Calprotectin and Calgranulin C as Biomarkers of Pancreatic Tumors: Baseline Levels and Level Changes after Surgery by Michal Holub, Eva Bartáková, Alžběta Stráníková, Eva Koblihová, Simona Arientová, Marie Blahutová, Jan Máca, Miroslav Ryska

“…Moreover, the serum levels of calprotectin and calgranulin C demonstrated a significant predictive value for the development of POPF; the predictive values of these two proteins were better than those of the serum level of C-reactive protein and the white blood cell count. Taken together, the results of this study suggest that calprotectin and calgranulin C serum levels are potential biomarkers for pancreatic tumors, surgical injury to the pancreatic tissue and the development of POPFs.…”
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Altered chromatin landscape and 3D interactions associated with primary constitutional MLH1 epimutations by Paula Climent-Cantó, Marc Subirana-Granés, Mireia Ramos-Rodríguez, Estela Dámaso, Fátima Marín, Covadonga Vara, Beatriz Pérez-González, Helena Raurell, Elisabet Munté, José Luis Soto, Ángel Alonso, GiWon Shin, Hanlee Ji, Megan Hitchins, Gabriel Capellá, Lorenzo Pasquali, Marta Pineda

“…Transcriptome (RNA-seq), chromatin landscape (ATAC-seq, H3K27ac CUT&Tag) and 3D chromatin interactions (UMI-4C) were studied in lymphoblastoid cell lines. The MLH1 promoter SNP (c.-93G > A, rs1800734) was used as a reporter in heterozygotes to assess allele-specific chromatin conformation states. …”
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Parent-of-origin effects on nuclear chromatin organization and behavior in a Drosophila model for Williams–Beuren Syndrome by A. V. Medvedeva, E. V. Tokmatcheva, A. N. Kaminskaya, S. A. Vasileva, E. A. Nikitina, A. V. Zhuravlev, G. A. Zakharov, O. G. Zatsepina, E. V. Savvateeva-Popova

“…Prognosis of neuropsychiatric disorders in progeny requires consideration of individual (1) parent-of-origin effects (POEs) relying on (2) the nerve cell nuclear 3D chromatin architecture and (3) impact of parent-specific miRNAs. …”
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Measurable residual disease as predictor of post–day +100 relapses after allografting in adult AML by Naveed Ali, Megan Othus, Eduardo Rodríguez-Arbolí, Corentin Orvain, Filippo Milano, Brenda M. Sandmaier, Chris Davis, Ryan S. Basom, Frederick R. Appelbaum, Roland B. Walter

“…Abstract: Measurable residual disease (MRD) by multiparametric flow cytometry (MFC) before allogeneic hematopoietic cell transplantation (HCT) identifies patients at high risk of acute myeloid leukemia (AML) relapse, often occurring early after allografting. …”
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The transcriptional response to yellow and wilt disease, caused by race 6 of Fusarium oxysporum f. sp. Ciceris in two contrasting chickpea cultivars by Aliakbar Faramarzpour, Sara Dezhsetan, Hamid Hassaneian Khoshro, Raheleh Mirdar Mansuri, Hamid Reza Pouralibaba, Zahra-Sadat Shobbar

“…., CaMYBs, CaGLK, CaERFs, CaZAT11-like, and CaNAC6) and cell wall integrity (e.g., CaPGI2-like, CaEXLs, CaCSLD and CaCYP73A100-like). …”
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MRI-based analysis of the microstructure of the thalamus and hypothalamus and functional connectivity between cortical networks in episodic cluster headache by Chiara Abagnale, Antonio Di Renzo, Giada Giuliani, Gabriele Sebastianelli, Francesco Casillo, Lucia Ziccardi, Vincenzo Parisi, Cherubino Di Lorenzo, Mariano Serrao, Francesca Caramia, Vittorio Di Piero, Gianluca Coppola

“…These differences may reflect changes in directional organization, cell size, and density.…”
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Comparative Analysis of the Safety and Functional Outcomes of Anterior versus Retropupillary Iris-Claw IOL Fixation by Paolo Mora, Giacomo Calzetti, Stefania Favilla, Matteo Forlini, Salvatore Tedesco, Purva Date, Viola Tagliavini, Arturo Carta, Rino Frisina, Emilio Pedrotti, Stefano Gandolfi

“…The following parameters were compared between the groups: best-corrected distance visual acuity (BCDVA), spherical and cylindrical refractive error, endothelial cell density (ECD), central macular thickness (CMT), and percentage and type of postoperative complications. …”
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Combination of Hotspot Mutations With Methylation and Fragmentomic Profiles to Enhance Multi‐Cancer Early Detection by Thi Hue Hanh Nguyen, Giang Hoang Vu, Tu Thi Nguyen, Tuan Anh Nguyen, Vu Uyen Tran, Luyen Thi Vu, Giang Thi Huong Nguyen, Nhat Duy Nguyen, Trung Hieu Tran, Van Thien Chi Nguyen, Thanh Dat Nguyen, Trong Hieu Nguyen, Dac Ho Vo, Thi Tuong Vi Van, Thanh Thi Do, Minh Phong Le, Le Anh Khoa Huynh, Duy Sinh Nguyen, Hung Sang Tang, Hoai‐Nghia Nguyen, Minh‐Duy Phan, Hoa Giang, Lan N. Tu, Le Son Tran

“…Method A targeted amplicon sequencing approach was developed to profile 700 hotspot mutations in cell‐free DNA and integrated into the SPOT‐MAS assay, creating a single‐blood draw workflow. …”
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Efficacy in prenatal vitamin B12 supplementation preventing vitamin B12 deficiency in exclusively breastfed infants by Eren Güzeloğlu, Bilge Doğan Taymur, Büşra Nükhet Pehlivanoğlu, Hüseyin Dağ

“…Introduction: Vitamin B12 is essential for erythropoiesis, cell proliferation, nucleoprotein and myelin synthesis, and normal growth and development of cognitive functions. …”
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Exogenous cystine increases susceptibility of drug-resistant Salmonella to gentamicin by promoting oxidation of glutathione metabolism and imbalance of intracellular redox levels by Junyuan Du, Junyuan Du, Zhiyi Wu, Zhiyi Wu, Chunyang Zhu, Chunyang Zhu, Heng Yang, Heng Yang, Feike Zhao, Feike Zhao, Binghu Fang, Binghu Fang

“…Exogenous cystine stimulated the metabolism of the cell and activated the glutathione pathway while altering the GSH/GSSG ratio, which placed bacteria in a state of redox imbalance with increased Fe2+ and ROS levels. …”
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Menthone lowers H3K27ac levels to inhibit Fusarium proliferatum growth by Li-Yan Zhang, Tian-Tian Li, Hong-Xin Liao, Jin-Rui Wen, Hong-Yan Nie, Fu-Rong Xu, Xiao-Yun Liu, Xian Dong

“…The treatment significantly inhibited hyphal growth, reduced spore germination rates from 31.49 to 4.95%, decreased spore viability from 46.88 to 20.91%, and reduced spore production by a factor of 17.92 compared with the control group while simultaneously enhancing cell membrane permeability. However, the direct relationship between menthone and histone acetylation in inhibiting F. proliferatum remains nebulous. …”
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Natural pigments: innovative extraction technologies and their potential application in health and food industries by Ayu Masyita, Gemala Hardinasinta, Ayun Dwi Astuti, Firdayani Firdayani, Dian Mayasari, Aki Hori, Ira Nur Ainun Nisha, Firzan Nainu, Takayuki Kuraishi

“…For example, delphinidin (30 µM) significantly inhibited the growth of three cancer cell lines (B16-F10, EO771, and RM1) by at least 90% after 48 h. …”
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Fertigation with alpha-tocopherol enhances morphological, physiological, and antioxidant responses in radish (Raphanus sativus L.) under drought stress by Muhammad Shahbaz Chishti, Muhammad Shahbaz, Muhammad Kaleem, Saba Shafi, Anam Mehmood, Zhang Qingzhu, Musarat Mansha, Nimra Shehzadi, Shamsa Rana, Hina Shahid, Abeer Hashem, Alanoud Alfagham, Elsayed Fathi Abd-Allah

“…At the same time, exogenous fertigation of α-Toc protects the membranes by reducing the level of LPX, enhancing antioxidants such as catalase (CAT) and peroxidase (POX) to scavenge the reactive oxygen species (ROS), and enhancing the osmolyte as total soluble proteins to maintain cell internal osmotic potential. Also, the α-Toc enhanced the photosynthetic pigments and significantly increased photosynthetic activity in the Early Milo (G2) as compared to Laal Pari (G1) genotype under drought, enhancing water use efficiency by maintaining transpiration rate and stomatal conductance. …”
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Astragalin: A Bioactive Phytochemical with Potential Therapeutic Activities by Ammara Riaz, Azhar Rasul, Ghulam Hussain, Muhammad Kashif Zahoor, Farhat Jabeen, Zinayyera Subhani, Tahira Younis, Muhammad Ali, Iqra Sarfraz, Zeliha Selamoglu

“…It carries out the aforementioned activities by the regulation and modulation of various molecular targets such as transcription factors (NF-κB, TNF-α, and TGF-β1), enzymes (iNOS, COX-2, PGE2, MMP-1, MMP-3, MIP-1α, COX-2, PGE-2, HK2, AChe, SOD, DRP-1, DDH, PLCγ1, and GPX), kinases (JNK, MAPK, Akt, ERK, SAPK, IκBα, PI3K, and PKCβ2), cell adhesion proteins (E-cadherin, vimentin PAR-2, and NCam), apoptotic and antiapoptotic proteins (Beclin-1, Bcl-2, Bax, Bcl-xL, cytochrome c, LC3A/B, caspase-3, caspase-9, procaspase-3, procaspase-8, and IgE), and inflammatory cytokines (SOCS-3, SOCS-5, IL-1β, IL-4, IL-6, IL-8, IL-13, MCP-1, CXCL-1, CXCL-2, and IFN-γ). …”
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Reduction effect of oral pravastatin on the acute phase response to intravenous zoledronic acid: protocol for a real-world prospective, placebo-controlled trial by Qi Liu, Min Zhang, Liyuan Tao, Chunli Song, Haiyan Li, Gengyu Han, Runting Li, Dongwei Fan, Guohong Du, Dongyang Liu

“…This process can decrease the accumulation of isopentenyl pyrophosphate to prevent γδT-cell activation and inflammatory factor production, blocking APR occurrence. …”
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Energetic and genomic potential for hydrogenotrophic, formatotrophic, and acetoclastic methanogenesis in surface-expressed serpentinized fluids of the Samail Ophiolite by Alta E. G. Howells, Alta E. G. Howells, Lilja M. Quinn, Lilja M. Quinn, Miguel G. Silva, Miguel G. Silva, Kylie Akiyama, Kylie Akiyama, Lucas M. Fifer, Lucas M. Fifer, Grayson Boyer, Srishti Kashyap, Kirt Robinson, Kirt Robinson, Jared Broddrick, Everett L. Shock, Tori M. Hoehler

“…In this study we quantified the impact of inorganic carbon, formate and acetate availability for methanogenic metabolisms, across a range of fluid chemistries, in terms of (1) the potential diffusive flux of substrates to the cell, (2) the Affinity (Gibbs energy change) associated with methanogenic metabolism, and (3) the energy “inventory” per kg fluid. …”
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The comprehensive assessment of epigenetics changes during skin development by Li Lei, Ling Jiang, Yibo Hu, Menglu Chen, Jinhua Huang, Jing Chen, Qinghai Zeng

“…However, the minimal erythema dose of UV exposure altered multiple epigenetic effectors regulating extracellular matrix organization, cell-matrix adhesion, innate immune response, mitochondrial function and mRNA processing. …”
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Anti-IL-5 treatment, but not neutrophil interference, attenuates inflammation in a mixed granulocytic asthma mouse model, elicited by air pollution by Joyceline De Volder, Annelies Bontinck, Valerie Haelterman, Louis Boon, Guy F. Joos, Guy G. Brusselle, Tania Maes

“…Outcome parameters included eosinophils subsets (homeostatic EOS and inflammatory EOS), proinflammatory cytokines, goblet cell hyperplasia and airway hyperresponsiveness. …”
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Phylogenetic analysis, metabolic profiling, and environmental adaptation of strain LCG007: a novel Rhodobacteraceae isolated from the East China Sea intertidal zone by Cuizhu Liang, Jiahua Wang, Jie Liu, Zekai Wang, Junwei Cao, Xi Yu, Li Zhang, Jiasong Fang, Jiasong Fang

“…This conservation was further supported by the significant impact of light on the growth and cell aggregation of strain LCG007, suggesting that acquirement of photosynthetic genes could play a crucial role in the speciation of their common ancestor. …”
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Comprehensive analysis of histophysiology, transcriptomics and metabolomics in goslings exposed to gossypol acetate: unraveling hepatotoxic mechanisms by Jun Yu, Jun Yu, Haiming Yang, Jian Wang, Zixin Huang, Shi Chen, Hongchang Zhao, Jun Wang, Zhiyue Wang

“…Overall, GA-induced hepatotoxicity involves impaired energy production, disrupted lipid metabolism, and abnormal liver focal adhesion, leading to liver cell dysfunction. These findings highlight the vulnerability of mitochondria and critical metabolic pathways, providing insights into the molecular mechanisms of GA toxicity and guiding future studies on mitigating GA-induced liver damage in goslings.…”
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