Cancer stem cells and tumor-associated macrophages as mates in tumor progression: mechanisms of crosstalk and advanced bioinformatic tools to dissect their phenotypes and interacti... by Francesco Verona, Sebastiano Di Bella, Roberto Schirano, Camilla Manfredi, Francesca Angeloro, Giulia Bozzari, Matilde Todaro, Matilde Todaro, Giuseppe Giannini, Giuseppe Giannini, Giorgio Stassi, Veronica Veschi

“…The “oncogenic” immune cells are mainly represented by B and T lymphocytes, NK cells, and dendritic cells. Among immune cells, macrophages exhibit a more plastic and adaptable phenotype due to their different subpopulations, which are characterized by both immunosuppressive and inflammatory phenotypes. …”
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Effects of in-ovo injection of Yu ping feng polysaccharides on growth performance, intestinal development, and immunity in broiler chickens by Li-Gang Xue, Tian-Kui Guo, Juan Wang, Yu-Quan Shan, Li Guo, Dong-Xing Zhang, Zhong Wei, Dan Wang

“…Moreover, the analysis of immune development revealed that their serum levels in the polysaccharide-injected groups were also increased, with the YPF groups exhibiting superior performance than the APS and ATR groups and encouraging the development of T and B lymphocytes in the spleen and peripheral blood mononuclear cells. …”
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Jianpi Yiqi Busui prescription alleviates myasthenia gravis by regulating Th17 through the TAK1/P38 MAPK/eIF-4E signaling pathway by Zhuming Chen, Jing Lu, Tianying Chang, Dongmei Zhang, Yibin Zhang, Miao Liu, Tong Wu, Peng Xv, Jian Wang

“…Flow cytometry was utilized to count Th17, Th1, Th2, and Treg cells in lymphocytes. ELISA and RT-qPCR were used to measure acetylcholine receptor antibody (AChR-Ab) and Th17-related cytokines, including IL-17, IL-21, IL-23, TNF-α, TGF-β, IL-1β, and IL-6. …”
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Study on the infection characteristics and pathogenesis of duck circovirus 1 in Cherry Valley meat ducks by Hanqing Li, Guige Xu, Hui Yan, Lei Bei, Mingrui Zhao, Ruihua Zhang, Yu Meng, Yanli Zhu, Liangmeng Wei, Zhijing Xie, Shijin Jiang

“…The contents of calcium, phosphorus and lymphocytes decreased while alanine aminotransferase and total bilirubin increased in the serum of sick ducks. …”
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Establishment and Evaluation of a Rat Model of Non-Puerperal Mastitis by YIN Yulian, MA Lina, TU Siyuan, CHEN Ling, YE Meina, CHEN Hongfeng

“…On the 7th day, ductal dilation, epithelial necrosis and detachment, and pronounced periductal plasma cell and lymphocyte (predominantly T-lymphocytes) infiltration were observed. …”
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Bispecific antibody-mediated redirection of NKG2D-CAR natural killer cells facilitates dual targeting and enhances antitumor activity by Congcong Zhang, Anja Waldmann, Winfried S Wels, Jasmin Röder, Anne Scherer, Malena Bodden, Jordi Pfeifer Serrahima, Anita Bhatti, Nina Müller, Pranav Oberoi

“…Background Natural killer group 2D (NKG2D) is an activating receptor of natural killer (NK) cells and other lymphocytes that mediates lysis of malignant cells through recognition of stress-induced ligands such as MICA and MICB. …”
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The absolute number of circulating Treg cells is reduced in difficult-to-treat RA patients and is ameliorated by low-dose IL-2 by Huanhuan Yan, Huanhuan Yan, Huanhuan Yan, Xiaoyu Zi, Xiaoyu Zi, Xiaoyu Zi, Huer Yan, Xiaoying Zhang, Xiaoying Zhang, Xiaoying Zhang, Jie Bai, Jie Bai, Jie Bai, Chong Gao, Xiaofeng Li, Xiaofeng Li, Xiaofeng Li, Caihong Wang, Caihong Wang, Caihong Wang

“…The absolute numbers of peripheral blood lymphocyte subsets were detected by flow cytometry (FCM) and serum cytokine levels were detected by flow cytometry bead array (CBA).ResultsThe absolute number of T, CD4+ T, and Treg cells in the D2T RA group was lower than that in the HC, new, and treated RA groups. …”
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Immunogenic dying cells elicit potent anti-tumor T cell immunity against lung metastasis and tumorigenesis by Min Hu, Xinyu Meng, Tong Wang, Yifan Wang, Xiaodong Chen, Dongliang Xu, Wei He, Hongjia Zhang, Wenzheng Guo, Bo Jing, Siwei Zhang, Jianhua Xu, Beibei Sun, Xueqian Sun, Tingting Liu, Na Ni, Tongtong Zhang, Wenwen Cui, Xiaoyu Wu, Liping Xia, Feng Yao, Fang Zhang, Jing Du, Jiong Deng

“…Results Immunization with these live but ICD dying tumor cells induced potent tumor-specific anti-tumor T cell immunity, which not only protected host from challenge by these tumor cells in prevention and therapy in mouse model of lung metastasis, but also prevented tumors development in Gprc5a-ko mouse model of lung tumorigenesis. The lymphocytes from lymph nodes and tumor tissues exhibited greatly enhanced activities of Th1 cells and M1 macrophages. …”
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Enabling next-generation engineered TCR-T therapies based on high-throughput TCR discovery from diagnostic tumor biopsies by Thomas Kuilman, Deborah S. Schrikkema, Jules Gadiot, Raquel Gomez-Eerland, Laura Bies, Julia Walker, Robbert M. Spaapen, Hanna Kok, Demi Houg, Milena Viyacheva, Yvonne B. Claassen, Manuel Saornil, Oscar Krijgsman, Bas Stringer, Huiwen Ding, Anou Geleijnse, Anne C. Meinema, Bianca Weissbrich, Melissa Lancee, Carmen G. Engele, Marianna Sabatino, Pei-Ling Chen, Kenneth Y. Tsai, James J. Mulé, Vernon K. Sondak, Jitske van den Bulk, Noel F. de Miranda, Inge Jedema, John G. Haanen, Jeroen W. J. van Heijst, Ton N. Schumacher, Carsten Linnemann, Gavin M. Bendle

“…Abstract Adoptive cell therapy with tumor-infiltrating lymphocytes (TIL) can mediate tumor regression, including complete and durable responses, in a range of solid cancers, most notably in melanoma. …”
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Extracellular HMGB1 blockade inhibits tumor growth through profoundly remodeling immune microenvironment and enhances checkpoint inhibitor-based immunotherapy by Coraline Radermecker, Pascale Hubert, Patrick Roncarati, Stephanie Demoulin, Charlotte Pilard, Marie Ancion, Celia Reynders, Thomas Lerho, Diane Bruyere, Alizee Lebeau, Margot Meunier, Marie-Julie Nokin, Elodie Hendrick, Olivier Peulen, Philippe Delvenne, Michael Herfs

“…Indeed, without affecting the global number of (CD45+) immune cells, drastic reductions of monocytic/granulocytic myeloid-derived suppressor cells (MDSC) and regulatory T lymphocytes, a higher M1/M2 ratio of macrophages as well as an increased activation of both DC and pDC were continually observed following HMGB1 inhibition. …”
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Machine learning analysis of pathological images to predict 1-year progression-free survival of immunotherapy in patients with small-cell lung cancer by Hirotaka Matsumoto, Hiroaki Akamatsu, Nobuyuki Yamamoto, Yuki Sato, Daichi Fujimoto, Yoshihiko Taniguchi, Motohiro Tamiya, Yasuhiro Koh, Junya Fukuoka, Hisashi Tanaka, Naoki Furuya, Ryota Shibaki, Tsukasa Nozawa, Akira Sano, Yuka Kitamura, Takashi Kijima, Toshihide Yokoyama, Satoru Miura, Akito Hata, Jun Sugisaka

“…The machine learning analysis of the TIME had better accuracy than the human count evaluations (AUC of human count, CD8-positive lymphocyte: 0.681, FoxP3-positive lymphocytes: 0.626, PD-L1 score: 0.567).Conclusions The spatial analysis of the TIME using machine learning predicted the immunotherapy efficacy in patients with SCLC, thus supporting its role as an immunotherapy biomarker.…”
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Effect of neoadjuvant chemotherapy on the immune microenvironment in gastric cancer as determined by multiplex immunofluorescence and T cell receptor repertoire analysis by Jian Zhang, Xiaomei Li, Ying Hu, Bin Dong, Yongning Jia, Ziyu Li, Xiaojing Cheng, Jinyao Shi, Jiafu Ji, Zhongwu Li, Hong Du, Ting Guo, Shuqin Jia, Xiaofang Xing, Yunsheng Dou

“…Pre-NAC FOXP3+ T reg cells level was much richer in the response group and decreased more significantly in the stromal compartment. CD8+ cytotoxic T lymphocytes levels were elevated after chemotherapy, which was more significant in the group treated with XELOX regimen and in patients with better response, consistent with the TCR diversity elevation.Conclusions These findings have deepened our understanding of the immune modulating effect of chemotherapy and suggest that the immune profile of specimens after standard chemotherapy should be considered for the personalized immunotherapy to ultimately improve clinical outcome in patients with GC.…”
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Predictors of cytomegalovirus infection in patients with connective tissue disease treated by pulsed methylprednisolone therapy: a multicenter retrospective cohort study by Haruka Ito, Takahiko Yoshimoto, Akatsuki Kokaze, Kuninobu Wakabayashi, Kazuteru Noguchi, Kiyoshi Matsui, Bunki Natsumoto, Keishi Fujio, Yutaro Hayashi, Yuko Kaneko, Takahisa Gono, Koh Okamoto, Shu Okugawa, Kyoji Moriya, Hirohiko Sueki

“…The areas under the receiver operating characteristic curves for lymphocytes, NLR, and PLR were 0.742, 0.693, and 0.673 respectively. …”
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Gambaran Laboratorium Leukemia Kronik di Bagian Penyakit Dalam RSUP Dr. M. Djamil Padang by Muthia Rendra, Rismawati Yaswir, Akmal M Hanif

“…The bone marrow are hypercellular, compressing erythropoietic, myelopoietic hyperactivity and thrombopoietic mostly normal in number. Chronic lymphocytic leukemia consisting of 1 case shows the laboratory findings are moderate anemia, leukocytosis, thrombocytopenia, the morphology of erythrocyte is normochromic anisocytosis, leukocytes increase in number, increase in the number of lymphocytes, presentations smudge cells, and blast presentation is found in the peripheral blood, but the cellularity not be assessed.…”
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Coronavirus Disease 2019-Related Multisystem Inflammatory Syndrome in Children: A Systematic Review and Meta-Analysis by Ji-Gan Wang, Zhi-Juan Zhong, Meng Li, Jun Fu, Yu-Heng Su, You-Min Ping, Zi-Ji Xu, Hao Li, Yan-Hao Chen, Yu-Li Huang

“…The incidences of reduced lymphocytes, thrombocytopenia, hypoalbuminemia, elevated C-reactive protein, ferritin, LDH, interleukin-6, PCT, and FIB were 61.51%, 26.42%, 77.92%, 98.5%, 86.79%, 80.59%, 89.30%, 85.10%, and 87.01%, respectively. …”
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Key prognostic risk factors linked to poor functional outcomes in cerebral venous sinus thrombosis: a systematic review and meta-analysis by Lili Lin, Senfeng Liu, Wei Wang, Xiao-kuo He, Muhammad Hibatullah Romli, Ruthpackiavathy Rajen Durai

“…The blood biomarkers of NLR (log OR = 1.72, 95% CI 0.96–2.47), lymphocytes (Cohen’s d = -0.63, 95 CI -0.78—-0.47), and D-dimer (lnOR = 1.34, 95% CI 0.87–1.80) were the three most frequently reported factors. …”
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Chikungunya virus infection in the skin: histopathology and cutaneous immunological response by Natália Gedeão Salomão, Natália Gedeão Salomão, Luciana Araújo, Luiz José de Souza, Anna Luiza Young, Carlos Basílio-de-Oliveira, Rodrigo Panno Basílio-de-Oliveira, Jorge José de Carvalho, Priscilla Conrado Guerra Nunes, Juliana Fernandes da Silva Amorim, Douglas Valiati dos Santos Barbosa, Marciano Viana Paes, Kíssila Rabelo, Kíssila Rabelo, Flavia Dos Santos

“…Moreover, inflammatory infiltrates were composed of lymphocytes (CD4+ and CD8+) and macrophages (CD68+) in the dermis and perivascular infiltrate. …”
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Cell culture expansion media choice affects secretory, protective and immuno-modulatory features of adipose mesenchymal stromal cell-derived secretomes for orthopaedic applications by Enrico Ragni, Andrea Papait, Michela Maria Taiana, Paola De Luca, Giulio Grieco, Elsa Vertua, Pietro Romele, Cecilia Colombo, Antonietta Rosa Silini, Ornella Parolini, Laura de Girolamo

“…Secretomes effect was tested in in vitro models of chondrocytes, lymphocytes and monocytes to mimic the OA microenvironment. …”
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Work performance, physiological and hemato-biochemical responses in working buffalo with different load weights by Visoky Paján-Jiménez, Fernando D. Pazmiño-Rodríguez, Patricia Roldán-Santiago, Cristian Larrondo-Cornejo, Anna Dutro-Aceves, Luis A. de la Cruz-Cruz

“…Physiological (respiratory rate, pulse, heart rate, and rectal temperature) and biochemical (glucose, lactate dehydrogenase, creatine kinase, and total protein) parameters, as well as blood cell count (white blood cell, neutrophils, lymphocytes, monocytes, eosinophils, and basophils), increased after work regardless of weight load (p<0.001). …”
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Strength Training Session Induces Important Changes on Physiological, Immunological, and Inflammatory Biomarkers by Ayla Karine Fortunato, Washington Martins Pontes, Débora Maria Soares De Souza, Jéssica Santos Ferreira Prazeres, Lucas Soares Marcucci-Barbosa, Júlia Miranda Mól Santos, Érica Leandro Marciano Veira, Eduardo Bearzoti, Kelerson Mauro De Castro Pinto, André Talvani, Albená Nunes Da Silva

“…The number of white blood cells (WBC) and neutrophils increased in UTG and TG, post and 2 h after exercise. Lymphocytes increased postexercise but reduced 2 h after exercise in both groups, while the number of monocytes increased only immediately after the exercise session in UTG and TG. …”
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