. …”
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