Gas-phase fractionation DDA promotes in-depth DIA phosphoproteome analysis by Zhiwei Tu, Yabin Li, Shuhui Ji, Shanshan Wang, Rui Zhou, Gertjan Kramer, Yu Cui, Fei Xie

“…Two GPF-DDA injections proved to increase 18 % phosphopeptide and 13 % phosphosite identification in HEK293 cell lines, while five injections resulted in up to 28 % phosphopeptide and 21 % phosphosite increases compared to library-free DIA analysis alone. …”
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Evolution of Treatment Outcomes and Prognostic Factors in Esophageal Cancer Surgery: A Retrospective Analysis of 1500 Consecutive Esophagostomies by Yasue Kimura, MD, PhD, Eiji Oki, MD, PhD, Tomonori Nakanoko, MD, PhD, Qingjiang Hu, MD, PhD, Keita Natsugoe, MD, Sho Nanbara, MD, PhD, Ryota Nakanishi, MD, PhD, Yuichiro Nakashima, MD, PhD, Mitsuhiko Ota, MD, PhD, Tomoharu Yoshizumi, MD, PhD

“…The incidences of squamous cell carcinoma and adenocarcinoma were 93.8% and 3.3%, respectively. …”
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Comparative analysis of clinical characteristics and outcomes between carbapenem-resistant and carbapenem-sensitive Klebsiella pneumoniae infections: insights from a tertiary hospi... by Bu Wang, Bu Wang, Wei Zhang, Hongxia Zhang, Maochen Li, Zhihua Zhang, Xiaocui Peng, Na Wang, Ning Song

“…CRKP patients had significantly higher body temperature, white blood cell counts, and inflammatory markers, while showing lower diastolic blood pressure and oxygen saturation (p < 0.05). …”
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Comprehensive analysis of mRNA and miRNA differential expression profiles in the hypothalamus-pituitary-gonadal axis in laying and broodiness period of Wanxi white geese by Xinwei Tong, Xiaojin Li, Yuhua Wang, Fei Xie, Ruidong Li, Man Ren, Qianqian Hu, Shenghe Li

“…Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analysis showed that differentially expressed genes (DEGs) and differentially expressed miRNAs (DEMs) were significantly enriched in hormone level regulation, cell communication, calcium signaling pathway, GnRH signaling pathway, MAPK signaling pathway, Wnt signaling pathway, and other processes. …”
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Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris by Pavel Borsky, Zdenek Fiala, Ctirad Andrys, Martin Beranek, Kvetoslava Hamakova, Andrea Malkova, Tereza Svadlakova, Jan Krejsek, Vladimir Palicka, Lenka Borska, Vit Rehacek

“…They are the markers of cell destructive processes. Objective. The aim of the present study was to evaluate the suitability of selected alarmins (HMGB1, IL-33, S100A7, and S100A12) as potential biomarkers of severity of psoriasis and to explore possible relationships between these proteins for the purpose of better understanding their roles in the immunopathology of psoriasis. …”
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Identification of novel pQTL‐SNPs associated with lung adenocarcinoma risk: A multi‐stage study by Yutong Wu, Huiwen Xu, Liping Mao, Rongrong Zhao, Junfeng Chu, Lili Huang, Wendi Zhang, Yiran Liu, Qiong Chen, Xiaobo Tao, Siqi Li, Shenxuan Zhou, Anhui Ning, Zhenyu Li, Tian Tian, Lei Zhang, Jiahua Cui, Guangyu Tian, Minjie Chu

“…In addition, the expression of NRP1 and APOH were significant decreased in LUAD cell lines and validated in plasma of LUAD patients. …”
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Guttiferone K Exerts the Anti-inflammatory Effect on Mycobacterium Tuberculosis- (H37Ra-) Infected Macrophages by Targeting the TLR/IRAK-1 Mediated Akt and NF-κB Pathway by Qingwen Zhang, Jinxia Sun, Yan Fu, Weigang He, Yinhong Li, Hongsheng Tan, Hongxi Xu, Xin Jiang

“…Guttiferone K could also suppress the NLRP3 inflammasome activity and induce autophagy by inhibiting the protein kinase B (p-Akt) and mammalian target of rapamycin (mTOR) phosphorylation at Ser473 and Ser2448 in both cell lines. Thus, Guttiferone K possesses significant anti-inflammatory effect, alleviating Mtb-induced inflammation with an underlying mechanism that targeting on the TLR/IRAK-1 pathway and inhibiting the downstream NF-κB and Akt/mTOR signaling pathways. …”
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Mechanistic study on the mitigation of cadmium accumulation in Ligusticum sinense cv. Chuanxiong through plant growth-promoting rhizobacteria Arthrobacter sp. CX-2 by Shu-qi Niu, Ting Li, Lin Liu, Xiu-wen Bao, Xing-mao Yang, Hao-ran Song, Yang Li, Jing Bai, Li-ying He, Qing-he Wang, Si-jing Liu, Jin-lin Guo

“…Chuanxiong were not affected by the addition of CX-2, which was confirmed through cell experiments. For rhizosphere soil, CX-2 significantly decreased the exchangeable Cd content by 36.84 % and enhanced the availability of nutrients. …”
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Differences in soil Cd immobilization and blockage of rice Cd uptake by biochar derived from crop residue and bone − A 2-year field experiment by Zhongjun Xue, Fengfeng Sui, Yanjie Qi, Siyu Pan, Ning Wang, Rongjun Bian, Stephen Joseph, Xuhui Zhang, Lianqing Li, Genxing Pan

“…Higher Si content in rice root in RHB treatment may promote complexation and deposition of Si hemicellulose-bound Cd in the root cell walls. This study reveals the important role of biochar's elemental composition and properties in soil Cd immobilization and the mitigation of rice Cd uptake.…”
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Signatures of H3K4me3 modification predict cancer immunotherapy response and identify a new immune checkpoint-SLAMF9 by Tao Fan, Chu Xiao, Ziqin Deng, Shuofeng Li, He Tian, Yujia Zheng, Bo Zheng, Chunxiang Li, Jie He

“…The mice melanoma model showed Slamf9 knockdown remarkably restrained cancer progression and enhanced the efficacy of anti-CTLA-4 and anti-PD-L1 therapies by elevating CD8 + T cell infiltration. This study provided a new H3K4me3-associated biomarker system to predict tumor immunotherapy response and suggested the preclinical rationale for investigating the roles of SLAMF9 in cancer immunity regulation and treatment.…”
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Leveraging Autofluorescence for Tumor Detection, Diagnosis, and Accurate Excision with Surgical Margin Assessment in Tumor Excision by Antonis Perdiou, Ramona Dumitrescu, Daniela Jumanca, Octavia Balean, Ruxandra Sava-Rosianu, Serban Talpos, Dacian Virgil Lalescu, Atena Galuscan

“…A double analysis was conducted: a primary analysis of the full cohort and a subgroup analysis focusing on squamous cell carcinoma (control: <i>n</i> = 19; experimental: <i>n</i> = 24). …”
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Associations between brominated flame retardants exposure and non-alcoholic fatty liver disease: Mediation analysis in the NHANES by Yulan Cheng, Jingyi Su, Xiangdong Wang, Ruiyao Huang, Zixuan Zhao, Kai Tian, Tianxiang Gu, Xiaoke Wang, Lin Chen, Xinyuan Zhao

“…Additionally, mediation analysis showed that absolute neutrophil cell count (ANC) and lymphocyte count (LC) (inflammation markers) have significantly mediated influences on the correlations of PBB153, PBDE85, and PBDE28 with N AFLD risk. …”
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Cadmium and polyvinyl chloride microplastics induce mitochondrial damage and apoptosis under oxidative stress in duck kidney by Hui Zou, Jie Song, Xianzu Luo, Waseem Ali, Sifan Li, Ling Xiong, Yan Chen, Yan Yuan, Yonggang Ma, Xishuai Tong, Zongping Liu

“…These injuries can induce mitochondrial damage and eventually lead to renal cell apoptosis. To sum up, these evidence show that Cd or PVC-MPs can induce kidney oxidative damage, trace element metabolism disorder, mitochondrial damage and apoptosis.…”
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Molecular genetic diagnosis and surgical management in a cohort of children with 46,XY disorders/differences of sex development by Yuenshan Sammi Wong, Yuenshan Sammi Wong, Ho Ming Luk, Ho Chung Yau, Lap Ming Wong, Sarah Wing Yiu Poon, Joanna Yuet Ling Tung, Yuk Him Tam, Yuk Him Tam

“…A total of 5/21 had germ cell neoplasms in one or both gonads. A total of 8/10 patients with gonadal dysgenesis had comorbidities involving other systems. …”
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The promotion effect of FeS2 on Sb2S3 bioleaching and Sb speciation transformation by Xing-fu Zheng, Xing-fu Zheng, Xing-fu Zheng, Jin-lan Xia, Jin-lan Xia, Zhen-yuan Nie, Zhen-yuan Nie, Hong-peng Cao, Hong-peng Cao, Rui-Jia Hu, Yu-ting Liang, Hong-chang Liu, Hong-chang Liu

“…The electrochemical results revealed that after FeS2 was mixed, a FeS2-Sb2S3 galvanic cell formed, which promoted the electron transfer efficiency and redox reaction of Sb2S3. …”
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Multi-omics unveils strain-specific neuroactive metabolite production linked to inflammation modulation by Bacteroides and their extracellular vesicles by Basit Yousuf, Walid Mottawea, Galal Ali Esmail, Nazila Nazemof, Nour Elhouda Bouhlel, Emmanuel Njoku, Yingxi Li, Xu Zhang, Zoran Minic, Riadh Hammami

“…We used CRISPR/Cas12a-based gene engineering to create a knockout mutant lacking the glutamate decarboxylase gene (gadB) to demonstrate the specific contribution of Bacteroides finegoldii-derived GABA in modulating intestinal homeostasis. Cell-free supernatants from wild-type (WT, GABA+) and ΔgadB (GABA-) provided GABA-independent reinforcement of epithelial membrane integrity in LPS-treated Caco-2/HT29-MTX co-cultures. …”
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Coagulation Profile of Convalescent Plasma Donors and Recipients by Hanna H Pitkänen MD, PhD, Tuukka Helin MD, PhD, Tamim Khawaja MD, Jukka-Pekka Pietilä MD, Mikael Kajova MD, Hanna Välimaa MD, PhD, DDS, Tero Vahlberg MSc, Jarkko Ihalainen MD, Antti Vierikko MD, Olli Vapalahti MD, PhD, Anu Kantele MD, PhD, Riitta Lassila MD, PhD

“…CP donors had normal blood cell counts and coagulation profiles, without differences between LCP and HCP donors at the baseline. …”
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Hydrogen Sulfide Promotes Functional Endometrium Recovery via Regulating Pyroptosis in Severe Endometrial Injury: A Prospective Laboratory Based Randomized Control Trial by Haiyan Shan, Mingyuan Xu, Jie Ni, Ling Zhou, Mengchun Xue, Jun Xue, Xiaohuan Yuan, Luyang Tao, Mingyang Zhang, Hong Zhang

“…Pyroptosis, a form of programmed cell death associated with inflammation, is initiated by the cleavage of gasdermin family proteins by caspase, which has been implicated in endometrial injury. …”
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Transcriptomic analysis of melatonin on the mechanism of embryonic gonadal development in female Jilin white geese by Jin Yu, Ichraf Mabrouk, Qiuyuan Liu, Yuxuan Zhou, Yupu Song, Jingyun Ma, Fengshuo Liu, Xiangman Hu, Zhiyi Yang, Yuxia Zeng, Jingtao Hu, Yongfeng Sun

“…These DEGs were enriched in Gene Ontology (GO) terms related to the microtubule cytoskeleton, cytoskeleton, cell adhesion, and biological adhesion and the KEGG enrichment analysis suggested that the DEGs were mainly enriched in “MAPK signaling pathway”, “Wnt signaling pathway”, “Calcium signaling pathway”, “Focal adhesion”, “Neuroactive ligand-receptor interaction”, “Melanogenesis”, “mTOR signaling pathway”, “ECM-receptor interaction”, and “TGF-beta signaling pathway” in the two comparison groups. …”
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EGR1 mRNA expression levels and polymorphisms are associated with slaughter performance in chickens by Mao Ye, Xiaohuan Chao, Chutian Ye, Lijin Guo, Zhexia Fan, Xuerong Ma, Aijun Liu, Weiming Liang, Shuya Chen, Cheng Fang, Xiquan Zhang, Qingbin Luo

“…We used various experimental methods, including RT-qPCR, Cell Counting Kit 8, 5-ethynyl-2′-deoxyuridine, western blot, flow cytometry, and immunofluorescence, to validate EGR1’s role in chicken primary myoblasts. …”
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