Large-scale synthesis of polydimethylsiloxane as vitreous replacement applications by Ajrina Nur Shabrina, Diba Grace Auliya, Risdiana, Lusi Safriani

“…This study reported that the result of the synthesis of PDMS on a scale three and five times larger than the lab-scale using a ring-opening polymerization method with octamethylcyclotetrasiloxane (D4) as a monomer and hexamethyldisiloxane as a chain terminator by increasing the volume of raw materials and reactors. …”
Get full text

Genetic Transformability of Selected Kenyan Groundnut (Arachis hypogaea L.) Genotypes with IPT Gene Using Cotyledonary Node Explants by Purity G. Limbua, Mathew P. Ngugi, Richard O. Oduor

“…The cotyledonary nodes of six Kenyan adapted groundnuts genotypes (ICGV 12991, CG 7, Red Valencia, ICGV 90704, Chalimbana, and JL 24) were transformed using Agrobacterium tumefaciens strain EHA 101 carrying PNOV-IPT binary vector containing an IPT gene, which was driven by SARK promoter and terminated by TNOS terminator. The vector also contained the phosphomannose isomerase (PMI) gene for the selection of transformed tissues. …”
Get full text

ParSite is a multicolor DNA labeling system that allows for simultaneous imaging of triple genomic loci in living cells. by Xiaohui He, Yadong Sun, Hanhui Ma

“…The ParSite permits tracking promoter and terminator dynamics of the APP gene, which spans 290 kilobases in length. …”
Get full text

Overexpression of the Synthetic Chimeric Native-T-phylloplanin-GFP Genes Optimized for Monocot and Dicot Plants Renders Enhanced Resistance to Blue Mold Disease in Tobacco (N. taba... by Dipak K. Sahoo, Sumita Raha, James T. Hall, Indu B. Maiti

“…The synthetic chimeric gene (native-phylloplanin-GFP) was placed between the modified Mirabilis mosaic virus full-length transcript promoter with duplicated enhancer domains and the terminator sequence from the rbcSE9 gene. The chimeric gene, expressed in transgenic tobacco, was stably inherited in successive plant generations as shown by molecular characterization, GFP quantification, and confocal fluorescent microscopy. …”
Get full text

Improved constructs for bait RNA display in a bacterial three-hybrid assay by Linh D. Nguyen, Hannah LeBlanc, Katherine E. Berry

“…In this work, we introduce new bait RNA constructs to (1) insulate the folding of individual components of the hybrid RNA with GC clamps and (2) express bait RNAs that do not encode their own intrinsic terminator. We find that short GC clamps (5 or 7 bp long) are more effective than a longer 13 bp GC clamp in the B3H assay. …”
Get full text

Evaluation of various RNA-seq approaches for identification of gene outrons in the flatworm Opisthorchis felineus by N. I. Ershov, D. E. Maslov, N. P. Bondar

“…One is based on the enzymatic elimination of overrepresented cDNAs, the other utilizes exonucleolytic degradation of uncapped RNA by Terminator enzyme. By using the outron-targeting methods, we were not able to obtain the enrichment of RNA preparations by processed outrons, which is most likely indicative of a rapid turnover of these trans-splicing intermediate products. …”
Get full text

Identification of <i>Tinomiscium petiolare</i> from Vietnam using the DNA barcode by B. B. Thinh, R. V. Doudkin, L. D. Chac, H. V. Chinh, Q. V. Hoi, N. T. Lien

“…The purified PCR products were sequenced on the ABI 3500 Genetic Analyzer system, using BigDye®Terminator v3.1 Cycle Sequencing Kit. These genetic sequences were analyzed and compared, and a phylogenetic tree was constructed using BioEdit, BLAST, and MEGA 6 programs.Results and conclusion. …”
Get full text

The Effects of SLS on Structural and Complex Permittivity of SLS-HDPE Composites by Abubakar Dantani Meli, Zulkifly Abbas, Mohd Hafiz Mohd Zaid, Nor Azowa Ibrahim

“…The SLS-HDPE composites can be applied in the area of waveguide as a microwave waveguide terminations and dummy loads.…”
Get full text

Research progress in the role of β-arrestin 1 in the regulation of inflammatory response by YI Wenjing, FAN Yixuan, QIU Jiatai, FU Xiaoyan, LIU Meifang

“…However, an uncontrolled inflammatory response that occurs when the body is subjected to more severe stimuli may often cause serious harm to the body. The termination of the ideal inflammatory response occurs after the elimination of the inflammatory stimulus. …”
Get full text

Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album by A. B. Beklemishev, M. B. Pykhtina, Ya. M. Kulikov, T. N. Goryachkovskaya, D. V. Bochkov, S. V. Sergeeva, A. R. Vasileva, V. P. Romanov, D. S. Novikova, S. E. Peltek

“…The proteinase K gene was inserted into pPICZα-A in such a way that – at a subsequent stage of transfection into yeast cells – it was efficiently expressed under the control of the promoter and terminator of the AOX1 gene, and the product of the exogenous gene contained the signal peptide of the Saccharomyces cerevisiae a-factor to ensure the protein’s secretion into the culture medium. …”
Get full text

Assessing the Impact of Aldose Reductase (ALR2) Regulatory Gene Polymorphism in Diabetic Retinopathy Patients by Hadiza Rabe Musa, Amina Rabe Musa, Yahuza Gimba Muhammed, Usman Salisu Batagarawa, Aminu Ibrahim

“…Subsequently, DNA sequencing was performed using the ABI Prism BigDyeTM Terminator Cycle Sequencing Ready Reaction Kit on the ABI PrismTM 3730/3730XL DNA Sequencer. …”
Get full text

Disproportionate Distribution of HBV Genotypes A and D and the Recombinant Genotype D/E in the High and Low HBV Endemic Regions of Uganda: A Wake-Up Call for Regional Specific HBV... by Hussein Mukasa Kafeero, Dorothy Ndagire, Ponsiano Ocama, Charles Drago Kato, Eddie Wampande, Henry Kajumbula, David Kateete, Abdul Walusansa, Ali Kudamba, Kigozi Edgar, Fred Ashaba Katabazi, Maria Magdalene Namaganda, Jamilu E. Ssenku, Hakim Sendagire

“…Automated DNA sequencing was performed using BigDye Terminator v3.1 Cycle Sequencing Kit on 3130 Genetic Analyzer (Applied Biosystems, USA). …”
Get full text

Molecular Epidemiology, Drug-Resistant Variants, and Therapeutic Implications of Hepatitis B Virus and Hepatitis D Virus Prevalence in Nigeria: A National Study by Oludare ‘Sunbo Adewuyi, Muhammad Shakir Balogun, Hirono Otomaru, Alash’le Abimiku, Anthony Agbakizu Ahumibe, Elsie Ilori, Que Anh Luong, Nwando Mba, James Christopher Avong, John Olaide, Oyeladun Okunromade, Adama Ahmad, Afolabi Akinpelu, Chinwe Lucia Ochu, Babatunde Olajumoke, Haruka Abe, Chikwe Ihekweazu, Adetifa Ifedayo, Michiko Toizumi, Hiroyuki Moriuchi, Katsunori Yanagihara, Jide Idris, Lay-Myint Yoshida

“…We assessed 732 HBV DNA-extracted samples with detectable viral loads (VLs) for (sub-)genotypes and variants by whole-genome pre-amplification, nested PCR of the <i>s</i>-and <i>pol</i>-gene, and BigDye Terminator sequencing. We conducted HDV serology. In total, 19 out of the 36 + 1 states in Nigeria had a high prevalence of HBV (≥8%), with the highest prevalence (10.4%) in the north-central geopolitical zone. …”
Get full text

Optimization of Port Emergency Materials Dispatch with Time Windows Constraints under Uncertainties by Bowei Xu, Yuqing Wang, Junjun Li

“…For example, at the beginning of 2020, the sudden outbreak of COVID-19 seriously affected terminal production and increased the short-term pressure of handling at container terminals. …”
Get full text

Sequence Control Mechanism for the Handover of Distribution Networks Under Disaster Recovery by Cheng Long, Jian Zhang, Hua Zhang, Xing Liu, Yiwen Gao, Xueneng Su, Shilong Li

“…In the distribution network under the background of disaster, the distribution terminal embodies the characteristics of disorder. …”
Get full text

Energy consumption analysis for WLAN discovery schemes in converged networks by JIANG Jun-feng, CAO Zhi-gang

“…It is also shown that the scheme that keeps the terminal periodic scanning in the cell with WLAN and that keeps the terminal periodic scanning in all zones consume comparable energy.…”
Get full text

Novel Insights into Surface Energies and Enhanced Gas-Sensing Capabilities of ZnGa₂O₄(111) via Ab Initio Studies by Cheng-Lung Yu, Yan-Cheng Lin, Sheng-Yuan Jhang, Jine-Du Fu, Yi-Chen Chen, Po-Liang Liu

“…It explores the interactions of gas molecules such as NO, NO₂, and CH₃COCH₃ with Ga-terminated, O-terminated, and Ga-Zn-O-terminated surfaces. …”
Get full text

NR-U and Wi-Fi spectrum sharing for quality guaranteeing of power services by Junpeng LIU, Weiwei XIA, Han LIU, Chenglin XIU, Feng YAN, Lianfeng SHEN

“…To alleviate the shortage of 5G licensed spectrum resources, using unlicensed spectrum has become an important solution.With the large-scale access of power terminals, NR-U and Wi-Fi spectrum sharing for power services quality guaranteeing has become an important research hotspot.Firstly, an NR-U uplink transmission mechanism was proposed, which ensured the average throughput of Wi-Fi users and realized the data uplink transmission of power service terminals.In addition, a resource optimization algorithm for joint transmission time and subcarrier allocation (TTSA) was proposed to ensure the quality of service (QoS) of various types of power services and maximize the total throughput of terminals.The optimization problem was decoupled, and proximal policy optimization (PPO) was used to allocate subcarriers to terminals.The simulation results show that compared with the existing algorithms, the proposed resource optimization algorithm for TTSA has superior performance in guaranteeing the service quality of power services and maximizing the total terminals throughput.…”
Get full text

Dynamic optimization of a two-stage fractional system in microbial batch process by Xiaopeng Yi, Huey Tyng Cheong, Zhaohua Gong, Chongyang Liu, Kok Lay Teo

“…The objective function was the productivity of 1,3-propanediol at the terminal time, while the decision variables were the initial concentrations of biomass and glycerol, and the terminal time of the batch process. …”
Get full text