Proteomic and serologic assessments of responses to mRNA-1273 and BNT162b2 vaccines in human recipient sera by Thomas E. Hickey, Uma Mudunuri, Heidi A. Hempel, Troy J. Kemp, Nancy V. Roche, Keyur Talsania, Brian A. Sellers, James M. Cherry, Ligia A. Pinto

“…Thirty markers that contributed most to the model were associated with complement cascade and activation; IL-17, TNFR pro-apoptotic, and PI3K signaling; and cell cycle progression.DiscussionThese results demonstrate the utility of proteomics to evaluate correlates or predictors of serological responses to SARS-CoV-2 vaccination.…”
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Long-term increase in soluble interleukin-6 receptor levels in convalescents after mild COVID-19 infection by Juliane Lokau, Juliane Lokau, Yvonne Garbers, Manuel M. Vicente, Anna Dittrich, Stefan Meltendorf, Holger Lingel, Anja K. Münster-Kühnel, Monika Brunner-Weinzierl, Christoph Garbers, Christoph Garbers

“…Furthermore, we used single cell RNA sequencing data in order to determine which immune cell types are sources and targets of the individual cytokines and whether their expression is altered in severe COVID-19 patients.ResultsWe find that sIL-6R levels are not only increased in acute severely ill patients, but also in convalescents after a mild COVID-19 infection. …”
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Identification of common and specific fibrosis-related genes in three common chronic kidney diseases by Zhangning Fu, Xiaodong Geng, Chao Liu, Wanjun Shen, Zheyi Dong, Guannan Sun, Guangyan Cai, Xiangmei Chen, Quan Hong

“…Quantitative real-time PCR (qRT-PCR) was performed to validate the expression of the selected genes.Results Protein tyrosine phosphatase receptor type C (PTPRC), intercellular cell adhesion molecule-1 (ICAM1), vascular cell adhesion molecule-1 (VCAM1), interleukin 10 receptor alpha (IL10RA) and CC chemokine receptor 2 (CCR2) were identified as the potential common genes for kidney fibrosis in different types of CKD, while peroxisome proliferator-activated receptor alpha (PPARA), lactate oxidase (LOX), secreted phosphoprotein 1 (SPP1) were identified as the specific fibrosis-associated genes for DN, IgAN and MN respectively. qRT-PCR demonstrated that the expression levels of these selected genes were consistent with the NanoString analysis.Conclusions There were both commonalities and differences in the mechanisms of fibrosis in different types of CKD, the commonalities might be used as the common therapeutic targets for kidney fibrosis in CKD, while the differences might be used as the diagnostic markers for DN, IgAN and MN respectively. …”
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DEPDC1B, CDCA2, APOBEC3B, and TYMS are potential hub genes and therapeutic targets for diagnosing dialysis patients with heart failure by Wenwu Tang, Wenwu Tang, Zhixin Wang, Xinzhu Yuan, Liping Chen, Haiyang Guo, Zhirui Qi, Ying Zhang, Xisheng Xie

“…In addition, we further explored potential mechanism and function of hub genes in HF of patients with MHD through GSEA, immune cell infiltration analysis, drug analysis and establishment of molecular regulatory network.ResultsTotally 23 candidate genes were screened out by overlapping 673 differentially expressed genes (DEGs) and 147 key module genes, of which four hub genes (DEPDC1B, CDCA2, APOBEC3B and TYMS) were obtained by two machine learning algorithms. …”
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New evidence on the nephrotoxicity of fine particulate matter: Potential toxic components from different emission sources by Fu-Jen Cheng, Chien-Er Huang, Pei-Shih Chen, Yu-Lun Tseng, Chung-Shin Yuan, Ching-Shu Lai

“…PM2.5 water extracts from both sites induced inflammatory cytokine upregulation in the lungs and kidneys, and inflammatory cell infiltration, antioxidant activity downregulation, and elevated levels of kidney injury molecule 1 in the kidneys. …”
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Vitamin K‐dependent gamma‐carboxyglutamic acid protein 1 promotes pancreatic ductal adenocarcinoma progression through stabilizing oncoprotein KRAS and tyrosine kinase receptor EGF... by Zheng Wu, Qing Ye, Shan Zhang, Li‐Peng Hu, Xiao‐Qi Wang, Lin‐Li Yao, Lei Zhu, Shu‐Yu Xiao, Zong‐Hao Duan, Xue‐Li Zhang, Shu‐Heng Jiang, Zhi‐Gang Zhang, De‐Jun Liu, Dong‐Xue Li, Xiao‐Mei Yang

“…PRRG1 knockdown reduced PDAC cell proliferation, anchorage‐independent growth in vitro, and tumor growth in vivo. …”
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-Tyrosine Confers Residualizing Properties to a -Amino Acid-Rich Residualizing Peptide for Radioiodination of Internalizing Antibodies by Fook T. Lee PhD, Ingrid J. G. Burvenich PhD, Nancy Guo MBBS, MSc, Pece Kocovski BSc (Honors), Henri Tochon-Danguy PhD, Uwe Ackermann PhD, Graeme J. O’Keefe PhD, Sylvia Gong PhD, Angela Rigopoulos BSc, MSc, Zhanqi Liu MBBS, PhD, Hui K. Gan MBBS, FRACP, PhD, Andrew M. Scott MBBS, FRACP, MD

“…The radiochemical purities, antigen-expressing U87MG.de2-7 human glioblastoma cell-binding properties, and targeting of xenografts at 72 hours post injection of all radioconjugates were compared. …”
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Peptide-based vaccine targeting IL17A attenuates experimental spondyloarthritis in HLA-B27 transgenic rats by Tetsuya Tomita, Hiromi Rakugi, Hiroki Hayashi, Ryuichi Morishita, Jiao Sun, Yuka Yanagida, Shota Yoshida, Satoshi Baba, Akiko Tenma, Masayoshi Toyoura, Sotaro Kawabata, Takako Ehara, Ryoko Asaki, Makoto Sakaguchi, Hideki Tomioka, Munehisa Shimamura, Hironori Nakagami

“…The ELISpot assay showed that the IL17A peptide-based vaccine did not elicit any IL17A-reactive T cell responses. IL17A vaccine tends to mitigate, but not significant, in SpA treatment model. …”
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Merck Open Global Health Library in vitro screening against Schistosoma mansoni identified two new substances with antischistosomal activities for further development by Monique Evelyn Ueberall, Martina Berchthold, Cécile Häberli, Sven Lindemann, Thomas Spangenberg, Jennifer Keiser, Christoph G. Grevelding

“…The effects of the two lead structures were investigated in more detail by confocal laser scanning microscopy and 5-ethynyl 2´-deoxyuridine (EdU) assays to assess morphological effects and stem cell effects. Both substances showed negative effects on stem cell proliferation in S. mansoni but no further morphological changes. …”
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Molecular switch of the dendrite-to-spine transport of TDP-43/FMRP-bound neuronal mRNAs and its impairment in ASD by Pritha Majumder, Biswanath Chatterjee, Khadiza Akter, Asmar Ahsan, Su Jie Tan, Chi-Chen Huang, Jen-Fei Chu, Che-Kun James Shen

“…., immunoprecipitation (IP), RNA-IP, Immunofluorescence (IF)/fluorescence in situ hybridization (FISH), live cell imaging, live cell tracking of RNA using beacon, and mouse model study are used to elucidate a novel mechanism regulating dendritic spine transport of mRNAs in mammalian neurons. …”
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Integrating traditional biomarkers and emerging predictors to assess neoadjuvant chemotherapy efficacy in breast cancer: a multifactorial analysis of Ki-67, CDK4, EGFR, TILs and ct... by Tianzhao Du, Ye Yuan, Shulan Sun, Zhichao Gao, Xiaoshuai Li

“…Abstract Objective This study aimed to analyse the correlation between the expression of cell proliferation-associated antigen (Ki-67), cell cycle protein-dependent kinase 4 (CDK4), epidermal growth factor receptor (EGFR), tumour-infiltrating lymphocytes (TILs) and circulating tumour DNA (ctDNA) with the outcome and prognosis of patients with breast cancer (BC) undergoing neoadjuvant chemotherapy (NACT). …”
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Revealing novel protein interaction partners of glyphosate in Escherichia coli by Alix Sarah Aldehoff, Dominique Türkowsky, Patrick Lohmann, Masun Nabhan Homsi, Ulrike Rolle–Kampczyk, Elke Ueberham, Jörg Lehmann, Martin von Bergen, Nico Jehmlich, Sven–Bastiaan Haange

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Does diabetes modify the triglyceride–glucose index associated with cardiovascular events and mortality? A meta-analysis of 50 cohorts involving 7,239,790 participants by Jun Zhang, Qiye Zhan, Zhihao Deng, Ling Lin, Zhaolan Feng, Huabin He, Deju Zhang, Huilei Zhao, Xiang Gu, Xiaoping Yin, Peng Yu, Xiao Liu

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Comparison between traditional logistic regression and machine learning for predicting mortality in adult sepsis patients by Hongsheng Wu, Biling Liao, Tengfei Ji, Keqiang Ma, Yumei Luo, Shengmin Zhang

“…Model validation and comparison with the Sequential Organ Failure Assessment (SOFA) and the Acute Physiology and Chronic Health Evaluation (APACHE) scores were performed using receiver operating characteristic (ROC) curves, calibration curves, and decision curve analysis (DCA) curves in the validation set.ResultsUnivariate analysis was employed to assess 17 variables, namely gender, history of coronary heart disease (CHD), systolic pressure, white blood cell (WBC), neutrophil count (NEUT), lymphocyte count (LYMP), lactic acid, neutrophil-to-lymphocyte ratio (NLR), red blood cell distribution width (RDW), interleukin-6 (IL-6), prothrombin time (PT), international normalized ratio (INR), fibrinogen (FBI), D-dimer, aspartate aminotransferase (AST), total bilirubin (Tbil), and lung infection. …”
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Genetic profiling of multidrug-resistant Acinetobacter baumannii from a tertiary care center in Malaysia by Aisyah Syakirah Shahari, Navindra Kumari Palanisamy, Fadzilah Mohd Nor

“…These STs are associated with acquired blaOXA-23, indicating an evolutionary adaptation of A. baumannii within the hospital setting.IMPORTANCEAcinetobacter baumannii is a ubiquitous Gram-negative coccobacillus bacterium that is primarily associated with nosocomial infections that can colonize biotic and abiotic surfaces to enhance cell-to-cell adhesion, ensuring the establishment of infections. …”
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A murine model of Trypanosoma brucei-induced myocarditis and cardiac dysfunction by Nathan P. Crilly, Marcelle Dina Zita, Alexander K. Beaver, Polina Sysa-Shah, Aashik Bhalodia, Kathy Gabrielson, Luigi Adamo, Monica R. Mugnier

“…On flow cytometry, myocarditis was associated with an increase of most myocardial immune cell populations, including multiple T cell and macrophage subsets, corroborating the notion that T. brucei-associated cardiac damage is an immune-mediated event. …”
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Determining the status of tertiary lymphoid structures in invasive pulmonary adenocarcinoma based on chest CT radiomic features by Ye Yu, Tianshu Yang, Pengfei Ma, Yan Zeng, Yongming Dai, Yicheng Fu, Aie Liu, Ying Zhang, Guanglei Zhuang, Yan Zhou, Huawei Wu

“…Key Points TLSs are closely related to favorable clinical outcomes in non-small cell lung cancer. Radiomics from Chest CT predicted TLSs in patients with IA. …”
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Amantadine modulates novel macrophage phenotypes to enhance neural repair following spinal cord injury by Shijie Yang, Beibei Yu, Qing Zhang, Yongfeng Zhang, Longhui Fu, Bisheng Zhou, Haining Wu, Jianzhong Li, Shouping Gong

“…Methods We employed microarray and single-cell RNA sequencing (scRNA-seq) to investigate gene expression changes and immune cell dynamics in mice following crush injury at 3 and 7 days post-injury (dpi). …”
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MLinvitroTox reloaded for high-throughput hazard-based prioritization of high-resolution mass spectrometry data by Katarzyna Arturi, Eliza J. Harris, Lilian Gasser, Beate I. Escher, Georg Braun, Robin Bosshard, Juliane Hollender

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Phenotypic Identification of Blood Groups in Blood Donors: A Peruvian Multicenter Analysis by Yovera-Ancajima CDP, Calderon Cumpa LY, Lezama-Cotrina ID, Walttuoni-Picón E, Cárdenas-Mendoza WW, Culqui-García JE, Retuerto-Salazar WR, Céspedes Poma RC

“…Hospital Nacional Guillermo Almenara Irigoyen, Lima, Perú; 6Instituto de Estandarización En Laboratorio Clínico Del Perú, Lima, PerúCorrespondence: Eder Walttuoni-Picón, Email 2020008671@unfv.edu.peBackground: Red blood cell alloimmunization currently continues to be a significant problem during the blood transfusion process, where phenotypic identification plays a clinically relevant role in its prevention. …”
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