An automated platform for simultaneous, longitudinal analysis of engineered neuromuscular tissues for applications in neurotoxin potency testing by Jacob W. Fleming, Molly C. McCloskey, Kevin Gray, David R. Nash, Vincent Leung, Christos Michas, Shawn M. Luttrell, Christopher Cavanaugh, Julie Mathieu, Shawn Mcquire, Mark Bothwell, David L. Mack, Nicholas A. Geisse, Alec S.T. Smith

“…Dose-response studies using botulinum neurotoxin showed loss of function in a dose- and time-dependent manner (EC50 − 0.11 ± 0.015 µg). Variability of the EC50 values between 2 different iPSC differentiations of both cell types and 2 users was less than 2 %. …”
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The factors impacting on Gleason score upgrading in prostate cancer with initial low Gleason scores by Tzu-Heng Huang, Wei-Ming Li, Hung-Lung Ke, Ching-Chia Li, Wen-Jeng Wu, Hsin-Chih Yeh, Yen-Chun Wang, Hsiang-Ying Lee

“…Conclusion: Smaller PV and elevated PSAD are associated with increased risk of GS upgrading, whereas lead-time bias is not. A cut-off value of PV < 38 ml and PSAD > 0.26 ng/ml2 were further identified to be associated with pathological GS upgrading.…”
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Comparing the emotional impact of the UK COVID-19 lockdown in very preterm and full-term born children: a longitudinal study by Zeyuan Sun, Zeyuan Sun, Laila Hadaya, Laila Hadaya, Marguerite Leoni, Marguerite Leoni, Paola Dazzan, Emily Simonoff, Serena J. Counsell, A. David Edwards, Chiara Nosarti, Chiara Nosarti, Lucy Vanes

“…The emotional impact of the COVID-19 lockdown on the child and the parent, measured by the CRISIS, was studied in relation to pre-existing mental health, assessed with the parent-rated Strengths and Difficulties Questionnaire (SDQ), evaluated before the CRISIS completion (mean time gap 15 months). Linear regression model comparisons were conducted to study the effects of COVID-19-related stressors on children's and parents' behavior, relationships and mental health.ResultsThere were no significant group differences in pre-existing SDQ internalizing/externalizing symptoms, child's emotions or parent's emotions during the COVID-19 lockdown. …”
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Expression of the NUP153 and YWHAB genes from their canonical promoters and alternative promoters of the LINE-1 retrotransposon in the placenta of the first trimester of pregnancy by V. V. Demeneva, E. N. Tolmacheva, T. V. Nikitina, E. A. Sazhenova, S. Yu. Yuriev, A. Sh. Makhmutkhodzhaev, A. S. Zuev, S. A. Filatova, A. E. Dmitriev, Ya. A. Darkova, L. P. Nazarenko, I. N. Lebedev, S. A. Vasilyev

“…However, the expression level of the NUP153 gene from the alternative LINE-1 promoter was 17 times higher in chorionic villi and 23 times higher in extraembryonic mesoderm than in lymphocytes (p &lt; 0.05). …”
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Surface water and flood-based agricultural systems: Mapping and modelling long-term variability in the Senegal river floodplain by Andrew Ogilvie, Cheickh Sadibou Fall, Ansoumana Bodian, Didier Martin, Laurent Bruckmann, Djiby Dia, Issa Leye, Papa Malick Ndiaye, Donissongou Dimitri Soro, Jean Homian Danumah, Jean-Claude Bader, Jean-Christophe Poussin

“…Statistical regression models are then developed to estimate flooded and cultivated areas based on upstream flow values since 1950 and analyse trends and exceedance probabilities over time. Results reveal extreme interannual variations in peak flooded areas, ranging from 30,000 ha and 720,000 ha between 1950 and 2022, while annual water modules fluctuate between 210 and 1460 m3/s. …”
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Elevated D-dimer on admission may predict poor prognosis in childhood influenza associated encephalopathy by Huizhen Wang, Lingkong Zeng, Xingfeng Cheng, Hui Li, Hong Zhang, Yuanmei Shi, Yong Zhang, Changjian Li

“…In the univariate analysis, the poor prognosis group compared with the clinically cured group showed a significant increase in the number of days of hospitalization (3.0 vs. 9.5 days, P < 0.001), high-sensitivity C-reactive protein (6.80 vs. 1.88 mg/L, P = 0.003), interleukin-6 (20.26 vs. 8.24 pg/mL, P = 0.001), interleukin-10 (11.75 vs. 4.72 pg/mL, P = 0.003), alanine aminotransferase (104.0 vs. 20.0 U/L, P = 0.011), aspartate azelotransferase (186.5 vs. 37.0 U/L, P = 0.003), serum albumin (37.99 vs. 40.76 g/L, P = 0.042), prothrombin time (13.2 vs. 11.4 s, P = 0.017), D-dimer (4.34 vs. 0.44 mg/L FEU, P < 0.001), peripheral blood CD19 B-cell count (35.11 vs. 32.75 cells/µL, P = 0.018), and cerebrospinal fluid chloride (126.82 vs. 125.50 mmol/L, P = 0.027) were statistically different in the above 11 indicators. …”
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Testosterone exacerbates neutrophilia and cardiac injury in myocardial infarction via actions in bone marrow by Elin Svedlund Eriksson, Marta Lantero Rodriguez, Bente Halvorsen, Inger Johansson, Anna K. F. Mårtensson, Anna S. Wilhelmson, Camilla Huse, Thor Ueland, Pål Aukrust, Kaspar Broch, Lars Gullestad, Brage Høyem Amundsen, Geir Øystein Andersen, Mikael C. I. Karlsson, Malin Hagberg Thulin, Alessandro Camponeschi, Dana Trompet, Ola Hammarsten, Björn Redfors, Jan Borén, Elmir Omerovic, Malin C. Levin, Andrei S. Chagin, Tuva B. Dahl, Åsa Tivesten

“…Post-hoc analysis of clinical trial data showed that neutrophilia was greater in men than women after reperfusion of first-time ST-elevation MI, and tocilizumab, an interleukin-6 receptor inhibitor, reduced blood neutrophil counts and infarct size to a greater extent in men than women. …”
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<i>Pleurotus ostreatus</i>: Nutritional Enhancement and Antioxidant Activity Improvement Through Cultivation on Spent Mushroom Substrate and Roots of Leafy Vegetables by Eirini-Maria Melanouri, Ilias Diamantis, Marianna Dedousi, Eleni Dalaka, Paraskevi Antonopoulou, Seraphim Papanikolaou, Ioannis Politis, Georgios Theodorou, Panagiota Diamantopoulou

“…Afterwards, mushroom cultivation was conducted in bags, where production characteristics like earliness (the time from substrate inoculation to first harvest) and biological efficiency (BE %, the ratio of fresh mushroom weight to dry substrate weight × 100) were examined. …”
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Development of a Russian version of the Minnesota low vision reading chart by I.R. Stepanets, A.N. Kulikov, S.A. Koskin, D.I. Zhilchuk

“…</p> <p> <b>Results</b>: the time taken to read a standardized short test sentence was approx. 3 s. …”
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Five-Year Prospective Follow-Up of Patients with Hepatitis C Virus Infection Treated with Direct-Acting Antiviral Agents by Zhang Y, Xia H, Fan L, Jiang L, Yang B, Wang F

“…The proportion of all participants with undetectable HCV-RNA exceeded 98% at all follow-up time points. Patients experienced virological relapse, HCC, death and disease progression with a cumulative AIRs of 1.03% (95% confidence interval [CI] 0.6– 1.5), 1.76% (95% CI 1.2– 2.3), 1.51% (95% CI 1.0– 2.0), and 5.81% (95% CI 4.8– 6.8), respectively. …”
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Assessment of Lower Limb Muscle Volume Using 3D Ultrasonography: Validity and Reliability Compared to MRI by Paul Ritsche, Romina Ledergerber, Michele Pansini, Francesco Santini, Oliver Faude

“…While MRI provides high accuracy, it is costly and time-consuming. Three-dimensional ultrasonography (3DUS) offers a more accessible alternative but requires rigorous validation due to setup dependent accuracy. …”
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Ten-year outcomes of repeat keratoplasty for optical indications by Victoria Grace Dimacali, Hon Shing Ong, Hon Shing Ong, Hon Shing Ong, Hon Shing Ong, Stephanie Shuang Lang, Hla Myint Htoon, Hla Myint Htoon, Howard Cajucom-Uy, Hui Chen Charmaine Chai, Hui Chen Charmaine Chai, Marcus Ang, Marcus Ang, Anshu Arundhati, Anshu Arundhati, Anshu Arundhati, Jodhbir S. Mehta, Jodhbir S. Mehta, Jodhbir S. Mehta

“…Cox multiple regression analysis showed male gender (p = 0.023), PK regraft (p = 0.003), regraft rejection (p = 0.003), and initial graft indications of pseudophakic bullous keratopathy (p = 0.005) and aphakic bullous keratopathy (p = 0.004) to be risk factors for regraft failure, while longer time to regraft was associated with decreased risk of failure (p = 0.013).ConclusionPerforming EK for failed optical PK or EK significantly improved regraft survival compared to repeat PK. …”
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The Development of pre-examination and triage management in dental clinics under public health events from the perspective of COVID-19 epidemic (从新型冠状病毒肺炎疫情看公共卫生事件下口腔门诊预检分诊管理发展)... by SHE Lin (沈琳), CHU Xiaoyu (楚小玉), GAO Yu (高宇)

“…The primary screening process and the tertiary screening plan, various emergency plans and the treatment process for special patients, strengthen the pre-inspection staff's study of prevention and control policies and regulations, and gradually form the dental clinic pre-examination score Diagnosis management system. At the same time, it is also seen that in the normalization of epidemic prevention and control for several years, the development of oral pre-examination and triage has become more standardized and more information technology has been used to help fight the epidemic. …”
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Motor Neuron Disease Systematic Multi-Arm Adaptive Randomised Trial (MND-SMART): a multi-arm, multi-stage, adaptive, platform, phase III randomised, double-blind, placebo-controlle... by Jeremy Chataway, Suvankar Pal, Charis Wong, Elizabeth Elliott, Jenna M Gregory, Siddharthan Chandran, Judith Newton, Shuna Colville, Maria Stavrou, Christopher J Weir, Nigel Stallard, Malcolm R Macleod, Michelle Steven, Richard Anthony Parker, Arpan R Mehta, Robert J Swingler, Mahesh K B Parmar, Rachel S Dakin, Jill Williamson

“…Using this novel trial design reduces time, cost and number of participants required to definitively (phase III) evaluate drugs and reduces exposure of participants to potentially ineffective treatments.Ethics and dissemination MND-SMART was approved by the West of Scotland Research Ethics Committee on 2 October 2019. …”
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Microbiota, parasitic infections and their relationship with nutritional status and neurocognitive functioning in children from Ecuador—Proyecto Guagua: research protocol for a cro... by Sandra Victoria Abril-Ulloa, Tannia Valeria Carpio-Arias, Renata Alejandra Alvarado-Barba, Cristina Gabriela Ríos-Romero, Pamela Vinueza-Veloz, Sueny Paloma Lima-dos-Santos, Sueny Paloma Lima-dos-Santos, Igor Eduardo Astudillo-Skliarova, Igor Eduardo Astudillo-Skliarova, Ruth Irene Arias-Gutiérrez, Manuel Pérez-Quintana, Henk-Jan Boele, Henk-Jan Boele, Henk-Jan Boele, María Fernanda Vinueza-Veloz

“…Parents and children were informed of their right to withdraw from the study at any time. The findings of “Proyecto Guagua” will be disseminated through open-access, peer-reviewed publications and presented at local and international scientific events.…”
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Application of nursing consultation in special outpatient service (护理咨询在特需门诊服务中的应用) by ZHANG Yongli (张永丽), MI Wei (米薇), SHU Baozhen (舒宝珍), YU Lei (于蕾), XING Hui (兴慧), WANG Jing (王静)

“…Results After the implementation of nursing consultation, the number of patient revisits, appointments and satisfaction rate were 57. 07 %, 70. 57 % and 100 % respectively, which were significantly higher than those before the implementation of nursing consultation(P＜0. 01). The time of consultation and guidance was significantly shortened (P＜0. 01). …”
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Improving community-based first response to out of hospital cardiac arrest (FirstCPR): protocol for a cluster randomised controlled trial by Sandra Ware, Blake Angell, Julie Redfern, Clara Chow, Cate Ferry, Lee Taylor, Christopher Semsarian, Adrian Bauman, Janet E Bray, Simone Marschner, Michael Nelson, Saurabh Kumar, Matthew Vukasovic, Kevin Lai, Paul M Middleton, Pramesh Kovoor, Garry Jennings, Sonali Munot, Andrew Coggins, Alan Robert Denniss, Sarah Khanlari, Ian Opperman

“…The study employs a novel approach to reaching community members via social and cultural groups, and the intervention aims to address commonly cited barriers to training including lack of availability, time and costs.Methods and analysis FirstCPR is a cluster randomised trial that will be conducted across 200 community groups in urban and regional Australia. …”
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Patient-reported quality of life and adherence outcomes after integrating exclusive liquid meal replacement in patients with head and neck cancer undergoing chemoradiation: results... by Luca F. Valle, Fang-I Chu, Xiaoyan Wang, Andrew Erman, Jackie Hernandez, Elizabeth Kaoh, Nicolas Edgar, Ann C. Raldow, Deborah J. Wong, Michael L. Steinberg, Amar U. Kishan, Robert K. Chin, John V. Hegde

“…Paired t-test or Wilcoxon signed-rank test were performed to assess for differences between scores at each follow-up time point and baseline.ResultsOf the 60 enrolled patients, 51/60 (85%) lost 5% of their pre-treatment body weight. …”
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Evaluation of Healthcare Outcomes of Patients Treated with 3D-Printed-Titanium and PEEK Cages During Fusion Procedures in the Lumbar Spine by Corso KA, Teferra AA, Michielli A, Corrado K, Marcini A, Lotito M, Smith C, Costa M, Ruppenkamp J, Wallace A

“…The mean index hospital cost was ~&dollar;40,000, LOS was ~3 days, and discharge status to home/home health was ~85% for both; surgery time was 267 minutes for PEEK and 280 minutes for 3D-printed-titanium. …”
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Lipids as key biomarkers in unravelling the pathophysiology of obesity-related metabolic dysregulation by Anis Adibah Osman, Siok-Fong Chin, Lay-Kek Teh, Noraidatulakma Abdullah, Nor Azian Abdul Murad, Rahman Jamal

“…This study was aimed to discern unique metabolite signatures in obese and lean individuals using liquid chromatography-mass spectrometry quadruple time-of-flight (LC-MS/Q-TOF), with the goal of elucidating their roles in obesity. …”
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