Principles and medical applications of gene editing by CRISPR / Cas by Rolando Sánchez Artigas, María Teresa Díaz Armas, Raisa Rodríguez Duque, Pedro Enrique Miguel Soca

“…The introduction of the system into the cell interior occurs with plasmids, RNA or ribonucleoproteins. …”
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Chemical- and photo-activation of protein-protein thiol-ene coupling for protein profiling by André Campaniҫo, Marcin Baran, Andrew G. Bowie, Daniel B. Longley, Timothy Harrison, Joanna F. McGouran

“…When thiol-ene activation was performed in protein and cell lysate models, all three initiation methods were successful. …”
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Trend Analysis of Pakistan Railways Based on Industry Life Cycle Theory by Li Xuemei, Khalid Mehmood Alam, Shitong Wang

“…The main reasons behind the decline were underinvestment, political interference, and the rise of the same-sector competitor, the National Logistics Cell (NLC). From the year 2011, it experienced an upward trend of combined utility curve and showed a new round of industry life cycle. …”
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Pathologic complete response following low-dose radiation for advanced oral cavity cancer in a patient with human immunodeficiency virus by Amy B. Leming, Andrea L. Johnston, Greg A. Krempl, Evan J. Fowle, Daniel J. Morton, Christina E. Henson

“…Abstract Background Advanced squamous cell carcinoma (SCCa) of the oral cavity is often not amenable to curative-intent therapy due to tumor location, tumor size, or comorbidities. …”
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Light and nutrients modulate the temperature‐sensitivity of growth in phytoplankton by Vanessa Weber de Melo, Marc J.‐F. Suter, Anita Narwani

“…We explore how stoichiometry and cell physiology may explain the complex responses of phytoplankton to multiple environmental changes.…”
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Laser Photobiomodulation for a Complex Patient with Severe Hydroxyurea-Induced Oral Ulcerations by Marco Cabras, Adriana Cafaro, Alessio Gambino, Roberto Broccoletti, Ercole Romagnoli, Davide Marina, Paolo G. Arduino

“…Patients affected by polycythemia vera (PV), a myeloproliferative neoplasm characterized by an elevated red blood cell mass, are at high risk of vascular and thrombotic complications. …”
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Temperature-Responsive Gelation of Type I Collagen Solutions Involving Fibril Formation and Genipin Crosslinking as a Potential Injectable Hydrogel by Shunji Yunoki, Yoshimi Ohyabu, Hirosuke Hatayama

“…The elastic modulus of the 0.5% PSC/genipin gel system could be adjusted in a range of 2.5 to 50 kPa by the PSC and genipin concentrations, suggesting that a PSC/genipin solution is a potential injectable gel system for drug and cell carriers, with mechanical properties matching those of living tissues.…”
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Functional reconstruction of lower eyelid using paramedian forehead flap combined with frontalis muscle and periosteum by Riku Katayama, Takako Fujii, Chie Kanayama, Hisashi Sakuma

“…In this case report, we describe a two-stage reconstruction of a skin defect, including the orbicularis oculi muscle, after resection of a basal cell carcinoma using a paramedian forehead flap combined with the frontalis muscle and periosteum. …”
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Complement Activation and Inhibition in Wound Healing by Gwendolyn Cazander, Gerrolt N. Jukema, Peter H. Nibbering

“…Complement activation is needed to restore tissue injury; however, inappropriate activation of complement, as seen in chronic wounds can cause cell death and enhance inflammation, thus contributing to further injury and impaired wound healing. …”
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A Krukenberg Tumor from an Occult Intramucosal Gastric Carcinoma Identified during an Autopsy by Yoshiaki Nakamura, Ayako Hiramatsu, Takafumi Koyama, Yu Oyama, Ayuko Tanaka, Koichi Honma

“…Histopathological examination of the ovaries showed bilateral Krukenberg tumors with signet-ring cell carcinoma (SRCC). Extensive testing including upper and lower gastrointestinal endoscopy and whole-body imaging did not detect the primary lesion. …”
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In vitro evaluation of stearylamine cationic nanoemulsions for improved ocular drug delivery by Dukovski Bisera Jurišić, Bračko Ana, Šare Marija, Pepić Ivan, Lovrić Jasmina

“…Physicochemical characterization, stability profile, in vitro mucoadhesion study and biocompatibility study employing 3D HCE-T cell-based model of corneal epithelium pointed out the NE with 0.05 % (m/m) SA as the leading formulation. …”
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Severe Combined Immunodeficiency Disorder due to a Novel Mutation in Recombination Activation Gene 2: About 2 Cases by Ibtihal Benhsaien, Fatima Ailal, Khadija Elazhary, Jalila El bakkouri, Abdallah Badou, Ahmed Aziz Bousfiha

“…Affected children are highly susceptible to bacterial, viral, fungal, and opportunistic infections with life-threatening in the absence of hematopoietic stem cell transplantation. We report here two cases of SCID. …”
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Fabrication and Characterization of CuInSe2 Thin Film Applicable for a Solar Energy Light Absorption Material via a Low Temperature Solid State Reaction by Kuo-Chin Hsu, Yaw-Shyan Fu, Pei-Ying Lin, I-Tseng Tang, Jiunn-Der Liao

“…The CuInSe2 thin film prepared from the solid state reaction did not use the selenide process; its band gap might reach 1.06 eV, which is competent and suitable to be used for a thin film solar cell light absorption layer.…”
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Research Progress of Different Types of Microorganisms Passivation and Fixation of Cadmium Contamination by Ruihong CHEN, Yongli QIN, Feng LAI, Yongrong JIANG

“…Here is a review to summarize the mechanism of microbial passivation and fixation of cadmium based on the passivation and immobilization of typical strains and heavy metal cadmium: (1) Microbial cell walls and extracellular polymers (EPS) have abundant groups, such as carboxyl, hydroxyl, etc., which can effectively adsorb Cd2+, and passivated into crystalline minerals. (2) Sulfate-reducingbacteria, phosphate-dissolving bacteria, urease-producing bacteria in the environment can generate metal precipitates through their own metabolism, and then form crystal minerals through changes in the microenvironment. …”
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PluxI mutants with different promoting period and their application for quorum sensing regulated protein expression by Zhuoning Cao, Zhen Liu, Guilin Zhang, Xiangzhao Mao

“…Quorum sensing (QS) system can dynamically control the expression of proteins along with the cell growth. The promoting period of QS system has been little focused on until now. …”
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Physical Properties of Electrode Materials of Rechargeable Lithium Ion Batteries via DFT Calculations by Zeinab Saeedi Pour, Jaafar Jalilian, Seiyed Hamid Reza Shojaei

“…The geometry of the unit cell was optimized in triclinic and monoclinic phases. …”
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Stage-specific modulation of Drosophila gene expression with muscle GAL4 promoters by Ziwei Zhao, Erika R Geisbrecht

“…Defining precise gene expression patterns for tissues and/or cell types under GAL4 control will continue to evolve to suit experimental needs. …”
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Unwanted disorders and xenogeneic graft‐versus‐host disease in experimental immunodeficient mice: How to evaluate and how to report by Seyed Mostafa Monzavi, Samad Muhammadnejad, Vahid Mansouri, Hami Ashraf, Naser Ahmadbeigi

“…Generating animal models bearing tumors of human origin, such as patient‐derived or cell line–derived xenograft tumors, is dependent on immunodeficient strains. …”
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Binder-Free Hexagonal Boron Nitride Nanosheets (BNNSs) as Protective Coatings for Copper, Steel, and Wood: A Review by Muhammad Faheem Maqsood, Syed Muhammad Zain Mehdi, Arslan Ashraf, Umair Azhar, Naseem Abbas, Muhammad Asim Raza, Mohammed Amer

“…Due to their impermeability, boron nitride nanosheets (BNNSs) do not form a galvanic cell with the underlying metals, enhancing their effectiveness as protective coatings. …”
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Analysis of KRAS Mutations of Exon 2 Codons 12 and 13 by SNaPshot Analysis in Comparison to Common DNA Sequencing by Rica Zinsky, Servet Bölükbas, Holger Bartsch, Joachim Schirren, Annette Fisseler-Eckhoff

“…We examined KRAS mutations in exon 2 codons 12 and 13 with DNA sequencing and SNaPshot analysis in 100 formalin-fixed paraffin-embedded tumor tissue samples of pancreatic carcinoma, colorectal carcinoma, and nonsmall cell lung cancer specimens of the primary tumor or metastases. 40% of these samples demonstrated mutated KRAS genes using sequencing and SNaPshot-analysis; additional five samples (45/100) were identified only with the SNaPshot. …”
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