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281
Single‐ended 2 ch. × 3.4 Gbit/s dual‐mode near‐ground transmitter IO driver in 45 nm CMOS process
Published 2017-03-01Subjects: Get full text
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282
Accuracy of Established Prognostic Staging Systems for Cardiac Transthyretin Amyloidosis in the Tafamidis Era
Published 2025-02-01Subjects: Get full text
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283
DÂHİLÎ KORUNMA ALTERNATİFİ YAKLAŞIMI VE ULUSLARARASI KORUMA STATÜSÜ
Published 2024-07-01Subjects: Get full text
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284
The Reporter System for GPCR Assay with the Fission Yeast Schizosaccharomyces pombe
Published 2012-01-01“…Subsequently, LPI terminator was replaced with the corresponding downstream regions. …”
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285
Sežerou nás stroje?
Published 2025-01-01“…Nemusí jít jen o téma přežití lidstva jako ve filmu Terminátor. Již teď je zřejmé, že mnohá odvětví i profese projdou výraznou proměnou. …”
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286
Harnessing the potential of chloroplast-derived expression elements for enhanced production of cellulases in Escherichia coli
Published 2025-01-01“…A high-level of expression of β-glucosidase and endoglucanase in E. coli was recorded from the chloroplast promoter and terminator. Furthermore, the activity assays confirmed that the recombinant enzymes maintained their activity at elevated temperatures. …”
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287
Large-scale synthesis of polydimethylsiloxane as vitreous replacement applications
Published 2025-12-01“…This study reported that the result of the synthesis of PDMS on a scale three and five times larger than the lab-scale using a ring-opening polymerization method with octamethylcyclotetrasiloxane (D4) as a monomer and hexamethyldisiloxane as a chain terminator by increasing the volume of raw materials and reactors. …”
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288
Genetic Transformability of Selected Kenyan Groundnut (Arachis hypogaea L.) Genotypes with IPT Gene Using Cotyledonary Node Explants
Published 2022-01-01“…The cotyledonary nodes of six Kenyan adapted groundnuts genotypes (ICGV 12991, CG 7, Red Valencia, ICGV 90704, Chalimbana, and JL 24) were transformed using Agrobacterium tumefaciens strain EHA 101 carrying PNOV-IPT binary vector containing an IPT gene, which was driven by SARK promoter and terminated by TNOS terminator. The vector also contained the phosphomannose isomerase (PMI) gene for the selection of transformed tissues. …”
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289
ParSite is a multicolor DNA labeling system that allows for simultaneous imaging of triple genomic loci in living cells.
Published 2025-01-01“…The ParSite permits tracking promoter and terminator dynamics of the APP gene, which spans 290 kilobases in length. …”
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290
Overexpression of the Synthetic Chimeric Native-T-phylloplanin-GFP Genes Optimized for Monocot and Dicot Plants Renders Enhanced Resistance to Blue Mold Disease in Tobacco (N. taba...
Published 2014-01-01“…The synthetic chimeric gene (native-phylloplanin-GFP) was placed between the modified Mirabilis mosaic virus full-length transcript promoter with duplicated enhancer domains and the terminator sequence from the rbcSE9 gene. The chimeric gene, expressed in transgenic tobacco, was stably inherited in successive plant generations as shown by molecular characterization, GFP quantification, and confocal fluorescent microscopy. …”
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291
Improved constructs for bait RNA display in a bacterial three-hybrid assay
Published 2025-01-01“…In this work, we introduce new bait RNA constructs to (1) insulate the folding of individual components of the hybrid RNA with GC clamps and (2) express bait RNAs that do not encode their own intrinsic terminator. We find that short GC clamps (5 or 7 bp long) are more effective than a longer 13 bp GC clamp in the B3H assay. …”
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292
Evaluation of various RNA-seq approaches for identification of gene outrons in the flatworm Opisthorchis felineus
Published 2020-12-01“…One is based on the enzymatic elimination of overrepresented cDNAs, the other utilizes exonucleolytic degradation of uncapped RNA by Terminator enzyme. By using the outron-targeting methods, we were not able to obtain the enrichment of RNA preparations by processed outrons, which is most likely indicative of a rapid turnover of these trans-splicing intermediate products. …”
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293
Identification of <i>Tinomiscium petiolare</i> from Vietnam using the DNA barcode
Published 2021-07-01“…The purified PCR products were sequenced on the ABI 3500 Genetic Analyzer system, using BigDye®Terminator v3.1 Cycle Sequencing Kit. These genetic sequences were analyzed and compared, and a phylogenetic tree was constructed using BioEdit, BLAST, and MEGA 6 programs.Results and conclusion. …”
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294
Agricultural law /
Published 2016Table of Contents: “…Agricultural land tenure and protective legislation -- Land use and the law of the common agricultural policy -- Farm business tenancies -- Termination of farm business tenancies -- Agricultural holdings -- Agricultural holdings : the tenancy agreement -- Agricultural holdings : security of tenure -- Statutory succession to gricultural holdings -- Agricultural holdings : compensation on termination of tenancy -- Market gardens, smallholdings and allotments -- Farm cottages -- Planning law and development control -- Agriculture, nature conservation and land use -- Pollution control and land use -- Land use and the common agricultural policy.…”
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295
Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album
Published 2022-01-01“…The proteinase K gene was inserted into pPICZα-A in such a way that – at a subsequent stage of transfection into yeast cells – it was efficiently expressed under the control of the promoter and terminator of the AOX1 gene, and the product of the exogenous gene contained the signal peptide of the Saccharomyces cerevisiae a-factor to ensure the protein’s secretion into the culture medium. …”
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296
Disproportionate Distribution of HBV Genotypes A and D and the Recombinant Genotype D/E in the High and Low HBV Endemic Regions of Uganda: A Wake-Up Call for Regional Specific HBV...
Published 2022-01-01“…Automated DNA sequencing was performed using BigDye Terminator v3.1 Cycle Sequencing Kit on 3130 Genetic Analyzer (Applied Biosystems, USA). …”
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297
Molecular Epidemiology, Drug-Resistant Variants, and Therapeutic Implications of Hepatitis B Virus and Hepatitis D Virus Prevalence in Nigeria: A National Study
Published 2025-01-01“…We assessed 732 HBV DNA-extracted samples with detectable viral loads (VLs) for (sub-)genotypes and variants by whole-genome pre-amplification, nested PCR of the <i>s</i>-and <i>pol</i>-gene, and BigDye Terminator sequencing. We conducted HDV serology. In total, 19 out of the 36 + 1 states in Nigeria had a high prevalence of HBV (≥8%), with the highest prevalence (10.4%) in the north-central geopolitical zone. …”
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298
Optimization of Port Emergency Materials Dispatch with Time Windows Constraints under Uncertainties
Published 2021-01-01“…For example, at the beginning of 2020, the sudden outbreak of COVID-19 seriously affected terminal production and increased the short-term pressure of handling at container terminals. …”
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299
Novel Insights into Surface Energies and Enhanced Gas-Sensing Capabilities of ZnGa<sub>2</sub>O<sub>4</sub>(111) via Ab Initio Studies
Published 2025-01-01“…It explores the interactions of gas molecules such as NO, NO<sub>2</sub>, and CH<sub>3</sub>COCH<sub>3</sub> with Ga-terminated, O-terminated, and Ga-Zn-O-terminated surfaces. …”
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300
Dynamic optimization of a two-stage fractional system in microbial batch process
Published 2024-12-01“…The objective function was the productivity of 1,3-propanediol at the terminal time, while the decision variables were the initial concentrations of biomass and glycerol, and the terminal time of the batch process. …”
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