RNA interference of Aspergillus flavus in response to Aspergillus flavus partitivirus 1 infection by Yinhui Jiang, Yinhui Jiang, Xiang Liu, Xiang Liu, Xun Tian, Xun Tian, Jianhong Zhou, Jianhong Zhou, Qinrong Wang, Qinrong Wang, Bi Wang, Bi Wang, Wenfeng Yu, Wenfeng Yu, Yanping Jiang, Tom Hsiang, Xiaolan Qi, Xiaolan Qi

“…We also investigated the sensitivity of virus-free and AfPV1-infected RNAi mutants and the parental strain to cell wall stress, osmotic stress, genotoxic stress, and oxidative stress. …”
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Neoadjuvant Chemoradiotherapy Upregulates the Immunogenicity of Cold to Hot Tumors in Esophageal Cancer Patients by Yushi Nagaki, MD, PhD, Satoru Motoyama, MD, PhD, Yusuke Sato, MD, PhD, Akiyuki Wakita, MD, PhD, Hiromu Fujita, MD, PhD, Kohei Kemuriyama, MD, Ryohei Sasamori, MD, Shu Nozaki, MD, Kyoko Nomura, MD, PhD, Yoshihiro Minamiya, MD, PhD

“…To test the hypothesis that neoadjuvant chemoradiotherapy (NACRT) is more effective against hot esophageal squamous cell carcinoma (ESCC) and that it may upregulate tumor immunogenicity. …”
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Characterization and genomic analysis of a Herelleviridae bacteriophage UHP46 infecting mastitis-causing Staphylococcus aureus by Sara Najeeb, Imran Khan, Javed Muhammad, Muhammad Jahangir, Iqbal Ahmad Alvi, Anza Abbas, Aman Ullah, Arif Ullah, Wajiha Sajjad, Hashir Khan, Amjad Khan, Amjad Khan

“…One-step growth curve analysis indicated latent period of approximately 20 mins and burst size of about 27 progeny/cell. In organic stability test, UHP46 showed stability in DMSO and acetone. …”
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High serum CA19-9 predicts severe cholecystitis in calculous cholecystitis patients by Shouwen Wang, Dachen Zhou, Wanjin Chen, Qi Guo, Liujin Hou, Ruolin Wu, Wei Wang, Muhammad Annus Khan, Muhammad Ahmad, Fan Huang, Meijuan Zheng, Guobin Wang, Hongchuan Zhao, Xiaoping Geng, Xiaojun Yu

“…Perioperative data including blood cell count, inflammatory markers, liver function, imaging and operation-related parameters from these patients were collected for analysis and verified with second group of 105 calculous cholecystitis patients from second hospital. …”
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Application of 2D polymerase chain reaction for single-tube detection of high-risk human papillomaviruses by Weifeng Wang, Min Jiang, Ying Liu, Xuan Wu, Yan Chen, Xiaoyun Zhang, Weiwei Liu

“…A total of 294 cervical exfoliated cell samples were tested using 2D PCR and flow fluorescence hybridization method. …”
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Investigating the potential of diffusion tensor atlases to generate anisotropic clinical tumor volumes in glioblastoma patients by Kim Hochreuter, Gregory Buti, Ali Ajdari, Christopher P. Bridge, Gregory C. Sharp, Sune Jespersen, Slávka Lukacova, Thomas Bortfeld, Jesper F. Kallehauge

“…The degree of CTV anisotropy was controlled by the migration ratio, modeling tumor cell migration along the dominant white matter fiber direction extracted from DTI. …”
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Synthesis and Physico-Chemical Analysis of Dextran from Maltodextrin via pH Controlled Fermentation by <i>Gluconobacter oxydans</i> by Seung-Min Baek, Bo-Ram Park, Legesse Shiferaw Chewaka, Yun-Sang So, Ji-Hye Jung, Seul Lee, Ji Young Park

“…This study examined how jar fermentor conditions impact the growth and enzyme activity of <i>G. oxydans</i>, with a focus on the effects of pH on dextran synthesis via bioconversion (without pH control, pH 4.5, and pH 5.0; Jp-UC, Jp-4.5, and Jp-5.0). After 72 h, the cell density (O.D. at 600 nm) was 7.2 for Jp-4.5, 6.5 for Jp-5.0, and 3.7 for Jp-UC. …”
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Effects of GABA Supplementation on Intestinal SIgA Secretion and Gut Microbiota in the Healthy and ETEC-Infected Weanling Piglets by Yuanyuan Zhao, Jing Wang, Hao Wang, Yonggang Huang, Ming Qi, Simeng Liao, Peng Bin, Yulong Yin

“…In conclusion, GABA supplementation can enhance intestinal mucosal immunity by promoting jejunal SIgA secretion, which might be related with the T-cell-dependent pathway and altered gut microbiota structure and metabolism.…”
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Screening potential antileukemia ingredients from sweet potato: integration of metabolomics analysis, network pharmacology, and experimental validation by Lianling Xu, Lianling Xu, Kaixuan Zeng, Kaixuan Zeng, Zuoyue Duan, Zuoyue Duan, Jing Liu, Yan Zeng, Miao Zhang, You Yang, Qulian Guo, Yanling Jin, Wenjun Liu, Ling Guo

“…Then, an extract derived from these leaves was employed for in vitro experiments, demonstrating significant inhibition of AML cell growth. Subsequently, based on the results of metabolomics analysis and network pharmacology, cynaroside, nepitrin, and yuanhuanin were identified as potential antileukemia agents present in sweet potato for the first time; while CASP3, KDR, EGFR, and SRC were recognized as pivotal targets of these three monomers against AML. …”
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Clinical efficacy of electrosurgery and steel scalpel surgery for umbilical herniorrhaphy in bovine calves: A comparative analysis by Mohammad Raguib Munif, Md Ariful Islam, Md Sabuj Rahman, Md Mizanur Rahman, Md Rafiqul Alam

“…Hematobiochemical parameters, including total erythrocyte count, hemoglobin, packed cell volume, total serum protein, blood urea nitrogen, and creatinine, were also assessed at these intervals. …”
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Establishing of a risk prediction model for metabolic dysfunction-associated steatotic liver disease: a retrospective cohort study by Nan Li, Chenbing Liu, Zhangfan Lu, Wenjian Wu, Feng Zhang, Lihong Qiu, Chao Shen, Di Sheng, Zhong Liu

“…Results Our study indicated that male, body mass index (BMI), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), fasting plasma glucose (FPG), serum uric acid to creatinine ratio (SUA/Cr) and white blood cell (WBC) were associated with MASLD incidence. …”
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Genome-wide analysis of sugar transporter gene family in Erianthus rufipilus and Saccharum officinarum, expression profiling and identification of transcription factors by Sehrish Akbar, Xuiting Hua, Yingying Zhang, Gang Liu, Tianyou Wang, Huihong Shi, Zhen Li, Yiying Qi, Habiba Habiba, Wei Yao, Mu-Qing Zhang, Jisen Zhang

“…Sugar, the primary product of photosynthesis, is a vital requirement for cell activities. Allocation of sugar from source to sink tissues is facilitated by sugar transporters (ST). …”
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Development of a Recombinant Omicron BA.1 Subunit Vaccine Candidate in Pichia pastoris by Sibel Kalyoncu, Dogu Sayili, Ayca Zeybek Kuyucu, Hakan Soyturk, Seyda Gullu, Busra Ersayan, Ibrahim Oguzhan Tarman, Mehmet Ender Avci, Olcay Mert, Umut Haskok, Ege Tekin, Huseyin Akinturk, Ridvan Orkut, Aysegul Demirtas, Idil Tilmensagir, Ceren Ulker, Bilgi Gungor, Mehmet Inan

“…The long‐term immunological response in mice was measured by analyzing serum antibody titers and T‐cell response of splenocytes after 60 weeks. Interestingly, IgG titers and Th1 response were significantly high even after a year. …”
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The autoimmune disease risk variant NCF1-His90 is associated with a reduced risk of tuberculosis in women by Xinjun Hu, Shasha Li, Renliang Huang, Ziwei Fu, Chenyu Ma, Zheng Cheng, Hongjun Hu, Qiaomiao Zhou, Frank Petersen, Xinhua Yu, Xinhua Yu, Junfeng Zheng

“…Additionally, healthy individuals with the NCF1 rs201802880 AA genotype had significantly lower circulating white blood cell (5.56 ± 1.78 vs 6.43 ± 1.59, p=0.003) and neutrophil (3.23 ± 1.20 vs 3.74 ± 1.23, p = 0.02) counts compared to those with the GG or GA genotypes, with this difference being more pronounced in women than in men.ConclusionThis study demonstrates that the autoimmune disease-causal NCF1 variant is associated with a protective effect against TB infection.…”
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Aqueous humor metabolomic profiling identifies a distinct signature in pseudoexfoliation syndrome by Arturs Zemitis, Arturs Zemitis, Juris Vanags, Juris Vanags, Theresa Schiemer, Theresa Schiemer, Kristaps Klavins, Kristaps Klavins, Guna Laganovska, Guna Laganovska

“…These results suggest a potential link to ferroptosis.ConclusionFerroptosis is a form of programmed cell death characterized by iron-dependent LPO. The inhibition of the antiporter system Xc− leads to increased oxidative stress, suggesting that the changes seen in PEXS could be linked to ferroptosis. …”
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Hematologic and clinical chemistry reference intervals for six species of wild birds frequently rescued in the Republic of Korea by Myeongsu Kim, Myeongsu Kim, Zun Zun Wut Hmohn, Woochan Jang, Woochan Jang, Geonwoo Baek, Geonwoo Baek, Jae-Ik Han, Jae-Ik Han

“…The common kestrel exhibited significantly lower white blood cell counts and heterophil counts than other species. …”
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Early Transcriptional Changes Induced by Wnt/β-Catenin Signaling in Hippocampal Neurons by Eduardo Pérez-Palma, Víctor Andrade, Mario O. Caracci, Bernabé I. Bustos, Camilo Villaman, Matías A. Medina, Miguel E. Ávila, Giorgia D. Ugarte, Giancarlo V. De Ferrari

“…Main biological processes enriched with differentially expressed genes included neural precursor (GO:0061364, p-adjusted = 2.5 × 10−7), forebrain development (GO:0030900, p-adjusted = 7.3 × 10−7), and stem cell differentiation (GO:0048863 p-adjusted = 7.3 × 10−7). …”
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Biglycan-driven risk stratification in ZFTA-RELA fusion supratentorial ependymomas through transcriptome profiling by Konstantin Okonechnikov, David R. Ghasemi, Daniel Schrimpf, Svenja Tonn, Martin Mynarek, Jan Koster, Till Milde, Tuyu Zheng, Philipp Sievers, Felix Sahm, David T.W. Jones, Andreas von Deimling, Stefan M. Pfister, Marcel Kool, Kristian W. Pajtler, Andrey Korshunov

“…Multigene analysis disclosed 1892 survival-associated genes, and a metagene set of 100 genes subdivided ST-EPN ZFTA-RELA into favorable and unfavorable transcriptome subtypes composed of different cell subpopulations as detected by deconvolution analysis. …”
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A nucleoside-modified mRNA vaccine prevents enterovirus A71 infection in mouse model by Fengyu Chi, Fengyu Chi, Xu Zhang, Xu Zhang, Dong Zhang, Dong Zhang, Airu Zhu, Zhen Zhuang, Zhaoyong Zhang, Zhenjie Zhang, Chuansong Quan, Kaixiao Nie, Juan Li, Chunhong Yin, Jie Tong, Yuming Li, Yuming Li, Yuming Li

“…Passive protection was tested by transferring immune sera to neonatal mice challenged with EV-A71.ResultsThe VP1 mRNA-LNP vaccine elicited robust humoral and cellular immunity, including high levels of VP1-specific IgG, neutralizing antibodies, and a Th1-biased T-cell response. Notably, the mRNA vaccine outperformed the inactivated vaccine in eliciting cellular immunity. …”
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Recognition and cleavage of human tRNA methyltransferase TRMT1 by the SARS-CoV-2 main protease by Angel D'Oliviera, Xuhang Dai, Saba Mottaghinia, Sophie Olson, Evan P Geissler, Lucie Etienne, Yingkai Zhang, Jeffrey S Mugridge

“…We find that Mpro can cleave endogenous TRMT1 in human cell lysate, resulting in removal of the TRMT1 zinc finger domain. …”
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