Detection of fusion events by RNA sequencing in FFPE versus freshly frozen colorectal cancer tissue samples by Maxim Sorokin, Maxim Sorokin, Maxim Sorokin, Maxim Sorokin, Vladimir Lyadov, Vladimir Lyadov, Vladimir Lyadov, Maria Suntsova, Marat Garipov, Anna Semenova, Natalia Popova, Egor Guguchkin, Rustam Heydarov, Marianna Zolotovskaia, Xiaowen Zhao, Qing Yan, Ye Wang, Evgeny Karpulevich, Anton Buzdin, Anton Buzdin, Anton Buzdin, Anton Buzdin

“…However, tumor biosamples are usually formalin-fixed paraffin-embedded (FFPE) tissue blocks where RNA is heavily degraded, which in theory may result in decreased efficiency of fusion detection. Here, for the first time, we compared the efficacy of gene fusion detection by RNAseq for matched pairs of freshly frozen in RNA stabilizing solution (FF) and FFPE tumor tissue samples obtained from 29 human colorectal cancer patients. …”
Get full text

أسلوب التعامل مع الخلافات الفقهية: مراجعة كتاب الموافقات للإمام الشاطبي. دار الكتب العلمية، ٢٠٠٥، ٥٨٠ صفحة، ISBN: ٢-٧٤٥١-١٥٣٠-٨، (غلاف صلب)... by Muhammad Najib Abdullah, Muhammad Nazir Alias, Syaryanti Hussin, Ibrahim Zakariyya Moosa

“…Initially, ijtihād was not a cause of conflict and division, but a means to define concepts that lead to the truth. However, over time the differences devolved into sectarian fanaticism and blind adherence to opinions, leading to discord within the Muslim community. …”
Get full text

Elliptic and Quadrangular Flow of Protons in the High-Baryon-Density Region by Shaowei Lan, Zuowen Liu, Like Liu, Shusu Shi

“…In addition to comparing the transverse momentum (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><msub><mi>p</mi><mi>T</mi></msub></semantics></math></inline-formula>), rapidity, and centrality dependence of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mi>v</mi><mn>4</mn></msub><mo>/</mo><msubsup><mi>v</mi><mrow><mn>2</mn></mrow><mn>2</mn></msubsup></mrow></semantics></math></inline-formula> between HADES data and model calculations, we also explore its time evolution and energy dependence across <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msqrt><msub><mi>s</mi><mrow><mi>N</mi><mi>N</mi></mrow></msub></msqrt><mo>=</mo></mrow></semantics></math></inline-formula> 2.4 to 4.5 GeV. …”
Get full text

System Evaluation of Buyang Huanwu Decoction in Treatment of Acute Ischemic Stroke by TANG Qiang, ZHANG Shiqiang, ZHU Luwen, TANG Yizhou, LI Hongyu

“…Patients with acute ischemic stroke with Qi deficiency and blood stasis syndrome differentiation in traditional Chinese medicine were included in the randomized controlled trials (RCTs), who were treated with Buyang Huanwu decoction. The retrieval time for each database was from January 1, 2015 to January 31, 2020. …”
Get full text

Artificial intelligence-enhanced electrocardiography improves the detection of coronary artery disease by Chi-Hsiao Yeh, Tsung-Hsien Tsai, Chun-Hung Chen, Yi-Ju Chou, Chun-Tai Mao, Tzu-Pei Su, Ning-I Yang, Chi-Chun Lai, Chien-Tzung Chen, Huey-Kang Sytwu, Ting-Fen Tsai

“…Utilizing 12-lead ECG datasets, the algorithm integrated features like time intervals, amplitudes, and slope between peaks, a total of 561 features, with the XGBoost model yielding the best performance. …”
Get full text

Hospital-Based Surveillance of Respiratory Viruses Among Children Under Five Years of Age with ARI and SARI in Eastern UP, India by Hirawati Deval, Mitali Srivastava, Neha Srivastava, Niraj Kumar, Aman Agarwal, Varsha Potdar, Anita Mehta, Bhoopendra Sharma, Rohit Beniwal, Rajeev Singh, Amresh Kumar Singh, Vivek Gaur, Mahima Mittal, Gaurav Raj Dwivedi, Sthita Pragnya Behera, Asif Kavathekar, Sanjay Prajapati, Sachin Yadav, Dipti Gautam, Nalin Kumar, Asif Iqbal, Rajni Kant, Manoj Murhekar

“…A total of 943 samples from children aged 1 to 60 months were tested using multiplex real-time PCR for respiratory viruses in cases of ARI and SARI. …”
Get full text

Selective dry cow therapy: Clinical field trial on prevention and cure of intramammary infections by R.E. Niemi, M. Hovinen, P.J. Rajala-Schultz

“…Farmers collected aseptic quarter milk samples for microbiological quantitative real-time PCR analysis 0 to 4 d before dry-off and 0 to 4 d after calving. …”
Get full text

Reproductive modes in populations of late-acting self-incompatible and self-compatible polyploid Ludwigia grandiflora subsp. hexapetala in western Europe by Stoeckel, Solenn, Becheler, Ronan, Portillo-Lemus, Luis, Harang, Marilyne, Besnard, Anne-Laure, Lassalle, Gilles, Causse-Védrines, Romain, Michon-Coudouel, Sophie, Park, Daniel J., Pope, Bernard J., Petit, Eric J., Barloy, Dominique

“…It shapes genetic diversity and its structure over time and space, which can be used to infer reproductive modes. …”
Get full text

Interrelationship between altered metabolites and the gut microbiota in people living with HIV with different immune responses to antiretroviral therapy by Xuebin Tian, Zhongyao Gao, Yiwen Xie, Xiangyun Lu, Yulong Zhao, Peng Yao, Mingqing Dong, Lifeng Yu, Nanping Wu

“…Additionally, in vitro assays using cell counting kit 8, flow cytometry, and quantitative real-time reverse transcription PCR were employed to assess the effects of the metabolites on cell viability, immune marker expression, and cytokine levels. …”
Get full text

Cultivating crayfish (Procambarus clarkii) significantly enhances the quantity and diversity of soil microorganisms: evidence from the comparison of rice-wheat and rice-crayfish ro... by Hui Xu, Hui Xu, Dan Wang, Dan Wang, Dan Wang, Xuguang Li, Xuguang Li, Jiajia Li, Jiajia Li, Yu Xu, Yu Xu, Zhiqiang Xu, Zhiqiang Xu

“…Integrated farming of rice (IFA), as a time-honored agricultural model, can effectively increase agricultural productivity and provide ecological benefits. …”
Get full text

Atmospheric corrosion of carbon and galvanized steel under high rainfall conditions by Ariel Arroyo Heredia, Hector David Agudelo Arias, Maycol F. Mena, Elcy Serna Copete, F.A. Vásquez, Nerly Liliana Mosquera, Edison Banguero Palacios, Reiner Palomino Lemus, J.A. Calderón, Samir Córdoba Machado

“…The results indicate that all study regions obtained the highest time of wetness category (τ5). The sulfur-containing pollution (SO2) was categorized as P0 (rural atmosphere) with average values of Pd ≤ 4 mg/m2 - day. …”
Get full text

Potential public health impacts of gonorrhea vaccination programmes under declining incidences: A modeling study. by Lin Geng, Lilith K Whittles, Borame L Dickens, Martin T W Chio, Yihao Chen, Rayner Kay Jin Tan, Azra Ghani, Jue Tao Lim

“…VoD had a smaller impact (12.04% averted cases (95% CrI: 7.12%-15.00%)), but was three times more efficient than VoA in terms of averted cases per dose. …”
Get full text

Chain-mediated effect of physical activity between Chinese language-based L2 motivational self-system and intended effort by Zixia Bu, Yu Liu, Yi Ba, Shan Jiang, Huijuan Liu, Jiyuan Li, Fuqiang Dong, Haohan Yu, Xindi Zhao

“…With the exception of ideal L2 self, PA had a significant enhancing effect on L2MSS, learning experience, and intended effort (F = 2.36, η²=0.05, ; F = 3.16, η²=0.04, ; F = 2.33, η²=0.05; P < 0.05), and was incrementally associated with ought-to l2 self, endeavor degree, and time spent (F = 4.23, η²=0.05, ; F = 7.21, η²=0.04, ; F = 5.93, η²=0.03; P < 0.01). …”
Get full text

An efficient measure for the isolation of chenodeoxycholic acid from chicken biles using enzyme-assisted extraction and macroporous resins refining by Biao Yang, Fangyun Lu, Pengpeng Li, Jingjing Ma, Jing Yang, Xinxiao Zhang, Mei Cheng, Wenjing Yu, Yao Chai, Ye Zou, Weimin Xu, Daoying Wang

“…The advantages of energy conservation, time saving, economy and environmental friendliness make the measure using enzyme-assisted extraction and macroporous resins refining a promising candidate for isolation of CDCA from chicken bile.…”
Get full text

Nucleated red blood cells for characterization of systemic inflammatory response syndrome in dogs by ARom Cho, Hyeona Bae, Youngju Kim, Yeseul Jeon, Rankyung Jung, Minji Kim, Minjeong Kang, Sumin Cha, Kyu‐Woan Cho, Dong‐In Jung, Jinho Park, DoHyeon Yu

“…The presence of circulating nRBCs, but not the reticulocyte count, at admission was predictive of death in dogs with SIRS at 2 weeks (P = .01, AUC: 0.729) and 4 weeks (P = .002, AUC: 0.731). The overall survival time was shorter in nRBC‐positive dogs (95% CI, 47.35‐113.90) than nRBC‐negative dogs (95% CI, 90.92‐135.55; P = .03). …”
Get full text

Les réseaux d’alimentation en eau des pentes de la Croix-Rousse et de la presqu’île de Lyon by Jules Ramona

“…It is also demonstrates for the first time in Lyon the existence of a hybrid lead-wood adduction system.The frequent presence of metal sockets under the outdoor spaces prompts us to consider the widespread use of this type of water supply in a public context. …”
Get full text

Intrathecal Clonidine versus Dexmedetomidine as Adjuvants to Hyperbaric Ropivacaine in Lower Limb Orthopaedic Surgeries: A Randomised Clinical Study by Priya Kishnani, Aiman Monaf, Kalpesh Pati, Jatin Patel, Sara Mary Thomas

“…Results: The mean for age in Group RD5 was 39.43±10.6 years and Group RC30 was 41.43±13.65 years. The time to S2 regression was longer in Group RD5 (136.10±8.10 min) compared to Group RC30 (102.67±7.69 min) (p<0.0001). …”
Get full text

L’infanticide féminin en question chez les Gaulois du Midi : l’apport des analyses ADN sur les nouveau-nés enterrés dans les habitats de l’âge du Fer by Bernard Dedet, Henri Duday, Philippe Gruat, Mélanie Pruvost

“…These perinatal burials in houses and their immediate surroundings, do not, however, appear to fill the gap represented by the overall absence of deceased persons from this age group within the necropolises of the region at this time. The significantly variable density of these findings, depending on the site, as well as the nearly total absence of infants of a few months of age, in both the houses and necropolises, suggest that other solutions must have existed, located both outside village limits, as well as outside of the cemeteries. …”
Get full text

SARS-CoV-2 infection and antibody seroprevalence in routine surveillance patients, healthcare workers and general population in Kita region, Mali: an observational study 2020–2021... by Ulla Ashorn, Per Ashorn, Nigel Klein, Samba O Sow, Camilla Ducker, Elaine Cloutman-Green, Dagmar Alber, Fadima Cheick Haidara, Juho Luoma, Laura Adubra, Henry Badji, Fatoumata Diallo, Rikhard Ihamuotila, Owen Martell, Uma U Onwuchekwa, Oumar Samaké, Awa Traore, Kevin Wilson, Yue-Mei Fan

“…Objective To estimate the degree of SARS-CoV-2 transmission among healthcare workers (HCWs) and general population in Kita region of Mali.Design Routine surveillance in 12 health facilities, HCWs serosurvey in five health facilities and community serosurvey in 16 villages in or near Kita town, Mali.Setting Kita region, western Mali; local health centres around the central (regional) referral health centre.Participants Patients in routine surveillance, HCWs in local health centres and community members of all ages in populations associated with study health centres.Main outcome measures Seropositivity of ELISA test detecting SARS-CoV-2-specific total antibodies and real-time RT-PCR confirmed SARS-CoV-2 infection.Results From 2392 routine surveillance samples, 68 (2.8%, 95% CI: 2.2% to 3.6%) tested positive for SARS-CoV-2 by RT-PCR. …”
Get full text

Superior suspensory complex of the shoulder reconstruction for acute and chronic acromioclavicular joint dislocations: the Queensland Unit for Advanced Shoulder Research 3-tunnel t... by Helen Ingoe, MBBS, FRCS Eng, MD, MSc, PGCert, Jashint Maharaj, MBBS, MPHTM, FRSPH, Nagmani Singh, MBBS, MS, Kathir Azhagan Stalin, MBBS, MS, MRCS, DNB, Kristine Italia, MD, FPOA, Roberto Pareyon, MD, Mohammad N. Jomaa, MD, Kenneth Cutbush, MBBS, FRACS, FAOrthA, Ashish Gupta, MBBS, MSc, FRACS

“…Results: Of 26 patients in this series, 7 (27%) were operated within 4 weeks of injury, 2 (8%) were revision cases, and 6 (23%) were lateral clavicle fractures. Mean time to surgery was 14 weeks (2-650). Mean postoperative scores with associated 95% confidence interval were Specific Acromioclavicular Score 87.38 (confidence interval 75.38-99.37), American Shoulder and Elbow Surgeons score 94.60 (87.85-101.35), Constant 79.47 (71.87-87.07), simple shoulder test 85.44 (72.34-98.54) and visual analog score 0.50 (−0.15 to 1.15). …”
Get full text