Depletion of myeloid-derived suppressor cells sensitizes murine multiple myeloma to PD-1 checkpoint inhibitors by Qiang Wang, Qing Yi, Wei Xiong, Rui Duan, Jianfei Qian, Liuling Xiao, Miao Xian, Pan Su, Chuanchao Zhang, Yabo Li, Ling Zhong, Chengyun Zheng

“…Total mouse IgG or Ig2b ELISA or QuickGel split beta SPE kits and in vivo bioluminescent imaging were used to monitor MM tumor burden. Cytometry by time of flight (CyTOF) was used to quantify MM TME components. …”
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Mechanism of Delaying Cartilage Degeneration in Knee Osteoarthritis with Rongjin Niantong Recipe Based on lncRNA NEAT1 and Nrf2/ARE Pathway by FU Changlong, XIE Xinyu, QIU Zhiwei, HUANG Yanfeng, JIN Linglu, LI Xihai

“…After the intervention, all groups were sacrificed under anesthesia, and the right knee tissues were separated and collected; HE staining was used to observe the pathological changes; Western blot was used to detect the expression levels of Nrf2, heme oxygenase-1 (HO-1), NAD (P) H quinone oxidoreductase-1 (NQO-1), kelch like ECH-associated protein 1 (Keap1) and inducible nitric oxide synthase (iNOS) in cartilage; Real-time PCR was used to detect the expression of lncRNA NEAT1 in cartilage tissue.Results1) Pathological changes of cartilage tissue: the cartilage matrix was light red and uniform, with complete tissue structure and clear tissue margins of each layer in the blank group; in the model group, the chondrocytes grew in clusters with thinner tangent layer. …”
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Nanotechnology-based herbal medicine: Preparation, synthesis, and applications in food and medicine by Farhang H. Awlqadr, Kithar Rasheed Majeed, Ammar B. Altemimi, Arkan Mohammed Hassan, Syamand Ahmed Qadir, Mohammed N. Saeed, Aryan Mahmood Faraj, Tablo H. Salih, Alaa Jabbar Abd Al‐Manhel, Mazin A.A. Najm, Efstathia Tsakali, Jan F.M. Van Impe, Ahmed A. Abd El-Maksoud, Tarek Gamal Abedelmaksoud

“…Nanotechnology has used such reinforcing materials to improve the performance of protective food packages and, consequently, ensure their longer service time. On the one hand, antimicrobial and antioxidant herbal nanoparticles will prevent spoilage and degradation during storage.On the other hand, nanoemulsions form a medium for vitamins, minerals, and antioxidants to be bioavailable in fortified meals. …”
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Global comparison of the economic costs of coronary heart disease: a systematic review and meta-analysis by Lan Gao, Susan Brumby, Crystal Man Ying Lee, George Mnatzaganian, Suzanne Robinson, Helen Brown, Rachel Huxley, Anna Peeters, Dan Xu, James Boyd, Sean Randall, Adrienne O’Neil, Sangita Shakya, Sarah Gauci, Anita Shrestha, James Lucas

“…Exploring advanced costing methods such as time-driven activity-based costing can optimise resource allocation and identify opportunities to reduce unnecessary costs and cost disparities.PROSPERO registration number CRD42023412044.…”
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Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves by Letiele Bruck de Souza, Amanda Leitão Gindri, Thainara de Andrade Fortes, Thais Felli Kubiça, Jefferson Enderle, Rafael Roehrs, Sidnei Moura e Silva, Vanusa Manfredini, Elton Luís Gasparotto Denardin

“…Our studies demonstrated for the first time that C. nutans crude leaf extract has high antioxidant capacity both in vitro and in vivo through different analysis techniques. …”
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Genomic changes during the evolution of the Coxiella genus along the parasitism-mutualism continuum by Santos-Garcia, Diego, Morel, Olivier, Henri, Hélène, El Filali, Adil, Buysse, Marie, Noël, Valérie, McCoy, Karen D., Gottlieb, Yuval, Klasson, Lisa, Zenner, Lionel, Duron, Olivier, Vavre, Fabrice

“…This antiporter might be involved in alkali resistance and the development of the resistant form that is able to persist in the environment for long periods of time. The Sha operon is eroded or absent in Coxiella-LEs. …”
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Pembrolizumab, radiotherapy, and an immunomodulatory five-drug cocktail in pretreated patients with persistent, recurrent, or metastatic cervical or endometrial carcinoma: Results... by Emiel A. De Jaeghere, Sandra Tuyaerts, An M. T. Van Nuffel, Ann Belmans, Kris Bogaerts, Regina Baiden-Amissah, Lien Lippens, Peter Vuylsteke, Stéphanie Henry, Xuan Bich Trinh, Peter A. van Dam, Sandrine Aspeslagh, Alex De Caluwé, Eline Naert, Diether Lambrechts, An Hendrix, Olivier De Wever, Koen K. Van de Vijver, Frédéric Amant, Katrien Vandecasteele, Hannelore G. Denys

“…Health-related quality of life was generally stable over time. Responders had a significantly higher proportion of peripheral T cells when compared to nonresponders (p = 0.013). …”
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Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste by Belén Heredia, Pablo Diaz Recio, Brian Jonathan Young, Pablo Pacheco, Franco Barcelo Zungre, Silvina Hruby, Maria Andreína Acevedo, María Inés Mercado, Gonzalo Roqueiro

“…A peak of the enzymatic activity of guaiacol peroxidase, ascorbate peroxidase and catalase enzymes in P. flexuosa seedlings showed a negative relationship between metal(loid) concentration and exposure time. After a drop in the enzymatic activity, an increase in the malondialdehyde content (lipid peroxidation) was observed. …”
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Exploring the mechanisms of Devonian oceanic anoxia: impact of ocean dynamics, palaeogeography, and orbital forcing by J. Gérard, L. Sablon, J. J. C. Huygh, A.-C. Da Silva, A. Pohl, C. Vérard, M. Crucifix

“…Furthermore, multistability and important hysteresis (particularly slow ocean time response) offer different mechanisms to account for the prolonged duration of some ocean anoxic events. …”
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Probiotics in the Management of Chronic Bacterial Prostatitis Patients: A Randomized, Double-Blind Trial to Evaluate a Possible Link Between Gut Microbiota Restoring and Symptom Re... by Cristina Vocca, Diana Marisol Abrego-Guandique, Erika Cione, Vincenzo Rania, Gianmarco Marcianò, Caterina Palleria, Luca Catarisano, Manuela Colosimo, Gregorio La Cava, Italo Michele Palumbo, Giovambattista De Sarro, Tommaso Ceccato, Simone Botti, Tommaso Cai, Alessandro Palmieri, Luca Gallelli

“…<i>Lactobacillus casei</i> DG^® induced a significantly (<i>p</i> < 0.01) faster recovery of symptoms than placebo (2 days vs. 8 days) and an increased time free from symptoms (86 days vs. 42 days) without the occurrence of adverse events. …”
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Health-related quality of life and health state utility value in idiopathic pulmonary fibrosis: a systematic review and meta-analysis by Guixiang Zhao, Siyuan Lei, Ya Li, Zhenzhen Feng, Jiansheng Li

“…Methods We searched the PubMed, EMBASE, Web of Science and Cochrane Library databases for studies reporting the HRQoL or HSUV of IPF patients, with the retrieval time from the establishment of each database to April 2024. …”
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Altered AKT1 and MAPK1 Gene Expression on Peripheral Blood Mononuclear Cells and Correlation with T-Helper-Transcription Factors in Systemic Lupus Erythematosus Patients by Sonia Garcia-Rodriguez, Jose-Luis Callejas-Rubio, Norberto Ortego-Centeno, Esther Zumaquero, Raquel Ríos-Fernandez, Salvador Arias-Santiago, Pilar Navarro, Jaime Sancho, Mercedes Zubiaur

“…Kinases have been implicated in the immunopathological mechanisms of Systemic Lupus Erythematosus (SLE). v-akt murine-thymoma viral-oncogene-homolog 1 (AKT1) and mitogen-activated-protein-kinase 1 (MAPK1) gene expressions in peripheral mononuclear cells from thirteen SLE patients with inactive or mild disease were evaluated using quantitative real-time reverse-transcription polymerase-chain-reaction and analyzed whether there was any correlation with T-helper (Th) transcription factors (TF) gene expression, cytokines, and S100A8/S100A9-(Calprotectin). …”
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Evaluasi Portal Berita Online pada Aspek Usability Menggunakan Heurtistic Evaluation dan Think Aloud by Putu Tantri Fajarini, Ni Kadek Ayu Wirdiani, I Putu Arya Dharmaadi

“…This research uses 3 news portals that provide a variety of news that occurs both locally, nationally, and internationally, in real time and quickly. Usability evaluation in this study used 20 research respondents who were selected using simple random sampling from readers and news portal staff who were evaluated. …”
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Role of PI3K/AKT/mTOR signaling pathway and sirtuin genes in chronic obstructive pulmonary disease development by G. F. Korytina, L. Z. Akhmadishina, V. A. Markelov, Y. G. Aznabaeva, O. V. Kochetova, T. R. Nasibullin, A. P. Larkina, N. N. Khusnutdinova, N. Sh. Zagidullin, T. V. Victorova

“…SNPs of SIRT1 (rs3758391, rs3818292), SIRT3 (rs3782116, rs536715), SIRT6 (rs107251), AKT1 (rs2494732), PIK3R1 (rs10515070, rs831125, rs3730089), MTOR (rs2295080, rs2536), PTEN (rs701848, rs2735343) genes were genotyped by real-time polymerase chain reaction (PCR) among 1245 case and control samples. …”
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YHSeqY3000 panel captures all founding lineages in the Chinese paternal genomic diversity database by Mengge Wang, Shuhan Duan, Qiuxia Sun, Kaijun Liu, Yan Liu, Zhiyong Wang, Xiangping Li, Lanhai Wei, Yunhui Liu, Shengjie Nie, Kun Zhou, 10K_CPGDP consortium, Yongxin Ma, Huijun Yuan, Bing Liu, Lan Hu, Chao Liu, Guanglin He

“…Objectives This study aimed to develop the highest-resolution Y-targeted sequencing panel, utilizing time-stamped, core phylogenetic informative mutations identified from high-coverage sequences in the YanHuang cohort. …”
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Open-label dose-extending placebos for opioid use disorder: a protocol for a randomised controlled clinical trial with methadone treatment by Annabelle M Belcher, Thomas O Cole, Aaron D Greenblatt, Stephen W Hoag, David H Epstein, Michael Wagner, Amy S Billing, Ebonie Massey, Kristen R Hamilton, Zofia K Kozak, Christopher J Welsh, Eric Weintraub, Emerson M Wickwire, Eric D Wish, Ted J Kaptchuk, Luana Colloca

“…Throughout this study time period, methadone dosages will be adjusted by an addiction clinician blind to patient assignment, per standard clinical methods. …”
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High-accuracy crRNA array assembly strategy for multiplex CRISPR by Xiangtong Zhao, Lixian Yang, Peng Li, Zijing Cheng, Yongshi Jia, Limin Luo, Aihong Bi, Hanchu Xiong, Haibo Zhang, Hongen Xu, Jinrui Zhang, Yaodong Zhang

“…Here, we present a novel, highly accurate, cost-, and time-saving strategy for CRISPR array assembly. Using this strategy, we efficiently assembled 12 CRISPR RNAs (crRNAs) (for AsCas12a) and 15 crRNAs (for RfxCas13d) in a single reaction. …”
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Safety and efficacy of intraoperative radiation therapy using a low-energy X-ray source for resectable pancreatic cancer: an interim evaluation of an ongoing prospective phase II s... by Xingyun Chen, Shuo Li, Chuntao Gao, Wei Wang, Haorui Li, Yuxiao Liu, Rui Liu, Jihui Hao

“…The primary endpoint was time to treatment failure (TTF) survival, whereas the secondary endpoints were safety and overall survival (OS). …”
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Predicted impact of banning nonessential, energy-dense food and beverages in schools in Mexico: A microsimulation study. by Ana Basto-Abreu, Martha Carnalla, Francisco Reyes-Sánchez, Alan Reyes-García, Michelle M Haby, Isabel Junquera-Badilla, Lianca Sartoris-Ayala, Juan A Rivera, Barry M Popkin, Tonatiuh Barrientos-Gutiérrez

“…Study limitations include assuming that obesity and the contribution of NEDFBs consumed at school remain constant over time, assuming full compliance, and not considering the potential effect of banning NEDFBs in stores near schools.…”
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Fhl1, a new spatially specific protein, regulates vein graft neointimal hyperplasia by Chaoqun Wang, Jiantao Chen, Zicong Feng, Bohao Jian, Suiqing Huang, Kangni Feng, Haoliang Liu, Zhuoming Zhou, Ziyin Ye, Jing Lu, Mengya Liang, Zhongkai Wu

“…Methods In this study, Sprague–Dawley (SD) rats were used to establish an external jugular vein transplantation model, and the NIH pathophysiological process was tracked across 11 time points (0–35 days) using various histological stains. …”
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